Abstract：
:A recently developed technique for in vivo determination of the electrical resistance of vascular endothelium in microvessels was applied to the vessels in a thin frog muscle, m. cutaneus pectoris. The technique consists of injection of current via a glass micropipette into a capillary and measurement of the resulting intra- and extravascular potential profiles with another micropipette placed at various distances from the current source. The theory of Peskoff and Eisenberg (1974) was used to handle the problems arising from distributed extravascular resistances and was experimentally shown to describe the external field satisfactorily. With this extension of one-dimensional cable theory the specific electrical resistance of arterial microvessels was 33 omega cm2 and of venous capillaries 23 omega cm2. The "length constants" were 135 and 112 micrometers, respectively. If results from arterial and venous vessels are taken together, the ionic permeabilities at 20 degrees C were PNa = 3.9 X 10(-5) cm X s-1, PK = 5.7 X 10(-5) cm X s-1, PCl = 5.9 X 10(-5) cm X s-1 and PHCO3 = 3.4 X 10(-5) cm X s-1. These figures agree with figures for capillary permeability obtained in tracer experiments on whole muscle. The study bridges a gap between single capillary and whole organ techniques with the conclusion that the two different approaches lead to similar results in muscle capillaries.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Olesen SP,Crone Cdoi
10.1016/S0006-3495(83)84366-5subject
Has Abstractpub_date
1983-04-01 00:00:00pages
31-41issue
1eissn
0006-3495issn
1542-0086pii
S0006-3495(83)84366-5journal_volume
42pub_type
杂志文章abstract：:High speed cinemicrographs were made of sea urchin sperm at temperatures varying from 22 to 6 degrees C. Apparatus, combining a television camera and a video digitizer, was constructed to scan individual flagellar images and to digitize the flagellar waveforms. With appropriate smoothing and averaging procedures, the ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(85)83931-X
更新日期：1985-03-01 00:00:00
abstract：:Protein solution rheology data in the biophysics literature have incompletely identified factors that govern hydrodynamics. Whereas spontaneous protein adsorption at the air/water (A/W) interface increases the apparent viscosity of surfactant-free globular protein solutions, it is demonstrated here that irreversible c...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2013.01.007
更新日期：2013-02-19 00:00:00
abstract：:In the absence of atomic structures of high-density lipoproteins in their lipid-bound states, many molecular models have been produced based on experimental data. Using molecular dynamics, we show that a recently proposed "solar-flares" model of discoidal high-density lipoprotein is implausible. Our simulations show a...
journal_title：Biophysical journal
pub_type： 信件
doi：10.1529/biophysj.108.131581
更新日期：2008-06-01 00:00:00
abstract：:Exchangeable apolipoproteins are located in the surface of lipoprotein particles and regulate lipid metabolism through direct protein-protein and protein-lipid interactions. These proteins are characterized by the presence of tandem repeats of amphiphatic alpha-helix segments and a high surface activity in monolayers ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.107.115220
更新日期：2008-03-01 00:00:00
abstract：:The glucose transporters (GLUT/SLC2A) are members of the major facilitator superfamily. Here, we generated a three-dimensional model for Glut1 using a two-step strategy: 1), GlpT structure as an initial homology template and 2), evolutionary homology using glucose-6-phosphate translocase as a template. The resulting s...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.104.047886
更新日期：2004-11-01 00:00:00
abstract：:Recent experimental evidence from a number of preparations indicates that sodium channel inactivation may be intrinsically voltage sensitive. Intrinsically voltage sensitive inactivation should produce a charge movement. Crayfish giant axons provide a unique opportunity to reexamine the slower components of gating cur...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(83)84434-8
更新日期：1983-03-01 00:00:00
abstract：:Depending on the nucleotide sequence, the temperature, and other conditions, RNA hairpin-folding kinetics can be very complex. The complexity with a wide range of cooperative and noncooperative kinetic behaviors arises from the interplay between the formation of the loops, the disruption of the misfolded states, and t...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.105.062935
更新日期：2006-02-01 00:00:00
abstract：:Adsorption-desorption isotherms were obtained for water binding by 1,2-dimyristoylphosphatidylcholine in the temperature range 15 degrees-35 degrees C. The isotherms were analyzed by Brunauer et al.'s (BET) theory and also a polarization theory, the latter being more successful in fitting the data. There was some evid...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(77)85542-2
