The role of diffusion in enzyme kinetics.

abstract：:The cardiac muscle protein titin, responsible for developing passive elasticity and extensibility of muscle, possesses about 40 immunoglobulin-like (Ig) domains in its I-band region. Atomic force microscopy (AFM) and steered molecular dynamics (SMD) have been successfully combined to investigate the reversible unfoldi...

journal_title：Biophysical journal

authors： Gao M,Wilmanns M,Schulten K

更新日期：2002-12-01 00:00:00

abstract：:Small angle x-ray and neutron scattering are techniques that give solution structures for large macromolecules. The creation of physically realistic atomistic models from known high-resolution structures to determine joint x-ray and neutron scattering best-fit structures offers a, to our knowledge, new method that sig...

journal_title：Biophysical journal

authors： Wright DW,Elliston ELK,Hui GK,Perkins SJ

更新日期：2019-12-03 00:00:00

abstract：:Analyses of receptor-ligand interactions are important to the understanding of cellular adhesion. Traditional methods of measuring the three-dimensional (3D) dissociation constant (Kd) require at least one of the molecular species in solution and hence cannot be directly applied to the case of cell adhesion. We descri...

journal_title：Biophysical journal

authors： Piper JW,Swerlick RA,Zhu C

更新日期：1998-01-01 00:00:00

abstract：:We present a molecular system where polymerization is controlled externally by tuning the elastic energy of the monomers. The elastic energy, provided by a DNA molecular spring, destabilizes the monomer state through a process analogous to domain swapping. This energy can be large (of approximately 10 kT) and thus dri...

journal_title：Biophysical journal

authors： Wang A,Zocchi G

更新日期：2009-03-18 00:00:00

abstract：:In contrast with most inhalational anesthetics, the anesthetic gases xenon (Xe) and nitrous oxide (N(2)O) act by blocking the N-methyl-d-aspartate (NMDA) receptor. Using x-ray crystallography, we examined the binding characteristics of these two gases on two soluble proteins as structural models: urate oxidase, which ...

journal_title：Biophysical journal

authors： Colloc'h N,Sopkova-de Oliveira Santos J,Retailleau P,Vivarès D,Bonneté F,Langlois d'Estainto B,Gallois B,Brisson A,Risso JJ,Lemaire M,Prangé T,Abraini JH

更新日期：2007-01-01 00:00:00

abstract：:Receptor activity modifying proteins (RAMPs) are a family of single-pass transmembrane proteins that dimerize with G-protein-coupled receptors. They may alter the ligand recognition properties of the receptors (particularly for the calcitonin receptor-like receptor, CLR). Very little structural information is availabl...

journal_title：Biophysical journal

authors： Simms J,Hay DL,Wheatley M,Poyner DR

更新日期：2006-07-15 00:00:00

abstract：:The observation of biological processes at the molecular scale in real time requires high spatial and temporal resolution. Magnetic tweezers are straightforward to implement, free of radiation or photodamage, and provide ample multiplexing capability, but their spatiotemporal resolution has lagged behind that of other...

journal_title：Biophysical journal

authors： Dulin D,Cui TJ,Cnossen J,Docter MW,Lipfert J,Dekker NH

更新日期：2015-11-17 00:00:00

abstract：:Dual-color fluorescence cross-correlation analysis is a powerful tool for probing interactions of different fluorescently labeled molecules in aqueous solution. The concept is the selective observation of coordinated spontaneous fluctuations in two separate detection channels that unambiguously reflect the existence o...

journal_title：Biophysical journal

authors： Heinze KG,Rarbach M,Jahnz M,Schwille P

更新日期：2002-09-01 00:00:00

abstract：:The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic flash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that...

journal_title：Biophysical journal

authors： Gohon Y,Dahmane T,Ruigrok RW,Schuck P,Charvolin D,Rappaport F,Timmins P,Engelman DM,Tribet C,Popot JL,Ebel C

更新日期：2008-05-01 00:00:00

abstract：:Chimeras consisting of the homologous skeletal dihydropyridine receptor (DHPR) beta1a subunit and the heterologous cardiac/brain beta2a subunit were used to determine which regions of beta1a were responsible for the skeletal-type excitation-contraction (EC) coupling phenotype. Chimeras were transiently transfected in ...

