Correlation between electric field pulse induced long-lived permeabilization and fusogenicity in cell membranes.

abstract：:Globotriaosylceramide (Gb3), a glycosphingolipid found in the plasma membrane of animal cells, is the endocytic receptor of the bacterial Shiga toxin. Using x-ray reflectivity (XR) and grazing incidence x-ray diffraction (GIXD), lipid monolayers containing Gb3 were investigated at the air-water interface. XR probed Gb...

journal_title：Biophysical journal

authors： Watkins EB,Gao H,Dennison AJC,Chopin N,Struth B,Arnold T,Florent JC,Johannes L

更新日期：2014-09-02 00:00:00

abstract：:Emission spectra of the photolabile Ca2+ chelators DM-nitrophen, nitr-5, and diazo-2 were studied alone, and in the presence of indo-1, to investigate potential interactions that would make the simultaneous manipulation and ratiometric measurement of the intracellular Ca2+ concentration difficult. Neither diazo-2 nor ...

journal_title：Biophysical journal

authors： Hadley RW,Kirby MS,Lederer WJ,Kao JP

更新日期：1993-12-01 00:00:00

abstract：:The classical cable equation, in which membrane conductance is considered constant, is modified by including the linearized effect of membrane potential on sodium and potassium ionic currents, as formulated in the Hodgkin-Huxley equations for the squid giant axon. The resulting partial differential equation is solved ...

journal_title：Biophysical journal

authors： Sabah NH,Leibovic KN

更新日期：1969-10-01 00:00:00

abstract：:Cell motion is driven by interplay between the actin cytoskeleton and the cell adhesions in the front part of the cell. The actin network segregates into lamellipodium and lamellum, whereas the adhesion complexes are characteristically distributed underneath the actin system. Here, we suggest a computational model for...

journal_title：Biophysical journal

authors： Shemesh T,Bershadsky AD,Kozlov MM

更新日期：2012-04-18 00:00:00

abstract：:Air dried films of purple membranes (PM) from Halobacterium halobium containing the photochromic protein bacteriorhodopsin (BR) were prepared and the BR-photocycle of this material analyzed. The absorption maxima of the initial state B (lambda(max) = 570 nm) and the photochemical intermediate M (lambda(max) = 412 nm),...

journal_title：Biophysical journal

authors： Hampp N,Bräuchle C,Oesterhelt D

更新日期：1990-07-01 00:00:00

abstract：:The formation of linear protein fibrils has previously been shown to be enhanced by volume exclusion or crowding in the presence of a high concentration of chemically inert protein or polymer, and by adsorption to membrane surfaces. An equilibrium mesoscopic model for the combined effect of both crowding and adsorptio...

journal_title：Biophysical journal

authors： Hoppe T,Minton AP

更新日期：2015-02-17 00:00:00

abstract：:The dynamic packaging of DNA into chromatin is a key determinant of eukaryotic gene regulation and epigenetic inheritance. Nucleosomes are the basic unit of chromatin, and therefore the accessible states of the nucleosome must be the starting point for mechanistic models regarding these essential processes. Although t...

journal_title：Biophysical journal

authors： Schlingman DJ,Mack AH,Kamenetska M,Mochrie SGJ,Regan L

更新日期：2014-07-15 00:00:00

abstract：:Impact loading of articular cartilage causes extensive chondrocyte death. Cell membranes have a limited elastic range of 3-4% strain but are protected from direct stretch during physiological loading by their membrane reservoir, an intricate pattern of membrane folds. Using a finite-element model, we suggested previou...

journal_title：Biophysical journal

authors： Moo EK,Amrein M,Epstein M,Duvall M,Abu Osman NA,Pingguan-Murphy B,Herzog W

更新日期：2013-10-01 00:00:00

abstract：:Dipole moment, enthalpy, and entropy changes were calculated for hypothetical structural units which control the opening and closing of ionic channels in axon membranes. The changes of these thermodynamic functions were calculated both for activation (transition to intermediate complex) and for the structural transfor...

journal_title：Biophysical journal

authors： Levitan E,Palti Y

更新日期：1975-03-01 00:00:00

abstract：:A partially depolarized squid axon membrane is assumed to have a quasi-steady state negative resistance, the membrane potential is clamped at one point, and a distribution of potential along the axon is obtained from the cable equation. Nominal experimental values of -2 ohm cm(2) for the membrane and 6 ohm cm(2) for t...

