How to squeeze a sponge: casein micelles under osmotic stress, a SAXS study.

abstract：:We study the lateral headgroup interactions among phosphatidylcholine (PC) molecules and among phosphatidylethanolamine (PE) molecules in monolayers and extend our previous models. In this paper, we present an extensive set of pressure-area isotherms and surface potential experiments on monolayers of phospholipids ran...

journal_title：Biophysical journal

authors： Mingins J,Stigter D,Dill KA

更新日期：1992-06-01 00:00:00

abstract：:The membrane disruption mechanism of pandinin 1 (pin1), an antimicrobial peptide isolated from the venom of the African scorpion, was studied using 31P, 13C, 1H solid-state and multidimensional solution-state NMR spectroscopy. A high-resolution NMR solution structure of pin1 showed that the two distinct alpha-helical ...

journal_title：Biophysical journal

authors： Nomura K,Ferrat G,Nakajima T,Darbon H,Iwashita T,Corzo G

更新日期：2005-12-01 00:00:00

abstract：:An experimental procedure is described which consists of cutting the canal duct, inserting a micropipette and administering known volumetric displacements to the cupula. The cupula is made visible by dying the endolymph. Known displacements are administered to the cupula, and the time constant of the return to its equ...

journal_title：Biophysical journal

authors： Grant JW,Van Buskirk WC

更新日期：1976-06-01 00:00:00

abstract：:Tet repressor (TetR) is involved in the most abundant mechanism of tetracycline (Tc) resistance of gram-negative bacteria. Raman spectra were measured for the class D TetR protein, for an oligodeoxyribonucleotide with sequence corresponding to operator site O1, and for the TetR:oligonucleotide complex. TetR forms a co...

journal_title：Biophysical journal

authors： Krafft C,Hinrichs W,Orth P,Saenger W,Welfle H

更新日期：1998-01-01 00:00:00

abstract：:Recent measurements have demonstrated enzyme activity at hydrations as low as 3%. This raises the question of whether hydration-induced enzyme flexibility is important for activity. Here, to address this, picosecond dynamic neutron scattering experiments are performed on pig liver esterase powders at 0%, 3%, 12%, and ...

journal_title：Biophysical journal

authors： Kurkal V,Daniel RM,Finney JL,Tehei M,Dunn RV,Smith JC

更新日期：2005-08-01 00:00:00

abstract：:Some new approaches to the kinetic study of the reduction of cytochrome aa3 by cytochrome c are presented. The primary innovations are the use of a spectrometer which can acquire multiwavelength data as fast as every 10 microseconds, and the application of a variety of analytical methods which can utilize simultaneous...

journal_title：Biophysical journal

authors： Hendler RW,Bose SK,Shrager RI

更新日期：1993-09-01 00:00:00

abstract：:The engulfment function of macrophages relies on complex molecular interactions involving both lipids and proteins. In particular, the clearance of apoptotic bodies (efferocytosis) is enabled by externalization on the cell target of phosphatidylserine lipids, which activate receptors on macrophages, suggesting that (l...

journal_title：Biophysical journal

authors： Justice MJ,Petrusca DN,Rogozea AL,Williams JA,Schweitzer KS,Petrache I,Wassall SR,Petrache HI

更新日期：2014-02-04 00:00:00

abstract：:Despite the spontaneity of some in vitro protein-folding reactions, native folding in vivo often requires the participation of barrel-shaped multimeric complexes known as chaperonins. Although it has long been known that chaperonin substrates fold upon sequestration inside the chaperonin barrel, the precise mechanism ...

journal_title：Biophysical journal

authors： England JL,Pande VS

更新日期：2008-10-01 00:00:00

abstract：:The composition and electrolyte concentration of the aqueous bathing environment have important consequences for many biological processes and can profoundly affect the behavior of biomolecules. Nevertheless, because of computational limitations, many molecular simulations of biophysical systems can be performed only ...

journal_title：Biophysical journal

authors： Zavadlav J,Sablić J,Podgornik R,Praprotnik M

更新日期：2018-05-22 00:00:00

abstract：:During inflammation, flowing leukocytes tether to and roll on vascular surfaces through the association and dissociation of selectin/ligand bonds. The interactions of P- and L- selectins with their respective ligands exhibit catch-slip bonds, such that increasing force initially prolongs and then shortens bond lifetim...

