Kinesin and dynein-dynactin at intersecting microtubules: motor density affects dynein function.

abstract：:We present a two-component coarse-grained molecular-dynamics model for simulating the erythrocyte membrane. The proposed model possesses the key feature of combing the lipid bilayer and the erythrocyte cytoskeleton, thus showing both the fluidic behavior of the lipid bilayer and the elastic properties of the erythrocy...

journal_title：Biophysical journal

authors： Li H,Lykotrafitis G

更新日期：2012-01-04 00:00:00

abstract：:Loss of myelin in the central nervous system (CNS) leads to debilitating neurological deficits. High-resolution optical imaging of myelin in the CNS of animal models is limited by a lack of in vivo myelin labeling strategies. We demonstrated that third harmonic generation (THG) microscopy-a coherent, nonlinear, dye-fr...

journal_title：Biophysical journal

authors： Farrar MJ,Wise FW,Fetcho JR,Schaffer CB

更新日期：2011-03-02 00:00:00

abstract：:For proteins of known structure, the relative enthalpic stability with respect to wild-type, ΔΔH(U), can be estimated by direct computation of the folded and unfolded state energies. We propose a model by which the change in stability upon mutation can be predicted from all-atom molecular dynamics simulations for the ...

journal_title：Biophysical journal

authors： Dadarlat VM,Gorenstein LA,Post CB

更新日期：2012-10-17 00:00:00

abstract：:It is well known that electromagnetic fields cause mechanical forces. If one applies an electrical field to a suspension of microscopic particles, these particles realign themselves along the direction of the field and form pearl-chain-like aggregates. These chains are mostly single stranded but they are frequently mu...

journal_title：Biophysical journal

authors： Takashima S,Schwan HP

更新日期：1985-04-01 00:00:00

abstract：:The average sizes of fluid and gel domains in the two-component, two-phase system formed from mixtures of dimyristoyl phosphatidylcholine and distearoyl phosphatidylcholine were determined from an analysis of the electron spin resonance spectral lineshapes of a dimyristoyl phosphatidylcholine-nitroxide spin label as a...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Thompson TE

更新日期：1992-08-01 00:00:00

abstract：:The influence of basic open-loop synaptic connections on the firing of simultaneously recorded neurons has been investigated with auto- and cross-correlation histograms, using experimental records and computer simulations. The basic connections examined were direct synaptic excitation, direct synaptic inhibition, and ...

journal_title：Biophysical journal

authors： Moore GP,Segundo JP,Perkel DH,Levitan H

更新日期：1970-09-01 00:00:00

abstract：:Binding of a small molecule to a macromolecular target reduces its conformational freedom, resulting in a negative entropy change that opposes the binding. The goal of this study is to estimate the configurational entropy change of two minor-groove-binding ligands, netropsin and distamycin, upon binding to the DNA dup...

journal_title：Biophysical journal

authors： Dolenc J,Baron R,Oostenbrink C,Koller J,van Gunsteren WF

更新日期：2006-08-15 00:00:00

abstract：:Gigaohm recordings have been made from glutamate receptor channels in excised, outside-out patches of collagenase-treated locust muscle membrane. The channels in the excised patches exhibit the kinetic state switching first seen in megaohm recordings from intact muscle fibers. Analysis of channel dwell time distributi...

journal_title：Biophysical journal

authors： Bates SE,Sansom MS,Ball FG,Ramsey RL,Usherwood PN

更新日期：1990-07-01 00:00:00

abstract：:A new approach to the calculation of bimolecular association constants for partially diffusion-limited reactions between asymmetric species (e.g. the ligand binding site of a macromolecule covers only a portion of its surface) is presented. The usual formulation, which is almost always analytically intractable, is bas...

journal_title：Biophysical journal

authors： Shoup D,Lipari G,Szabo A

更新日期：1981-12-01 00:00:00

abstract：:Diacylglycerol, a biological membrane second messenger, is a strong perturber of phospholipid planar bilayers. It converts multibilayers to the reverse hexagonal phase (HII), composed of highly curved monolayers. We have used x-ray diffraction and osmotic stress of the HII phase to measure structural dimensions, spont...

