Potential for modulation of the hydrophobic effect inside chaperonins.

abstract：:This contribution describes measurements of lipid bilayer domain line tension based on two-dimensional thermal undulations of membranes with liquid ordered/liquid disordered phase coexistence and near-critical composition at room temperature. Lateral inhomogeneity of lipid and protein composition is currently a subjec...

journal_title：Biophysical journal

authors： Esposito C,Tian A,Melamed S,Johnson C,Tee SY,Baumgart T

更新日期：2007-11-01 00:00:00

abstract：:The redox-driven proton pump cytochrome c oxidase is that enzymatic machinery of the respiratory chain that transfers electrons from cytochrome c to molecular oxygen and thereby splits molecular oxygen to form water. To investigate the reaction mechanism of cytochrome c oxidase on the single vibrational level, we used...

journal_title：Biophysical journal

authors： Heitbrink D,Sigurdson H,Bolwien C,Brzezinski P,Heberle J

更新日期：2002-01-01 00:00:00

abstract：:We present elastic and inelastic incoherent neutron scattering data from a series of trehalose glasses diluted with glycerol. A strong correlation with recently published protein stability data in the same series of glasses illustrates that the dynamics at Q >or= 0.71 A(-1) and omega > 200 MHz are important to stabili...

journal_title：Biophysical journal

authors： Cicerone MT,Soles CL

更新日期：2004-06-01 00:00:00

abstract：:The membrane disruption mechanism of pandinin 1 (pin1), an antimicrobial peptide isolated from the venom of the African scorpion, was studied using 31P, 13C, 1H solid-state and multidimensional solution-state NMR spectroscopy. A high-resolution NMR solution structure of pin1 showed that the two distinct alpha-helical ...

journal_title：Biophysical journal

authors： Nomura K,Ferrat G,Nakajima T,Darbon H,Iwashita T,Corzo G

更新日期：2005-12-01 00:00:00

abstract：:Laurdan fluorescence, novel spectral fitting, and dynamic light scattering were combined to determine lateral lipid organization in mixed lipid membranes of the oxidized lipid, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), and each of the three bilayer lipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (...

journal_title：Biophysical journal

authors： Ranganathan R,Alshammri I,Peric M

更新日期：2020-04-21 00:00:00

abstract：:A reduction in pH induces the release of iron from transferrin in a process that involves a conformational change in the protein from a closed to an open form. Experimental evidence suggests that there must be changes in the protonation states of certain, as yet not clearly identified, residues in the protein accompan...

journal_title：Biophysical journal

authors： Lee DA,Goodfellow JM

更新日期：1998-06-01 00:00:00

abstract：:We have formulated a kinetic model for the primary steps that occur at the cell membrane during receptor-mediated endocytosis. This model includes the diffusion of receptor molecules, the binding of receptors to coated pits, the loss of coated pits by invagination, and random reinsertion of receptors and coated pits. ...

journal_title：Biophysical journal

authors： Keizer J,Ramirez J,Peacock-López E

更新日期：1985-01-01 00:00:00

abstract：:In a relaxed muscle fiber at low ionic strength, the cross-bridges may well be in states comparable to the one that precedes the cross-bridge power stroke (Schoenberg, M. 1988. Adv. Exp. Med. Biol. 226:189-202). Using electron paramagnetic resonance (EPR) and (saturation transfer) electron paramagnetic resonance (ST-E...

journal_title：Biophysical journal

authors： Fajer PG,Fajer EA,Schoenberg M,Thomas DD

更新日期：1991-09-01 00:00:00

abstract：:We used optical tweezers to analyze the effect of jasplakinolide and cyclodextrin on the force exerted by lamellipodia from developing growth cones (GCs) of isolated dorsal root ganglia (DRG) neurons. We found that 25 nM of jasplakinolide, which is known to inhibit actin filament turnover, reduced both the maximal exe...

journal_title：Biophysical journal

authors： Amin L,Ercolini E,Shahapure R,Migliorini E,Torre V

更新日期：2012-06-06 00:00:00

abstract：:We find evidence for a direct transition of insulin monomers into amyloid fibrils without measurable concentrations of oligomers or protofibrils, suggesting that fibrillogenesis may occur directly from assembly of denaturing insulin monomers rather than by successive transitions through protofibril nuclei. To support ...

