Abstract:
:Numerous discussions of the relationship of the thermodynamics of radiation to photosynthesis have been published, but the results are often in disagreement or at best difficult to compare with one another. The recent treatment of maximal photosynthetic efficiencies by Ross and Calvin is here shown to be directly related to the thermodynamic method of Duysens. A smooth connection between the light and dark conditions is derived, the case of polarized light is considered briefly, and a critique of some other thermodynamic treatments is presented.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Knox RSdoi
10.1016/S0006-3495(69)86457-Xsubject
Has Abstractpub_date
1969-11-01 00:00:00pages
1351-62issue
11eissn
0006-3495issn
1542-0086pii
S0006-3495(69)86457-Xjournal_volume
9pub_type
杂志文章abstract::Understanding the intermolecular interaction potential, V(r), of proteins under the influence of temperature, pressure, and salt concentration is essential for understanding protein aggregation, crystallization, and protein phase behavior in general. Here, we report small-angle x-ray scattering studies on dense lysozy...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2012.04.043
更新日期:2012-06-06 00:00:00
abstract::DNA bending is assumed to play a crucial role during recognition of the cAMP-responsive element (CRE) by transcription factors. However, diverging results have been obtained for the bending direction of the unbound double helix. The refined NMR structures present a bend directed toward the minor groove, while biochemi...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(00)76324-7
更新日期:2000-08-01 00:00:00
abstract::In this study we describe the expression and function of the two rat type-1 inositol 1,4,5-trisphosphate receptor (InsP3R) ligand binding domain splice variants (SI+/-/SII+). Receptor protein from COS-1 cells transfected with the type-1 InsP3R expression plasmids (pInsP3R-T1, pInsP3R-T1ALT) or control DNA were incorpo...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(98)77721-5
更新日期:1998-12-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2020.01.028
更新日期:2020-03-24 00:00:00
abstract::Proteins must be stable to accomplish their biological function and to avoid enzymatic degradation. Constitutive proteolysis, however, is the main source of free amino acids used for de novo protein synthesis. In this paper the delicate balance of protein stability and degradability is discussed in the context of func...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(00)76476-9
更新日期:2000-11-01 00:00:00
abstract::The kinetics of aggregation of alpha-synuclein are usually studied by turbidity or Thio-T fluorescence. Here we follow the disappearance of monomers and the formation of early oligomers using fluorescence correlation spectroscopy. Alexa488-labeled A140C-synuclein was used as a fluorescent probe in trace amounts in the...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.12.4290
更新日期:2010-04-07 00:00:00
abstract::We study the migration of chemotactic wild-type Escherichia coli populations in semisolid (soft) agar in the concentration range C = 0.15-0.5% (w/v). For C≲0.35%, expanding bacterial colonies display characteristic chemotactic rings. At C = 0.35%, however, bacteria migrate as broad circular bands rather than sharp rin...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2011.06.023
更新日期:2011-08-03 00:00:00
abstract::We have chemically modified a fraction of the monomers in actin filaments, and then measured the effects on the functional interaction of myosin with unmodified monomers within the same filament. Two modifications were used: (a) covalent attachment of various amounts of myosin subfragment-1 (S1) with the bifunctional ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(93)81057-9
更新日期:1993-07-01 00:00:00
abstract::Membrane domains known as rafts are rich in cholesterol and sphingolipids, and are thought to be thicker than the surrounding membrane. If so, monolayers should elastically deform so as to avoid exposure of hydrophobic surfaces to water at the raft boundary. We calculated the energy of splay and tilt deformations nece...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.048223
更新日期:2005-02-01 00:00:00
abstract::Formation of α-helices is a fundamental process in protein folding and assembly. By studying helix formation in molecular simulations of a series of alanine-based peptides, we obtain the temperature-dependent α-helix propensities of all 20 naturally occurring residues with two recent additive force fields, Amber ff03w...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2012.02.024
更新日期:2012-03-21 00:00:00
abstract::The active amplification of sound-induced vibrations in the cochlea, known to be crucial for auditory sensitivity and frequency selectivity, is not well understood. The outer hair cell (OHC) somatic electromotility is a potential mechanism for such amplification. Its effectiveness in vivo is putatively limited by the ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2011.12.040
更新日期:2012-02-08 00:00:00
abstract::To map the binding sites involved in channel formation, synthetic peptides representing sequences of connexin 32 were tested for their ability to inhibit cell-cell channel formation. Both large peptides representing most of the two presumed extracellular loops of connexin32 and shorter peptides representing subsets of...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(94)80663-0
更新日期:1994-11-01 00:00:00
abstract::Previous studies have demonstrated that the carboxyl terminus of the gap junction protein Cx43 (Cx43CT) can act as an independent, regulatory domain that modulates intercellular communication in response to appropriate chemical stimuli. Here, we have used NMR, chemical cross-linking, and analytical ultracentrifugation...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.103.039230
更新日期:2004-07-01 00:00:00
abstract::Fluorescence contributions from immobile sources present a challenge for fluorescence fluctuation spectroscopy (FFS) because the absence of signal fluctuations from stationary fluorophores leads to a biased analysis. This is especially of concern for cellular FFS studies on proteins that interact with immobile structu...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.107.115642
更新日期:2008-03-15 00:00:00
abstract::The kinetics of conformational changes of P-type ATPases necessary for the occlusion or deocclusion of transported ions are known to be sensitive to the composition of the surrounding membrane, e.g., phospholipid content, mole percentage of cholesterol, and the presence of lipid-bound anions. Research has shown that m...
