The molecular basis of the solution properties of hyaluronan investigated by confocal fluorescence recovery after photobleaching.

abstract：:The dependence of the fluorescence of catalase upon the concentration of added superoxide dismutase (SOD) indicates that SOD binds to saturable sites on catalase. The affinity of SOD for these sites varies with temperature, and with the concentration of each of three nominally inert polymeric additives--dextran 70, Fi...

journal_title：Biophysical journal

authors： Jiao M,Li HT,Chen J,Minton AP,Liang Y

更新日期：2010-08-04 00:00:00

abstract：:The muscle nicotinic receptor (AChR) is a pentamer of four different subunits, each of which contains four transmembrane domains (M1-M4). We recently showed that channel opening and closing rates of the AChR depend on a hydrogen bond involving a threonine at position 14' of the M4 domain in the alpha-subunit. To deter...

journal_title：Biophysical journal

authors： Bouzat C,Gumilar F,del Carmen Esandi M,Sine SM

更新日期：2002-04-01 00:00:00

abstract：:Recent experimental evidence from a number of preparations indicates that sodium channel inactivation may be intrinsically voltage sensitive. Intrinsically voltage sensitive inactivation should produce a charge movement. Crayfish giant axons provide a unique opportunity to reexamine the slower components of gating cur...

journal_title：Biophysical journal

authors： Swenson RP Jr

更新日期：1983-03-01 00:00:00

abstract：:Membrane domains known as rafts are rich in cholesterol and sphingolipids, and are thought to be thicker than the surrounding membrane. If so, monolayers should elastically deform so as to avoid exposure of hydrophobic surfaces to water at the raft boundary. We calculated the energy of splay and tilt deformations nece...

journal_title：Biophysical journal

authors： Kuzmin PI,Akimov SA,Chizmadzhev YA,Zimmerberg J,Cohen FS

更新日期：2005-02-01 00:00:00

abstract：:A partially depolarized squid axon membrane is assumed to have a quasi-steady state negative resistance, the membrane potential is clamped at one point, and a distribution of potential along the axon is obtained from the cable equation. Nominal experimental values of -2 ohm cm(2) for the membrane and 6 ohm cm(2) for t...

journal_title：Biophysical journal

authors： COLE KS

更新日期：1961-05-01 00:00:00

abstract：:We describe a new method for selective laser killing of bacteria targeted with light-absorbing gold nanoparticles conjugated with specific antibodies. The multifunctional photothermal (PT) microscope/spectrometer provides a real-time assessment of this new therapeutic intervention. In this integrated system, strong la...

journal_title：Biophysical journal

authors： Zharov VP,Mercer KE,Galitovskaya EN,Smeltzer MS

更新日期：2006-01-15 00:00:00

abstract：:Frog skeletal muscle fibers, mechanically skinned under water-saturated silicone oil, swell upon transfer to aqueous relaxing medium (60 mM KCl; 3 mM MgCl(2); 3 mM ATP; 4 mM EGTA; 20 mM Tris maleate; pH = 7.0; ionic strength 0.15 M). Their cross-sectional areas, estimated with an elliptical approximation, increase 2.3...

journal_title：Biophysical journal

authors： Godt RE,Maughan DW

更新日期：1977-08-01 00:00:00

abstract：:The organization of the normal collagen molecule and fibrils is reviewed and the detection, assay, and isolation of a collagenolytic enzyme from amphibian tadpole tissue are described and its possible significance in metamorphosis is discussed ...

journal_title：Biophysical journal

authors： GROSS J

更新日期：1964-01-01 00:00:00

abstract：:We have examined the mechanisms underlying the voltage sensitivity of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors in voltage-clamped outside-out patches and whole cells taken from the nucleus magnocellularis of the chick. Responses to either glutamate or kainate had outwardly rectifying current-volt...

journal_title：Biophysical journal

authors： Raman IM,Trussell LO

更新日期：1995-11-01 00:00:00

abstract：:Molecular dynamics simulations have been applied to the DNA octamer d(GCGCA-GAAC). d(GTTCGCGC), which has an adenine bulge at the center to determine the pathway for interconversion between the stacked and extended forms. These forms are known to be important in the molecular recognition of bulges. From a total of ~35...

