Abstract:
:Spin-label saturation transfer EPR (ST-EPR) methods have been used to study the sickle hemoglobin (HbS) aggregation behaviors induced by slow deoxygenation at a constant temperature of 30 degrees C, and by a rapid temperature increase from 1 degree to 30 degrees C for fully deoxygenated HbS. For slow deoxygenation at 30 degrees C, we find that the effective HbS correlation time exhibits a continuous increase, without any abrupt transitions, suggesting that polymer formation in concentrated HbS at high temperature occurs even at high oxygenation levels. Upon a rapid temperature increase, fully deoxygenated HbS exhibits a short delay time, then an abrupt increase in effective correlation time. These results also indicate that ST-EPR provides a useful method for probing the molecular dynamics of HbS aggregation.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Thiyagarajan P,Johnson MEdoi
10.1016/S0006-3495(83)84394-Xsubject
Has Abstractpub_date
1983-06-01 00:00:00pages
269-74issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(83)84394-Xjournal_volume
42pub_type
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