Voltage-dependent behavior of a "ball-and-chain" gramicidin channel.

abstract：:The insertion of myelin basic protein into microemulsion droplets of sodium bis (2-ethylhexyl) sulfosuccinate (AOT) has been studied by quasi-elastic light scattering. Measurements were made at both low and high molar ratios of water to surfactant, as a function of protein occupancy. The hydrodynamic radii of filled a...

journal_title：Biophysical journal

authors： Chatenay D,Urbach W,Cazabat AM,Vacher M,Waks M

更新日期：1985-12-01 00:00:00

abstract：:Although the structure, function, conformational dynamics, and controlled thermodynamics of proteins are manifested by their corresponding amino acid sequences, the natural rules for molecular design and their corresponding interplay remain obscure. In this study, we focused on the role of internal cavities of protein...

journal_title：Biophysical journal

authors： Maeno A,Sindhikara D,Hirata F,Otten R,Dahlquist FW,Yokoyama S,Akasaka K,Mulder FA,Kitahara R

更新日期：2015-01-06 00:00:00

abstract：:This paper describes a molecular dynamics and molecular mechanics study of the solvation and selectivity of the narrow pore and vestibule region of a model-built structure for the voltage-gated sodium channel. The particular structure used was one proposed by Guy and Durell. However, many of the features we saw would ...

journal_title：Biophysical journal

authors： Singh C,Sankararamakrishnan R,Subramaniam S,Jakobsson E

更新日期：1996-11-01 00:00:00

abstract：:The adhesion of giant unilamellar phospholipid vesicles to planar substrates coated with extracellular matrix mimetic cushions of hyaluronan is studied using quantitative reflection interference contrast microscopy. The absolute height of the vesicle membrane at the vicinity of the substrate is measured by considering...

journal_title：Biophysical journal

authors： Limozin L,Sengupta K

更新日期：2007-11-01 00:00:00

abstract：:In recent experimental reports, robust circadian oscillation of the phosphorylation level of KaiC has been reconstituted by incubating three cyanobacterial proteins, KaiA, KaiB, and KaiC, with ATP in vitro. This reconstitution indicates that protein-protein interactions and the associated ATP hydrolysis suffice to gen...

journal_title：Biophysical journal

authors： Nagai T,Terada TP,Sasai M

更新日期：2010-06-02 00:00:00

abstract：:In striated muscle thin filament activation is initiated by Ca(2+) binding to troponin C and augmented by strong myosin binding to actin (cross-bridge formation). Several lines of evidence have led us to hypothesize that thin filament properties may limit the level and rate of force development in cardiac muscle at al...

journal_title：Biophysical journal

authors： Regnier M,Martin H,Barsotti RJ,Rivera AJ,Martyn DA,Clemmens E

更新日期：2004-09-01 00:00:00

abstract：:Fitting dwell-time distributions with sums of exponentials is widely used to characterize histograms of open- and closed-interval durations recorded from single ion channels, as well as for other physical phenomena. However, it can be difficult to identify the contributing exponential components. Here we extend previo...

journal_title：Biophysical journal

authors： Landowne D,Yuan B,Magleby KL

更新日期：2013-06-04 00:00:00

abstract：:In total internal reflection fluorescence microscopy (TIRFM), fluorophores near a surface can be excited with evanescent waves, which decay exponentially with distance from the interface. Penetration depths of evanescent waves from 60 nm to 300 nm were generated by varying the angle of incidence of a laser beam. With ...

journal_title：Biophysical journal

authors： Rohrbach A

更新日期：2000-05-01 00:00:00

abstract：:A simple technique for forming "black" lipid bilayer membranes containing negligible amounts of alkyl solvent is described. The membranes are formed by the method of Mueller et al (Circulation. 1962. 26:1167.) from glyceryl monooleate (GMO) dispersed in squalene. The squalene forms an annulus to satisfy the boundary c...

