Abstract:
:Recently a periplasmic glucose/galactose binding protein, GGRQ26C, immobilized on a gold surface has been used as an active part of a glucose biosensor based on quartz microbalance technique. However the nature of the glucose detection was not clear. Here we have found that the receptor protein film immobilized on the gold surface increases its rigidity when glucose is added, which explains the unexpected detection signal. To study the rigidity change, we developed a new fast and simple method based on using atomic force microscopy (AFM) in tapping mode. The method was verified by explicit measurements of the Young's modulus of the protein film by conventional AFM methods. Since there are a host of receptors that undergo structural change when activated by ligand, AFM can play a key role in the development and/or optimization of biosensors based on rigidity changes in biomolecules.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Sokolov I,Subba-Rao V,Luck LAdoi
10.1529/biophysj.105.060442subject
Has Abstractpub_date
2006-02-01 00:00:00pages
1055-63issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(06)72294-9journal_volume
90pub_type
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