Improved resolution of tertiary structure elasticity in muscle protein.

abstract：:Unraveling the conformation of membrane-bound viral fusion peptides is essential for understanding how those peptides destabilize the bilayer topology of lipids that is important for virus-cell membrane fusion. Here, molecular dynamics (MD) simulations were performed to investigate the conformation of the 20 amino aci...

journal_title：Biophysical journal

authors： Huang Q,Chen CL,Herrmann A

更新日期：2004-07-01 00:00:00

abstract：:Flagellin is the subunit of the bacterial filament, the micrometer-long propeller of a bacterial flagellum. The protein is believed to undergo unfolding for transport through the channel of the filament and to refold in a chamber at the end of the channel before being assembled into the growing filament. We report a t...

journal_title：Biophysical journal

authors： Chng CP,Kitao A

更新日期：2008-05-15 00:00:00

abstract：:Internally perfused squid giant axons with intact sodium inactivation gating were prepared for gating current experiments. Gating current records were obtained in sinusoidally driven dynamic steady states and as dynamic transients as functions of the mean membrane potential and the frequency of the command sinusoid. C...

journal_title：Biophysical journal

authors： Fohlmeister JF,Adelman WJ Jr

更新日期：1986-09-01 00:00:00

abstract：:It has been a long belief that release of substances from the cell to the extracellular milieu by exocytosis is completed by diffusion of the substances from secretory vesicles through the fusion pore. Involvement of any mechanical force that may be superposed on the diffusion to enhance the releasing process has not ...

journal_title：Biophysical journal

authors： Tsuboi T,Kikuta T,Sakurai T,Terakawa S

更新日期：2002-07-01 00:00:00

abstract：:A mathematical model is formulated for the development of a population of cells in which the individual members may grow and divide or die. A given cell is characterized by its age and volume, and these parameters are assumed to determine the rate of volume growth and the probability per unit time of division or death...

journal_title：Biophysical journal

authors： Bell GI,Anderson EC

更新日期：1967-07-01 00:00:00

abstract：:Two-photon excitation (TPE) spectra of Fura-2, -4F, -6F, -FF, and Furaptra were characterized using a tunable (750-850 nM) ultra-short pulse laser. Two-photon fluorescence of these dyes was studied in free solution and in the cytosol of isolated rabbit ventricular cardiomyocytes. The TPE spectra of the Ca(2+)-free and...

journal_title：Biophysical journal

authors： Wokosin DL,Loughrey CM,Smith GL

更新日期：2004-03-01 00:00:00

abstract：:We have considered whether lecithin surface films below the gel-liquid crystal transition temperature, Tc, are in unique physical states. In general, below Tc, equilibrium films do not exist when surface pressures, pi, exceed about 0.1 dyn/cm. Since surface pressure-surface area isotherms of lecithin films below Tc al...

journal_title：Biophysical journal

authors： Horn LW,Gershfeld NL

更新日期：1977-06-01 00:00:00

abstract：:The motor head of kinesin carries out microtubule binding, ATP hydrolysis, and force generation. Despite a high level of sequence and structural conservation, subtle variations in subdomains of the motor head determine family-specific properties. In particular, both Kinesin-1 (Kin-1) and Kinesin-5 (Kin-5) walk process...

journal_title：Biophysical journal

authors： Hesse WR,Steiner M,Wohlever ML,Kamm RD,Hwang W,Lang MJ

更新日期：2013-05-07 00:00:00

abstract：:Cell adhesion to the extracellular matrix (ECM) is highly active and plays a crucial role in various physiological functions. The active response of cells to physicochemical cues has been universally discovered in multiple microenvironments. However, the mechanisms to rule these active behaviors of cells are still poo...

journal_title：Biophysical journal

authors： Fang Y,Gong H,Yang R,Lai KWC,Quan M

更新日期：2020-06-02 00:00:00

abstract：:The viral genome and replicative enzymes of the human immunodeficiency virus are encased in a shell consisting of assembled mature capsid protein (CA). The core shell is a stable, effective protective barrier, but is also poised for dissolution on cue to allow transmission of the viral genome into its new host. In thi...