更新日期：1977-11-01 00:00:00
abstract：:The mechanism of pH-triggered destabilization of liposomes composed of a polyethyleneglycol-orthoester-distearoylglycerol lipid (POD) and phosphatidyl ethanolamine (PE) has been studied using an ANTS/DPX leakage and a lipid-mixing assay. We developed a kinetic model that relates POD hydrolysis to liposome collapse. Th...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/s0006-3495(03)74986-8
更新日期：2003-03-01 00:00:00
abstract：:Cyclic 3'5' adenosine monophosphate (cAMP)-dependent-protein kinase (PKA) signaling is a fundamental regulatory pathway for mediating cellular responses to hormonal stimuli. The pathway is activated by high-affinity association of cAMP with the regulatory subunit of PKA and signal termination is achieved upon cAMP dis...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2014.07.050
更新日期：2014-09-16 00:00:00
abstract：:The mechanism for transport of divalent cations across phospholipid bilayers by the ionophore A23187 was investigated. The intrinsic fluorescence of the ionophore was used in equilibrium and rapid-mixing experiments as an indicator of ionophore environment and complexation with divalent cations. The neutral (protonate...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(81)84738-8
更新日期：1981-11-01 00:00:00
abstract：:Multiconformation membrane proteins are mechanosensitive (MS) if their conformations displace different bilayer areas. Might MS closed-closed transitions serve as tension buffers, that is, as membrane "spandex"? While bilayer expansion is effectively instantaneous, transitions of bilayer-embedded MS proteins are stoch...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2009.08.054
更新日期：2009-11-18 00:00:00
abstract：:Epithelial injury induces rapid recruitment of antimicrobial leukocytes to the wound site. In zebrafish larvae, activation of the epithelial NADPH oxidase Duox at the wound margin is required early during this response. Before injury, leukocytes are near the vascular region, that is, ∼100-300 μm away from the injury s...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2017.03.021
更新日期：2017-05-09 00:00:00
abstract：:Articular cartilage is the connective tissue that lines joints and provides a smooth surface for joint motion. Because cartilage is avascular, molecular transport occurs primarily via diffusion or convection, and cartilage matrix structure and composition may affect diffusive transport. Because of the inhomogeneous co...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.108.137752
更新日期：2008-11-15 00:00:00
abstract：:The influence of patch electrode glass composition on macroscopic IV relations in inside-out patches of the cGMP-activated ion channel from rod photoreceptors was examined for a soda lime glass, a Kovar sealing glass, a borosilicate glass, and several soft lead glasses. In several glasses the shape or magnitude of the...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(88)83091-1
更新日期：1988-02-01 00:00:00
abstract：:The published reports of flash-induced absorbance changes in the 680-690 nm spectral region, which have been attributed to bleaching of the primary reaction center chlorophyll of photosystem II (PSII) P-680, are discussed in light of what is known about the primary electron acceptor of PSII, C-550. The question of whe...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(72)86128-9
更新日期：1972-07-01 00:00:00
abstract：:A single isolated sinoatrial pacemaker cell presents intrinsic interbeat interval (IBI) variability that is believed to result from the stochastic characteristics of the opening and closing processes of membrane ion channels. To our knowledge, a novel mathematical framework was developed in this work to address the ef...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2015.01.010
更新日期：2015-03-10 00:00:00
abstract：:The interaction between protein molecules and the hydroxyapatite (HAP) crystal is an important research topic in many fields. However, the nature of their noncovalent bonding is still not clear at the atomic level. In this work, molecular dynamics simulation, steered molecular dynamics simulation, and quantum chemistr...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.106.103168
更新日期：2007-08-01 00:00:00
abstract：:We studied the cold unfolding of myoglobin with Fourier transform infrared spectroscopy and compared it with pressure and heat unfolding. Because protein aggregation is a phenomenon with medical as well as biotechnological implications, we were interested in both the structural changes as well as the aggregation behav...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(02)75605-1
更新日期：2002-05-01 00:00:00
abstract：:The Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzymes regulated by a voltage-sensor domain (VSD) related to the VSD found in voltage-gated ion channels. Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics compared to ion chann...