journal_title：Biophysical journal

authors： Pease LF 3rd,Sorci M,Guha S,Tsai DH,Zachariah MR,Tarlov MJ,Belfort G

更新日期：2010-12-15 00:00:00

abstract：:We report here a multiprotein blind test of a computer method to predict native protein structures based solely on an all-atom physics-based force field. We use the AMBER 96 potential function with an implicit (GB/SA) model of solvation, combined with replica-exchange molecular-dynamics simulations. Coarse conformatio...

journal_title：Biophysical journal

authors： Shell MS,Ozkan SB,Voelz V,Wu GA,Dill KA

更新日期：2009-02-01 00:00:00

abstract：:Crossover motifs are integral components for designing DNA-based nanostructures and nanomechanical devices due to their enhanced rigidity compared to the normal B-DNA. Although the structural rigidity of the double helix B-DNA has been investigated extensively using both experimental and theoretical tools, to date the...

journal_title：Biophysical journal

authors： Santosh M,Maiti PK

更新日期：2011-09-21 00:00:00

abstract：:We used the nonlinear Poisson-Boltzmann equation to calculate electrostatic potentials in the aqueous phase adjacent to model phospholipid bilayers containing mixtures of zwitterionic lipids (phosphatidylcholine) and acidic lipids (phosphatidylserine or phosphatidylglycerol). The aqueous phase (relative permittivity, ...

journal_title：Biophysical journal

authors： Peitzsch RM,Eisenberg M,Sharp KA,McLaughlin S

更新日期：1995-03-01 00:00:00

abstract：:We investigate the permeability of lipid membranes for fluorescence dyes and ions. We find that permeability reaches a maximum close to the chain melting transition of the membranes. Close to transitions, fluctuations in area and compressibility are high, leading to an increased likelihood of spontaneous lipid pore fo...

journal_title：Biophysical journal

authors： Blicher A,Wodzinska K,Fidorra M,Winterhalter M,Heimburg T

更新日期：2009-06-03 00:00:00

abstract：:A general analytical description of the equilibrium and reaction kinetics of DNA multiplex hybridization has been developed. In this approach, multiplex hybridization is considered to be a competitive multichannel reaction process: a system wherein many species can react both specifically and nonspecifically with one ...

journal_title：Biophysical journal

authors： Horne MT,Fish DJ,Benight AS

更新日期：2006-12-01 00:00:00

abstract：:A simulation of the function of the human heart and heart muscle has been developed in the form of a digital computer code. For a given set of values for the input variables, realistic values of the cardiac output variables are predicted. A detailed discussion of the simulation and some results obtained from its appli...

journal_title：Biophysical journal

authors： Hanna WT

更新日期：1973-07-01 00:00:00

abstract：:The kinetics of redistribution of lipid-like molecules between the membranes of two fused spherical vesicles is studied by solving the time-dependent diffusion equation of the system. The effects on the probe redistribution rate of pore size at the fusion junction and the relative sizes of the vesicles are examined. I...

journal_title：Biophysical journal

authors： Rubin RJ,Chen YD

更新日期：1990-11-01 00:00:00

abstract：:Epithelial injury induces rapid recruitment of antimicrobial leukocytes to the wound site. In zebrafish larvae, activation of the epithelial NADPH oxidase Duox at the wound margin is required early during this response. Before injury, leukocytes are near the vascular region, that is, ∼100-300 μm away from the injury s...

journal_title：Biophysical journal

authors： Jelcic M,Enyedi B,Xavier JB,Niethammer P

更新日期：2017-05-09 00:00:00

abstract：:A simple theoretical analysis shows that specificity of double-stranded DNA (dsDNA) targeting by homopyrimidine peptide nucleic acids (hpyPNAs) is a kinetically controlled phenomenon. Our computations give the optimum conditions for sequence-specific targeting of dsDNA by hpyPNAs. The analysis shows that, in agreement...

journal_title：Biophysical journal

authors： Demidov VV,Yavnilovich MV,Frank-Kamenetskii MD

更新日期：1997-06-01 00:00:00

abstract：:Relations describing threshold fluctuation phenomena in nerves are derived by calculating the approximate response of the Hodgkin-Huxley (HH) axon to electrical noise. We use FitzHugh's reduced phase space approximation and describe the dynamics of a noisy nerve by a two-dimensional brownian motion. The theory predict...