journal_title：Biophysical journal

authors： Sheridan DC,Cheng W,Carbonneau L,Ahern CA,Coronado R

更新日期：2004-08-01 00:00:00

abstract：:We propose a general scheme for measuring the attraction between mechanically frustrated semiflexible fibers by measuring their thermal fluctuations and shape. We apply this analysis to a system of sickle hemoglobin (HbS) fibers that laterally attract one another. These fibers appear to "zip" together before reaching ...

journal_title：Biophysical journal

authors： Jones CW,Wang JC,Briehl RW,Turner MS

更新日期：2005-04-01 00:00:00

abstract：:Actin, a highly conserved cytoskeletal protein found in all eukaryotic cells, facilitates cell motility and membrane remodeling via a directional polymerization cycle referred to as treadmilling. The nucleotide bound at the core of each actin subunit regulates this process. Although the biochemical kinetics of treadmi...

journal_title：Biophysical journal

authors： Saunders MG,Tempkin J,Weare J,Dinner AR,Roux B,Voth GA

更新日期：2014-04-15 00:00:00

abstract：:Structures of truncated versions of the influenza A virus M2 proton channel have been determined recently by x-ray crystallography in the open conformation of the channel, and by NMR in the closed state. The structures differ in the position of the bound inhibitors. The x-ray structure shows a single amantadine molecu...

journal_title：Biophysical journal

authors： Chuang GY,Kozakov D,Brenke R,Beglov D,Guarnieri F,Vajda S

更新日期：2009-11-18 00:00:00

abstract：:Charge movement currents (IQ) and calcium transients (delta[Ca2+]) were measured simultaneously in frog skeletal muscle fibers, voltage clamped in a double vaseline gap chamber, using Antipyrylazo III as the calcium indicator. The rate of release of calcium from the SR (Rrel) was calculated from the calcium transients...

journal_title：Biophysical journal

authors： Simon BJ,Hill DA

更新日期：1992-05-01 00:00:00

abstract：:We have measured the kinetics of adsorption of small (12.5-nm radius) unilamellar vesicles onto SiO2, oxidized gold, and a self-assembled monolayer of methyl-terminated thiols, using a quartz crystal microbalance (QCM). Simultaneous measurements of the shift in resonant frequency and the change in energy dissipation a...

journal_title：Biophysical journal

authors： Keller CA,Kasemo B

更新日期：1998-09-01 00:00:00

abstract：:Galactosylceramides (GalCers) containing nervonoyl (24:1(Delta15(cis))) acyl chains have the capacity to assemble into nanotubular microstructures in excess water (. Biophys. J. 69:1976-1986). To define the structural parameters that modulate nanotube formation, GalCer derivatives were synthesized that contained cis m...

journal_title：Biophysical journal

authors： Kulkarni VS,Boggs JM,Brown RE

更新日期：1999-07-01 00:00:00

abstract：:Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although the crystal structures of dark- and light-adapted states have been determined, the molecular mechanisms underlying photoactivation remain elusive. Here, we demonstrate that the conserved tongue region of...

journal_title：Biophysical journal

authors： Gustavsson E,Isaksson L,Persson C,Mayzel M,Brath U,Vrhovac L,Ihalainen JA,Karlsson BG,Orekhov V,Westenhoff S

更新日期：2020-01-21 00:00:00

abstract：:Nonphotochemical quenching is the protective mechanism against overexcitation of photosystem II, triggered by excess ΔpH in photosynthetic membranes. The light-harvesting complexes (LHCs), the de-epoxidation of violaxanthin to zeaxanthin, and the photosystem II subunit S (PsbS) work in synergy for an optimized multile...

journal_title：Biophysical journal

authors： Daskalakis V,Papadatos S

更新日期：2017-12-05 00:00:00

abstract：:The geometry and mechanical properties of the human erythrocyte membrane cytoskeleton are investigated by a computer simulation in which the cytoskeleton is represented by a network of polymer chains. Four elastic moduli as well as the area and thickness are predicted for the chain network as a function of temperature...

journal_title：Biophysical journal

authors： Boal DH

更新日期：1994-08-01 00:00:00

abstract：:The effects of the anionic tungsten carbonyl complex [W(CO)(5)SC(6)H(5)](-) and its fluorinated analog [W(CO)(5)SC(6)F(5)](-) on the electrical properties of the plasma membrane of mouse myeloma cells were studied by the single-cell electrorotation technique. At micromolar concentrations, both compounds gave rise to a...

journal_title：Biophysical journal

authors： Kürschner M,Nielsen K,von Langen JR,Schenk WA,Zimmermann U,Sukhorukov VL

