Topography of Cells Revealed by Variable-Angle Total Internal Reflection Fluorescence Microscopy.


:We propose an improved version of variable-angle total internal reflection fluorescence microscopy (vaTIRFM) adapted to modern TIRF setup. This technique involves the recording of a stack of TIRF images, by gradually increasing the incident angle of the light beam on the sample. A comprehensive theory was developed to extract the membrane/substrate separation distance from fluorescently labeled cell membranes. A straightforward image processing was then established to compute the topography of cells with a nanometric axial resolution, typically 10-20 nm. To highlight the new opportunities offered by vaTIRFM to quantify adhesion process of motile cells, adhesion of MDA-MB-231 cancer cells on glass substrate coated with fibronectin was examined.


Biophys J


Biophysical journal


Cardoso Dos Santos M,Déturche R,Vézy C,Jaffiol R




Has Abstract


2016-09-20 00:00:00














  • An approach for time-resolved x-ray scattering.

    abstract::Recent biological optical spectroscopic studies have correlated discrete spectroscopic states with biological function in several systems. One of the challenges of molecular biophysics is to correlate structural changes with these spectroscopic states. From small-angle x-ray scattering one can obtain a key structural ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Stamatoff J

    更新日期:1979-05-01 00:00:00

  • Interfacial Binding Sites for Cholesterol on TRP Ion Channels.

    abstract::Transient receptor potential (TRP) channels are members of a large family of ion channels located in membranes rich in cholesterol, some of whose functions are affected by the cholesterol content of the membrane. Here, cholesterol binding to TRPs is studied using a docking procedure that allows the transmembrane surfa...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Lee AG

    更新日期:2019-11-19 00:00:00

  • Two mechanisms by which fluorescent oxonols indicate membrane potential in human red blood cells.

    abstract::Optical potentiometric indicators have been used to monitor the transmembrane electrical potential (Em) of many cells and organelles. A better understanding of the mechanisms of dye response is needed for the design of dyes with improved responses and for unambiguous interpretation of experimental results. This paper ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Pratap PR,Novak TS,Freedman JC

    更新日期:1990-04-01 00:00:00

  • Solid-state NMR studies of a diverged microsomal amino-proximate delta12 desaturase peptide reveal causes of stability in bilayer: tyrosine anchoring and arginine snorkeling.

    abstract::This study reports the solid-state NMR spectroscopic characterization of the amino-proximate transmembrane domain (TM-A) of a diverged microsomal delta12-desaturase (CREP-1) in a phospholipid bilayer. A series of TM-A peptides were synthesized with 2H-labeled side chains (Ala-53, -56, and -63, Leu-62, Val-50), and the...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Gibbons WJ Jr,Karp ES,Cellar NA,Minto RE,Lorigan GA

    更新日期:2006-02-15 00:00:00

  • Oxygen concentration inside a functioning photosynthetic cell.

    abstract::The excess oxygen concentration in the photosynthetic membranes of functioning oxygenic photosynthetic cells was estimated using classical diffusion theory combined with experimental data on oxygen production rates of cyanobacterial cells. The excess oxygen concentration within the plesiomorphic cyanobacterium Gloeoba...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kihara S,Hartzler DA,Savikhin S

    更新日期:2014-05-06 00:00:00

  • Carcinoma and SV40-transfected normal ovarian surface epithelial cell comparison by nonphotochemical hole burning.

    abstract::Results are presented of nonphotochemical-hole-burning experiments on the mitochondrial specific dye rhodamine 800 incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV167 carcinoma cells. This dye is selective for the plasma and inner membranes of the mitochondria, as shown b...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Walsh RJ,Reinot T,Hayes JM,Kalli KR,Hartmann LC,Small GJ

    更新日期:2003-02-01 00:00:00

  • Photoreverse reaction dynamics of octopus rhodopsin.

    abstract::Photoreverse reactions of octopus rhodopsin (Rh) from acid-metarhodopsin (Acid-Meta), which is the final product of the photoreaction of Rh, to Rh were studied by the time-resolved transient absorption and transient grating methods. The time course of the absorption signal showed a rapid change within 500 ns followed ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Inoue K,Tsuda M,Terazima M

    更新日期:2007-05-15 00:00:00

  • Torque and rotation rate of the bacterial flagellar motor.

    abstract::This paper describes an analysis of microscopic models for the coupling between ion flow and rotation of bacterial flagella. In model I it is assumed that intersecting half-channels exist on the rotor and the stator and that the driving ion is constrained to move together with the intersection site. Model II is based ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Läuger P

    更新日期:1988-01-01 00:00:00

  • X-ray scattering from labeled membranes.

    abstract::We present a new method for the determination of structural parameters in biological membranes. Recording the continuous scattering of heavy-atom labeled membranes and applying elementary Fourier methods we obtain the scattering of the heavy-atom distribution alone. The details of this distribution are explored by dev...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Stamatoff J,Bilash T,Ching Y,Eisenberger P