journal_title：Biophysical journal

authors： COLE KS

更新日期：1961-05-01 00:00:00

abstract：:The impedance measured in a strip of heart tissue from the moth Hyalophora cecropia is fitted by circuit models of several configurations. The circuits include: (a) a single R-C circuit (b) a double R-C circuit (c) terminated transmission lines, and (d) a pattern of cells with cell-to-cell transmission paths. The last...

journal_title：Biophysical journal

authors： Stibitz GR,McCann FV

更新日期：1974-02-01 00:00:00

abstract：:Molecular dynamics simulations supplement single-molecule pulling experiments by providing the possibility of examining the full free energy landscape using many coordinates. Here, we use an all-atom structure-based model to study the force and temperature dependence of the unfolding of the protein filamin by applying...

journal_title：Biophysical journal

authors： Sun L,Noel JK,Sulkowska JI,Levine H,Onuchic JN

更新日期：2014-12-16 00:00:00

abstract：:Heterogeneity of ADP diffusion and regulation of respiration were studied in permeabilized cardiomyocytes and cardiac fibers in situ and in silico. Regular arrangement of mitochondria in cells was altered by short-time treatment with trypsin and visualized by confocal microscopy. Manipulation of matrix volumes by chan...

journal_title：Biophysical journal

authors： Saks V,Kuznetsov A,Andrienko T,Usson Y,Appaix F,Guerrero K,Kaambre T,Sikk P,Lemba M,Vendelin M

更新日期：2003-05-01 00:00:00

abstract：:This paper presents a study of the influence of normalization errors on size distributions obtained from the analysis of intensity fluctuations by photon correlation spectroscopy. The effects of these errors are demonstrated by means of computer-generated autocorrelation functions simulating light scattered from a mon...

journal_title：Biophysical journal

authors： Ruf H

更新日期：1989-07-01 00:00:00

abstract：:Chimeras consisting of the homologous skeletal dihydropyridine receptor (DHPR) beta1a subunit and the heterologous cardiac/brain beta2a subunit were used to determine which regions of beta1a were responsible for the skeletal-type excitation-contraction (EC) coupling phenotype. Chimeras were transiently transfected in ...

journal_title：Biophysical journal

authors： Sheridan DC,Cheng W,Carbonneau L,Ahern CA,Coronado R

更新日期：2004-08-01 00:00:00

abstract：:In the budding yeast nucleus, transfer RNA (tRNA) genes are considered to localize in the vicinity of the nucleolus; however, the use of Hi-C and fluorescent repressor-operator system techniques has clearly indicated that the tRNA genes are distributed not only around the nucleolus but also at other nuclear locations....

journal_title：Biophysical journal

authors： Tokuda N

更新日期：2020-05-05 00:00:00

abstract：:Gap junctions formed of connexin46 (Cx46) and connexin50 (Cx50) in lens fiber cells are crucial for maintaining lens transparency. We determined the functional properties of homotypic Cx46, heterotypic Cx46/Cx50, and heteromeric Cx46/Cx50 channels in a communication-deficient neuroblastoma (N2A) cell line, using dual ...

journal_title：Biophysical journal

authors： Hopperstad MG,Srinivas M,Spray DC

更新日期：2000-10-01 00:00:00

abstract：:A variety of amphiphilic helical peptides have been shown to exhibit a transition from adsorbing parallel to a membrane surface at low concentrations to inserting perpendicularly into the membrane at high concentrations. Furthermore, this transition has been correlated to the peptides' cytolytic activities. X-ray lame...

journal_title：Biophysical journal

authors： Wu Y,He K,Ludtke SJ,Huang HW

更新日期：1995-06-01 00:00:00

abstract：:Gangliosides are a class of glycosphingolipids (GSLs) with amphiphilic character that are found at the outer leaflet of the cell membranes, where their ability to organize into special domains makes them vital cell membrane components. However, a molecular understanding of GSL-rich membranes in terms of their clustere...

journal_title：Biophysical journal

authors： Patel DS,Park S,Wu EL,Yeom MS,Widmalm G,Klauda JB,Im W

更新日期：2016-11-01 00:00:00

abstract：:The addition of 10(-3) M p-chloromercuribenzene sulfonate (PCMBS) to the solution bathing the mucosal surface of rabbit colon has no effect on the rate of active Na transport but blocks or reverses the inhibitory action of amiloride. The tissue must be exposed to PCMBS for 20-30 min for a complete blocking effect, and...

