Effects of lipid interactions on model vesicle engulfment by alveolar macrophages.

abstract：:Raman spectroscopic data indicate that the conformations of the two hydrocarbon chains of dipalmitoyl phosphatidylcholine in aqueous dispersions of the lipid differ signficantly. The compounds 1-palmitoyl, 2-palmitoyl-d31-3-sn-phosphatidylcholine and 1-palmitoyl-d31, 2-palmitoyl-3-sn-phosphatidylcholine were synthesiz...

journal_title：Biophysical journal

authors： Gaber BP,Yager P,Peticolas WL

更新日期：1978-12-01 00:00:00

abstract：:We present a theoretical analysis of intracellular calcium waves propagated by calcium feedback at the inositol 1,4,5-trisphosphate (IP3) receptor. The model includes essential features of calcium excitability, but is still analytically tractable. Formulas are derived for the wave speed, amplitude, and width. The calc...

journal_title：Biophysical journal

authors： Kupferman R,Mitra PP,Hohenberg PC,Wang SS

更新日期：1997-06-01 00:00:00

abstract：:Staphylococcus aureus expresses numerous virulence factors that aid in immune evasion. The four-domain staphylococcal immunoglobulin binding (Sbi) protein interacts with complement component 3 (C3) and its thioester domain (C3d)-containing fragments. Recent structural data suggested two possible modes of binding of Sb...

journal_title：Biophysical journal

authors： Gorham RD Jr,Rodriguez W,Morikis D

更新日期：2014-03-04 00:00:00

abstract：:Diverse microbial communities coordinate group behaviors through signal exchange, such as the exchange of acyl-homoserine lactones (AHLs) by Gram-negative bacteria. Cellular communication is prone to interference by neighboring microbes. One mechanism of interference is signal destruction through the production of an ...

journal_title：Biophysical journal

authors： Silva KP,Chellamuthu P,Boedicker JQ

更新日期：2017-03-14 00:00:00

abstract：:Skeletal muscle contraction is a canonical example of motor-driven force generation. Despite the long history of research in this topic, a mechanistic explanation of the collective myosin force generation is lacking. We present a theoretical model of muscle contraction based on the conformational movements of individu...

journal_title：Biophysical journal

authors： Lan G,Sun SX

更新日期：2005-06-01 00:00:00

abstract：:Structures of truncated versions of the influenza A virus M2 proton channel have been determined recently by x-ray crystallography in the open conformation of the channel, and by NMR in the closed state. The structures differ in the position of the bound inhibitors. The x-ray structure shows a single amantadine molecu...

journal_title：Biophysical journal

authors： Chuang GY,Kozakov D,Brenke R,Beglov D,Guarnieri F,Vajda S

更新日期：2009-11-18 00:00:00

abstract：:We studied the effects of permeant ions on the gating of the large conductance Ca(2+)-activated K+ channel from rat skeletal muscle. Rb+ blockade of inward K+ current caused an increase in the open probability as though Rb+ occupancy of the pore interferes with channel closing. In support of this hypothesis, we direct...

journal_title：Biophysical journal

authors： Demo SD,Yellen G

更新日期：1992-03-01 00:00:00

abstract：:Phosphorescence spectroscopy on mouse myeloma IgA J539 in rigid solution at 77K revealed the type of anomalous short-lived component in the tryptophan decay originally observed with lysozyme (Churchich, J.E., 1966. Biochim. Biophys. Acta. 120:406-412) and seen in a large number of Bence Jones proteins (Longworth, J.W....

journal_title：Biophysical journal

authors： Li Z,Galley WC

更新日期：1989-08-01 00:00:00

abstract：:The thermal stability of a Schizolobium parahyba chymotrypsin inhibitor (SPCI) as a function of pH has been investigated using fluorescence, circular dichroism, and differential scanning calorimetry (DSC). The thermodynamic parameters derived from all methods are remarkably similar and strongly suggest the high stabil...

journal_title：Biophysical journal

authors： Teles RC,Calderon Lde A,Medrano FJ,Barbosa JA,Guimarães BG,Santoro MM,de Freitas SM

更新日期：2005-05-01 00:00:00

abstract：:Measurements were made of exchange rates of oxygen, carbon dioxide, and krypton-85 with blood at 37.5 degrees C. Gas transfer took place across a 1 mil silicone rubber membrane. The blood was in a rotating disk boundary layer flow, and the controlling resistance to transfer was the concentration boundary layer. Measur...

