NMR dynamics of PSE-4 beta-lactamase: an interplay of ps-ns order and mus-ms motions in the active site.

abstract：:We measured the viscoelastic properties of the cytoplasm of J774 macrophages with a recently developed microrheometer. Ferromagnetic beads (1.3 microm in diameter) were used to determine the local viscoelastic moduli. Step-force pulses were applied to the magnetic beads and the displacement was observed by single part...

journal_title：Biophysical journal

authors： Bausch AR,Möller W,Sackmann E

更新日期：1999-01-01 00:00:00

abstract：:Stationary and kinetic experiments were performed on lipid bilayer membranes to study the mechanism of iodine- and bromine-mediated halide transport in detail. The stationary conductance data suggested that four different 1:1 complexes between I2 and Br2 and the halides I- and Br- were responsible for the observed con...

journal_title：Biophysical journal

authors： Klotz KH,Benz R

更新日期：1993-12-01 00:00:00

abstract：:Viscoelastometric measurements of DNA from gamma-irradiated bacteria were used to identify the induction of double-strand breaks ( DSBs ) in the chromosome of Escherichia coli. It is shown by means of inhibitors of repair endonucleases and different repair mutants that most DSBs in DNA of E. coli, gamma-irradiated in ...

journal_title：Biophysical journal

authors： Bresler SE,Noskin LA,Suslov AV

更新日期：1984-04-01 00:00:00

abstract：:A Brownian dynamics (BD) model described in the accompanying paper (Klenin, K., H. Merlitz, and J. Langowski. 1998. A Brownian dynamics program for the simulation of linear and circular DNA, and other wormlike chain polyelectrolytes. Biophys. J. 74:000-000) has been used for computing the end-to-end distance distribut...

journal_title：Biophysical journal

authors： Merlitz H,Rippe K,Klenin KV,Langowski J

更新日期：1998-02-01 00:00:00

abstract：:We describe Franck-Condon simulations of vibrational cooling effects on absorption difference spectra in chlorophyll a (Chl a). The relative contributions of vibrational equilibration in the electronic ground and excited states depend on the pump and probe wavelengths. For Franck-Condon-active vibrational modes exhibi...

journal_title：Biophysical journal

authors： Struve WS

更新日期：1995-12-01 00:00:00

abstract：:Fundamental mechanisms governing cell size control and homeostasis are still poorly understood. The relationship between sizes at division and birth in single cells is used as a metric to categorize the basis of size homeostasis. Cells dividing at a fixed size regardless of birth size (sizer) are expected to show a di...

journal_title：Biophysical journal

authors： Facchetti G,Knapp B,Chang F,Howard M

更新日期：2019-11-05 00:00:00

abstract：:In the first issue, on the first page of the Biophysical Journal in 1960, Cole and Moore provided the first confirmation of the Hodgkin and Huxley formulation of the sodium and potassium conductances that underlie the action potential. In addition, working with the squid giant axon, Cole and Moore noted that strong hy...

journal_title：Biophysical journal

authors： Hoshi T,Armstrong CM

更新日期：2015-10-06 00:00:00

abstract：:Molecular sensors based on intramolecular Förster resonance energy transfer (FRET) have become versatile tools to monitor regulatory molecules in living tissue. However, their use is often compromised by low signal strength and excessive noise. We analyzed signal/noise (SNR) aspects of spectral FRET analysis methods, ...

journal_title：Biophysical journal

authors： Woehler A,Wlodarczyk J,Neher E

更新日期：2010-10-06 00:00:00

abstract：:The high gradient magnetic separation technique has been applied to separate paramagnetic erythrocytes from a cell suspension that also contained diamagnetic cells. Paramagnetism was induced in the red blood cells by oxidizing the iron atoms in the cell hemoglobin to the ferric state (methemoglobin). Diamagnetic cells...

journal_title：Biophysical journal

authors： Owen CS

更新日期：1978-05-01 00:00:00

abstract：:The resonance Raman spectrum of the second intermediate in the bacteriorhodopsin cycle, bL550, is obtained by a simple flow technique. The Schiff base linkage in this intermediate appears to be protonated, contrary to previous suggestion. The fingerprint region of the spectrum of bL550 does not closely match those of ...

