Nonlinear force-length relationship in the ADP-induced contraction of skeletal myofibrils.

abstract：:Juxtacrine signaling is an important class of signaling systems that plays a crucial role in various developmental processes ranging from coordination of differentiation between neighboring cells to guiding axon growth during neurogenesis. Such signaling systems rely on the interaction between receptors on one cell an...

journal_title：Biophysical journal

authors： Yaron T,Cordova Y,Sprinzak D

更新日期：2014-11-18 00:00:00

abstract：:Cholesterol, due to its condensing effect, is considered an important regulator of membrane thickness. Other sterols, due to their structural similarities to cholesterol, are often assumed to have a universal effect on membrane properties similar to the condensing effect of cholesterol, albeit possibly to different de...

journal_title：Biophysical journal

authors： Hung WC,Lee MT,Chung H,Sun YT,Chen H,Charron NE,Huang HW

更新日期：2016-05-10 00:00:00

abstract：:Model compound studies in the literature show how Hofmeister ion interactions affect protein stability. Although model compound results are typically obtained as salting-out constants, they can be used to find out how the interactions affect protein stability. The null point in the Hofmeister series, which divides pro...

journal_title：Biophysical journal

authors： Baldwin RL

更新日期：1996-10-01 00:00:00

abstract：:In recent in vitro experiments, it has been demonstrated that the 47-kDa fragment of the talin molecule and the 32-kDa fragment of the vinculin molecule interact with acidic phospholipids. By using a computer analysis method, we determined the hydrophobic and amphipathic stretches of these fragments and, by applying a...

journal_title：Biophysical journal

authors： Tempel M,Goldmann WH,Isenberg G,Sackmann E

更新日期：1995-07-01 00:00:00

abstract：:The availability of the structures of the cytochrome b6f complex (cyt b6f), plastocyanin (PC), and cytochrome c6 (cyt c6) from Chlamydomonas reinhardtii allowed us, for the first time, to model electron transfer interactions between the luminal domains of this complex (including cyt f and the Rieske FeS protein) and i...

journal_title：Biophysical journal

authors： Haddadian EJ,Gross EL

更新日期：2006-10-01 00:00:00

abstract：:The theoretical basis of an optical microscope technique to image dynamically scattered light fluctuation decay rates (dynamic light scattering microscopy) is developed. It is shown that relative motions between scattering centers even smaller than the optical resolution of the microscope are sufficient to produce sig...

journal_title：Biophysical journal

authors： Dzakpasu R,Axelrod D

更新日期：2004-08-01 00:00:00

abstract：:Rapid and robust methods are required to quantify the effect of hydrodynamic shear on protein conformation change. We evaluated such strategies in this work and found that the binding of the fluorescent probe 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid (bis-ANS) to hydrophobic pockets in the blood protein von ...

journal_title：Biophysical journal

authors： Themistou E,Singh I,Shang C,Balu-Iyer SV,Alexandridis P,Neelamegham S

更新日期：2009-11-04 00:00:00

abstract：:Two independent methods, induced osmosis and solvent drag, were used to determine the reflection coefficients for NaCl (sigma NaCl) in brush border and basolateral membrane vesicles isolated from rabbit proximal tubule. In the induced osmosis method, vesicles loaded with sucrose were subjected to varying inward NaCl g...

journal_title：Biophysical journal

authors： Pearce D,Verkman AS

更新日期：1989-06-01 00:00:00

abstract：:Actin filaments are semiflexible polymers that display large-scale conformational twisting and bending motions. Modulation of filament bending and twisting dynamics has been linked to regulatory actin-binding protein function, filament assembly and fragmentation, and overall cell motility. The relationship between act...

journal_title：Biophysical journal

authors： De La Cruz EM,Roland J,McCullough BR,Blanchoin L,Martiel JL

更新日期：2010-09-22 00:00:00

abstract：:The shapes of fluorescence induction curves in spinach chloroplasts, measured using double-flash pump-probe techniques, are shown to depend on the duration of the actinic flashes. For flash durations tau(0)

journal_title：Biophysical journal

authors： Valkunas L,Geacintov NE,France L,Breton J

更新日期：1991-02-01 00:00:00

abstract：:Phospholipids with covalently attached poly(ethylene glycol) (PEG lipids) are commonly used for the preparation of long circulating liposomes. Although it is well known that lipid/PEG-lipid mixed micelles may form above a certain critical concentration of PEG-lipid, little is known about the effects of PEG-lipids on l...

