Order parameters of a transmembrane helix in a fluid bilayer: case study of a WALP peptide.

abstract：:The photocycle of salinarum halorhodopsin was investigated in the presence of azide. The azide binds to the halorhodopsin with 150 mM binding constant in the absence of chloride and with 250 mM binding constant in the presence of 1 M chloride. We demonstrate that the azide-binding site is different from that of chlori...

journal_title：Biophysical journal

authors： Lakatos M,Groma GI,Ganea C,Lanyi JK,Váró G

更新日期：2002-04-01 00:00:00

abstract：:Some low molecular weight biomolecules, i.e., NAD(P)H, are unstable at high temperatures. The use of these biomolecules by thermophilic microorganisms has been scarcely analyzed. Herein, NADH stability has been studied at different temperatures and viscosities. NADH decay increased at increasing temperatures. At incre...

journal_title：Biophysical journal

authors： Cuecas A,Cruces J,Galisteo-López JF,Peng X,Gonzalez JM

更新日期：2016-08-23 00:00:00

abstract：:We have developed a method of performing near-field fluorescence correlation spectroscopy via an array of planarized circular apertures of 50 nm diameter. This technique provides 1 μs and 60 nm resolution on proximal samples, including live cells, without incorporating a scanning probe or pulsed lasers or requiring pe...

journal_title：Biophysical journal

authors： Kelly CV,Baird BA,Craighead HG

更新日期：2011-04-06 00:00:00

abstract：:We have used anomalous small-angle x-ray scattering as a structural probe for solutions of rabbit parvalbumin labeled with terbium. This technique makes use of the large changes in the terbium scattering factor that occur when the x-ray energy is tuned around an L3 absorption edge of this heavy-atom label. These chang...

journal_title：Biophysical journal

authors： Miake-Lye RC,Doniach S,Hodgson KO

更新日期：1983-03-01 00:00:00

abstract：:In this study we describe the expression and function of the two rat type-1 inositol 1,4,5-trisphosphate receptor (InsP3R) ligand binding domain splice variants (SI+/-/SII+). Receptor protein from COS-1 cells transfected with the type-1 InsP3R expression plasmids (pInsP3R-T1, pInsP3R-T1ALT) or control DNA were incorpo...

journal_title：Biophysical journal

authors： Ramos-Franco J,Caenepeel S,Fill M,Mignery G

更新日期：1998-12-01 00:00:00

abstract：:Chromatin organization within the nucleus is a vital regulator of genome function, yet its mechanical coupling to the nuclear architecture has remained elusive. To directly investigate this coupling, we locally modulated chromatin structure in living cells using nanoparticle-based laser perturbation. Unusual differenc...

journal_title：Biophysical journal

authors： Mazumder A,Shivashankar GV

更新日期：2007-09-15 00:00:00

abstract：:A detailed model for the kinetics and energetics of the exciton trapping, charge separation, charge recombination, and charge stabilization processes in photosystem (PS) II is presented. The rate constants describing these processes in open and closed reaction centers (RC) are calculated on the basis of picosecond dat...

journal_title：Biophysical journal

authors： Schatz GH,Brock H,Holzwarth AR

更新日期：1988-09-01 00:00:00

abstract：:In addition to being the gateway for all access to the eukaryotic genome, chromatin has in recent years been identified as carrying an epigenetic code regulating transcriptional activity. Though much is known about the biochemistry of this code, little is understood regarding the different fiber structures through whi...

journal_title：Biophysical journal

authors： Depken M,Schiessel H

更新日期：2009-02-01 00:00:00

abstract：:Formation of protrusions and protein segregation on the membrane is of a great importance for the functioning of the living cell. This is most evident in recent experiments that show the effects of the mechanical properties of the surrounding substrate on cell morphology. We propose a mechanism for the formation of me...

journal_title：Biophysical journal

authors： Veksler A,Gov NS

更新日期：2007-12-01 00:00:00

abstract：:Spin-label saturation transfer EPR (ST-EPR) methods have been used to study the sickle hemoglobin (HbS) aggregation behaviors induced by slow deoxygenation at a constant temperature of 30 degrees C, and by a rapid temperature increase from 1 degree to 30 degrees C for fully deoxygenated HbS. For slow deoxygenation at ...

