Analytical calculation of intracellular calcium wave characteristics.

abstract：:Structural rearrangements of the myosin upper-50 kD subdomain are thought to play a key role in coordinating actin binding with nucleotide hydrolysis during the myosin ATPase cycle. Such rearrangements could open and close the active site in opposition to the actin-binding cleft, helping explain the opposing affinitie...

journal_title：Biophysical journal

authors： Robertson CI,Gaffney DP 2nd,Chrin LR,Berger CL

更新日期：2005-09-01 00:00:00

abstract：:The characteristic equatorial X-ray pattern from a relaxed vertebrate skeletal muscle changes when the muscle is activated. In particular, there is a simultaneous decrease in the intensity of the first reflection (I10) and increase in the intensity of the second (I11). This observed change is almost reciprocal. When c...

journal_title：Biophysical journal

authors： Lymn RW

更新日期：1978-01-01 00:00:00

abstract：:Dendritic shaft (Zd) and spine (Zsp) input impedances were computed numerically for sites on hippocampal neurons, using a segmental format of cable calculations. The Zsp values for a typical spine appended onto a dendritic shaft averaged less than 2% higher than the Zd values for the adjacent dendritic shaft. Spine sy...

journal_title：Biophysical journal

authors： Turner DA

更新日期：1984-07-01 00:00:00

abstract：:In two-color fluorescence correlation spectroscopy (TCFCS), the fluorescence intensities of two fluorescently-labeled species are cross-correlated over time and can be used to identify static and dynamic interactions. Generally, fluorophore labels are chosen that do not undergo Förster resonance energy transfer (FRET)...

journal_title：Biophysical journal

authors： Hom EF,Verkman AS

更新日期：2002-07-01 00:00:00

abstract：:Normally, isothermal titration calorimetry (ITC) is used to study binding reactions between two different biomolecules. Self-association processes leading to homo-oligomeric complexes have usually not been studied by ITC; instead, methods such as spectroscopy and analytical ultracentrifugation, which only provide affi...

journal_title：Biophysical journal

authors： Luke K,Apiyo D,Wittung-Stafshede P

更新日期：2005-11-01 00:00:00

abstract：:Entropy Sampling Monte Carlo (ESMC) simulations were carried out to study the thermodynamics of the folding transition in the GCN4 leucine zipper (GCN4-lz) in the context of a reduced model. Using the calculated partition functions for the monomer and dimer, and taking into account the equilibrium between the monomer ...

journal_title：Biophysical journal

authors： Mohanty D,Kolinski A,Skolnick J

更新日期：1999-07-01 00:00:00

abstract：:13C alpha chemical shifts and site-specific unfolding curves are reported for 12 sites on a 33-residue, GCN4-like leucine zipper peptide (GCN4-lzK), ranging over most of the chain and sampling most heptad positions. Data were derived from NMR spectra of nine synthetic, isosequential peptides bearing 99% 13C alpha at s...

journal_title：Biophysical journal

authors： Holtzer ME,Lovett EG,d'Avignon DA,Holtzer A

更新日期：1997-08-01 00:00:00

abstract：:We report here a method for the determination of the pKa of histidine in complex or heterogeneous systems amenable to neither solid-state nor solution NMR spectroscopy. Careful synthesis of a fluorenylmethyloxycarbonyl- and trityl-protected, C2-deuterated histidine produces a vibrational-probe-equipped amino acid that...

journal_title：Biophysical journal

authors： Braun GA,Pogostin BH,Pucetaite M,Londergan CH,Åkerfeldt KS

更新日期：2020-11-03 00:00:00

abstract：:Fluorescent probes in biological systems are sensitive to environmental polarity by virtue of their response to the reaction field created by polarization of the dielectric medium. Classically, fluorophore solvatochromism is analyzed in terms of the Lippert equation and later variants, all of which rely upon the origi...

journal_title：Biophysical journal

authors： Marsh D

更新日期：2009-04-08 00:00:00

abstract：:The fusion of a membrane-bounded vesicle with a target membrane is a key step in intracellular trafficking, exocytosis, and drug delivery. Molecular dynamics simulations have been used to study the fusion of small unilamellar vesicles composed of a dipalmitoyl-phosphatidylcholine (DPPC)/palmitic acid 1:2 mixture in at...

