Kinetic Mechanism of RNA Helix-Terminal Basepairing-A Kinetic Minima Network Analysis.

abstract：:Widely disparate viruses enter the host cell through an endocytic pathway and travel the cytoplasm inside an endosome. For the viral genetic material to be delivered into the cytoplasm, these viruses have to escape the endosomal compartment, an event triggered by the conformational changes of viral endosomolytic prote...

journal_title：Biophysical journal

authors： Lagache T,Danos O,Holcman D

更新日期：2012-03-07 00:00:00

abstract：:The regulatory mechanism of sarcomeric activity has not been fully clarified yet because of its complex and cooperative nature, which involves both Ca(2+) and cross-bridge binding to the thin filament. To reveal the mechanism of regulation mediated by the cross-bridges, separately from the effect of Ca(2+), we investi...

journal_title：Biophysical journal

authors： Shimamoto Y,Kono F,Suzuki M,Ishiwata S

更新日期：2007-12-15 00:00:00

abstract：:The morphological features of α-synuclein (AS) amyloid aggregation in vitro and in cells were elucidated at the nanoscale by far-field subdiffraction fluorescence localization microscopy. Labeling AS with rhodamine spiroamide probes allowed us to image AS fibrillar structures by fluorescence stochastic nanoscopy with ...

journal_title：Biophysical journal

authors： Roberti MJ,Fölling J,Celej MS,Bossi M,Jovin TM,Jares-Erijman EA

更新日期：2012-04-04 00:00:00

abstract：:Recent studies have demonstrated that caffeine can act as an antimutagen and inhibit the cytoxic and/or cytostatic effects of some DNA intercalating agents. It has been suggested that this inhibitory effect may be due to complexation of the DNA intercalator with caffeine. In this study we employ optical absorption, fl...

journal_title：Biophysical journal

authors： Larsen RW,Jasuja R,Hetzler RK,Muraoka PT,Andrada VG,Jameson DM

更新日期：1996-01-01 00:00:00

abstract：:Human immunodeficiency virus-1 viral infectivity factor (Vif) is an intrinsically disordered protein responsible for the ubiquitination of the APOBEC3 (A3) antiviral proteins. Vif folds when it binds Cullin-RING E3 ligase 5 and the transcription cofactor CBF-β. A five-protein complex containing the substrate receptor ...

journal_title：Biophysical journal

authors： Ball KA,Chan LM,Stanley DJ,Tierney E,Thapa S,Ta HM,Burton L,Binning JM,Jacobson MP,Gross JD

更新日期：2019-04-23 00:00:00

abstract：:A discussion is given of the general role of diffusion in enzyme kinetics based upon a rigorous theory for bimolecular association and dissociation steps that was presented previously, and criteria are formulated for the dependence of the over-all rate on medium viscosity. With these criteria it is possible to conclud...

journal_title：Biophysical journal

authors： Schurr JM

更新日期：1970-08-01 00:00:00

abstract：:The structure of a membrane-embedded alpha-helical reference protein, the M13 major coat protein, is characterized under different conditions of hydrophobic mismatch using fluorescence resonance energy transfer in combination with high-throughput mutagenesis. We show that the structure is similar in both thin (14:1) a...

journal_title：Biophysical journal

authors： Vos WL,Schor M,Nazarov PV,Koehorst RB,Spruijt RB,Hemminga MA

更新日期：2007-11-15 00:00:00

abstract：:The phenomena reported are part of a study about the effects of ionizing radiation on membrane transport. We found that the conductance of lipid membranes in the presence of the polyene-antibiotics nystatin or amphotericin B is reduced to virtually zero following irradiation. Ion channels formed by these substances se...

journal_title：Biophysical journal

authors： Barth C,Stark G,Wilhelm M

更新日期：1993-01-01 00:00:00

abstract：:Cell adhesion to the extracellular matrix (ECM) is highly active and plays a crucial role in various physiological functions. The active response of cells to physicochemical cues has been universally discovered in multiple microenvironments. However, the mechanisms to rule these active behaviors of cells are still poo...

journal_title：Biophysical journal

authors： Fang Y,Gong H,Yang R,Lai KWC,Quan M

更新日期：2020-06-02 00:00:00

abstract：:A major component of green tea extracts, catechin (-)-Epigallocatechin gallate (EGCg), has been reported to be biologically active and interacting with membranes. A recent study reported drastic effects of EGCg on giant unilamellar vesicles (GUVs). In particular, EGCg above 30 microM caused GUVs to burst. Here we inve...

