Effect of polyethyleneglycol-phospholipids on aggregate structure in preparations of small unilamellar liposomes.

abstract：:Recent experimental studies of the role of microtubule sliding in neurite outgrowth suggested a qualitative model, according to which kinesin-1 motors push the minus-end-out microtubules against the cell membrane and generate the early cell processes. At the later stage, dynein takes over the sliding, expels the minus...

journal_title：Biophysical journal

authors： Oelz DB,Del Castillo U,Gelfand VI,Mogilner A

更新日期：2018-10-16 00:00:00

abstract：:The mathematics of the binomial model for quantal neurotransmitter release is considered in general terms, to explore what information might be extractable from statistical aspects of data. For an array of N statistically independent release sites, each with a release probability p, the compound binomial always pertai...

journal_title：Biophysical journal

authors： Quastel DM

更新日期：1997-02-01 00:00:00

abstract：:Crystals have been grown of "sperm whale" myoglobin produced in Escherichia coli from a synthetic gene and the structure has been solved to 1.9 A resolution. Because of a remaining initiator methionine, this protein crystallizes in a different space group from native sperm whale myoglobin. The three-dimensional struct...

journal_title：Biophysical journal

authors： Phillips GN Jr

更新日期：1990-02-01 00:00:00

abstract：:An algorithm needed for computer simulation of immunodiffusion has been deduced from existing theories of the in vitro reaction between antibody and antigen. The "Goldberg most probable polymer distribution" theory provides a formula that gives the amount of free antibody, free antigen, and diffusible complexes from e...

journal_title：Biophysical journal

authors： Trautman R

更新日期：1973-05-01 00:00:00

abstract：:The determination of intracellular protein concentrations is a prerequisite for understanding protein interaction networks in systems biology. Today, protein quantification is based either on mass spectrometry, which requires large cell numbers and sophisticated measurement protocols, or on quantitative Western blotti...

journal_title：Biophysical journal

authors： Chung YD,Sinzinger MD,Bovee-Geurts P,Krause M,Dinkla S,Joosten I,Koopman WJ,Adjobo-Hermans MJ,Brock R

更新日期：2011-12-07 00:00:00

abstract：:We present a theoretical model for aqueous solutions of double-stranded (ds) DNA with explicit consideration of electrostatic interactions, excluded-volume effects, van der Waals attractions, and salt ions. With reasonable parameters estimated from the DNA structure and experimental data for electrolytes, we are able ...

journal_title：Biophysical journal

authors： Li Z,Wu J,Wang ZG

更新日期：2008-02-01 00:00:00

abstract：:Observation of cell membrane buckling and cell folding in micropipette aspiration experiments was used to evaluate the bending rigidity of the red blood cell membrane. The suction pressure required to buckle the membrane surface initially was found to be about one-half to two-thirds of the pressure that caused the cel...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1983-07-01 00:00:00

abstract：:The stoichiometric matrix, S, represents a mapping of reaction rate vectors into a space of concentration time derivatives. The left null space of the stoichiometric matrix contains the dynamic invariants: a combination of concentration variables, referred to as metabolic pools, whose total concentration does not chan...

journal_title：Biophysical journal

authors： Famili I,Palsson BO

更新日期：2003-07-01 00:00:00

abstract：:Keratin intermediate filaments form dynamic intracellular networks, which span the entire cytoplasm and provide mechanical strength to the cell. The mechanical resilience of the keratin intermediate filament network itself is determined by filament bundling. The bundling process can be reproduced in artificial conditi...

journal_title：Biophysical journal

authors： Haimov E,Windoffer R,Leube RE,Urbakh M,Kozlov MM

更新日期：2020-07-07 00:00:00

abstract：:Our ability to understand speech requires neural tuning with high frequency resolution, but the peripheral mechanisms underlying sharp tuning in humans remain unclear. Sharp tuning in genetically modified mice has been attributed to decreases in spread of excitation of tectorial membrane traveling waves. Here we show ...

journal_title：Biophysical journal

authors： Farrahi S,Ghaffari R,Sellon JB,Nakajima HH,Freeman DM

更新日期：2016-09-06 00:00:00

abstract：:The closed-state crystal structure of prokaryotic inward rectifier, KirBac1.1, has implicated four inner helical phenylalanines near the cytoplasmic side as a possible locus of the channel gate. In the present study, we investigate whether this structural feature corresponds to the physiological pH gate of the renal i...

