Estimation of anisotropic optical parameters of tissue in a slab geometry.

abstract：:Actin is ubiquitous globular protein that polymerizes into filaments and forms networks that participate in the force generation of eukaryotic cells. Such forces are used for cell motility, cytokinesis, and tissue remodeling. Among those actin networks, we focus on the actin cortex, a dense branched network beneath th...

journal_title：Biophysical journal

authors： Bussonnier M,Carvalho K,Lemière J,Joanny JF,Sykes C,Betz T

更新日期：2014-08-19 00:00:00

abstract：:Diphtheria toxin (DT) contains separate domains for receptor-specific binding, translocation, and enzymatic activity. After binding to cells, DT is taken up into endosome-like acidic compartments where the translocation domain inserts into the endosomal membrane and releases the catalytic domain into the cytosol. The ...

journal_title：Biophysical journal

authors： Kent MS,Yim H,Murton JK,Satija S,Majewski J,Kuzmenko I

更新日期：2008-03-15 00:00:00

abstract：:Influenza neuraminidase is an important drug target. Glycans are present on neuraminidase and are generally considered to inhibit antibody binding via their glycan shield. In this work, we studied the effect of glycans on the binding kinetics of antiviral drugs to the influenza neuraminidase. We created all-atom in si...

journal_title：Biophysical journal

authors： Seitz C,Casalino L,Konecny R,Huber G,Amaro RE,McCammon JA

更新日期：2020-12-01 00:00:00

abstract：:Prior work has shown that concentration profiles of platelets in flowing whole blood and of platelet-sized beads in flowing blood suspensions can include near-wall excesses. A model to describe this phenomenon was built about a single-component convective diffusion equation. To incorporate redistribution to preferred ...

journal_title：Biophysical journal

authors： Yeh C,Calvez AC,Eckstein EC

更新日期：1994-09-01 00:00:00

abstract：:The unfolding and folding of protein barnase has been extensively investigated in bulk conditions under the effect of denaturant and temperature. These experiments provided information about structural and kinetic features of both the native and the unfolded states of the protein, and debates about the possible existe...

journal_title：Biophysical journal

authors： Alemany A,Rey-Serra B,Frutos S,Cecconi C,Ritort F

更新日期：2016-01-05 00:00:00

abstract：:Myoplasmic free calcium transients were measured with antipyrylazo III in voltage clamped segments of frog skeletal muscle fibers and were used to calculate the rate of release (Rrel) of calcium from the sarcoplasmic reticulum. Intramembrane charge movement was measured for the same pulses in the same fibers. During a...

journal_title：Biophysical journal

authors： Simon BJ,Schneider MF

更新日期：1988-12-01 00:00:00

abstract：:A free radical probe was introduced into single barnacle muscle cells, and its freedom of motion inferred from the spin resonance spectra. The probe reported an average local viscosity of 5-10 cp compared with 1 cp for pure water. From a comparison of the temperature dependence of the probe's tumbling rate in model aq...

journal_title：Biophysical journal

authors： Sachs F,Latorre R

更新日期：1974-04-01 00:00:00

abstract：:In striated muscle thin filament activation is initiated by Ca(2+) binding to troponin C and augmented by strong myosin binding to actin (cross-bridge formation). Several lines of evidence have led us to hypothesize that thin filament properties may limit the level and rate of force development in cardiac muscle at al...

journal_title：Biophysical journal

authors： Regnier M,Martin H,Barsotti RJ,Rivera AJ,Martyn DA,Clemmens E

更新日期：2004-09-01 00:00:00

abstract：:A recent thermodynamic model describes a reversible reaction between cholesterol (C) and phospholipid (P) to form a condensed complex C(nq)P(np). Here q and p are relatively prime integers used to define the stoichiometric composition, and n is a measure of cooperativity. The present study applies this model to the sc...

journal_title：Biophysical journal

authors： Anderson TG,McConnell HM

更新日期：2001-11-01 00:00:00

abstract：:We describe a statistical technique, the joint peristimulus time (PST) scatter diagram, for the analysis of data from simultaneously recorded neurons subjected to repeated stimulation. Distinguishable features in the scatter diagram are related to effects of the stimulus on the observed neurons and to functional relat...