更新日期：2000-09-01 00:00:00

abstract：:Juxtacrine signaling is an important class of signaling systems that plays a crucial role in various developmental processes ranging from coordination of differentiation between neighboring cells to guiding axon growth during neurogenesis. Such signaling systems rely on the interaction between receptors on one cell an...

journal_title：Biophysical journal

authors： Yaron T,Cordova Y,Sprinzak D

更新日期：2014-11-18 00:00:00

abstract：:The serine protease inhibitor antithrombin undergoes extensive conformational changes during functional interaction with its target proteases. Changes include insertion of the reactive loop region into a beta-sheet structure in the protein core. We explore the possibility that these changes are linked to water transfe...

journal_title：Biophysical journal

authors： Liang J,McGee MP

更新日期：1998-08-01 00:00:00

abstract：:The thermal stability of a Schizolobium parahyba chymotrypsin inhibitor (SPCI) as a function of pH has been investigated using fluorescence, circular dichroism, and differential scanning calorimetry (DSC). The thermodynamic parameters derived from all methods are remarkably similar and strongly suggest the high stabil...

journal_title：Biophysical journal

authors： Teles RC,Calderon Lde A,Medrano FJ,Barbosa JA,Guimarães BG,Santoro MM,de Freitas SM

更新日期：2005-05-01 00:00:00

abstract：:Slow stretch ramps (velocity: 0.17 fiber lengths s-1) were imposed during fused tetanic contractions of intact muscle fibers of the frog (1.4-3.0 degrees C; sarcomere length: 2.12-2.21 microns). Instantaneous force-extension relations were derived both under isometric conditions and during slow stretch by applying fas...

journal_title：Biophysical journal

authors： Månsson A

更新日期：1989-08-01 00:00:00

abstract：:When a dissolved macromolecule is in chemical equilibrium with a free ionic species, the charge configuration, and hence the dipole vector, of the macromolecule is fluctuating. Expressions for the static dielectric constant and the relaxation spectrum of such a mixture are here derived in terms of the components of th...

journal_title：Biophysical journal

authors： Scheider W

更新日期：1965-09-01 00:00:00

abstract：:Eukaryotic DNA is packaged into the cell nucleus as a nucleoprotein complex, chromatin. Despite this condensed state, access to the DNA sequence must occur during gene expression and other essential genetic events. Here we employ optical tweezers stretching of reconstituted chromatin fibers to investigate the release ...

journal_title：Biophysical journal

authors： Pope LH,Bennink ML,van Leijenhorst-Groener KA,Nikova D,Greve J,Marko JF

更新日期：2005-05-01 00:00:00

abstract：:Our ability to understand speech requires neural tuning with high frequency resolution, but the peripheral mechanisms underlying sharp tuning in humans remain unclear. Sharp tuning in genetically modified mice has been attributed to decreases in spread of excitation of tectorial membrane traveling waves. Here we show ...

journal_title：Biophysical journal

authors： Farrahi S,Ghaffari R,Sellon JB,Nakajima HH,Freeman DM

更新日期：2016-09-06 00:00:00

abstract：:A sequence determinant of reduction potentials is reported for bacterial [4Fe-4S]-type ferredoxins. The residue that is four residues C-terminal to the fourth ligand of either cluster is generally an alanine or a cysteine. In five experimental ferredoxin structures, the cysteine has the same structural orientation rel...

journal_title：Biophysical journal

authors： Beck BW,Xie Q,Ichiye T

更新日期：2001-08-01 00:00:00

abstract：:Odorant-induced currents in mammalian olfactory receptor neurons have proved difficult to obtain reliably using conventional whole-cell recording. By using a mathematical model of the electrical circuit of the patch and rest-of-cell, we demonstrate how cell-attached patch measurements can be used to quantitatively ana...

journal_title：Biophysical journal

authors： Chiu P,Lynch JW,Barry PH

更新日期：1997-03-01 00:00:00

abstract：:Electron cryomicroscopy of rotor complexes of the Salmonella typhimurium flagellar motor, overproduced in a nonmotile Escherichia coli host, has revealed a variation in subunit symmetry of the cytoplasmic ring (C ring) module. C rings with subunit symmetries ranging from 31 to 38 were found. They formed a Gaussian dis...

journal_title：Biophysical journal

authors： Young HS,Dang H,Lai Y,DeRosier DJ,Khan S

更新日期：2003-01-01 00:00:00