journal_title：Biophysical journal

authors： Knecht V,Marrink SJ

更新日期：2007-06-15 00:00:00

abstract：:It has recently been shown that the highly protected segments 24-34 (S2) and 83-93 (S8) of each of the two 99-mers of human immunodeficiency virus type 1 protease play an essential role in the folding of the monomers, giving rise to the so-called (postcritical) folding nucleus ((FN) minimum condensation unit ensuring ...

journal_title：Biophysical journal

authors： Bonomi M,Gervasio FL,Tiana G,Provasi D,Broglia RA,Parrinello M

更新日期：2007-10-15 00:00:00

abstract：:The electrophysiological properties of the cation channel of the purified nicotinic acetylcholine receptor (AChR) reconstituted in planar lipid bilayers were characterized. Single-channel currents were activated by acetylcholine, carbamylcholine and suberyldicholine. The single channel conductance (28 pS in 0.3 M NaCl...

journal_title：Biophysical journal

authors： Montal M,Labarca P,Fredkin DR,Suarez-Isla BA

更新日期：1984-01-01 00:00:00

abstract：:To the best of our knowledge, this is the first report on the structure of product-inhibited mammalian peroxidase. Lactoperoxidase is a heme containing an enzyme that catalyzes the inactivation of a wide range of microorganisms. In the presence of hydrogen peroxide, it preferentially converts thiocyanate ion into a to...

journal_title：Biophysical journal

authors： Singh AK,Singh N,Sharma S,Shin K,Takase M,Kaur P,Srinivasan A,Singh TP

更新日期：2009-01-01 00:00:00

abstract：:The depth of insertion of an antimicrobial peptide, protegrin-1 (PG-1), in lipid bilayers is investigated using solid-state NMR. Paramagnetic Mn(2+) ions bind to the surface of lipid bilayers and induce distance-dependent dipolar relaxation of nuclear spins. By comparing the signal dephasing of the peptide with that o...

journal_title：Biophysical journal

authors： Buffy JJ,Hong T,Yamaguchi S,Waring AJ,Lehrer RI,Hong M

更新日期：2003-10-01 00:00:00

abstract：:The bacterial flagellar motor couples the flow of protons across the cytoplasmic membrane to the rotation of a helical flagellar filament. Using tethered cells, we have measured the stall torque required to block this rotation and compared it with the torque of the running motor over a wide range of values of proton-m...

journal_title：Biophysical journal

authors： Meister M,Berg HC

更新日期：1987-09-01 00:00:00

abstract：:We present the results of a detailed molecular dynamics study of the closed form of the P2X4 receptor. The fluctuations observed in the simulations were compared with the changes that occur in the transition from the closed to the open structure. To get further insight on the opening mechanism, the actual displacement...

journal_title：Biophysical journal

authors： Pierdominici-Sottile G,Moffatt L,Palma J

更新日期：2016-12-20 00:00:00

abstract：:Melittin has been reported to form toroidal pores under certain conditions, but the atomic-resolution structure of these pores is unknown. A 9-μs all-atom molecular-dynamics simulation starting from a closely packed transmembrane melittin tetramer in DMPC shows formation of a toroidal pore after 1 μs. The pore remains...

journal_title：Biophysical journal

authors： Leveritt JM 3rd,Pino-Angeles A,Lazaridis T

更新日期：2015-05-19 00:00:00

abstract：:The genetic map of T4 (and T2) bacteriophage is circular but the DNA molecule that is liberated by phenol extraction is a linear duplex of polynucleotide chains. If the genetic map is related to the physical structure of the DNA molecule, the problem arises as to how a linear molecule can give rise to a circular map. ...

journal_title：Biophysical journal

authors： THOMAS CA Jr,RUBENSTEIN I

更新日期：1964-03-01 00:00:00

abstract：:The hyperpolarization-activated cyclic nucleotide-modulated (HCN) cation channels are opened by membrane hyperpolarization, while their activation is modulated by the binding of cyclic adenosine monophosphate (cAMP) in the cytoplasm. Here we investigate the molecular basis of cAMP channel modulation by performing mole...

journal_title：Biophysical journal

authors： Berrera M,Pantano S,Carloni P

更新日期：2006-05-15 00:00:00

abstract：:In this study we describe the expression and function of the two rat type-1 inositol 1,4,5-trisphosphate receptor (InsP3R) ligand binding domain splice variants (SI+/-/SII+). Receptor protein from COS-1 cells transfected with the type-1 InsP3R expression plasmids (pInsP3R-T1, pInsP3R-T1ALT) or control DNA were incorpo...