journal_title：Biophysical journal

authors： Spaeth EE,Friedlander SK

更新日期：1967-11-01 00:00:00

abstract：:The ryanodine receptor (RyR) of skeletal muscle contains two functional domains: a carboxyl-terminal hydrophobic domain that forms the putative conduction pore of the calcium release channel, and a large cytoplasmic domain that corresponds to the "foot structure." To understand the contribution of the foot structure t...

journal_title：Biophysical journal

authors： Bhat MB,Zhao J,Hayek S,Freeman EC,Takeshima H,Ma J

更新日期：1997-09-01 00:00:00

abstract：:Measurements of voltage relaxations following brief charge-pulses applied to lipid bilayers have been performed at different hydrostatic pressures in the presence of the neutral carriers cyclo (D-Val-L-Pro-L-Val-D-Pro)3(PV) and valinomycin. From double-exponential relaxations observed in membranes containing PV-K+ com...

journal_title：Biophysical journal

authors： Benz R,Conti F

更新日期：1986-07-01 00:00:00

abstract：:Prions are pathological isoforms of the cellular prion protein that is responsible for transmissible spongiform encephalopathies (TSE). Cellular prion protein interacts with copper, Cu(II), through octarepeat and nonoctarepeat (non-OR) binding sites. The molecular details of Cu(II) coordination within the non-OR regio...

journal_title：Biophysical journal

authors： Salzano G,Brennich M,Mancini G,Tran TH,Legname G,D'Angelo P,Giachin G

更新日期：2020-02-04 00:00:00

abstract：:The influence of mass transport on ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor, was investigated. A one-dimensional computer model for the mass transport of ligand between the bulk solution and the polymer gel and within the gel was employed, and the influe...

journal_title：Biophysical journal

authors： Schuck P

更新日期：1996-03-01 00:00:00

abstract：:Cofilin/ADF, beryllium fluoride complex (BeFx), and phalloidin have opposing effects on actin filament structure and dynamics. Cofilin/ADF decreases the stability of F-actin by enhancing disorder in subdomain 2, and by severing and accelerating the depolymerization of the filament. BeFx and phalloidin stabilize the su...

journal_title：Biophysical journal

authors： Muhlrad A,Ringel I,Pavlov D,Peyser YM,Reisler E

更新日期：2006-12-15 00:00:00

abstract：:It has been repeatedly noted that the change of conformation of the molecules that serve as the ion-selective channels for sodium and potassium conductance in the nerve membrane will be accompanied by a change in the dipole moment of the molecule. This time-dependent change of dipole moment will produce transient curr...

journal_title：Biophysical journal

authors： Strandberg MW

更新日期：1977-09-01 00:00:00

abstract：:We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment. The line FRAP method is developed to complement the disk FRAP method reported before. Although being more subject to the influe...

journal_title：Biophysical journal

authors： Braeckmans K,Remaut K,Vandenbroucke RE,Lucas B,De Smedt SC,Demeester J

更新日期：2007-03-15 00:00:00

abstract：:Many studies of specific protein-nucleic acid binding use short oligonucleotides or restriction fragments, in part to minimize the potential for nonspecific binding of the protein. However, when the specificity ratio is low, multiple nonspecifically bound proteins may occupy the region of DNA corresponding to one spec...

journal_title：Biophysical journal

authors： Tsodikov OV,Holbrook JA,Shkel IA,Record MT Jr

更新日期：2001-10-01 00:00:00

abstract：:Fusion of a vesicle with its target membrane is preceded by tethering or docking. However, the physical mechanism of vesicle-tethering is unknown. To study this mechanism, we used eosinophil secretory granules, which undergo stimulated homotypic fusion events inside the cell during degranulation. Using a dual optical ...

journal_title：Biophysical journal

authors： Valero V,Nevian T,Ho D,Lindau M

更新日期：2008-11-15 00:00:00

abstract：:Phase-resolved monitoring of photoacoustic signals can provide information about the depth profile of a sample. We describe an application of this principle to determine the depth profiles of ligands and antitumor agents in mammalian cells. Measurements of the in-phase and quadrature components of the photoacoustic sp...

