Abstract:
:Fusion of a vesicle with its target membrane is preceded by tethering or docking. However, the physical mechanism of vesicle-tethering is unknown. To study this mechanism, we used eosinophil secretory granules, which undergo stimulated homotypic fusion events inside the cell during degranulation. Using a dual optical trap system, we observed tether formation between isolated eosinophil secretory granules. The results show that secretory granules interact stochastically with a target membrane forming physical tethers linking the vesicle and target membrane, rather than via interactions with the cytoskeleton. The necessary components are membrane-associated, and the addition of cytosolic components is not required. Tether-lifetime measurements as a function of applied mechanical force revealed at least three kinetically distinct tethered states. The tethered-state lifetimes of isolated eosinophil granules match the residence times of chromaffin granules at the plasma membrane in intact cells, suggesting that the tethering mechanisms reported here may represent the physiological mechanisms of vesicle-tethering in the cell.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Valero V,Nevian T,Ho D,Lindau Mdoi
10.1529/biophysj.108.132670subject
Has Abstractpub_date
2008-11-15 00:00:00pages
4972-8issue
10eissn
0006-3495issn
1542-0086pii
S0006-3495(08)78635-1journal_volume
95pub_type
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