Osmotic pressure and packaging structure of caged DNA.

abstract：:The amyloid fibrillar form of the protein Tau is involved in a number of neurodegenerative diseases, also known as tauopathies. In this work, six different fibrillar Tau isoforms were assembled in vitro. The morphological and nanomechanical properties of these isoforms were studied using atomic force microscopy at hig...

journal_title：Biophysical journal

authors： Makky A,Bousset L,Madiona K,Melki R

更新日期：2020-12-15 00:00:00

abstract：:The mechanisms by which extracellular electric field stimuli induce the (re)excitation of cardiac cells in various stages of refractoriness are still not well understood. We modeled the interactions between an isolated cardiac cell and imposed extracellular electric fields to determine the mechanisms by which relative...

journal_title：Biophysical journal

authors： Fishler MG,Sobie EA,Thakor NV,Tung L

更新日期：1996-03-01 00:00:00

abstract：:Singlet-singlet annihilation experiments have been performed on trimeric and aggregated light-harvesting complex II (LHCII) using picosecond spectroscopy to study spatial equilibration times in LHCII preparations, complementing the large amount of data on spectral equilibration available in literature. The annihilatio...

journal_title：Biophysical journal

authors： Barzda V,Gulbinas V,Kananavicius R,Cervinskas V,van Amerongen H,van Grondelle R,Valkunas L

更新日期：2001-05-01 00:00:00

abstract：:Myosin II is a central mechanoenzyme in a wide range of cellular morphogenic processes. Its cellular localization is dependent not only on signal transduction pathways, but also on mechanical stress. We suggest that this stress-dependent distribution is the result of both the force-dependent binding to actin filaments...

journal_title：Biophysical journal

authors： Luo T,Mohan K,Srivastava V,Ren Y,Iglesias PA,Robinson DN

更新日期：2012-01-18 00:00:00

abstract：:GABA and glycine receptors (GlyRs) are pentameric ligand-gated ion channels that respond to the inhibitory neurotransmitters by opening a chloride-selective central pore lined with five M2 segments homologous to those of alpha(1) GlyR/ ARVG(2')LGIT(6')TVLTMTTQSSGSR. The activity of cyanotriphenylborate (CTB) and picro...

journal_title：Biophysical journal

authors： Zhorov BS,Bregestovski PD

更新日期：2000-04-01 00:00:00

abstract：:Saturation transfer electron paramagnetic resonance (ST-EPR) spectroscopy has been employed to characterize the very slow microsecond to millisecond rotational dynamics of a wide range of nitroxide spin-labeled proteins and other macromolecules in the past three decades. The vast majority of this previous work has bee...

journal_title：Biophysical journal

authors： Hustedt EJ,Beth AH

更新日期：2004-06-01 00:00:00

abstract：:This study aims to define the role of E-cadherin (Ecad) engagement in cell-cell contact during membrane-cortex interaction. As a tool, we used a hydrodynamic membrane tube extrusion technique to characterize the mechanical interaction between the plasma membrane and the underlying cortical cytoskeleton. Cells were anc...

journal_title：Biophysical journal

authors： Tabdanov E,Borghi N,Brochard-Wyart F,Dufour S,Thiery JP

更新日期：2009-03-18 00:00:00

abstract：:Dynamic properties of class A beta-lactamase TEM-1 are investigated from molecular dynamics (MD) simulations. Comparison of MD-derived order parameters with those obtained from model-free analysis of nuclear magnetic resonance (NMR) relaxation data shows high agreement for N-H moieties within alpha- and beta-secondary...

journal_title：Biophysical journal

authors： Fisette O,Morin S,Savard PY,Lagüe P,Gagné SM

更新日期：2010-02-17 00:00:00

abstract：:We study theoretically the feasibility of using transverse electronic transport within a nanopore for rapid DNA sequencing. Specifically, we examine the effects of the environment and detection probes on the distinguishability of the DNA bases. We find that the intrinsic measurement bandwidth of the electrodes helps t...

journal_title：Biophysical journal

authors： Lagerqvist J,Zwolak M,Di Ventra M

更新日期：2007-10-01 00:00:00

abstract：:Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human...

journal_title：Biophysical journal

authors： Diop M,Taylor R

更新日期：2006-05-15 00:00:00

abstract：:Juxtacrine signaling is an important class of signaling systems that plays a crucial role in various developmental processes ranging from coordination of differentiation between neighboring cells to guiding axon growth during neurogenesis. Such signaling systems rely on the interaction between receptors on one cell an...