journal_title：Biophysical journal

authors： Narayanan K,Chandani S,Ramakrishna T,Rao CM

更新日期：1997-05-01 00:00:00

abstract：:The recent work is surveyed which leads to the suggestions that the conformation of globular proteins in solution corresponds to a dynamic ensemble of rapidly interconverting spatial structures, that clusters of hydrophobic amino acid side chains have an important role in the architecture of protein molecules, and tha...

journal_title：Biophysical journal

authors： Wüthrich K,Wagner G,Richarz R,Braun W

更新日期：1980-10-01 00:00:00

abstract：:Acyl chain dynamics of the trans-unsaturated lipid, dielaidoylphosphatidylcholine (DEPC), were studied by conventional and saturation transfer electron paramagnetic resonance spectroscopy of aqueous dispersions of DEPC spin labeled with lecithins having doxyl groups at positions 5, 10, and 14 on the sn-2 chain. The ge...

journal_title：Biophysical journal

authors： Dalton LA,Miller KW

更新日期：1993-10-01 00:00:00

abstract：:Sedimentation velocity is a powerful tool for the analysis of complex solutions of macromolecules. However, sample turbidity imposes an upper limit to the size of molecular complexes currently amenable to such analysis. Furthermore, the breadth of the particle size distribution, combined with possible variations in th...

journal_title：Biophysical journal

authors： Mächtle W

更新日期：1999-02-01 00:00:00

abstract：:Atomic force microscopy (AFM) can be used to probe the mechanics of molecular recognition between surfaces. In the application known as "chemical force" microscopy (CFM), a chemically modified AFM tip probes a surface through chemical recognition. When modified with a biological ligand or receptor, the AFM tip can dis...

journal_title：Biophysical journal

authors： Mazzola LT,Frank CW,Fodor SP,Mosher C,Lartius R,Henderson E

更新日期：1999-06-01 00:00:00

abstract：:The effects of lysolipids on phospholipid layer curvature and bending elasticity were examined using x-ray diffraction and the osmotic stress method. Lysolipids with two different head groups, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and differing hydrocarbon chains were mixed with the hexagonal-for...

journal_title：Biophysical journal

authors： Fuller N,Rand RP

更新日期：2001-07-01 00:00:00

abstract：:Clostridium difficile (C. diff) is one of the most common and most severe hospital-acquired infections; its consequences range from lengthened hospital stay to outright lethality. C. diff causes cellular damage through the action of two large toxins TcdA and TcdB. Recently, there has been increased effort toward devel...

journal_title：Biophysical journal

authors： Swett RJ,Cisneros GA,Feig AL

更新日期：2013-07-16 00:00:00

abstract：:Myelin basic protein (MBP) is predominantly found in the membranes of the myelin sheath of the central nervous system and is involved in important protein-protein and protein-lipid interactions in vivo and in vitro. Furthermore, divalent transition metal ions, especially Zn(2+) and Cu(2+), seem to directly affect the ...

journal_title：Biophysical journal

authors： Bund T,Boggs JM,Harauz G,Hellmann N,Hinderberger D

更新日期：2010-11-03 00:00:00

abstract：:Bases, nucleosides, nucleotides, and polynucleotides were exposed to chemically generated singlet oxygen to determine whether the species oxidized paralleled those oxidized in photodynamic reactions. In neutral or basic aqueous solution guanine, guanosine, deoxyguanosine, guanylic acid, deoxyguanylic acid, thymine, an...

journal_title：Biophysical journal

authors： Hallett FR,Hallett BP,Snipes W

更新日期：1970-04-01 00:00:00

abstract：:The role of the extracellular Glu side chains of bacteriorhodopsin in the proton transport mechanism has been studied using the single mutants E9Q, E74Q, E194Q, and E204Q; the triple mutant E9Q/E194Q/E204Q; and the quadruple mutant E9Q/E74Q/E194Q/E204Q. Steady-state difference and deconvoluted Fourier transform infrar...

journal_title：Biophysical journal

authors： Lazarova T,Sanz C,Querol E,Padrós E

更新日期：2000-04-01 00:00:00

abstract：:The increasingly widespread use of parametric mathematical models to describe biological systems means that the ability to infer model parameters is of great importance. In this study, we consider parameter inferability in nonlinear ordinary differential equation models that undergo a bifurcation, focusing on a simple...

journal_title：Biophysical journal

authors： Kirk PD,Toni T,Stumpf MP

更新日期：2008-07-01 00:00:00