journal_title:Biophysical journal
pub_type: 杂志文章,评审
doi:10.1016/j.bpj.2015.08.022
更新日期:2015-10-20 00:00:00
abstract::We investigate a temperature-driven recursive division of binary giant unilamellar vesicles (GUVs). During the heating step of the heating-cooling cycle, the spherical mother vesicle deforms to a budded limiting shape using up the excess area produced by the chain melting of the lipids and then splits off into two dau...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2016.02.028
更新日期:2016-04-12 00:00:00
abstract::The pKa values of ionizable groups that lie between the active site region of bacteriorhodopsin (bR) and the extracellular surface of the protein are reported. Glu-204 is found to have an elevated pKa in the resting state of bR, suggesting that it corresponds to the proton-releasing group in bR. Its elevated pKa is pr...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(96)79320-7
更新日期:1996-09-01 00:00:00
abstract::We introduce dual-color time-integrated fluorescence cumulant analysis (TIFCA) to analyze fluorescence fluctuation spectroscopy data. Dual-color TIFCA utilizes the bivariate cumulants of the integrated fluorescent intensity from two detection channels to extract the brightness in each channel, the occupation number, a...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.086181
更新日期:2006-10-01 00:00:00
abstract::Molecular dynamics simulations in simplified models allow one to study the scaling properties of folding times for many proteins together under a controlled setting. We consider three variants of the Go models with different contact potentials and demonstrate scaling described by power laws and no correlation with the...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(03)74867-X
更新日期:2003-01-01 00:00:00
abstract::Proteins are not rigid molecules, but exhibit internal motions on timescales ranging from femto- to milliseconds and beyond. In solution, proteins also experience global translational and rotational motions, sometimes on timescales comparable to those of the internal fluctuations. The possibility that internal and glo...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2011.05.041
更新日期:2011-07-06 00:00:00
abstract::Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280 nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin g...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.105.059550
更新日期:2005-08-01 00:00:00
abstract::We have previously observed that the hydrophobic polyelectrolyte poly(2-ethylacrylic acid) solubilizes lipid membranes in a pH-dependent manner, and we have exploited this phenomenon to prepare lipid vesicles that release their contents in response to pH, light, or glucose (Thomas, J. L., and D. A. Tirrell. Acc. Chem....
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(94)80575-2
更新日期:1994-09-01 00:00:00
abstract::Skeletal muscle contraction is a canonical example of motor-driven force generation. Despite the long history of research in this topic, a mechanistic explanation of the collective myosin force generation is lacking. We present a theoretical model of muscle contraction based on the conformational movements of individu...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.056846
更新日期:2005-06-01 00:00:00
abstract::The crowded environment of cells poses a challenge for rapid protein-protein association. Yet, it has been established that the rates of association are similar in crowded and in dilute solutions. Here we probe the pathway leading to fast association between TEM1 β-lactamase and its inhibitor protein BLIP in crowded s...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2012.08.009
更新日期:2012-09-05 00:00:00
abstract::Starzak and Starzak (1978. IEEE Trans. Biomed. Eng. 25:201-204.) proposed that, in cyclic application of current and voltage-clamps, the fidelity of the match of the output current with the original stimulus could be used to measure the spatial uniformity of voltage in a membrane. However, they failed to find such a m...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(82)84482-2
更新日期:1982-12-01 00:00:00
abstract::To assay cell cycle progression in synchronized culture of yeast we have applied dielectric spectroscopy to its real-time monitoring. The dielectric monitoring is based on the electromagnetic induction method, regarded as a nonelectrode method, which has resolved the problems encountered in measurements with metal ele...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(99)77487-4
更新日期:1999-06-01 00:00:00
abstract::We investigated whether the cysteine residues in Shaker potassium (K+) channels are essential for activation and inactivation gating or for modulation of activation gating by external zinc (Zn2+). Mutants of the Shaker K+ channel were prepared in which all seven cysteine residues were replaced (C-less). These changes ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/s0006-3495(94)80843-4
更新日期:1994-03-01 00:00:00
abstract::Interactions between amyloid-β peptide (Aβ) and the cell membrane include interaction with membrane lipids and binding to membrane receptors, both of which are considered to be the toxicity mechanisms of Aβ. However, it is unclear whether both mechanisms lead to cytotoxicity. Thus, we aimed to analyze these two mechan...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2020.07.035
更新日期:2020-09-15 00:00:00
abstract::Cationic amino acid transporters are highly selective for L-enantiomers such as L-arginine (L-Arg). Because of this stereoselectivity, little is known about the interaction of these transporters with D-isomers. To study whether these compounds can provide information on the molecular mechanism of transport, inward cur...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2010.09.025
更新日期:2010-11-17 00:00:00
abstract::Photoinduced electron transfer in photosystem I (PS I) proceeds from the excited primary electron donor P700 (a chlorophyll a dimer) via the primary acceptor A0 (chlorophyll a) and the secondary acceptor A1 (phylloquinone) to three [4Fe-4S] clusters, Fx, FA, and FB. Prereduction of the iron-sulfur clusters blocks elec...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(98)78023-3
更新日期:1998-06-01 00:00:00