journal_title：Biophysical journal

authors： Gerstein GL,Perkel DH

更新日期：1972-05-01 00:00:00

abstract：:Accommodation may be defined as an increase in the threshold of an excitable membrane when the membrane is subjected to a sustained subthreshold depolarizing stimulus. Some excitable membranes show accommodation in response to currents which rise linearly at a very slow rate. In this report we point out a theoretical ...

journal_title：Biophysical journal

authors： Jakobsson E,Guttman R

更新日期：1980-08-01 00:00:00

abstract：:Influenza A virus M2 protein is known to form acid-activated, proton-selective, amantadine-sensitive channels. We directly measured proton uptake in vesicles containing reconstituted M2 by monitoring external pH after addition of valinomycin to vesicles with 100-fold-diluted external [K(+)]. External pH typically incr...

journal_title：Biophysical journal

authors： Moffat JC,Vijayvergiya V,Gao PF,Cross TA,Woodbury DJ,Busath DD

更新日期：2008-01-15 00:00:00

abstract：:Hypothetical hydroxide and proton migration along the linear water chain in Aquaporin GlpF from Escherichia coli are studied by ab initio Car-Parrinello molecular dynamics simulations. It is found that the protein stabilizes a bipolar single file of water. The single file features a contiguous set of water-water hydro...

journal_title：Biophysical journal

authors： Jensen MØ,Röthlisberger U,Rovira C

更新日期：2005-09-01 00:00:00

abstract：:The freshwater polyp Hydra can regenerate from tissue fragments or random cell aggregates. We show that the axis-defining step ("symmetry breaking") of regeneration requires mechanical inflation-collapse oscillations of the initial cell ball. We present experimental evidence that axis definition is retarded if these o...

journal_title：Biophysical journal

authors： Soriano J,Rüdiger S,Pullarkat P,Ott A

更新日期：2009-02-18 00:00:00

abstract：:Previously, we discussed the calculation of the dipole moments of small proteins using the three-dimensional protein data-base. Our results demonstrate that the calculated dipole moments are in acceptable agreement with measured values. We, however, noted the difficulty of the calculation with larger proteins, in part...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1993-05-01 00:00:00

abstract：:The bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel swimming bacteria. Extensive experimental and theoretical studies exist on the structure, assembly, energy input, power generation, and switching mechanism of the motor. In a previous article, we explained the ge...

journal_title：Biophysical journal

authors： Bai F,Lo CJ,Berry RM,Xing J

更新日期：2009-04-22 00:00:00

abstract：:We propose an improved version of variable-angle total internal reflection fluorescence microscopy (vaTIRFM) adapted to modern TIRF setup. This technique involves the recording of a stack of TIRF images, by gradually increasing the incident angle of the light beam on the sample. A comprehensive theory was developed to...

journal_title：Biophysical journal

authors： Cardoso Dos Santos M,Déturche R,Vézy C,Jaffiol R

更新日期：2016-09-20 00:00:00

abstract：:Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique. Spatially resolved fluorescence decays from single mitochondria or only a very few organelles o...

journal_title：Biophysical journal

authors： Ramadass R,Bereiter-Hahn J

更新日期：2008-10-01 00:00:00

abstract：:Intercellular channels formed of members of the gene family of connexins (Cxs) vary from being substantially cation selective to being anion selective. We took advantage of the ability of Cx46 to function as an unopposed hemichannel to examine the basis of Cx charge selectivity. Previously we showed Cx46 hemichannels ...

journal_title：Biophysical journal

authors： Trexler EB,Bukauskas FF,Kronengold J,Bargiello TA,Verselis VK

更新日期：2000-12-01 00:00:00

abstract：:A variety of amphiphilic helical peptides have been shown to exhibit a transition from adsorbing parallel to a membrane surface at low concentrations to inserting perpendicularly into the membrane at high concentrations. Furthermore, this transition has been correlated to the peptides' cytolytic activities. X-ray lame...

journal_title：Biophysical journal

authors： Wu Y,He K,Ludtke SJ,Huang HW

更新日期：1995-06-01 00:00:00

abstract：:By employing classical molecular dynamics, correlation analysis of coupling between slow and fast dynamical modes, and free energy (umbrella) sampling using classical as well as mixed quantum mechanics molecular mechanics force fields, we uncover a possible pathway for phosphoryl transfer in the self-cleaving reaction...