journal_title：Biophysical journal

authors： Edwards K,Johnsson M,Karlsson G,Silvander M

更新日期：1997-07-01 00:00:00

abstract：:Fibrin polymerizes to produce branching fibers forming a three-dimensional network, which has been difficult to visualize by conventional microscopy. Three-dimensional images of whole clots at high resolution were obtained from stereo-pair intermediate-voltage electron micrographs. Computer software was developed to p...

journal_title：Biophysical journal

authors： Baradet TC,Haselgrove JC,Weisel JW

更新日期：1995-04-01 00:00:00

abstract：:Although the majority of free cellular cholesterol is present in the plasma membrane, cholesterol homeostasis is principally regulated through sterol-sensing proteins that reside in the cholesterol-poor endoplasmic reticulum (ER). In response to acute cholesterol loading or depletion, there is rapid equilibration betw...

journal_title：Biophysical journal

authors： Olsen BN,Bielska AA,Lee T,Daily MD,Covey DF,Schlesinger PH,Baker NA,Ory DS

更新日期：2013-10-15 00:00:00

abstract：:Communication between cells is crucial for proper functioning of multicellular organisms. The recently discovered membranous tubes, named tunneling nanotubes, that directly bridge neighboring cells may offer a very specific and effective way of intercellular communication. Our experiments on RT4 and T24 urothelial cel...

journal_title：Biophysical journal

authors： Veranic P,Lokar M,Schütz GJ,Weghuber J,Wieser S,Hägerstrand H,Kralj-Iglic V,Iglic A

更新日期：2008-11-01 00:00:00

abstract：:Steady-state polarization-resolved fluorescence imaging is used to analyze the molecular orientational order behavior of rigidly labeled major histocompatibility complex class I (MHC I) proteins and lipid probes in cell membranes of living cells. These fluorescent probes report the orientational properties of proteins...

journal_title：Biophysical journal

authors： Kress A,Ferrand P,Rigneault H,Trombik T,He HT,Marguet D,Brasselet S

更新日期：2011-07-20 00:00:00

abstract：:The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucial role in platelet aggregation and hemostasis. We have developed a model system based on laser tweezers, enabling us to measure specific rupture forces needed to separate single receptor-ligand complexes. First of all, we performed a thor...

journal_title：Biophysical journal

authors： Litvinov RI,Bennett JS,Weisel JW,Shuman H

更新日期：2005-10-01 00:00:00

abstract：:We present the results of a detailed molecular dynamics study of the closed form of the P2X4 receptor. The fluctuations observed in the simulations were compared with the changes that occur in the transition from the closed to the open structure. To get further insight on the opening mechanism, the actual displacement...

journal_title：Biophysical journal

authors： Pierdominici-Sottile G,Moffatt L,Palma J

更新日期：2016-12-20 00:00:00

abstract：:The solubility of aqueous solutions of lysozyme in the presence of polyethylene glycol and various alkaline salts was studied experimentally. The protein-electrolyte mixture was titrated with polyethylene glycol, and when precipitation of the protein occurred, a strong increase of the absorbance at 340 nm was observed...

journal_title：Biophysical journal

authors： Boncina M,Rescic J,Vlachy V

更新日期：2008-08-01 00:00:00

abstract：:The polymorphic phase behavior of bovine heart cardiolipin (CL) in the presence of different divalent cations and the kinetics of CL vesicle fusion induced by these cations have been investigated. (31)P-NMR measurements of equilibrium cation-CL complexes showed the lamellar-to-hexagonal (L(alpha)-H(II)) transition tem...

journal_title：Biophysical journal

authors： Ortiz A,Killian JA,Verkleij AJ,Wilschut J

更新日期：1999-10-01 00:00:00

abstract：:We provide evidence that the onset of functional dynamics of folded proteins with elevated temperatures is associated with the effective sampling of its energy landscape under physiological conditions. The analysis is based on data describing the relaxation phenomena governing the backbone dynamics of bovine pancreati...