journal_title：Biophysical journal

authors： Leikin S,Kozlov MM,Fuller NL,Rand RP

更新日期：1996-11-01 00:00:00

abstract：:A challenge in the understanding of creatine kinase (CK) fluxes reflected by NMR magnetization transfer in the perfused rat heart is the choice of a kinetic model of analysis. The complexity of the energetic pathways, due to the presence of adenosine triphosphate (ATP)-inorganic phosphate (Pi) exchange, of metabolite ...

journal_title：Biophysical journal

authors： Joubert F,Hoerter JA,Mazet JL

更新日期：2001-12-01 00:00:00

abstract：:Thermal stability of proteins is crucial for both biotechnological and therapeutic applications. Rational protein engineering therefore frequently aims at increasing thermal stability by introducing stabilizing mutations. The accurate prediction of the thermodynamic consequences caused by mutations, however, is highly...

journal_title：Biophysical journal

authors： Seeliger D,de Groot BL

更新日期：2010-05-19 00:00:00

abstract：:The molecular cascade that controls switching of the direction of rotation of Escherichia coli flagellar motors is well known, but the conformational changes that allow the rotor to switch are still unclear. The signaling molecule CheY, when phosphorylated, binds to the C-ring at the base of the rotor, raising the pro...

journal_title：Biophysical journal

authors： Hosu BG,Berg HC

更新日期：2018-02-06 00:00:00

abstract：:The structural changes in the photoreaction cycle of bacteriorhodopsin, a light-driven proton pump, was investigated at a resolution of 7 angstroms by a time-resolved x-ray diffraction experiment utilizing synchrotron x rays from an undulator of SPring-8. The x-ray diffraction measurement system, used in coupling with...

journal_title：Biophysical journal

authors： Oka T,Inoue K,Kataoka M,Yagi N

更新日期：2005-01-01 00:00:00

abstract：:Stabilized intermediate redox states of cytochrome c are generated by radiolytic reduction of initially oxidized enzyme in glass matrices at liquid nitrogen temperature. In the intermediate states the heme group is reduced by hydrated electrons, whereas the protein conformation is restrained close to its oxidized form...

journal_title：Biophysical journal

authors： Cartling B

更新日期：1983-08-01 00:00:00

abstract：:Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of...

journal_title：Biophysical journal

authors： Fish DJ,Horne MT,Searles RP,Brewood GP,Benight AS

更新日期：2007-05-15 00:00:00

abstract：:Nerve growth factor (NGF) mimics play an important role for therapies that target the receptor tyrosine kinase A (trkA). The N-terminal fragment of the NGF (N-term@NGF) was previously demonstrated to be an important determinant for affinity and specificity in the binding to trkA. Here we use a variety of computational...

journal_title：Biophysical journal

authors： Berrera M,Cattaneo A,Carloni P

更新日期：2006-09-15 00:00:00

abstract：:BP100 (KKLFKKILKYL-NH(2)) is a short cecropin A-melittin hybrid peptide, obtained through a combinatorial chemistry approach, which is highly effective in inhibiting both the in vitro and in vivo growth of economically important plant pathogenic Gram-negatives. The intrinsic Tyr fluorescence of BP100 was taken advanta...

journal_title：Biophysical journal

authors： Ferre R,Melo MN,Correia AD,Feliu L,Bardají E,Planas M,Castanho M

更新日期：2009-03-04 00:00:00

abstract：:The current-voltage (I-V) relation of the background current, IK1, was studied in isolated canine cardiac Purkinje myocytes using the whole-cell, patch-clamp technique. Since Ba2+ and Cs+ block IK1, these cations were used to separate the I-V relation of IK1 from that of the whole cell. The I-V relation of IK1 was mea...

journal_title：Biophysical journal

authors： Shah AK,Cohen IS,Datyner NB

更新日期：1987-10-01 00:00:00

abstract：:Oriented purple membranes were equilibrated under controlled (2)H(2)O relative humidity ranging from 15% to 93% and introduced into the magnetic field of an NMR spectrometer with the membrane normal parallel to the magnetic field direction. Deuterium solid-state NMR spectra of these samples resolved four deuteron popu...