journal_title：Biophysical journal

authors： Ramos-Franco J,Caenepeel S,Fill M,Mignery G

更新日期：1998-12-01 00:00:00

abstract：:Many aspects of chromatin biology are influenced by the nuclear compartment in which a locus resides, from transcriptional regulation to DNA repair. Further, the dynamic and variable localization of a particular locus across cell populations and over time makes analysis of a large number of cells critical. As a conseq...

journal_title：Biophysical journal

authors： Zhao Y,Schreiner SM,Koo PK,Colombi P,King MC,Mochrie SG

更新日期：2016-07-12 00:00:00

abstract：:The products of MgATP hydrolysis bind to the nucleotide site of myosin and thus may be expected to inhibit the contraction of muscle fibers. We measured the effects of phosphate and MgADP on the isometric tensions and isotonic contraction velocities of glycerinated rabbit psoas muscle at 10 degrees C. Addition of phos...

journal_title：Biophysical journal

authors： Cooke R,Pate E

更新日期：1985-11-01 00:00:00

abstract：:The near-infrared charge transfer band at 760 nm (band III) has been investigated in deoxy and photodissociated dimeric Scapharca hemoglobin. At 300 K, the 10-ns spectrum of the carbonmonoxy derivative photoproduct is shifted by about 6 nm toward longer wavelengths with respect to the deoxy spectrum, both in buffer an...

journal_title：Biophysical journal

authors： Huang J,Leone M,Boffi A,Friedman JM,Chiancone E

更新日期：1996-06-01 00:00:00

abstract：:Allosteric regulation of protein function is recognized to be widespread throughout biology; however, knowledge of allosteric mechanisms, the molecular changes within a protein that couple one binding site to another, is limited. Although mutagenesis is often used to probe allosteric mechanisms, we consider herein wha...

journal_title：Biophysical journal

authors： Carlson GM,Fenton AW

更新日期：2016-05-10 00:00:00

abstract：:Investigating the behavior of breast cancer cells via reaction kinetics may help unravel the mechanisms that underlie metabolic changes in tumors. However, obtaining human in vivo kinetic data is challenging because of difficulties associated with measuring these parameters. Nondestructive methods of measuring lipid c...

journal_title：Biophysical journal

authors： Hou J,Reid NE,Tromberg BJ,Potma EO

更新日期：2020-07-21 00:00:00

abstract：:We have determined directly the effects of the inhibitory peptide phospholamban (PLB) on the rotational dynamics of the calcium pump (Ca-ATPase) of cardiac sarcoplasmic reticulum (SR). This was accomplished by comparing mouse ventricular SR, which has PLB levels similar to those found in other mammals, with mouse atri...

journal_title：Biophysical journal

authors： Voss JC,Mahaney JE,Jones LR,Thomas DD

更新日期：1995-05-01 00:00:00

abstract：:An equation of state for lipid monolayers at the air-water interface is presented, which takes into account the effects of the conformation and the number and position of double bonds of the hydrocarbon chains. The total Hamiltonian of the monolayer is assumed to consist of three terms. Two of them are calculated exac...

journal_title：Biophysical journal

authors： Feng SS,MacDonald RC

更新日期：1995-08-01 00:00:00

abstract：:Dual-color fluorescence cross-correlation analysis is a powerful tool for probing interactions of different fluorescently labeled molecules in aqueous solution. The concept is the selective observation of coordinated spontaneous fluctuations in two separate detection channels that unambiguously reflect the existence o...

journal_title：Biophysical journal

authors： Heinze KG,Rarbach M,Jahnz M,Schwille P

更新日期：2002-09-01 00:00:00

abstract：:Protein aggregation is linked to more than 30 human pathologies, including Alzheimer's and Parkinson's diseases. Since small oligomers that form at the beginning of the fibrillation process probably are the most toxic elements, therapeutic strategies involving fibril fragmentation could be detrimental. An alternative ...

journal_title：Biophysical journal

authors： Pagano RS,López Medus M,Gómez GE,Couto PM,Labanda MS,Landolfo L,D'Alessio C,Caramelo JJ

更新日期：2014-08-05 00:00:00

abstract：:The nanomechanical response of supported lipid bilayers has been studied by force spectroscopy with atomic force microscopy. We have experimentally proved that the amount of ions present in the measuring system has a strong effect on the force needed to puncture a 1,2-dimyristoyl-sn-glycero-3-phosphocholine bilayer wi...

journal_title：Biophysical journal

authors： Garcia-Manyes S,Oncins G,Sanz F

更新日期：2005-09-01 00:00:00