journal_title：Biophysical journal

authors： Wayman AM,Chen W,McEver RP,Zhu C

更新日期：2010-08-09 00:00:00

abstract：:A complete description of protein structure and function must include a proper treatment of mechanisms that lead to cooperativity. The helix/coil transition serves as a simple example of a cooperative folding process, commonly described by a nucleation-propagation mechanism. The prevalent view is that coil structure m...

journal_title：Biophysical journal

authors： Vorov OK,Livesay DR,Jacobs DJ

更新日期：2009-12-02 00:00:00

abstract：:Early afterdepolarizations (EADs) are voltage oscillations that occur during the repolarizing phase of the cardiac action potential and cause cardiac arrhythmias in a variety of clinical settings. EADs occur in the setting of reduced repolarization reserve and increased inward-over-outward currents, which intuitively ...

journal_title：Biophysical journal

authors： Chang MG,Chang CY,de Lange E,Xu L,O'Rourke B,Karagueuzian HS,Tung L,Marbán E,Garfinkel A,Weiss JN,Qu Z,Abraham MR

更新日期：2012-06-20 00:00:00

abstract：:The size of a cell is central to many functions, including cellular communication and exchange of materials with the environment. This modeling and experimental study focused on understanding how the size of a cell determines its ability to uptake nanometer-scale extracellular materials from the environment. Several m...

journal_title：Biophysical journal

authors： Khetan J,Shahinuzzaman M,Barua S,Barua D

更新日期：2019-01-22 00:00:00

abstract：:Molecular dynamics (MD) simulations of 5 ns on protein-DNA complexes of catabolite-activator protein (CAP), lambda-repressor, and their corresponding uncomplexed protein and DNA, are reported. These cases represent two extremes of DNA bending, with CAP DNA bent severely and the lambda-operator nearly straight when com...

journal_title：Biophysical journal

authors： Dixit SB,Andrews DQ,Beveridge DL

更新日期：2005-05-01 00:00:00

abstract：:ATP, 2-deoxy ATP (dATP), CTP, and UTP support isometric force and unloaded shortening velocity (Vu) to various extents (Regnier et al., Biophys. J. 74:3044-3058). Vu correlated with the rate of cross-bridge dissociation after the power stroke and the steady-state hydrolysis rate in solution, whereas force was modulate...

journal_title：Biophysical journal

authors： Regnier M,Homsher E

更新日期：1998-06-01 00:00:00

abstract：:In an earlier paper which models the cell-cell (or virus-cell) fusion complex as two partial spherical vesicles joined at a narrow neck (Rubin, R. J., and Yi-der Chen. 1990. Biophys. J. 58:1157-1167), the redistribution by diffusion of lipid-like molecules through the neck between the two fused cell surfaces was studi...

journal_title：Biophysical journal

authors： Chen YD,Rubin RJ,Szabo A

更新日期：1993-07-01 00:00:00

abstract：:Ca2+ regulates ryanodine receptor's (RyR) activity through an activating and an inhibiting Ca2+-binding site located on the cytoplasmic side of the RyR channel. Their altered sensitivity plays an important role in the pathology of malignant hyperthermia and heart failure. We used lanthanide ions (Ln3+) as probes to in...

journal_title：Biophysical journal

authors： Sárközi S,Komáromi I,Jóna I,Almássy J

更新日期：2017-05-23 00:00:00

abstract：:Single actin filaments were analyzed in solutions ranging from dilute (0.2 microgram/ml), where filaments interact only with solvent, to concentrations (4.0 mg/ml) at which F-actin forms a nematic phase. A persistence length of approximately 1.8 microns and an average length of approximately 22 microns (Kaufmann et al...

journal_title：Biophysical journal

authors： Käs J,Strey H,Tang JX,Finger D,Ezzell R,Sackmann E,Janmey PA

更新日期：1996-02-01 00:00:00

abstract：:Pyranine (8-hydroxy pyrene-1,3,6-trisulfonate) is a commonly used photoacid that discharges a proton when excited to its first electronic singlet state. Follow-up of its dissociation kinetics reveals the physicochemical properties of its most immediate environment. At vanishing ionic strength the dye adsorbs to the Es...