    更新日期:1979-12-01 00:00:00

  • Influence of a natural and a synthetic inhibitor of factor XIIIa on fibrin clot rheology.

    abstract::We investigated the origins of greater clot rigidity associated with FXIIIa-dependent cross-linking. Fibrin clots were examined in which cross-linking was controlled through the use of two inhibitors: a highly specific active-center-directed synthetic inhibitor of FXIIIa, 1,3-dimethyl-4,5-diphenyl-2[2(oxopropyl)thio]i...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ryan EA,Mockros LF,Stern AM,Lorand L

    更新日期:1999-11-01 00:00:00

  • Mechanism for Anaphase B: Evaluation of "Slide-and-Cluster" versus "Slide-and-Flux-or-Elongate" Models.

    abstract::Elongation of the mitotic spindle during anaphase B contributes to chromosome segregation in many cells. Here, we quantitatively test the ability of two models for spindle length control to describe the dynamics of anaphase B spindle elongation using experimental data from Drosophila embryos. In the slide-and-flux-or-...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Brust-Mascher I,Civelekoglu-Scholey G,Scholey JM

    更新日期:2015-04-21 00:00:00

  • Amyloid fibrils of glucagon characterized by high-resolution atomic force microscopy.

    abstract::Glucagon solutions at pH 2.0 were subjected to mechanical agitation at 37 degrees C in the presence of a hydrophobic surface to explore the details of aggregation and fiber formation. High-resolution intermittent-contact atomic force microscopy performed in solution revealed the presence of aggregates after 0.5 h; how...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: De Jong KL,Incledon B,Yip CM,DeFelippis MR

    更新日期:2006-09-01 00:00:00

  • Evidence for intermediate S-states as initial phase in the process of oxygen-evolving complex oxidation.

    abstract::We have analyzed flash-induced period-four damped oscillation of oxygen evolution and chlorophyll fluorescence with the aid of a kinetic model of photosystem II. We have shown that, for simulation of the period-four oscillatory behavior of oxygen evolution, it is essential to consider the so-called intermediate S-stat...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Jablonsky J,Lazar D

    更新日期:2008-04-01 00:00:00

  • Impedance analysis of the organ of corti with magnetically actuated probes.

    abstract::An innovative method is presented to measure the mechanical driving point impedance of biological structures up to at least 40 kHz. The technique employs an atomic force cantilever with a ferromagnetic coating and an external magnetic field to apply a calibrated force to the cantilever. Measurement of the resulting ca...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Scherer MP,Gummer AW

    更新日期:2004-08-01 00:00:00

  • Supercritical Angle Fluorescence Microscopy and Spectroscopy.

    abstract::Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing, and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent (near) field, and it is a popular contrast generator for surface-selective fluoresc...

    journal_title:Biophysical journal

    pub_type: 杂志文章,评审


    authors: Oheim M,Salomon A,Brunstein M

    更新日期:2020-05-19 00:00:00

  • Ion effects on gating of the Ca(2+)-activated K+ channel correlate with occupancy of the pore.

    abstract::We studied the effects of permeant ions on the gating of the large conductance Ca(2+)-activated K+ channel from rat skeletal muscle. Rb+ blockade of inward K+ current caused an increase in the open probability as though Rb+ occupancy of the pore interferes with channel closing. In support of this hypothesis, we direct...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Demo SD,Yellen G

    更新日期:1992-03-01 00:00:00

  • Direct detection of cellular adaptation to local cyclic stretching at the single cell level by atomic force microscopy.

    abstract::The cellular response to external mechanical forces has important effects on numerous biological phenomena. The sequences of molecular events that underlie the observed changes in cellular properties have yet to be elucidated in detail. Here we have detected the responses of a cultured cell against locally applied cyc...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Watanabe-Nakayama T,Machida SI,Harada I,Sekiguchi H,Afrin R,Ikai A

    更新日期:2011-02-02 00:00:00

  • Adaptation in cones. A general model.

    abstract::Three features appear to characterize steady-state light adaptation in vertebrate cone photoreceptors: (a) the shape of the "log intensity-response" curve at different levels of adaptation is the same, the only change with adaptation is in the position of the point on the curve about which the cones operate; (b) at hi...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Dawis SM,Purple RL

    更新日期:1982-08-01 00:00:00

  • Instabilities in the transient response of muscle.

    abstract::We investigate the isometric transient response of muscle using a quantitative stochastic model of the actomyosin cycle based on the swinging lever-arm hypothesis. We first consider a single pair of filaments, and show that when values of parameters such as the lever-arm displacement and the cross-bridge elasticity ar...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Vilfan A,Duke T

    更新日期:2003-08-01 00:00:00

  • Multiwavelength analysis of the kinetics of reduction of cytochrome aa3 by cytochrome c.

    abstract::Some new approaches to the kinetic study of the reduction of cytochrome aa3 by cytochrome c are presented. The primary innovations are the use of a spectrometer which can acquire multiwavelength data as fast as every 10 microseconds, and the application of a variety of analytical methods which can utilize simultaneous...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Hendler RW,Bose SK,Shrager RI