journal_title：Biophysical journal

authors： Bechinger B,Weik M

更新日期：2003-07-01 00:00:00

abstract：:Alamethicin and its analogs from cation selective, multi-conductance channels in lipid bilayers. The conductance levels have been thought to be due to a barrel-stave structure where conducting pores (barrels) are formed by the self-assembly of a variable number of alpha-helical rods (staves). The conductance transitio...

journal_title：Biophysical journal

authors： Laver DR

更新日期：1994-02-01 00:00:00

abstract：:Cell migration is based on an actin treadmill, which in turn depends on recycling of G-actin across the cell, from the rear where F-actin disassembles, to the front, where F-actin polymerizes. To analyze the rates of the actin transport, we used the Virtual Cell software to solve the diffusion-drift-reaction equations...

journal_title：Biophysical journal

authors： Novak IL,Slepchenko BM,Mogilner A

更新日期：2008-08-01 00:00:00

abstract：:Downstream processes that discriminate between DNA adducts of a third generation platinum antitumor drug oxaliplatin and conventional cisplatin are believed to be responsible for the differences in their biological effects. These different biological effects are explained by the ability of oxaliplatin to form DNA addu...

journal_title：Biophysical journal

authors： Malina J,Novakova O,Vojtiskova M,Natile G,Brabec V

更新日期：2007-12-01 00:00:00

abstract：:The state of photosystem II core complex (PS II CC) in monolayer at the gas-water interface was investigated using in situ polarization-modulated infrared reflection absorption spectroscopy and x-ray reflectivity techniques. Two approaches for preparing and manipulating the monolayers were examined and compared. In th...

journal_title：Biophysical journal

authors： Gallant J,Desbat B,Vaknin D,Salesse C

更新日期：1998-12-01 00:00:00

abstract：:In this article, we explore the information content of molecular force-field calculations. We make use of exhaustive lattice models of molecular conformations and reduced alphabet sequences to determine the relative resolving power of pairwise interaction-based force fields. We find that sequence-specific interactions...

journal_title：Biophysical journal

authors： Aynechi T,Kuntz ID

更新日期：2005-11-01 00:00:00

abstract：:The orientational ordering of beta-carotene and crocetin embedded in lamellar model membranes has been investigated by angle-resolved resonance Raman scattering at a temperature well above the phase transition of the lipid chains. It is shown that the ordering of the carotenoids is dependent on the chemical compositio...

journal_title：Biophysical journal

authors： van de Ven M,Kattenberg M,van Ginkel G,Levine YK

更新日期：1984-06-01 00:00:00

abstract：:Human transthyretin (TTR) is an amyloidogenic protein. The pathway of TTR amyloid formation has been proposed based on lines of evidence: TTR tetramer first dissociates into native monomers, which is shown to be a rate-limiting step in the formation of fibrils. Subsequently, the monomeric species partially unfold to f...

journal_title：Biophysical journal

authors： Yang M,Lei M,Bruschweiler R,Huo S

更新日期：2005-07-01 00:00:00

abstract：:Diacylglycerol kinase ε (DGKε) is a membrane-bound enzyme that catalyzes the ATP-dependent phosphorylation of diacylglycerol to form phosphatidic acid (PA) in the phosphatidylinositol cycle. DGKε lacks a putative regulatory domain and has recently been reported to be regulated by highly curved membranes. To further st...

journal_title：Biophysical journal

authors： Bozelli JC Jr,Yune J,Hou YH,Chatha P,Fernandes A,Cao Z,Tong Y,Epand RM

更新日期：2020-02-25 00:00:00

abstract：:RNA functions are often kinetically controlled. The folding kinetics of RNAs involves global structural changes and local nucleotide movement, such as base flipping. The most elementary step in RNA folding is the closing and opening of a basepair. By integrating molecular dynamics simulation, master equation, and kine...

journal_title：Biophysical journal

authors： Wang F,Sun LZ,Cai P,Chen SJ,Xu X

更新日期：2019-11-05 00:00:00

abstract：:The viral genome and replicative enzymes of the human immunodeficiency virus are encased in a shell consisting of assembled mature capsid protein (CA). The core shell is a stable, effective protective barrier, but is also poised for dissolution on cue to allow transmission of the viral genome into its new host. In thi...

journal_title：Biophysical journal

authors： Ehrlich LS,Liu T,Scarlata S,Chu B,Carter CA

更新日期：2001-07-01 00:00:00