journal_title：Biophysical journal

authors： Nachliel E,Pollak N,Huppert D,Gutman M

更新日期：2001-03-01 00:00:00

abstract：:We introduce here a novel Monte Carlo simulation method for studying the interactions of hydrophobic peptides with lipid membranes. Each of the peptide's amino acids is represented as two interaction sites: one corresponding to the backbone alpha-carbon and the other to the side chain, with the membrane represented as...

journal_title：Biophysical journal

authors： Kessel A,Shental-Bechor D,Haliloglu T,Ben-Tal N

更新日期：2003-12-01 00:00:00

abstract：:Lipid droplets (LDs), present in many cell types, are highly dynamic organelles that store neutral lipids, primarily triacylglycerols (TAGs). With the discovery of new LD functions (e.g., in immune response, protein clearage, and occurrence with disease), new methods to study LD chemical composition in situ are necess...

journal_title：Biophysical journal

authors： Paul A,Wang Y,Brännmark C,Kumar S,Bonn M,Parekh SH

更新日期：2019-06-18 00:00:00

abstract：:The interaction with lipids of a synthetic peptide corresponding to the transmembrane domain of influenza hemagglutinin was investigated by means of electron spin resonance. A detailed analysis of the electron spin resonance spectra from spin-labeled phospholipids revealed that the major effect of the peptide on the d...

journal_title：Biophysical journal

authors： Ge M,Freed JH

更新日期：2011-01-05 00:00:00

abstract：:Structure-function studies of the Cys loop family of ionotropic neurotransmitter receptors (GABA, nACh, 5-HT(3), and glycine receptors) have resulted in a six-loop (A-F) model of the agonist-binding site. Key amino acids have been identified in these loops that associate with, and stabilize, bound ligand. The next ste...

journal_title：Biophysical journal

authors： Khatri A,Sedelnikova A,Weiss DS

更新日期：2009-01-01 00:00:00

abstract：:Phospholipids have been treated as dimers on a hexagonal lattice, and a move has been introduced that allows the dimers to move and change their orientation on the lattice. Simulations have been performed in which phospholipid chains have been treated as being either independent or infinitely coupled thermodynamically...

journal_title：Biophysical journal

authors： Jerala R,Almeida PF,Biltonen RL

更新日期：1996-08-01 00:00:00

abstract：:It is now rare to find biological, or genetic investigations that do not rely on the tools, data, and thinking drawn from the genomic sciences. Much of this revolution is powered by contemporary sequencing approaches that readily deliver large, genome-wide data sets that not only provide genetic insights but also uniq...

journal_title：Biophysical journal

authors： Schwartz DC

更新日期：2019-12-03 00:00:00

abstract：:Detection algorithms are widely used for the analysis of single-channel data because they remove the background noise from the measured current signal and reconstruct the noise-free time series. Standard detection algorithms assume channels switching only between zero and full conductance. Many types of channels, howe...

journal_title：Biophysical journal

authors： Draber S,Schultze R

更新日期：1994-10-01 00:00:00

abstract：:Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spec...

journal_title：Biophysical journal

authors： Apte DV,Koutalos Y,McFarlane DK,Dawson MJ,Ebrey TG

更新日期：1989-09-01 00:00:00

abstract：:Wiener kernels have been measured for the light growth response of a number of mutants of Phycomyces which show abnormal phototropism (mad mutants). Representative mutants were chosen from the six complementation groups (madA to madF) associated with the light response pathway. One group, madA, associated with the inp...

journal_title：Biophysical journal

authors： Lipson ED

更新日期：1975-10-01 00:00:00

abstract：:GABA and glycine receptors (GlyRs) are pentameric ligand-gated ion channels that respond to the inhibitory neurotransmitters by opening a chloride-selective central pore lined with five M2 segments homologous to those of alpha(1) GlyR/ ARVG(2')LGIT(6')TVLTMTTQSSGSR. The activity of cyanotriphenylborate (CTB) and picro...

journal_title：Biophysical journal

authors： Zhorov BS,Bregestovski PD

更新日期：2000-04-01 00:00:00

abstract：:2H-nuclear magnetic resonance (NMR) has been used to study the dynamics of amino acid residues in bacteriorhodopsin with results that depend on the method of sample preparation. We show here that in [2H]-leucine-labeled samples the intensity of the isotropic signal varies according to the degree of residual contaminat...

journal_title：Biophysical journal

authors： Herzfeld J,Mulliken CM,Siminovitch DJ,Griffin RG

更新日期：1987-11-01 00:00:00