当前位置： SCI文献检索 > BIOPHYSICAL JOURNAL期刊下所有文献 > Function-related regulation of the stability of MHC proteins.

Function-related regulation of the stability of MHC proteins.

Abstract：

:Proteins must be stable to accomplish their biological function and to avoid enzymatic degradation. Constitutive proteolysis, however, is the main source of free amino acids used for de novo protein synthesis. In this paper the delicate balance of protein stability and degradability is discussed in the context of function of major histocompatibility complex (MHC) encoded protein. Classical MHC proteins are single-use peptide transporters that carry proteolytic degradation products to the cell surface for presenting them to T cells. These proteins fulfill their function as long as they bind their dissociable ligand, the peptide. Ligand-free MHC molecules on the cell surface are practically useless for their primary biological function, but may acquire novel activity or become an important source of amino acids when they lose their compact stable structure, which resists proteolytic attacks. We show in this paper that one or more of the stabilization centers responsible for the stability of MHC-peptide complexes is composed of residues of both the protein and the peptide, therefore missing in the ligand-free protein. This arrangement of stabilization centers provides a simple means of regulation; it makes the useful form of the protein stable, whereas the useless form of the same protein is unstable and therefore degradable.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Simon A,Dosztányi Zs,Rajnavölgyi E,Simon I

doi

10.1016/S0006-3495(00)76476-9

subject

Has Abstract

pub_date

2000-11-01 00:00:00

pages

2305-13

issue

5

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(00)76476-9

journal_volume

79

pub_type

杂志文章

相关文献

BIOPHYSICAL JOURNAL文献大全
  • Lipid-Composition-Mediated Forces Can Stabilize Tubular Assemblies of I-BAR Proteins.

    abstract：:Collective action by inverse-Bin/Amphiphysin/Rvs (I-BAR) domains drive micron-scale membrane remodeling. The macroscopic curvature sensing and generation behavior of I-BAR domains is well characterized, and computational models have suggested various mechanisms on simplified membrane systems, but there remain missing ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2020.11.019

    authors： Jarin Z,Pak AJ,Bassereau P,Voth GA

    更新日期：2021-01-05 00:00:00

  • Human PIEZO1: removing inactivation.

    abstract：:PIEZO1 is an inactivating eukaryotic cation-selective mechanosensitive ion channel. Two sites have been located in the channel that when individually mutated lead to xerocytotic anemia by slowing inactivation. By introducing mutations at two sites, one associated with xerocytosis and the other artificial, we were able...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2013.07.019

    authors： Bae C,Gottlieb PA,Sachs F

    更新日期：2013-08-20 00:00:00

  • Single-Molecule Specific Mislocalization of Red Fluorescent Proteins in Live Escherichia coli.

    abstract：:Tagging of individual proteins with genetically encoded fluorescent proteins (FPs) has been used extensively to study localization and interactions in live cells. Recent developments in single-molecule localization microscopy have enabled the dynamic visualization of individual tagged proteins inside living cells. How...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2016.05.047

    authors： Ghodke H,Caldas VE,Punter CM,van Oijen AM,Robinson A

    更新日期：2016-07-12 00:00:00

  • Dendritic signals command firing dynamics in a mathematical model of cerebellar Purkinje cells.

    abstract：:Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.04.056

    authors： Genet S,Sabarly L,Guigon E,Berry H,Delord B

    更新日期：2010-07-21 00:00:00

  • Effects of conformational peptide probe DP4 on bidirectional signaling between DHPR and RyR1 calcium channels in voltage-clamped skeletal muscle fibers.

    abstract：:In skeletal muscle, excitation-contraction coupling involves the activation of dihydropyridine receptors (DHPR) and type-1 ryanodine receptors (RyR1) to produce depolarization-dependent sarcoplasmic reticulum Ca²⁺ release via orthograde signaling. Another form of DHPR-RyR1 communication is retrograde signaling, in whi...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2011.04.012

    authors： Olojo RO,Hernández-Ochoa EO,Ikemoto N,Schneider MF

    更新日期：2011-05-18 00:00:00

  • Pericellular Brush and Mechanics of Guinea Pig Fibroblast Cells Studied with AFM.

    abstract：:The atomic force microscopy (AFM) indentation method combined with the brush model can be used to separate the mechanical response of the cell body from deformation of the pericellular layer surrounding biological cells. Although self-consistency of the brush model to derive the elastic modulus of the cell body has be...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2016.06.005

    authors： Dokukin M,Ablaeva Y,Kalaparthi V,Seluanov A,Gorbunova V,Sokolov I

    更新日期：2016-07-12 00:00:00

  • Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy.