journal_title：Biophysical journal

authors： Sackin H,Nanazashvili M,Palmer LG,Krambis M,Walters DE

更新日期：2005-04-01 00:00:00

abstract：:We showed recently that the high-salt transition of poly[d(G-C)]. poly[d(G-C)] between B-DNA and Z-DNA (at [NaCl] = 2.25 M or [MgCl(2)] = 0.7 M) can be ascribed to the lesser electrostatic free energy of the B form, due to better immersion of the phosphates in the solution. This property was incorporated in cylindrica...

journal_title：Biophysical journal

authors： Guéron M,Demaret J,Filoche M

更新日期：2000-02-01 00:00:00

abstract：:Native cGMP-gated channels were studied in rod outer segments of the larval tiger salamander Ambystoma tigrinum. The alpha subunit of the cGMP-gated channel from bovine rods, here referred to as the wild type (w.t.), and mutant channels were heterologously expressed in Xenopus laevis oocytes. These channels were studi...

journal_title：Biophysical journal

authors： Sesti F,Nizzari M,Torre V

更新日期：1996-06-01 00:00:00

abstract：:Molecular chaperones are large proteins or protein complexes from which many proteins require assistance in order to fold. One unique property of molecular chaperones is the cavity they provide in which proteins fold. The interior surface residues which make up the cavities of molecular chaperone complexes from differ...

journal_title：Biophysical journal

authors： White AD,Huang W,Jiang S

更新日期：2012-12-19 00:00:00

abstract：:A new solid-state NMR-based strategy is established for the precise and efficient analysis of orientation and dynamics of transmembrane peptides in fluid bilayers. For this purpose, several dynamically averaged anisotropic constraints, including (13)C and (15)N chemical shift anisotropies and (13)C-(15)N dipolar coupl...

journal_title：Biophysical journal

authors： Holt A,Rougier L,Réat V,Jolibois F,Saurel O,Czaplicki J,Killian JA,Milon A

更新日期：2010-05-19 00:00:00

abstract：:Dimerization of the transmembrane (TM) adaptor protein DAP12 plays a key role in mediating activation signals through TM-TM association with cell-surface receptors. Herein, we apply the TOXCAT assay and molecular dynamics simulation to analyze dynamics and dimerization of the TM helix of DAP12 in the membrane bilayer....

journal_title：Biophysical journal

authors： Wei P,Zheng BK,Guo PR,Kawakami T,Luo SZ

更新日期：2013-04-02 00:00:00

abstract：:The chemical unfolding transition of a protein was simulated, including the presence of an intermediate (I) in equilibrium with the native (N) and unfolded (U) states. The calculations included free energies of unfolding, DeltaGuw, in the range of 1.4 kcal/mol to 10 kcal/mol and three different global m-values. The si...

journal_title：Biophysical journal

authors： Soulages JL

更新日期：1998-07-01 00:00:00

abstract：:Osmotic stress in the 0.5-5 x 10(6) dyne/cm2 range was used to perturb the hydration of actin-myosin-ATP intermediates during steady-state hydrolysis. Polyethylene glycol (PEG) (1000 to 4000 Da), in the 1 to 10 wt% range, which does not cause protein precipitation, did not significantly affect the apparent KM or the V...

journal_title：Biophysical journal

authors： Highsmith S,Duignan K,Cooke R,Cohen J

更新日期：1996-06-01 00:00:00

abstract：:The general formalism required to treat two-state sliding filament models of muscle contraction, including free energy considerations, is first reviewed and amplified. This formalism is then used to examine, and modify as needed, three models studied previously by Podolsky and Nolan, in which cross-bridge attachment-d...

journal_title：Biophysical journal

authors： Hill TL,Eisenberg E,Chen YD,Podolsky RJ

更新日期：1975-04-01 00:00:00

abstract：:The endoplasmic reticulum (ER) is the major compartment for the processing and quality control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a noninvasive probe to determine the viscous properties of the aqueous lumen of the ER. GFP was targeted to the ER lumen of CHO cells by transient tr...