journal_title：Biophysical journal

authors： Terner J,Hsieh CL,El-Sayed MA

更新日期：1979-06-01 00:00:00

abstract：:A recently developed technique for in vivo determination of the electrical resistance of vascular endothelium in microvessels was applied to the vessels in a thin frog muscle, m. cutaneus pectoris. The technique consists of injection of current via a glass micropipette into a capillary and measurement of the resulting...

journal_title：Biophysical journal

authors： Olesen SP,Crone C

更新日期：1983-04-01 00:00:00

abstract：:The bacterial flagellar motor couples the flow of protons across the cytoplasmic membrane to the rotation of a helical flagellar filament. Using tethered cells, we have measured the stall torque required to block this rotation and compared it with the torque of the running motor over a wide range of values of proton-m...

journal_title：Biophysical journal

authors： Meister M,Berg HC

更新日期：1987-09-01 00:00:00

abstract：:We have applied two-dimensional infrared (2D IR) and betanu correlation spectroscopy to in-situ IR spectroscopy of pulmonary surfactant proteins SP-B and SP-C in lipid-protein monolayers at the air-water interface. For both SP-B and SP-C, a statistical windowed autocorrelation method identified two separate surface pr...

journal_title：Biophysical journal

authors： Shanmukh S,Howell P,Baatz JE,Dluhy RA

更新日期：2002-10-01 00:00:00

abstract：:It is now known that the flexibility of the troponin T (TnT) tail determines thin filament conformation and hence cross-bridge cycling properties, expanding the classic structural role of TnT to a dynamic role regulating sarcomere function. Here, using transgenic mice bearing R-92W and R-92L missense mutations in card...

journal_title：Biophysical journal

authors： He H,Javadpour MM,Latif F,Tardiff JC,Ingwall JS

更新日期：2007-09-01 00:00:00

abstract：:The piezoelectric properties of single collagen type I fibrils in fascia were imaged with sub-20 nm spatial resolution using piezoresponse force microscopy. A detailed analysis of the piezoresponse force microscopy signal in controlled tip-fibril geometry revealed shear piezoelectricity parallel to the fibril axis. Th...

journal_title：Biophysical journal

authors： Harnagea C,Vallières M,Pfeffer CP,Wu D,Olsen BR,Pignolet A,Légaré F,Gruverman A

更新日期：2010-06-16 00:00:00

abstract：:Interactions between proteins are often sufficiently weak that their study through the use of conventional structural techniques becomes problematic. Of the few techniques capable of providing experimental measures of weak protein-protein interactions, perhaps the most useful is the second virial coefficient, B(22), w...

journal_title：Biophysical journal

authors： Elcock AH,McCammon JA

更新日期：2001-02-01 00:00:00

abstract：:We report that voltage-gated Na+ channels (Na(V)) from rat muscle (mu1) expressed in HEK293 cells exhibit anomalous rectification of whole-cell outward current under conditions of symmetrical Na+. This behavior gradually fades with time after membrane break-in, as if a diffusible blocking substance in the cytoplasm is...

journal_title：Biophysical journal

authors： Huang CJ,Moczydlowski E

更新日期：2001-03-01 00:00:00

abstract：:Cochlear outer hair cells undergo reversible changes in shape when externally stimulated. This response, known as OHC motility, is a central component of the cochlear amplifier, the mechanism responsible for the high sensitivity of mammalian hearing. We report that actin depolymerization, as regulated by activation/in...

journal_title：Biophysical journal

authors： Matsumoto N,Kitani R,Maricle A,Mueller M,Kalinec F

更新日期：2010-10-06 00:00:00

abstract：:Adsorption of lambda-phage on sensitive bacteria Escherichia coli is a classical problem but not all issues have been resolved. One of the outstanding problems is the rate of adsorption, which in some cases appears to exceed the theoretical limit imposed by the law of random diffusion. We revisit this problem by condu...

journal_title：Biophysical journal

authors： Moldovan R,Chapman-McQuiston E,Wu XL

更新日期：2007-07-01 00:00:00

abstract：:Many approaches for studying the transmembrane potential (TMP) induced during the treatment of biological cells with pulsed electric fields have been reported. From the simple analytical models to more complex numerical models requiring significant computational resources, a gamut of methods have been used to recapitu...