journal_title：Biophysical journal
pub_type： 信件
doi：10.1016/j.bpj.2012.06.030
更新日期：2012-08-08 00:00:00
abstract：:Calcium is essential for many biological processes involved in cellular motility. However, the pathway by which calcium influences motility, in processes such as muscle contraction and neuronal growth, is often indirect and complex. We establish a simple and direct mechanochemical link that shows how calcium quantitat...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2009.02.069
更新日期：2009-08-19 00:00:00
abstract：:The salt dependence of the binding free energy of five protein-protein hetero-dimers and two homo-dimers/tetramers was calculated from numerical solutions to the Poisson-Boltzmann equation. Overall, the agreement with experimental values is very good. In all cases except one involving the highly charged lactoglobulin ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.106.092122
更新日期：2007-03-15 00:00:00
abstract：:The kinetics of conformational changes of P-type ATPases necessary for the occlusion or deocclusion of transported ions are known to be sensitive to the composition of the surrounding membrane, e.g., phospholipid content, mole percentage of cholesterol, and the presence of lipid-bound anions. Research has shown that m...
journal_title：Biophysical journal
pub_type： 杂志文章,评审
doi：10.1016/j.bpj.2015.08.022
更新日期：2015-10-20 00:00:00
abstract：:The interactions of the hydrophobic pulmonary surfactant proteins SP-B and SP-C with 1,2-dipalmitoylphosphatidylcholine in mixed, spread monolayer films have been studied in situ at the air/water interface with the technique of external reflection absorption infrared spectroscopy (IRRAS). SP-C has a mostly alpha-helic...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(95)80124-4
更新日期：1995-12-01 00:00:00
abstract：:Certain standard properties, including spontaneous curvature, are assumed for the membrane of a bilipid vesicle. Then, if there is a mechanism that causes the membrane area to increase, vesicle cleavage is found to occur. ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/s0006-3495(79)85280-7
更新日期：1979-01-01 00:00:00
abstract：:There are several examples of membrane-associated protein domains that target curved membranes. This behavior is believed to have functional significance in a number of essential pathways, such as clathrin-mediated endocytosis, which involve dramatic membrane remodeling and require the recruitment of various cofactors...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2011.01.036
更新日期：2011-03-02 00:00:00
abstract：:Proteolysis of the multimeric blood coagulation protein von Willebrand Factor (VWF) by ADAMTS13 is crucial for prevention of microvascular thrombosis. ADAMTS13 cleaves VWF within the mechanosensitive A2 domain, which is believed to open under shear flow. In this study, we combine fluorescence correlation spectroscopy ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2015.12.023
更新日期：2016-02-02 00:00:00
abstract：:Dielectrophoresis and electrorotation are commonly used to measure dielectric properties and membrane electrical parameters of biological cells. We have derived quantitative relationships for several critical points, defined in Fig. A 1, which characterize the dielectrophoretic spectrum and the electrorotational spect...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(91)82109-9
更新日期：1991-10-01 00:00:00
abstract：:To characterize elastic properties and geometrical parameters of individual, whole myosin molecules during their interaction with actin we sparsely adsorbed myosin molecules to nanometer-sized microspheres. Thermally driven position fluctuations of these microspheres were recorded with the three-dimensional detection ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.104.047795
更新日期：2005-01-01 00:00:00
abstract：:The excess oxygen concentration in the photosynthetic membranes of functioning oxygenic photosynthetic cells was estimated using classical diffusion theory combined with experimental data on oxygen production rates of cyanobacterial cells. The excess oxygen concentration within the plesiomorphic cyanobacterium Gloeoba...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2014.03.031
更新日期：2014-05-06 00:00:00