journal_title：Biophysical journal

authors： Baysal C,Atilgan AR

更新日期：2005-03-01 00:00:00

abstract：:We have previously observed that the hydrophobic polyelectrolyte poly(2-ethylacrylic acid) solubilizes lipid membranes in a pH-dependent manner, and we have exploited this phenomenon to prepare lipid vesicles that release their contents in response to pH, light, or glucose (Thomas, J. L., and D. A. Tirrell. Acc. Chem....

journal_title：Biophysical journal

authors： Thomas JL,Barton SW,Tirrell DA

更新日期：1994-09-01 00:00:00

abstract：:The structural basis of the wide variability of the physical properties of fibrin clots and the process of assembly of the clot were investigated by electron microscopy of fibers formed under various ionic conditions. In addition, highly specific proteolytic enzymes from different snake venoms were used to remove sele...

journal_title：Biophysical journal

authors： Weisel JW

更新日期：1986-12-01 00:00:00

abstract：:Nonconfluent fibroblasts are relatively depolarized when compared with confluent fibroblasts, and transient hyperpolarizations result from a range of external stimuli as well as internal cellular activities. This electrical activity ceases, along with growth and mitogenic activity, when the cells become confluent. A c...

journal_title：Biophysical journal

authors： Stockbridge LL,French AS

更新日期：1988-07-01 00:00:00

abstract：:The effect of cholesterol on phospholipid acyl chain packing in bilayers consisting of highly unsaturated acyl chains in the liquid crystalline phase was examined for a series of symmetrically and asymmetrically substituted phosphatidylcholines (PCs). The time-resolved fluorescence emission and decay of fluorescence a...

journal_title：Biophysical journal

authors： Mitchell DC,Litman BJ

更新日期：1998-08-01 00:00:00

abstract：:Molecular machines fueled by NTP play pivotal roles in a wide range of cellular activities. One common feature among NTP-driven molecular machines is that NTP binding is a major force-generating step among the elementary reaction steps comprising NTP hydrolysis. To understand the mechanism in detail,in this study, we ...

journal_title：Biophysical journal

authors： Arai HC,Yukawa A,Iwatate RJ,Kamiya M,Watanabe R,Urano Y,Noji H

更新日期：2014-07-01 00:00:00

abstract：:Two modes of behavior of single human red cells in a shear field have been described. It is known that in low viscosity media and at shear rates less than 20 s-1, the cells rotate with a periodically varying angular velocity, in accord with the theory of Jeffery (1922) for oblate spheroids. In media of viscosity great...

journal_title：Biophysical journal

authors： Bitbol M

更新日期：1986-05-01 00:00:00

abstract：:A new flow configuration allows a flow cytometer of high resolution and stability to be assembled from an inverted fluorescence microscope with incident illumination, a pulse photometer, and a multichannel pulse height analyzer. A nozzle produces a hydrodynamically focused sample stream in a liquid jet that id directe...

journal_title：Biophysical journal

authors： Lindmo T,Steen HB

更新日期：1979-10-01 00:00:00

abstract：:The reaction of c-cytochromes with iron hexacyanides is similar in mechanism to the interaction of cytochromes with their physiological oxidants and reductants in that the formation of complexes precedes electron transfer. Analysis of the kinetics of oxidation and reduction of a number of c-cytochromes by solving the ...

journal_title：Biophysical journal

authors： Ohno N,Cusanovich MA

更新日期：1981-12-01 00:00:00

abstract：:Fluo-3 is an unusual tetracarboxylate Ca2+ indicator. For recent lots supplied by Molecular Probes Inc. (Eugene, OR), FMAX, the fluorescence intensity of the indicator in its Ca(2+)-bound form, is approximately 200 times that of FMIN, the fluorescence intensity of the indicator in its Ca(2+)-free form. (For earlier lo...

journal_title：Biophysical journal

authors： Harkins AB,Kurebayashi N,Baylor SM

更新日期：1993-08-01 00:00:00

abstract：:Heterogeneity of ADP diffusion and regulation of respiration were studied in permeabilized cardiomyocytes and cardiac fibers in situ and in silico. Regular arrangement of mitochondria in cells was altered by short-time treatment with trypsin and visualized by confocal microscopy. Manipulation of matrix volumes by chan...

journal_title：Biophysical journal

authors： Saks V,Kuznetsov A,Andrienko T,Usson Y,Appaix F,Guerrero K,Kaambre T,Sikk P,Lemba M,Vendelin M

更新日期：2003-05-01 00:00:00