journal_title：Biophysical journal

authors： White SH

更新日期：1978-09-01 00:00:00

abstract：:The proposition is made that the red cell membrane is a two-dimensional, incompressible material and a general stress-strain law is developed for finite deformations. In the linear form, the character of such a material is analogous to a two-dimensional Mooney material (e.g., rubber), indicating that the molecular str...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1973-09-01 00:00:00

abstract：:The rate of exchange of urea across the membranes of human erythrocytes (red blood cells) was quantified on the 1-s to 2-min timescale. (13)C-urea was hyperpolarized and subjected to rapid dissolution and the previously reported (partial) resolution of (13)C NMR resonances from the molecules inside and outside red blo...

journal_title：Biophysical journal

authors： Pagès G,Puckeridge M,Liangfeng G,Tan YL,Jacob C,Garland M,Kuchel PW

更新日期：2013-11-05 00:00:00

abstract：:Measurements were made of exchange rates of oxygen, carbon dioxide, and krypton-85 with blood at 37.5 degrees C. Gas transfer took place across a 1 mil silicone rubber membrane. The blood was in a rotating disk boundary layer flow, and the controlling resistance to transfer was the concentration boundary layer. Measur...

journal_title：Biophysical journal

authors： Spaeth EE,Friedlander SK

更新日期：1967-11-01 00:00:00

abstract：:Although cell-cell interactions are known to significantly affect the kinetics of intracellular ice formation (IIF) during tissue freezing, this effect is not well understood. Progress in elucidating the mechanism and role of intercellular ice propagation in tissue freezing has been hampered in part by limitations in ...

journal_title：Biophysical journal

authors： Irimia D,Karlsson JO

更新日期：2005-01-01 00:00:00

abstract：:In the last decades, it has been demonstrated that many animal species orient in the Earth magnetic field. One of the best-studied examples is the use of the geomagnetic field by migratory birds for orientation and navigation. However, the biophysical mechanism underlying animal magnetoreception is still not understoo...

journal_title：Biophysical journal

authors： Solov'yov IA,Schulten K

更新日期：2009-06-17 00:00:00

abstract：:We studied the effects of permeant ions on the gating of the large conductance Ca(2+)-activated K+ channel from rat skeletal muscle. Rb+ blockade of inward K+ current caused an increase in the open probability as though Rb+ occupancy of the pore interferes with channel closing. In support of this hypothesis, we direct...

journal_title：Biophysical journal

authors： Demo SD,Yellen G

更新日期：1992-03-01 00:00:00

abstract：:Using a recently developed in vitro motility assay, we have demonstrated that local anesthetics directly inhibit myosin-based movement of single actin filaments in a reversible dose-dependent manner. This is the first reported account of the actions of local anesthetics on purified proteins at the molecular level. In ...

journal_title：Biophysical journal

authors： Tsuda Y,Mashimo T,Yoshiya I,Kaseda K,Harada Y,Yanagida T

更新日期：1996-11-01 00:00:00

abstract：:The chromophore of octopus rhodopsin is 11-cis retinal, linked via a protonated Schiff base to the protein backbone. Its stable photoproduct, metarhodopsin, has all-trans retinal as its chromphore. The Schiff base of acid metarhodopsin (lambda max = 510 nm) is protonated, whereas that of alkaline metarhodopsin (lambda...

journal_title：Biophysical journal

authors： Koutalos Y,Ebrey TG,Gilson HR,Honig B

更新日期：1990-08-01 00:00:00

abstract：:Recently, it has become clear that the influenza virus fusion protein, hemagglutinin (HA), produces membrane destabilization and fusion by a multistep process, which involves the aggregation of the HAs to form a fusion site. While the details of this process are under debate, it is important to recognize that proposin...

journal_title：Biophysical journal

authors： Bentz J

更新日期：1992-08-01 00:00:00

abstract：:It is shown that if x(t) is the solution of a second order differential equation, with real negative characteristic roots (not necessarily distinct), which exhibits an extremum at t = T, then T|x(T)|/|A| [UNK] 1/e where A is the area under the x(t) curve. This result is compared to a special case previously derived by...

journal_title：Biophysical journal

authors： HEARON JZ

更新日期：1961-09-01 00:00:00

abstract：:Segmentation of the Drosophila melanogaster embryo results from the dynamic establishment of spatial mRNA and protein patterns. Here, we exploit recent temporal mRNA and protein expression measurements on the full surface of the blastoderm to calibrate a dynamical model of the gap gene network on the entire embryo cor...