journal_title：Biophysical journal

authors： Ehrlich LS,Liu T,Scarlata S,Chu B,Carter CA

更新日期：2001-07-01 00:00:00

abstract：:The earliest event in exocytosis is the formation of a fusion pore, an aqueous channel that connects the lumen of a secretory granule with the extracellular space. We can observe the formation of individual fusion pores and their subsequent dilation or closure by measuring the changes in the admittance of patch-clampe...

journal_title：Biophysical journal

authors： Oberhauser AF,Monck JR,Fernandez JM

更新日期：1992-03-01 00:00:00

abstract：:Changes in the ultraviolet light scattering from a suspension of purple membrane fragments were detected during the photocycle of bacteriorhodopsin with a cross-correlation method. The scattered light intensity from a suspension of membrane fragments containing the protein bacteriorhodopsin was measured on a logarithm...

journal_title：Biophysical journal

authors： Czégé J,Reinisch L

更新日期：1990-09-01 00:00:00

abstract：:Two different stereoisomers of the dioxolane-linked gramicidin A (gA) channels were individually synthesized (the SS and RR dimers;. Science. 244:813-817). The structural differences between these dimers arise from different chiralities within the dioxolane linker. The SS dimer mimics the helicity and the inter- and i...

journal_title：Biophysical journal

authors： Quigley EP,Quigley P,Crumrine DS,Cukierman S

更新日期：1999-11-01 00:00:00

abstract：:Thermodynamic parameters for the binding of the monovalent cations, Li+, Na+, K+, Rb+, Cs+, NH4+, TI+, and Ag+, to gramicidin A and for the binding of TI+ to gramicidin C, incorporated into lysophosphatidylcholine, have been determined using a combination of TI-205 nuclear magnetic resonance spectroscopy and competiti...

journal_title：Biophysical journal

authors： Hinton JF,Fernandez JQ,Shungu DC,Whaley WL,Koeppe RE 2nd,Millett FS

更新日期：1988-09-01 00:00:00

abstract：:Binding features of heroin in whole blood and in blood fractions were delineated by measuring the selective spin-lattice relaxation rates of heroin protons in physiologic conditions. Interaction with some receptor located in the whole human blood or in the human plasma was detected and the apparent binding constant ca...

journal_title：Biophysical journal

authors： Barni Comparini I,Gaggelli E,Marchettini N,Valensin G

更新日期：1985-08-01 00:00:00

abstract：:Zebrafish (Danio rerio) swim within days of fertilization, powered by muscles of the axial myotomes. Forces generated by these muscles can be measured rapidly in whole, intact larval tails by adapting protocols developed for ex vivo muscle mechanics. But it is not known how well these measurements reflect the function...

journal_title：Biophysical journal

authors： Mead AF,Kennedy GG,Palmer BM,Ebert AM,Warshaw DM

更新日期：2020-08-18 00:00:00

abstract：:There is a steadily growing body of experimental data describing the diffusion of acetylcholine in the neuromuscular junction and the subsequent miniature endplate currents produced at the postsynaptic membrane. To gain further insights into the structural features governing synaptic transmission, we have performed ca...

journal_title：Biophysical journal

authors： Smart JL,McCammon JA

更新日期：1998-10-01 00:00:00

abstract：:Mitochondria often reside in subcellular regions with high metabolic demands. We examined the mechanisms that can govern the relocation of mitochondria to these sites in respiratory neurons. Mitochondria were visualized using tetramethylrhodamineethylester, and their movements were analyzed by applying single-particle...

journal_title：Biophysical journal

authors： Mironov SL

更新日期：2007-04-15 00:00:00

abstract：:Moesin, a protein of the ezrin, radixin, and moesin family, which links the plasma membrane to the cytoskeleton, is involved in multiple physiological and pathological processes, including viral budding and infection. Its interaction with the plasma membrane occurs via a key phosphoinositide, the phosphatidyl(4,5)inos...

journal_title：Biophysical journal

authors： Lubart Q,Vitet H,Dalonneau F,Le Roy A,Kowalski M,Lourdin M,Ebel C,Weidenhaupt M,Picart C

更新日期：2018-01-09 00:00:00

abstract：:Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing, and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent (near) field, and it is a popular contrast generator for surface-selective fluoresc...