journal_title：Biophysical journal

authors： Thiyagarajan P,Johnson ME

更新日期：1983-06-01 00:00:00

abstract：:Slow fluorescence transients in Chlamydomonas reinhardi arise after transitions from high light intensities to low light or dark conditions. Characteristics of the newly described transient phenomena include: (a) A slow biphasic decrease in fluorescence yield occurs in the dark, followed by an even slower, hour long, ...

journal_title：Biophysical journal

authors： Rice GE,Bannister TT

更新日期：1980-11-01 00:00:00

abstract：:The interaction of an amphiphilic, 40-amino acid beta-amyloid (Abeta) peptide with liposomal membranes as a function of sterol mole fraction (X(sterol)) was studied based on the fluorescence anisotropy of a site-specific membrane sterol probe, dehydroergosterol (DHE), and fluorescence resonance energy transfer (FRET) ...

journal_title：Biophysical journal

authors： Qiu L,Lewis A,Como J,Vaughn MW,Huang J,Somerharju P,Virtanen J,Cheng KH

更新日期：2009-05-20 00:00:00

abstract：:Deuterium and phosphorus nuclear magnetic resonance (NMR) has been used to investigate the dynamics of slow motional processes induced in bilayer cardiolipin upon binding with cytochrome c. 31P NMR line shapes suggest that protein binding induces less restricted, isotropic-like motions in the lipid phosphates within t...

journal_title：Biophysical journal

authors： Spooner PJ,Duralski AA,Rankin SE,Pinheiro TJ,Watts A

更新日期：1993-07-01 00:00:00

abstract：:Alkaline sucrose sedimentation studies of DNA from mouse lymphoma cells (L5178Y) treated with caffeine have demonstrated the following effects. Caffeine (at a concentration of 1.6 mM) does not introduce strand breaks into preformed DNA nor does it inhibit the rejoining of gamma-ray-induced strand breaks. Although it d...

journal_title：Biophysical journal

authors： Lehmann AR

更新日期：1972-10-01 00:00:00

abstract：:The lipid matrix present in the uppermost layer of the skin, the stratum corneum, plays a crucial role in the skin barrier function. The lipids are organized into two lamellar phases. To gain more insight into the molecular organization of one of these lamellar phases, we performed neutron diffraction studies. In the ...

journal_title：Biophysical journal

authors： Groen D,Gooris GS,Barlow DJ,Lawrence MJ,van Mechelen JB,Demé B,Bouwstra JA

更新日期：2011-03-16 00:00:00

abstract：:Cells control organelle size with great precision and accuracy to maintain optimal physiology, but the mechanisms by which they do so are largely unknown. Cilia and flagella are simple organelles in which a single measurement, length, can represent size. Maintenance of flagellar length requires an active transport pro...

journal_title：Biophysical journal

authors： Ludington WB,Ishikawa H,Serebrenik YV,Ritter A,Hernandez-Lopez RA,Gunzenhauser J,Kannegaard E,Marshall WF

更新日期：2015-03-24 00:00:00

abstract：:Cytochrome c3 (cyt c3) can mediate electron transport across phosphatidylcholine (PC)/cardiolipin (CL) and PC/phosphatidylglycerol (PG) membranes. A two-molecule process is involved in the electron transport across PC/CL membranes in the liquid-crystalline state. In contrast, a single-molecule process dominates the el...

journal_title：Biophysical journal

authors： Kim SM,Yamamoto T,Todokoro Y,Takayama Y,Fujiwara T,Park JS,Akutsu H

更新日期：2006-01-15 00:00:00

abstract：:Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one c...

journal_title：Biophysical journal

authors： Merkel R,Kirchgessner N,Cesa CM,Hoffmann B

更新日期：2007-11-01 00:00:00

abstract：:The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of e...

journal_title：Biophysical journal

authors： Achey PM,Courtney DG

更新日期：1973-01-01 00:00:00

abstract：:The cytoskeletal activity of motile or adherent cells is frequently seen to induce detectable displacements of sufficiently compliant substrata. The physics of this phenomenon is discussed in terms of the classical theory of small-strain, plane-stress elasticity. The main results of such analysis is a transform expres...