journal_title：Biophysical journal

authors： Murovec T,Sweeney DC,Latouche E,Davalos RV,Brosseau C

更新日期：2016-11-15 00:00:00

abstract：:The conformational flexibility of the DNA double helix is of great interest because of its potential role in protein recognition, packaging into chromosomes, formation of photodefects, and interaction with drugs. Theory finds that DNA is very flexible; however, there is a scarcity of experimental results that examine ...

journal_title：Biophysical journal

authors： Georghiou S,Bradrick TD,Philippetis A,Beechem JM

更新日期：1996-04-01 00:00:00

abstract：:The influence of the amino acid residues surrounding the flavin ring in the flavodoxin of the cyanobacterium Anabaena PCC 7119 on the electron spin density distribution of the flavin semiquinone was examined in mutants of the key residues Trp(57) and Tyr(94) at the FMN binding site. Neutral semiquinone radicals of the...

journal_title：Biophysical journal

authors： Medina M,Lostao A,Sancho J,Gómez-Moreno C,Cammack R,Alonso PJ,Martínez JI

更新日期：1999-09-01 00:00:00

abstract：:We characterized the effect of the first basepair on the conformational dynamics of the fluorescent dye Cy3 attached to the 5' end of double-stranded DNA using gaussian-mixture adaptive umbrella sampling simulations. In the simulations, the sampling of all five dihedral angles along the linker was enhanced, so that bo...

journal_title：Biophysical journal

authors： Spiriti J,Binder JK,Levitus M,van der Vaart A

更新日期：2011-02-16 00:00:00

abstract：:There is a steadily growing body of experimental data describing the diffusion of acetylcholine in the neuromuscular junction and the subsequent miniature endplate currents produced at the postsynaptic membrane. To gain further insights into the structural features governing synaptic transmission, we have performed ca...

journal_title：Biophysical journal

authors： Smart JL,McCammon JA

更新日期：1998-10-01 00:00:00

abstract：:The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho- and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (Lo or lipid...

journal_title：Biophysical journal

authors： Abbandonato G,Storti B,Tonazzini I,Stöckl M,Subramaniam V,Montis C,Nifosì R,Cecchini M,Signore G,Bizzarri R

更新日期：2019-02-05 00:00:00

abstract：:Surface charge in track-etched polyethylene terephthalate (PET) membranes with narrow pores has been probed with a fluorescent cationic dye (3,3'-diethyloxacarbocyanine iodide (diO-C2-(3))) using confocal microscopy. Staining of negatively charged PET membranes with diO-C2-(3) is a useful measure of surface charge for...

journal_title：Biophysical journal

authors： Bashford CL,Alder GM,Pasternak CA

更新日期：2002-04-01 00:00:00

abstract：:The [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii can catalyze the reduction of protons to hydrogen gas using electrons supplied from photosystem I and transferred via ferredoxin. To better understand the association of the hydrogenase and the ferredoxin, we have simulated the process over multiple ...

journal_title：Biophysical journal

authors： Long H,Chang CH,King PW,Ghirardi ML,Kim K

更新日期：2008-10-01 00:00:00

abstract：:Exposure of aqueous suspensions of fragments of the purple membrane of Halobacterium halobium to electric field pulses leads to transient linear dichroism phenomena. The effects are interpreted in terms of field-induced alignments of the bacteriorhodopsin chromophore. Two observed relaxation times (tau) are attributed...

journal_title：Biophysical journal

authors： Shinar R,Druckmann S,Ottolenghi M,Korenstein R

更新日期：1977-07-01 00:00:00

abstract：:Effects of intracellular ionic strength on the isotonic contraction properties of both intact fibers and skinned fibers give insights into the cross-bridge mechanism, but presently there is fundamental disagreement in the results on the two fiber preparations. This paper, which studies the effects on contraction of va...

journal_title：Biophysical journal

authors： Gulati J,Babu A

更新日期：1984-02-01 00:00:00

abstract：:Previous data as well as new results are examined with a view to determining the boundary conditions which present experimental information places on a satisfactory polypeptide chain model for the structure of feather keratin. Our studies indicate these conditions to include the following: (1) A 189 A identity period,...

journal_title：Biophysical journal

authors： Schor R,Krimm S

更新日期：1961-07-01 00:00:00