journal_title：Biophysical journal

authors： Gottlieb GP,Turnheim K,Frizzell RA,Schultz SG

更新日期：1978-04-01 00:00:00

abstract：:In this paper we explore the properties of a mathematical model for the passive sodium permeability system of excitable membranes. This model is distinguished by the explicit inclusion of a rate constant which depends not on instantaneous voltage, but on rate of voltage change. Actually, the model is a rather modest m...

journal_title：Biophysical journal

authors： Jakobsson E,Scudiero C

更新日期：1975-06-01 00:00:00

abstract：:Voltage gated potassium channels open and inactivate in response to changes of the voltage across the membrane. After removal of the fast N-type inactivation, voltage gated Shaker K-channels (Shaker-IR) are still able to inactivate through a poorly understood closure of the ion conduction pore. This, usually slower, i...

journal_title：Biophysical journal

authors： Oliva C,González V,Naranjo D

更新日期：2005-08-01 00:00:00

abstract：:The proteorhodopsin family consists of retinal proteins of marine bacterial origin with optical properties adjusted to their local environments. For green proteorhodopsin, a highly specific mutation in the EF loop, A178R, has been found to cause a surprisingly large redshift of 20 nm despite its distance from the chro...

journal_title：Biophysical journal

authors： Mehler M,Scholz F,Ullrich SJ,Mao J,Braun M,Brown LJ,Brown RC,Fiedler SA,Becker-Baldus J,Wachtveitl J,Glaubitz C

更新日期：2013-07-16 00:00:00

abstract：:Small single-domain proteins often exhibit only a single free-energy barrier, or transition state, between the denatured and the native state. The folding kinetics of these proteins is usually explored via mutational analysis. A central question is which structural information on the transition state can be derived fr...

journal_title：Biophysical journal

authors： Weikl TR

更新日期：2008-02-01 00:00:00

abstract：:We have worked out a procedure for covalent binding of native biomacromolecules on flat gold surfaces for scanning probe microscopy in aqueous buffer solutions and for other nanotechnological applications, such as the direct measurement of interaction forces between immobilized macromolecules, of their elastomechanica...

journal_title：Biophysical journal

authors： Wagner P,Hegner M,Kernen P,Zaugg F,Semenza G

更新日期：1996-05-01 00:00:00

abstract：:This study presents a simplified version of the quasi-one-dimensional theory (Wu, J., E. A. Johnson, and J. M. Kootsey. 1996. A quasi-one-dimensional theory for anisotropic propagation of excitation in cardiac muscle. Biophys. J. 71:2427-2439) with two components of the extracellular current, along and perpendicular t...

journal_title：Biophysical journal

authors： Wu J,Wikswo JP Jr

更新日期：1997-11-01 00:00:00

abstract：:A Ca(2+) spark arises when a cluster of sarcoplasmic reticulum (SR) channels (ryanodine receptors or RyRs) opens to release calcium in a locally regenerative manner. Normally triggered by Ca(2+) influx across the sarcolemmal or transverse tubule membrane neighboring the cluster, the Ca(2+) spark has been shown to be t...

journal_title：Biophysical journal

authors： Sobie EA,Dilly KW,dos Santos Cruz J,Lederer WJ,Jafri MS

更新日期：2002-07-01 00:00:00

abstract：:The MreB actin-like cytoskeleton assembles into dynamic polymers that coordinate cell shape in many bacteria. In contrast to most other cytoskeleton systems, few MreB-interacting proteins have been well characterized. Here, we identify a small protein from Caulobacter crescentus, an assembly inhibitor of MreB (AimB). ...

journal_title：Biophysical journal

authors： Werner JN,Shi H,Hsin J,Huang KC,Gitai Z,Klein EA

更新日期：2020-08-04 00:00:00

abstract：:Transmembrane osmotic gradients applied on large unilamellar 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles were used to modulate the potency of melittin to induce leakage. Melittin, an amphipathic peptide, changes the permeability of vesicles, as studied using the release of entrapped calcein, a fluorescent marker...

journal_title：Biophysical journal

authors： Benachir T,Lafleur M

更新日期：1996-02-01 00:00:00

abstract：:The retinal Na+:Ca2+, K+exchanger cDNA was transiently expressed in human embryonic kidney (HEK 293) cells by transfection with plasmid DNA. The correct targeting of the expressed protein to the plasma membrane was confirmed by immunocytochemistry. The reverse exchange offrent (Ca2+ imported per Na+ extruded) was meas...

journal_title：Biophysical journal

authors： Navanglone A,Rispoli G,Gabellini N,Carafoli E

更新日期：1997-07-01 00:00:00