journal_title：Biophysical journal

authors： Dembo M,Oliver T,Ishihara A,Jacobson K

更新日期：1996-04-01 00:00:00

abstract：:The results of variable dielectric coefficient Poisson-Boltzmann calculations of the counter-ion concentration in the vicinity of an all-atom model of the B-form of DNA are presented with an emphasis on the importance of spatial variations in the dielectric properties of the solvent, particularly at the macro-ion-solv...

journal_title：Biophysical journal

authors： Pack GR,Garrett GA,Wong L,Lamm G

更新日期：1993-10-01 00:00:00

abstract：:Kinesin and cytoplasmic dynein are microtubule-based motor proteins that actively transport material throughout the cell. Microtubules can intersect at a variety of angles both near the nucleus and at the cell periphery, and the behavior of molecular motors at these intersections has implications for long-range transp...

journal_title：Biophysical journal

authors： Ross JL,Shuman H,Holzbaur EL,Goldman YE

更新日期：2008-04-15 00:00:00

abstract：:Chinese hamster ovary cells were irradiated with 100-5,000 rads of X-rays and inhibition of the initiation of replicons after irradiation was demonstrated by analyzing nascent DNA sedimented in alkaline sucrose gradients. The renaturation kinetics of DNA synthesized during 60 min of incubation after irradiation was co...

journal_title：Biophysical journal

authors： Mattern MR,Painter RB

更新日期：1977-08-01 00:00:00

abstract：:Cellular membranes are laterally organized into domains of distinct structures and compositions by the differential interaction affinities between various membrane lipids and proteins. A prominent example of such structures are lipid rafts, which are ordered, tightly packed domains that have been widely implicated in ...

journal_title：Biophysical journal

authors： Lin X,Gorfe AA,Levental I

更新日期：2018-04-24 00:00:00

abstract：:Thermal inactivation of T(4) and lambda bacteriophage shows two components of differing sensitivity are present. These cannot be interpreted as owing to nucleic acid and protein. One protein function-the inhibition of radiation-induced DNA degradation-is lost with quite different thermal kinetics. lambda heated in the...

journal_title：Biophysical journal

authors： Pollard EC,Solosko W

更新日期：1971-01-01 00:00:00

abstract：:A simple theoretical analysis shows that specificity of double-stranded DNA (dsDNA) targeting by homopyrimidine peptide nucleic acids (hpyPNAs) is a kinetically controlled phenomenon. Our computations give the optimum conditions for sequence-specific targeting of dsDNA by hpyPNAs. The analysis shows that, in agreement...

journal_title：Biophysical journal

authors： Demidov VV,Yavnilovich MV,Frank-Kamenetskii MD

更新日期：1997-06-01 00:00:00

abstract：:The effect of cross-bridge clustering and head-head competition on the mechanical response of skeletal muscle under equilibrium conditions is considered. For this purpose, the recent multiple site equilibrium cross-bridge model of Schoenberg (Schoenberg, M., 1985, Biophys. J., 48:467-475) is extended in accordance wit...

journal_title：Biophysical journal

authors： Tözeren A,Schoenberg M

更新日期：1986-11-01 00:00:00

abstract：:The rotation of the lever arm of myosin cross-bridges is believed to be responsible for muscle contraction. To resolve details of this rotation, it is necessary to observe a single cross-bridge. It is still impossible to do so in muscle fiber, but it is possible to investigate a small population of cross-bridges by si...

journal_title：Biophysical journal

authors： Borejdo J,Shepard A,Akopova I,Grudzinski W,Malicka J

更新日期：2004-12-01 00:00:00

abstract：:The reaction of c-cytochromes with iron hexacyanides is similar in mechanism to the interaction of cytochromes with their physiological oxidants and reductants in that the formation of complexes precedes electron transfer. Analysis of the kinetics of oxidation and reduction of a number of c-cytochromes by solving the ...

journal_title：Biophysical journal

authors： Ohno N,Cusanovich MA

更新日期：1981-12-01 00:00:00

abstract：:In the rabbit corneal stroma, the swelling pressure, P, has been reported to have an anomalous (negative) temperature coefficient, alpha P, contradicting traditional Donnan swelling theory. A parallel-plate, diffuse double layer Gouy-Chapman model was used to resolve this discrepancy. The present model incorporates th...

journal_title：Biophysical journal

authors： Kwok LS,Klyce SD

更新日期：1990-03-01 00:00:00