journal_title：Biophysical journal

authors： Dayel MJ,Hom EF,Verkman AS

更新日期：1999-05-01 00:00:00

abstract：:In an attempt to quantitate the physical behavior of biological systems, Fourier analysis has been applied to the respiratory and circulatory systems by a number of investigators. The validity of this application has been questioned on the basis that these systems are nonlinear and not strictly periodic. If these obje...

journal_title：Biophysical journal

authors： Attinger EO,Anné A,McDonald DA

更新日期：1966-05-01 00:00:00

abstract：:Secondary structure formation of nucleic acids strongly depends on salt concentration and temperature. We develop a theory for RNA folding that correctly accounts for sequence effects, the entropic contributions associated with loop formation, and salt effects. Using an iterative expression for the partition function ...

journal_title：Biophysical journal

authors： Einert TR,Netz RR

更新日期：2011-06-08 00:00:00

abstract：:Adsorption-desorption isotherms were obtained for water binding by 1,2-dimyristoylphosphatidylcholine in the temperature range 15 degrees-35 degrees C. The isotherms were analyzed by Brunauer et al.'s (BET) theory and also a polarization theory, the latter being more successful in fitting the data. There was some evid...

journal_title：Biophysical journal

authors： Wilkinson DA,Morowitz HJ,Prestegard JH

更新日期：1977-11-01 00:00:00

abstract：:In this article, we explore the information content of molecular force-field calculations. We make use of exhaustive lattice models of molecular conformations and reduced alphabet sequences to determine the relative resolving power of pairwise interaction-based force fields. We find that sequence-specific interactions...

journal_title：Biophysical journal

authors： Aynechi T,Kuntz ID

更新日期：2005-11-01 00:00:00

abstract：:Water plays an important role in enzyme structure and function in aqueous media. That role becomes even more important when one focuses on enzymes in low water media. Here we present results from molecular dynamics simulations of surfactant-solubilized subtilisin BPN' in three organic solvents (octane, tetrahydrofuran...

journal_title：Biophysical journal

authors： Yang L,Dordick JS,Garde S

更新日期：2004-08-01 00:00:00

abstract：:Förster resonance energy transfer (FRET)-based biosensors for the quantitative analysis of intracellular signaling, including sensors for monitoring cyclic adenosine monophosphate (cAMP), are of increasing interest. The measurement of the donor/acceptor emission ratio in tandem biosensors excited at the donor excitati...

journal_title：Biophysical journal

authors： Salonikidis PS,Zeug A,Kobe F,Ponimaskin E,Richter DW

更新日期：2008-12-01 00:00:00

abstract：:Large-conductance Ca(2+)-activated K(+) channels (BK) play a fundamental role in modulating membrane potential in many cell types. The gating of BK channels and its modulation by Ca(2+) and voltage has been the subject of intensive research over almost three decades, yielding several of the most complicated kinetic me...

journal_title：Biophysical journal

authors： Rosales RA,Varanda WA

更新日期：2009-05-20 00:00:00

abstract：:The surprising observation that pressures of the order of 150 atmospheres can restore consciousness to an anesthetized animal has long been central to theories of the molecular mechanisms underlying general anesthesia. We have constructed a high-pressure gas chamber to test for "pressure reversal" of the best availabl...

journal_title：Biophysical journal

authors： Moss GW,Lieb WR,Franks NP

更新日期：1991-12-01 00:00:00

abstract：:Communication between cells is crucial for proper functioning of multicellular organisms. The recently discovered membranous tubes, named tunneling nanotubes, that directly bridge neighboring cells may offer a very specific and effective way of intercellular communication. Our experiments on RT4 and T24 urothelial cel...

journal_title：Biophysical journal

authors： Veranic P,Lokar M,Schütz GJ,Weghuber J,Wieser S,Hägerstrand H,Kralj-Iglic V,Iglic A

更新日期：2008-11-01 00:00:00

abstract：:A mathematical model is formulated for the development of a population of cells in which the individual members may grow and divide or die. A given cell is characterized by its age and volume, and these parameters are assumed to determine the rate of volume growth and the probability per unit time of division or death...

journal_title：Biophysical journal

authors： Bell GI,Anderson EC

更新日期：1967-07-01 00:00:00