journal_title：Biophysical journal

authors： Sun Y,Hung WC,Chen FY,Lee CC,Huang HW

更新日期：2009-02-01 00:00:00

abstract：:Amperometry recording reveals the exocytosis of catecholamine from individual vesicles as a sequential process, typically beginning slowly with a prespike foot, accelerating sharply to initiate a spike, reaching a peak, and then decaying. This complex sequence reflects the interplay between diffusion, flux through a f...

journal_title：Biophysical journal

authors： Jackson MB,Hsiao YT,Chang CW

更新日期：2020-07-07 00:00:00

abstract：:The calyx of Held is a giant nerve terminal that forms a glutamatergic synapse in the auditory pathway. Due to its large size, it offers a number of advantages for biophysical studies, including voltage-clamp of both pre- and postsynaptic compartments and the loading with indicator dyes and caged compounds. Three aspe...

journal_title：Biophysical journal

authors： Neher E

更新日期：2017-01-24 00:00:00

abstract：:Entropy Sampling Monte Carlo (ESMC) simulations were carried out to study the thermodynamics of the folding transition in the GCN4 leucine zipper (GCN4-lz) in the context of a reduced model. Using the calculated partition functions for the monomer and dimer, and taking into account the equilibrium between the monomer ...

journal_title：Biophysical journal

authors： Mohanty D,Kolinski A,Skolnick J

更新日期：1999-07-01 00:00:00

abstract：:An equation of state for lipid monolayers at the air-water interface is presented, which takes into account the effects of the conformation and the number and position of double bonds of the hydrocarbon chains. The total Hamiltonian of the monolayer is assumed to consist of three terms. Two of them are calculated exac...

journal_title：Biophysical journal

authors： Feng SS,MacDonald RC

更新日期：1995-08-01 00:00:00

abstract：:The coronavirus disease 2019 (COVID-19) pandemic has swept over the world in the past months, causing significant loss of life and consequences to human health. Although numerous drug and vaccine development efforts are underway, there are many outstanding questions on the mechanism of severe acute respiratory syndrom...

journal_title：Biophysical journal

authors： Barros EP,Casalino L,Gaieb Z,Dommer AC,Wang Y,Fallon L,Raguette L,Belfon K,Simmerling C,Amaro RE

更新日期：2020-11-13 00:00:00

abstract：:TWIK-1 two-pore domain K(+) channels generally produce nonmeasurable or very low levels of K(+) currents in heterologous expression systems under physiologically ionic conditions. Two controversial mechanisms have been proposed to account for this behavior: TWIK-1 K(+) channels are expressed in the cell surface but si...

journal_title：Biophysical journal

authors： Ma L,Xie YP,Zhou M,Chen H

更新日期：2012-04-18 00:00:00

abstract：:Actin filaments are semiflexible polymers that display large-scale conformational twisting and bending motions. Modulation of filament bending and twisting dynamics has been linked to regulatory actin-binding protein function, filament assembly and fragmentation, and overall cell motility. The relationship between act...

journal_title：Biophysical journal

authors： De La Cruz EM,Roland J,McCullough BR,Blanchoin L,Martiel JL

更新日期：2010-09-22 00:00:00

abstract：:Here we extend the ability to predict hydrodynamic coefficients and other solution properties of rigid macromolecular structures from atomic-level structures, implemented in the computer program HYDROPRO, to models with lower, residue-level resolution. Whereas in the former case there is one bead per nonhydrogen atom,...

journal_title：Biophysical journal

authors： Ortega A,Amorós D,García de la Torre J

更新日期：2011-08-17 00:00:00

abstract：:The reported rates of thermal 13-cis to all-trans isomerization of the protonated Schiff base of retinal (PSBR) in solution and in bacteriorhodopsin (BR) are shown to be correlated with the red shift in the absorption maximum of the chromophore, though the linear fit is different for BR and for a model PSBR in solutio...

journal_title：Biophysical journal

authors： Milder SJ

更新日期：1991-08-01 00:00:00

abstract：:Beat-to-beat alternation of the action potential duration (APD) in paced cardiac cells has been linked to the onset of lethal arrhythmias. Both experimental and theoretical studies have shown that alternans at the single cell level can be caused by unstable membrane voltage (V(m)) dynamics linked to steep APD-restitut...