journal_title：Biophysical journal

authors： Feig M,Zacharias M,Pettitt BM

更新日期：2001-07-01 00:00:00

abstract：:In this work we tested the hypothesis that transgenic sustained overexpression of IGF-1 prevents age-dependent decreases in charge movement and intracellular Ca(2+) in skeletal muscle fibers. To this end, short flexor digitorum brevis (FDB) muscle fibers from 5-7- and 21-24-month-old FVB (wild-type) and S1S2 (IGF-1 tr...

journal_title：Biophysical journal

authors： Wang ZM,Messi ML,Delbono O

更新日期：2002-03-01 00:00:00

abstract：:We investigated the accessibility to protons and thiol-directed reagents of a cysteine substituted at position 338 in transmembrane segment 6 (TM6) of CFTR to test the hypothesis that T338 resides in the pore. Xenopus oocytes expressing T338C CFTR exhibited pH-dependent changes in gCl and I-V shape that were specific ...

journal_title：Biophysical journal

authors： Liu X,Zhang ZR,Fuller MD,Billingsley J,McCarty NA,Dawson DC

更新日期：2004-12-01 00:00:00

abstract：:Stearoylsphingomyelin (SSM) bilayers containing 0, 22, and 50 mol % cholesterol (Chol) and a pentadecanoyl-stearoylphosphatidylcholine (15SPC) bilayer containing 22 mol % Chol were molecular dynamics simulated at two temperatures (37 degrees C and 60 degrees C). 15SPC is the best PC equivalent of SSM. The Chol effect ...

journal_title：Biophysical journal

authors： Róg T,Pasenkiewicz-Gierula M

更新日期：2006-11-15 00:00:00

abstract：:A simple method of simulating cell kinetics experiments, suitable for use on small generally available computers and capable of any degree of accuracy without increase of store required, is described. No assumption is necessary as to cycle-time distribution; the computer program, after being adjusted to generate perce...

journal_title：Biophysical journal

authors： Eaves G

更新日期：1972-11-01 00:00:00

abstract：:In isolated thick filaments from many types of muscle, the two head domains of each myosin molecule are folded back against the filament backbone in a conformation called the interacting heads motif (IHM) in which actin interaction is inhibited. This conformation is present in resting skeletal muscle, but it is not kn...

journal_title：Biophysical journal

authors： Fusi L,Huang Z,Irving M

更新日期：2015-08-18 00:00:00

abstract：:Five AHCAPs exhibiting a broad-spectrum of antimicrobial activity, were examined with regard to their action in lipid mixtures with two anionic lipids, PG and CL. We find that all of the peptides studied were capable of promoting the formation of crystalline phases of DMPG in mixtures of DMPG and CL, without prior inc...

journal_title：Biophysical journal

authors： Epand RF,Maloy L,Ramamoorthy A,Epand RM

更新日期：2010-06-02 00:00:00

abstract：:Accommodation may be defined as an increase in the threshold of an excitable membrane when the membrane is subjected to a sustained subthreshold depolarizing stimulus. Some excitable membranes show accommodation in response to currents which rise linearly at a very slow rate. In this report we point out a theoretical ...

journal_title：Biophysical journal

authors： Jakobsson E,Guttman R

更新日期：1980-08-01 00:00:00

abstract：:The electrogenic transport of ATP and ADP by the mitochondrial ADP/ATP carrier (AAC) was investigated by recording transient currents with two different techniques for performing concentration jump experiments: 1) the fast fluid injection method: AAC-containing proteoliposomes were adsorbed to a solid supported membra...

journal_title：Biophysical journal

authors： Gropp T,Brustovetsky N,Klingenberg M,Müller V,Fendler K,Bamberg E

更新日期：1999-08-01 00:00:00

abstract：:Slow stretch ramps (velocity: 0.17 fiber lengths s-1) were imposed during fused tetanic contractions of intact muscle fibers of the frog (1.4-3.0 degrees C; sarcomere length: 2.12-2.21 microns). Instantaneous force-extension relations were derived both under isometric conditions and during slow stretch by applying fas...

journal_title：Biophysical journal

authors： Månsson A

更新日期：1989-08-01 00:00:00

abstract：:A microscopic model of passive transverse mass transport of small solutes in the viable epidermal layer of human skin is formulated on the basis of a hexagonal array of cells (i.e., keratinocytes) bounded by 4-nm-thick, anisotropic lipid bilayers and separated by 1-μm layers of extracellular fluid. Gap junctions and t...