journal_title：Biophysical journal

authors： Lecar H,Nossal R

更新日期：1971-12-01 00:00:00

abstract：:In the plant blue-light sensor phototropin, illumination of the chromophoric LOV domains causes activation of the serine/threonine kinase domain. Flavin mononucleotide (FMN) is a chromophore molecule in the two LOV domains (LOV1 and LOV2), but only LOV2 is responsible for kinase activation. Previous studies reported a...

journal_title：Biophysical journal

authors： Yamamoto A,Iwata T,Sato Y,Matsuoka D,Tokutomi S,Kandori H

更新日期：2009-04-08 00:00:00

abstract：:The thermal transitions of fibrillar collagen are investigated with second-harmonic generation polarization anisotropy microscopy. Second-harmonic generation images and polarization anisotropy profiles of corneal stroma heated in the 35-80°C range are analyzed by means of a theoretical model that is suitable to probe ...

journal_title：Biophysical journal

authors： Matteini P,Cicchi R,Ratto F,Kapsokalyvas D,Rossi F,de Angelis M,Pavone FS,Pini R

更新日期：2012-09-19 00:00:00

abstract：:The S4 transmembrane domain of the family of voltage-gated ion channels is generally thought to be the voltage sensor, whose translocation by an applied electric field produces the gating current. Experiments on hSkMI Na(+) channels and both Shaker and EAG K(+) channels indicate which S4 residues cross the membrane-so...

journal_title：Biophysical journal

authors： Lecar H,Larsson HP,Grabe M

更新日期：2003-11-01 00:00:00

abstract：:We have refined the structure of the DNA Three-Way Junction complex, TWJ-TC, described in the companion paper by quantitative analysis of two 2D NOESY spectra (mixing times 60 and 200 ms) obtained in D2O solution. NOESY crosspeak intensities extracted from these spectra were used in two kinds of refinement procedure: ...

journal_title：Biophysical journal

authors： Ouporov IV,Leontis NB

更新日期：1995-01-01 00:00:00

abstract：:The extracellular space of the brain is the heterogeneous porous medium formed by the spaces between the brain cells. Diffusion in this interstitial space is the mechanism by which glucose and oxygen are delivered to the brain cells from the vascular system. It is also a medium for the transport of certain information...

journal_title：Biophysical journal

authors： Nandigam RK,Kroll DM

更新日期：2007-05-15 00:00:00

abstract：:The mechanism for transport of divalent cations across phospholipid bilayers by the ionophore A23187 was investigated. The intrinsic fluorescence of the ionophore was used in equilibrium and rapid-mixing experiments as an indicator of ionophore environment and complexation with divalent cations. The neutral (protonate...

journal_title：Biophysical journal

authors： Kolber MA,Haynes DH

更新日期：1981-11-01 00:00:00

abstract：:Fluorescent probes in biological systems are sensitive to environmental polarity by virtue of their response to the reaction field created by polarization of the dielectric medium. Classically, fluorophore solvatochromism is analyzed in terms of the Lippert equation and later variants, all of which rely upon the origi...

journal_title：Biophysical journal

authors： Marsh D

更新日期：2009-04-08 00:00:00

abstract：:A major challenge in the study of biomolecular assemblies is to identify reaction coordinates that precisely monitor conformational rearrangements. This is central to the interpretation of single-molecule fluorescence resonance energy transfer measurements, where the observed dynamics depends on the labeling strategy....

journal_title：Biophysical journal

authors： Levi M,Nguyen K,Dukaye L,Whitford PC

更新日期：2017-12-19 00:00:00

abstract：:RNA junctions are common secondary structural elements present in a wide range of RNA species. They play crucial roles in directing the overall folding of RNA molecules as well as in a variety of biological functions. In particular, there has been great interest in the dynamics of RNA junctions, including conformation...

journal_title：Biophysical journal

authors： Jung S,Schlick T

更新日期：2014-01-21 00:00:00

abstract：:A major component of green tea extracts, catechin (-)-Epigallocatechin gallate (EGCg), has been reported to be biologically active and interacting with membranes. A recent study reported drastic effects of EGCg on giant unilamellar vesicles (GUVs). In particular, EGCg above 30 microM caused GUVs to burst. Here we inve...

journal_title：Biophysical journal

authors： Sun Y,Hung WC,Chen FY,Lee CC,Huang HW

更新日期：2009-02-01 00:00:00