journal_title：Biophysical journal

authors： Sato D,Shiferaw Y,Qu Z,Garfinkel A,Weiss JN,Karma A

更新日期：2007-02-15 00:00:00

abstract：:Previous studies have demonstrated a calcium-dependent interaction of calmodulin (CaM) and Fas that is regulated during Fas-induced apoptosis in several cell lines, including cholangiocarcinoma, Jurkat cells, and osteoclasts. The binding of CaM and Fas has been identified on residues 231-254 of Fas; the V254N point mu...

journal_title：Biophysical journal

authors： Suever JD,Chen Y,McDonald JM,Song Y

更新日期：2008-12-15 00:00:00

abstract：:Motions through the energy landscape of proteins lead to biological function. At temperatures below a dynamical transition (150-250 K), some of these motions are arrested and the activity of some proteins ceases. Here, we introduce the technique of temperature-derivative fluorescence microspectrophotometry to investig...

journal_title：Biophysical journal

authors： Weik M,Vernede X,Royant A,Bourgeois D

更新日期：2004-05-01 00:00:00

abstract：:Cholesterol is a key component of eukaryotic membranes, but its role in cellular biology in general and in lipid rafts in particular remains controversial. Model membranes are used extensively to determine the phase behavior of ternary mixtures of cholesterol, a saturated lipid, and an unsaturated lipid with liquid-or...

journal_title：Biophysical journal

authors： Bennett WFD,Shea JE,Tieleman DP

更新日期：2018-06-05 00:00:00

abstract：:A useful technique in studying the saturation of hemoglobin in erythrocytes or myoglobin in tissue is cryophotometry, in which tissue is frozen for later spectrophotometric analysis. A general question associated with this technique is whether the freezing process alters the chemical state. This paper presents a theor...

journal_title：Biophysical journal

authors： Clark A Jr,Clark PA

更新日期：1983-04-01 00:00:00

abstract：:Twenty-seven mutant bacteriorhodopsin's were screened to determine the PKa for reversal of the permanent electric dipole moment. The photoelectric response of an aqueous purple-membrane suspension was used to determine the direction of the purple-membrane dipole moment as a function of pH. The pK(a) for the dipole rev...

journal_title：Biophysical journal

authors： Hsu KC,Rayfield GW,Needleman R

更新日期：1996-05-01 00:00:00

abstract：:Singlet-triplet mixing in the initial radical-pair state, P[unk]I[unk], of photosynthetic bacterial reaction centers is due to the hyperfine mechanism at low magnetic fields and both the hyperfine and Deltag mechanisms at high magnetic fields (>1 kG). Since the hyperfine field felt by the electron spins in P[unk]I[unk...

journal_title：Biophysical journal

authors： Goldstein RA,Boxer SG

更新日期：1987-06-01 00:00:00

abstract：:The subgel phases of unlabeled, specifically chain perdeuterated and specifically 13C = O labeled representative samples of the n-saturated diacylphosphatidylcholines were studied by Fourier-transform infrared spectroscopy. Our results indicate that the spectroscopic properties exhibited by the subgel phases of the lo...

journal_title：Biophysical journal

authors： Lewis RN,McElhaney RN

更新日期：1992-01-01 00:00:00

abstract：:Cationic antimicrobial cationic peptides (CAMP) have been found in recent years to play a decisive role in hosts' defense against microbial infection. They have also been investigated as a new therapeutic tool, necessary in particular due to the increasing resistance of microbiological populations to antibiotics. The ...

journal_title：Biophysical journal

authors： Howe J,Andrä J,Conde R,Iriarte M,Garidel P,Koch MH,Gutsmann T,Moriyón I,Brandenburg K

更新日期：2007-04-15 00:00:00

abstract：:Chemotactic effects of dissolved oxygen on motions of Escherichia coli in a motility buffer solution have been studied by measurements of quasielastic light scattering. Under conditions where the bacteria form a sharp band in an oxygen concentrations gradient created by their metabolism, components of motions along th...

journal_title：Biophysical journal

authors： Wang PC,Chen SH

更新日期：1981-10-01 00:00:00

abstract：:Recently a periplasmic glucose/galactose binding protein, GGRQ26C, immobilized on a gold surface has been used as an active part of a glucose biosensor based on quartz microbalance technique. However the nature of the glucose detection was not clear. Here we have found that the receptor protein film immobilized on the...

journal_title：Biophysical journal

authors： Sokolov I,Subba-Rao V,Luck LA

更新日期：2006-02-01 00:00:00