journal_title：Biophysical journal

authors： Bieler J,Pozzorini C,Naef F

更新日期：2011-07-20 00:00:00

abstract：:The salt dependence of the binding free energy of five protein-protein hetero-dimers and two homo-dimers/tetramers was calculated from numerical solutions to the Poisson-Boltzmann equation. Overall, the agreement with experimental values is very good. In all cases except one involving the highly charged lactoglobulin ...

journal_title：Biophysical journal

authors： Bertonati C,Honig B,Alexov E

更新日期：2007-03-15 00:00:00

abstract：:We have used chemical synthesis, electron paramagnetic resonance (EPR), and circular dichroism to detect and analyze the structural dynamics of a ryanodine receptor (RyR) peptide bound to calmodulin (CaM). The skeletal muscle calcium release channel RyR1 is activated by Ca2+-free CaM and inhibited by Ca2+-bound CaM. T...

journal_title：Biophysical journal

authors： Her C,McCaffrey JE,Thomas DD,Karim CB

更新日期：2016-12-06 00:00:00

abstract：:Morphine spin labeled on the phenolic hydroxy group has been prepared using commercially available reagents and characterized by thin layer chromatography, mass spectroscopy, and electron spin resonance spectroscopy. It has been shown that morphine modified in this way retains some opiate activity, does not pass throu...

journal_title：Biophysical journal

authors： Copeland ES,Boykin ME,Kelley JA,Kullberg MP

更新日期：1975-11-01 00:00:00

abstract：:A one-dimensional version of the model recently proposed by Läuger (1988) to explain the closed-time distribution of ionic channels in cell membranes is solved analytically. While the probability density f(t) for closed-time lengths may show a well-defined exponential behavior at short times, a power-law decay is pred...

journal_title：Biophysical journal

authors： Condat CA,Jäckle J

更新日期：1989-05-01 00:00:00

abstract：:The location of the photopigment molecules relative to the lipid hydrocarbon core of retinal receptor disk membranes was unknown. The photopigment molecules could occur entirely on the surface of the membrane, completely embedded in its hydrocarbon core, or at some intermediate location protruding into both the aqueou...

journal_title：Biophysical journal

authors： Blasie JK

更新日期：1972-02-01 00:00:00

abstract：:Normally, isothermal titration calorimetry (ITC) is used to study binding reactions between two different biomolecules. Self-association processes leading to homo-oligomeric complexes have usually not been studied by ITC; instead, methods such as spectroscopy and analytical ultracentrifugation, which only provide affi...

journal_title：Biophysical journal

authors： Luke K,Apiyo D,Wittung-Stafshede P

更新日期：2005-11-01 00:00:00

abstract：:We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment. The line FRAP method is developed to complement the disk FRAP method reported before. Although being more subject to the influe...

journal_title：Biophysical journal

authors： Braeckmans K,Remaut K,Vandenbroucke RE,Lucas B,De Smedt SC,Demeester J

更新日期：2007-03-15 00:00:00

abstract：:Fourier-transform infrared spectroscopy is a method of choice for the experimental determination of protein secondary structure. Numerous approaches have been developed during the past 15 years. A critical parameter that has not been taken into account systematically is the selection of the wavenumbers used for buildi...

journal_title：Biophysical journal

authors： Goormaghtigh E,Ruysschaert JM,Raussens V

更新日期：2006-04-15 00:00:00

abstract：:The presence of two liquid-crystalline phases, alpha and beta, in mixed bilayers of dimyristoylphosphatidylcholine/cholesterol was detected by the changes in the distribution of the fluorescence lifetimes of t-PnA, as analyzed by the Maximum Entropy Method. The formation of the liquid-ordered beta-phase, in the 30-40 ...

journal_title：Biophysical journal

authors： Reyes Mateo C,Ulises Acuña A,Brochon JC

更新日期：1995-03-01 00:00:00

abstract：:By employing classical molecular dynamics, correlation analysis of coupling between slow and fast dynamical modes, and free energy (umbrella) sampling using classical as well as mixed quantum mechanics molecular mechanics force fields, we uncover a possible pathway for phosphoryl transfer in the self-cleaving reaction...

journal_title：Biophysical journal

authors： Radhakrishnan R

更新日期：2007-10-01 00:00:00