    更新日期:1993-09-01 00:00:00

  • Site-specific dichroism analysis utilizing transmission FTIR.

    abstract::Infrared spectroscopy has long been used to examine the average secondary structure and orientation of membrane proteins. With the recent utilization of site-specific isotope labeling (e.g., peptidic 1-(13)C = (18)O) it is now possible to examine localized properties, rather than global averages. The technique of site...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Arbely E,Kass I,Arkin IT

    更新日期:2003-10-01 00:00:00

  • Activation of skinned trabeculae of the guinea pig induced by laser photolysis of caged ATP.

    abstract::The kinetics of force production in chemically skinned trabeculae from the guinea pig were studied by laser photolysis of caged ATP in the presence of Ca2+. Preincubation of the tissue during rigor with the enzyme apyrase was used to reduce the population of MgADP-bound cross-bridges (Martin and Barsotti, 1994). In un...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Martin H,Barsotti RJ

    更新日期:1994-11-01 00:00:00

  • Calcium-actin waves and oscillations of cellular membranes.

    abstract::We propose a mechanism for the formation of membrane oscillations and traveling waves, which arise due to the coupling between the actin cytoskeleton and the calcium flux through the membrane. In our model, the fluid cell membrane has a mobile but constant population of proteins with a convex spontaneous curvature, wh...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Veksler A,Gov NS

    更新日期:2009-09-16 00:00:00

  • Response of Single Cells to Shock Waves and Numerically Optimized Waveforms for Cancer Therapy.

    abstract::Shock waves are used clinically for breaking kidney stones and treating musculoskeletal indications. The mechanisms by which shock waves interact with tissue are still not well understood. Here, ultra-high-speed imaging was used to visualize the deformation of individual cells embedded in a tissue-mimicking phantom wh...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Li D,Pellegrino A,Hallack A,Petrinic N,Jérusalem A,Cleveland RO

    更新日期:2018-03-27 00:00:00

  • HIV-1 integrase catalytic core: molecular dynamics and simulated fluorescence decays.

    abstract::Two molecular dynamics simulations have been carried out on the HIV-1 integrase catalytic core starting from fully determined crystal structures. During the first one, performed in the absence of divalent cation (6-ns long), the catalytic core took on two main conformations. The conformational transition occurs at app...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Laboulais C,Deprez E,Leh H,Mouscadet JF,Brochon JC,Le Bret M

    更新日期:2001-07-01 00:00:00

  • Characterization of the azide-dependent bacteriorhodopsin-like photocycle of salinarum halorhodopsin.

    abstract::The photocycle of salinarum halorhodopsin was investigated in the presence of azide. The azide binds to the halorhodopsin with 150 mM binding constant in the absence of chloride and with 250 mM binding constant in the presence of 1 M chloride. We demonstrate that the azide-binding site is different from that of chlori...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Lakatos M,Groma GI,Ganea C,Lanyi JK,Váró G

    更新日期:2002-04-01 00:00:00

  • Quantitatively imaging chromosomes by correlated cryo-fluorescence and soft x-ray tomographies.

    abstract::Soft x-ray tomography (SXT) is increasingly being recognized as a valuable method for visualizing and quantifying the ultrastructure of cryopreserved cells. Here, we describe the combination of SXT with cryogenic confocal fluorescence tomography (CFT). This correlative approach allows the incorporation of molecular lo...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Smith EA,McDermott G,Do M,Leung K,Panning B,Le Gros MA,Larabell CA

    更新日期:2014-10-21 00:00:00

  • Coding design of positional information for robust morphogenesis.

    abstract::Robust positioning of cells in a tissue against unavoidable noises is important for achieving normal and reproducible morphogenesis. The position in a tissue is represented by morphogen concentrations, and cells read them to recognize their spatial coordinates. From the engineering viewpoint, these positioning process...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Morishita Y,Iwasa Y

    更新日期:2011-11-16 00:00:00

  • Plastoquinol diffusion in linear photosynthetic electron transport.

    abstract::The diffusion of plastoquinol and its binding to the cytochrome bf complex, which occurs during linear photosynthetic electron transport and is analogous to reaction sequences found in most energy-converting membranes, has been studied in intact thylakoid membranes. The flash-induced electron transfer between the late...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Mitchell R,Spillmann A,Haehnel W

    更新日期:1990-10-01 00:00:00

  • Ratchetaxis in Channels: Entry Point and Local Asymmetry Set Cell Directions in Confinement.

    abstract::Cell motility is essential in a variety of biological phenomena ranging from early development to organ homeostasis and diseases. This phenomenon has mainly been studied and characterized on flat surfaces in vitro, whereas such conditions are rarely observed in vivo. Recently, cell motion in three-dimensional microfab...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Le Maout E,Lo Vecchio S,Kumar Korla P,Jinn-Chyuan Sheu J,Riveline D

    更新日期:2020-10-06 00:00:00