journal_title：Biophysical journal

authors： Yaron T,Cordova Y,Sprinzak D

更新日期：2014-11-18 00:00:00

abstract：:Emission spectra of the photolabile Ca2+ chelators DM-nitrophen, nitr-5, and diazo-2 were studied alone, and in the presence of indo-1, to investigate potential interactions that would make the simultaneous manipulation and ratiometric measurement of the intracellular Ca2+ concentration difficult. Neither diazo-2 nor ...

journal_title：Biophysical journal

authors： Hadley RW,Kirby MS,Lederer WJ,Kao JP

更新日期：1993-12-01 00:00:00

abstract：:Hemolymph and protein-free hemolymph obtained from Loligo pealei were analyzed for cation concentration by the method of atomic absorption spectroscopy, for the following ions: Na+, K+, Ca++, and Mg++. No significant differences were seen in ion concentration between hemolymph and protein-free hemolymph. Of particular...

journal_title：Biophysical journal

authors： Shoukimas J,Adelman WJ Jr,Sege V

更新日期：1977-05-01 00:00:00

abstract：:The contribution of a specific hydrogen bond in apoflavodoxin to protein stability is investigated by combining theory, experiment and simulation. Although hydrogen bonds are major determinants of protein structure and function, their contribution to protein stability is still unclear and widely debated. The best meth...

journal_title：Biophysical journal

authors： Campos LA,Cuesta-López S,López-Llano J,Falo F,Sancho J

更新日期：2005-02-01 00:00:00

abstract：:We investigate a temperature-driven recursive division of binary giant unilamellar vesicles (GUVs). During the heating step of the heating-cooling cycle, the spherical mother vesicle deforms to a budded limiting shape using up the excess area produced by the chain melting of the lipids and then splits off into two dau...

journal_title：Biophysical journal

authors： Jimbo T,Sakuma Y,Urakami N,Ziherl P,Imai M

更新日期：2016-04-12 00:00:00

abstract：:The mechanisms by which the mammalian mitotic spindle is guided to a predefined orientation through microtubule-cortex interactions have recently received considerable interest, but there has been no dynamic model that describes spindle movements toward the preferred axis in human cells. Here, we develop a dynamic mod...

journal_title：Biophysical journal

authors： Corrigan AM,Shrestha R,Draviam VM,Donald AM

更新日期：2015-10-06 00:00:00

abstract：:39K nuclear magnetic resonance (NMR) spectra were readily obtained, in vivo, from rat muscle, kidney, and brain in 5-10 min with signal-to-noise ratios of approximately 20:1. Quantitation of the K+ signal was achieved by reference to an external standard of KCl/dysprosium nitrate as well as by reference to the proton ...

journal_title：Biophysical journal

authors： Adam WR,Koretsky AP,Weiner MW

更新日期：1987-02-01 00:00:00

abstract：:The channel-forming properties of two analogs of gramicidin, gramicidin-ethylenediamine (gram-EDA), and gramicidin-N,N-dimethylethylenediamine (gram-DMEDA) were studied in planar lipid bilayers, using protons as the permeant ion. These peptides have positively charged amino groups tethered to their C-terminal ends via...

journal_title：Biophysical journal

authors： Woolley GA,Zunic V,Karanicolas J,Jaikaran AS,Starostin AV

更新日期：1997-11-01 00:00:00

abstract：:A nitroxide spin label attached to the C-terminus of the channel forming peptide alamethicin produces an enhancement of the nuclear spin-lattice relaxation rates of peptide protons as a result of both intermolecular and intramolecular magnetic dipole-dipole interactions. The intermolecular contribution provides eviden...

journal_title：Biophysical journal

authors： North CL,Franklin JC,Bryant RG,Cafiso DS

更新日期：1994-11-01 00:00:00

abstract：:Protein conformational fluctuations modulate the catalytic powers of enzymes. The frequency of conformational fluctuations may modulate the catalytic rate at individual reaction steps. In this study, we modulated the rotary fluctuation frequency of F1-ATPase (F1) by attaching probes with different viscous drag coeffic...