journal_title：Biophysical journal

authors： Oheim M,Salomon A,Brunstein M

更新日期：2020-05-19 00:00:00

abstract：:Cartilage stiffness was measured ex vivo at the micrometer and nanometer scales to explore structure-mechanical property relationships at smaller scales than has been done previously. A method was developed to measure the dynamic elastic modulus, |E(*)|, in compression by indentation-type atomic force microscopy (IT A...

journal_title：Biophysical journal

authors： Stolz M,Raiteri R,Daniels AU,VanLandingham MR,Baschong W,Aebi U

更新日期：2004-05-01 00:00:00

abstract：:We investigated self-adhesion between highly negatively charged aggrecan macromolecules extracted from bovine cartilage extracellular matrix by performing atomic force microscopy (AFM) imaging and single-molecule force spectroscopy (SMFS) in saline solutions. By controlling the density of aggrecan molecules on both th...

journal_title：Biophysical journal

authors： Harder A,Walhorn V,Dierks T,Fernàndez-Busquets X,Anselmetti D

更新日期：2010-11-17 00:00:00

abstract：:A recently completed model of Ca concentration and movements in the cardiac cell diadic cleft space predicts that removal or neutralization of inner sarcolemmal (SL) leaflet anionic Ca-binding sites at the sarcolemmal border of this space will greatly diminish Na/Ca exchange-mediated Ca efflux. The present study tests...

journal_title：Biophysical journal

authors： Wang SY,Peskoff A,Langer GA

更新日期：1996-05-01 00:00:00

abstract：:A theory of molecular motors is presented that explains how the energy released in single chemical reactions can generate mechanical motion and force. In the simplest case the fluctuating movements of a motor enzyme are well described by a diffusion process on a two-dimensional potential energy surface, where one dime...

journal_title：Biophysical journal

authors： Keller D,Bustamante C

更新日期：2000-02-01 00:00:00

abstract：:The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic flash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that...

journal_title：Biophysical journal

authors： Gohon Y,Dahmane T,Ruigrok RW,Schuck P,Charvolin D,Rappaport F,Timmins P,Engelman DM,Tribet C,Popot JL,Ebel C

更新日期：2008-05-01 00:00:00

abstract：:Members of the spectrin superfamily of proteins contain different numbers of homologous repeats arranged in tandem. Each of these consists of a three-alpha-helix motif, comprising two similarly and one oppositely directed alpha-helical segment joined by nonhelical linkers of characteristic length. The right-handed alp...

journal_title：Biophysical journal

authors： Parry DA,Dixon TW,Cohen C

更新日期：1992-04-01 00:00:00

abstract：:The spermine-induced DNA condensation is a first-order phase transition. Here, we apply a novel technique fluorescence lifetime correlation spectroscopy to analyze this transition in a greater detail. We show that the method allows for the observation of the condensed and uncondensed molecules simultaneously based sol...

journal_title：Biophysical journal

authors： Humpolícková J,Benda A,Sýkora J,Machán R,Kral T,Gasinska B,Enderlein J,Hof M

更新日期：2008-02-01 00:00:00

abstract：:The Green's function technique is applied to a study of breathing modes in a DNA double helix which contains a region of different base pairs from the rest of the double helix. The calculation is performed on a G-C helix in the B conformation with four consecutive base pairs replaced by A-T. The average stretch in hyd...

journal_title：Biophysical journal

authors： Feng Y,Prohofsky EW

更新日期：1990-03-01 00:00:00

abstract：:In this work we have designed a simple system to investigate empirically the image contrast of tapping-mode atomic force microscopy (TMAFM). We modified the cantilever tips with protein molecules (bovine serum albumin or goat anti-biotin antibody) and used these protein-modified cantilevers to scan poly-L-lysine films...

journal_title：Biophysical journal

authors： You H,Yu L

更新日期：1997-12-01 00:00:00

abstract：:Aggregated Markov models are a widely used tool to model patch clamp data measured from single ion channels. These channels must obey the principle of detailed balance in thermodynamic equilibrium; otherwise, the channel is driven by an external source of energy. We investigate the power of a likelihood ratio test for...

journal_title：Biophysical journal

authors： Wagner M,Timmer J

更新日期：2000-12-01 00:00:00