journal_title：Biophysical journal

authors： Nitsche JM,Kasting GB

更新日期：2013-05-21 00:00:00

abstract：:We present direct quasielastic neutron scattering measurements, in vivo, of macromolecular dynamics in Escherichia coli. The experiments were performed on a wide range of timescales to cover the large panel of internal and self-diffusion motions. Three major internal processes were extracted at physiological temperatu...

journal_title：Biophysical journal

authors： Jasnin M,Moulin M,Haertlein M,Zaccai G,Tehei M

更新日期：2008-07-01 00:00:00

abstract：:The lateral mobility of the neural cell adhesion molecule (NCAM) was examined using single particle tracking (SPT). Various isoforms of human NCAM, differing in their ectodomain, their membrane anchorage mode, or the size of their cytoplasmic domain, were expressed in National Institutes of Health 3T3 cells and C2C12 ...

journal_title：Biophysical journal

authors： Simson R,Yang B,Moore SE,Doherty P,Walsh FS,Jacobson KA

更新日期：1998-01-01 00:00:00

abstract：:Cell adhesion to the extracellular matrix (ECM) is highly active and plays a crucial role in various physiological functions. The active response of cells to physicochemical cues has been universally discovered in multiple microenvironments. However, the mechanisms to rule these active behaviors of cells are still poo...

journal_title：Biophysical journal

authors： Fang Y,Gong H,Yang R,Lai KWC,Quan M

更新日期：2020-06-02 00:00:00

abstract：:Transcription factors use both protein-DNA and protein-protein interactions to assemble appropriate complexes to regulate gene expression. Although most transcription factors operate as monomers or dimers, a few, including the E26 transformation-specific family repressors Drosophila melanogaster Yan and its human homo...

journal_title：Biophysical journal

authors： Hope CM,Rebay I,Reinitz J

更新日期：2017-01-10 00:00:00

abstract：:The partition coefficients (K(P)) of a series of single-chain and double-chain fluorescent amphiphiles, between solid ordered (P(beta') and L(beta)) and liquid disordered (L(alpha) of the type l(d)) lipid phases coexisting in the same lipid bilayer, was studied using steady-state fluorescence emission anisotropy. The ...

journal_title：Biophysical journal

authors： Mesquita RM,Melo E,Thompson TE,Vaz WL

更新日期：2000-06-01 00:00:00

abstract：:It is widely assumed that the decay of fluorescence in photosynthetic systems can be described as a sum of exponential components and that the amplitude of each component is directly related to the absorption cross-section of the antenna pigments coupled to the fluorescing species. We present exact calculations of exc...

journal_title：Biophysical journal

authors： Jean JM,Chan CK,Fleming GR,Owens TG

更新日期：1989-12-01 00:00:00

abstract：:The small mechanosensitive channel, MscS, is a part of the turgor-driven solute efflux system that protects bacteria from lysis in the event of osmotic downshift. It has been identified in Escherichia coli as a product of the orphan yggB gene, now called mscS (Levina et al., 1999, EMBO J. 18:1730). Here I show that th...

journal_title：Biophysical journal

authors： Sukharev S

更新日期：2002-07-01 00:00:00

abstract：:Individual ion activities were calculated for Ca(2+) in pure solutions of CaCl(2) and in mixtures of CaCl(2) with MgCl(2) and with NaCl, using different conventions to obtain gamma(Ca2+). The results were compared with potentiometric measurements, using calcium specific electrodes. It is found that significant differe...

journal_title：Biophysical journal

authors： Shatkay A

更新日期：1968-08-01 00:00:00

abstract：:The kinetic properties of the three taxonomic A substates of sperm whale carbonmonoxy myoglobin in 75% glycerol/buffer are studied by flash photolysis with monitoring in the infrared stretch bands of bound CO at nu(A0) approximately 1967 cm-1, nu(A1) approximately 1947 cm-1, and nu(A3) approximately 1929 cm-1 between ...

journal_title：Biophysical journal

authors： Johnson JB,Lamb DC,Frauenfelder H,Müller JD,McMahon B,Nienhaus GU,Young RD

更新日期：1996-09-01 00:00:00

abstract：:In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by means of commercially ...

journal_title：Biophysical journal

authors： Greiss F,Deligiannaki M,Jung C,Gaul U,Braun D

更新日期：2016-02-23 00:00:00