journal_title：Biophysical journal

authors： Watanabe R,Hayashi K,Ueno H,Noji H

更新日期：2013-11-19 00:00:00

abstract：:In the photoactive yellow protein, PYP, both Glu46 and Tyr42 form hydrogen bonds to the phenolic OH group of the p-hydroxycinnamoyl chromophore. Previous work on replacement of the carboxyl group of Glu46 by an amide group (Glu46Gln) has shown that changing the nature of this hydrogen bond has a minimal effect on the ...

journal_title：Biophysical journal

authors： Devanathan S,Lin S,Cusanovich MA,Woodbury N,Tollin G

更新日期：2001-10-01 00:00:00

abstract：:A detailed experimental description of molecular dynamics requires an accurate description of the global correlation time. For the gramicidin cation-selective channel the local dynamics of the polypeptide backbone are thought to play a very significant role in the functional process of this channel which occurs on the...

journal_title：Biophysical journal

authors： Lee KC,Hu W,Cross TA

更新日期：1993-09-01 00:00:00

abstract：:Femtosecond time-resolved transient absorption spectroscopy was employed to characterize for the first time the primary photoisomerization dynamics of a bacterial phytochrome system in the two thermally stable states of the photocycle. The 85-kDa phytochrome Cph1 from the cyanobacterium Synechocystis PCC 6803 expresse...

journal_title：Biophysical journal

authors： Heyne K,Herbst J,Stehlik D,Esteban B,Lamparter T,Hughes J,Diller R

更新日期：2002-02-01 00:00:00

abstract：:The nucleus has a smooth, regular appearance in normal cells, and its shape is greatly altered in human pathologies. Yet, how the cell establishes nuclear shape is not well understood. We imaged the dynamics of nuclear shaping in NIH3T3 fibroblasts. Nuclei translated toward the substratum and began flattening during t...

journal_title：Biophysical journal

authors： Li Y,Lovett D,Zhang Q,Neelam S,Kuchibhotla RA,Zhu R,Gundersen GG,Lele TP,Dickinson RB

更新日期：2015-08-18 00:00:00

abstract：:Although both RNA and proteins have densely packed native structures, chain organizations of these two biopolymers are fundamentally different. Motivated by the recent discoveries in chromatin folding that interphase chromosomes have territorial organization with signatures pointing to metastability, we analyzed the b...

journal_title：Biophysical journal

authors： Liu L,Hyeon C

更新日期：2016-06-07 00:00:00

abstract：:The photoreceptor rhodopsin is a G-protein coupled receptor that has recently been proposed to exist as a dimer or higher order oligomer, in contrast to the previously described monomer, in retinal rod outer segment disk membranes. Rhodopsin exhibits considerably greater thermal stability than opsin (the bleached form...

journal_title：Biophysical journal

authors： Edrington TC 5th,Bennett M,Albert AD

更新日期：2008-09-15 00:00:00

abstract：:Single-particle tracking is an important technique in the life sciences to understand the kinetics of biomolecules. The analysis of apparent diffusion coefficients in vivo, for example, enables researchers to determine whether biomolecules are moving alone, as part of a larger complex, or are bound to large cellular c...

journal_title：Biophysical journal

authors： Vink JNA,Brouns SJJ,Hohlbein J

更新日期：2020-11-17 00:00:00

abstract：:Tip links are thought to gate the mechanically sensitive transduction channels of hair cells, but how they form during development and regeneration remains mysterious. In particular, it is unclear how tip links are strung between stereocilia so that they are oriented parallel to a single axis; why their polarity is un...

journal_title：Biophysical journal

authors： Tompkins N,Spinelli KJ,Choi D,Barr-Gillespie PG

更新日期：2017-10-17 00:00:00

abstract：:Electric field pulses have been reported to induce long-lived permeabilization and fusogenicity on cell membranes. The two membrane property alterations are under the control of the field strength, the pulse duration, and the number of pulses. Experiments on mammalian cells pulsed by square wave form pulses and then b...

journal_title：Biophysical journal

authors： Teissié J,Ramos C

更新日期：1998-04-01 00:00:00

abstract：:Members of the epidermal growth factor receptor family play important roles in various cellular processes, both in physiological and in pathological conditions. Dimerization and autophosphorylation of these receptor tyrosine kinases are key events of signal transduction. Details of the molecular events of the signalin...

journal_title：Biophysical journal

authors： Bagossi P,Horváth G,Vereb G,Szöllösi J,Tözsér J

更新日期：2005-02-01 00:00:00