journal_title：Biophysical journal

authors： Radhakrishnan R

更新日期：2007-10-01 00:00:00

abstract：:We describe a statistical mechanical model for lipid-cholesterol mixtures in the P beta' (ripple) phase of lipid bilayers. The model is a simple extension of an earlier model for the ripple phase in pure lipid bilayers. The extension consists of adding a degree of freedom to allow for the occupation of underlying latt...

journal_title：Biophysical journal

authors： Scott HL,McCullough WS

更新日期：1993-05-01 00:00:00

abstract：:Protein-mediated membrane remodeling is a ubiquitous and critical process for proper cellular function. Inverse Bin/Amphiphysin/Rvs (I-BAR) domains drive local membrane deformation as a precursor to large-scale membrane remodeling. We employ a multiscale approach to provide the molecular mechanism of unusual I-BAR dom...

journal_title：Biophysical journal

authors： Jarin Z,Tsai FC,Davtyan A,Pak AJ,Bassereau P,Voth GA

更新日期：2019-08-06 00:00:00

abstract：:Effects of adducts of [PtCl(NH3)3]Cl or chlorodiethylenetriamineplatinum(II) on DNA stability were studied with emphasis on thermodynamic origins of that stability. Oligodeoxyribonucleotide duplexes (15-bp) containing the single, site-specific monofunctional adduct at G-residues of the central sequences TGT/ACA or 5'-...

journal_title：Biophysical journal

authors： Bursova V,Kasparkova J,Hofr C,Brabec V

更新日期：2005-02-01 00:00:00

abstract：:We perform single-molecule flow experiments using confocal microscopy and a microfluidic device for shear rates up to 20,000 s-1 and present results for the shear-induced unraveling and elongation of tethered von Willebrand factor (VWF) multimers. Further, we employ companion Brownian dynamics simulations to help expl...

journal_title：Biophysical journal

authors： Wang Y,Morabito M,Zhang XF,Webb E 3rd,Oztekin A,Cheng X

更新日期：2019-06-04 00:00:00

abstract：:Here we extend the ability to predict hydrodynamic coefficients and other solution properties of rigid macromolecular structures from atomic-level structures, implemented in the computer program HYDROPRO, to models with lower, residue-level resolution. Whereas in the former case there is one bead per nonhydrogen atom,...

journal_title：Biophysical journal

authors： Ortega A,Amorós D,García de la Torre J

更新日期：2011-08-17 00:00:00

abstract：:Understanding how polypeptides can efficiently and reproducibly attain a self-entangled conformation is a compelling biophysical challenge that might shed new light on our general knowledge of protein folding. Complex lassos, namely self-entangled protein structures characterized by a covalent loop sealed by a cystein...

journal_title：Biophysical journal

authors： Perego C,Potestio R

更新日期：2019-07-23 00:00:00

abstract：:A method for combining calculations of residue pKa's with changes in the position of polar hydrogens has been developed. The Boltzmann distributions of proton positions in hydroxyls and neutral titratable residues are found in the same Monte Carlo sampling procedure that determines the amino acid ionization states at ...

journal_title：Biophysical journal

authors： Alexov EG,Gunner MR

更新日期：1997-05-01 00:00:00

abstract：:The absence of comprehensive measured kinetic values and the observed inconsistency in the available in vitro kinetic data has hindered the formulation of network-scale kinetic models of biochemical reaction networks. To meet this challenge we present an approach to construct a convex space, termed the k-cone, which c...

journal_title：Biophysical journal

authors： Famili I,Mahadevan R,Palsson BO

更新日期：2005-03-01 00:00:00

abstract：:Ribosomes isolated from growing hyphae of Neurospora crassa contain 53 per cent protein and 47 per cent RNA and have a sedimentation coefficient of 81S at 20 degrees C and infinite dilution. These ribosomes are stable at pH 7.4 in the presence of 0.01 M and 0.002 M MgCl(2) but undergo a dissociation into smaller parti...

journal_title：Biophysical journal

authors： STORCK R

更新日期：1963-01-01 00:00:00