Abstract:
:The processes of cell growth and budding of the yeast cells Saccharomyces cerevisiae, which were gently immobilized on 3% agar and submerged in culture medium, were successfully imaged with an atomic force microscope for 6-7 h. Similar experiments on chemically fixed cells did not detect any appreciable change in their appearance except in a few scannings at the very beginning, indicating that the dissolution of agar and/or scraping of its surface by the scanning tip, if any, did not significantly interfere with the images taken thereafter. The increment in the height of many of the untreated cells, accompanied by their lateral enlargement, was taken as an indication of successful imaging of the growth process of yeast cells, together with an image of a growing daughter cell attached to its mother cell.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Gad M,Ikai Adoi
10.1016/S0006-3495(95)80147-5subject
Has Abstractpub_date
1995-12-01 00:00:00pages
2226-33issue
6eissn
0006-3495issn
1542-0086pii
S0006-3495(95)80147-5journal_volume
69pub_type
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更新日期:2008-11-15 00:00:00
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