    abstract：:Paxillin is an adaptor molecule involved in the assembly of focal adhesions. Using different fluorescence fluctuation approaches, we established that paxillin-EGFP is dynamic on many timescales within the cell, ranging from milliseconds to seconds. In the cytoplasmic regions, far from adhesions, paxillin is uniformly ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.104984

    authors： Digman MA,Brown CM,Horwitz AR,Mantulin WW,Gratton E

    更新日期：2008-04-01 00:00:00

  • The electromechanics of DNA in a synthetic nanopore.

    abstract：:We have explored the electromechanical properties of DNA on a nanometer-length scale using an electric field to force single molecules through synthetic nanopores in ultrathin silicon nitride membranes. At low electric fields, E < 200 mV/10 nm, we observed that single-stranded DNA can permeate pores with a diameter >/...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.105.070672

    authors： Heng JB,Aksimentiev A,Ho C,Marks P,Grinkova YV,Sligar S,Schulten K,Timp G

    更新日期：2006-02-01 00:00:00

  • Order in phospholipid Langmuir-Blodgett monolayers determined by total internal reflection fluorescence.

    abstract：:Orientational order parameters of two diphenylhexatriene (DPH)-based fluorescent probes, 2-(3-(diphenylhexatrienyl)propanoyl)-1-hexadecanoyl-sn-glycero-3-p hosphocholine (DPHpPC) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH), in dipalmitoylphosphatidylcholine (DPPC) Langmuir-Blodgett monolayers...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(97)78908-2

    authors： Zhai X,Kleijn JM

    更新日期：1997-06-01 00:00:00

  • THE ARRANGEMENTS OF NUCLEOTIDE SEQUENCES IN T2 AND T5 BACTERIOPHAGE DNA MOLECULES.

    abstract：:The genetic map of T4 (and T2) bacteriophage is circular but the DNA molecule that is liberated by phenol extraction is a linear duplex of polynucleotide chains. If the genetic map is related to the physical structure of the DNA molecule, the problem arises as to how a linear molecule can give rise to a circular map. ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/s0006-3495(64)86771-0

    authors： THOMAS CA Jr,RUBENSTEIN I

    更新日期：1964-03-01 00:00:00

  • Correlation between absorption maxima and thermal isomerization rates in bacteriorhodopsin.

    abstract：:The reported rates of thermal 13-cis to all-trans isomerization of the protonated Schiff base of retinal (PSBR) in solution and in bacteriorhodopsin (BR) are shown to be correlated with the red shift in the absorption maximum of the chromophore, though the linear fit is different for BR and for a model PSBR in solutio...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(91)82070-7

    authors： Milder SJ

    更新日期：1991-08-01 00:00:00

  • Thermal memory in self-assembled collagen fibril networks.

    abstract：:Collagen fibrils form extracellular networks that regulate cell functions and provide mechanical strength to tissues. Collagen fibrillogenesis is an entropy-driven process promoted by warming and reversed by cooling. Here, we investigate the influence of noncovalent interactions mediated by the collagen triple helix o...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2013.05.035

    authors： de Wild M,Pomp W,Koenderink GH

    更新日期：2013-07-02 00:00:00

  • Cardiomyopathy Mutation Alters End-to-End Junction of Tropomyosin and Reduces Calcium Sensitivity.

    abstract：:Muscle contraction is governed by tropomyosin (Tpm) shifting azimuthally between three states on F-actin (B-, C-, and M-states) in response to calcium binding to troponin and actomyosin cross-bridge formation. The Tpm coiled coil polymerizes head to tail along the long-pitch helix of F-actin to form continuous superhe...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2019.11.3396

    authors： Sundar S,Rynkiewicz MJ,Ghosh A,Lehman W,Moore JR

    更新日期：2020-01-21 00:00:00

  • Gap junction structures. VIII. Membrane cross-sections.

    abstract：:Profiles of negatively stained gap junctions have been measured by grid sectioning. After normal levels of electron irradiation, the membrane thickness shrinks to about half that of unirradiated controls, but no shrinkage occurs in the hexagonal lattice plane. Even under low irradiation conditions, there is significan...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(88)83152-7

    authors： Sosinsky GE,Jésior JC,Caspar DL,Goodenough DA

    更新日期：1988-05-01 00:00:00

  • Role of a helix B lysine residue in the photoactive site in channelrhodopsins.

    abstract：:In most studied microbial rhodopsins two conserved carboxylic acid residues (the homologs of Asp-85 and Asp-212 in bacteriorhodopsin) and an arginine residue (the homolog of Arg-82) form a complex counterion to the protonated retinylidene Schiff base, and neutralization of the negatively charged carboxylates causes re...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2014.03.002

    authors： Li H,Govorunova EG,Sineshchekov OA,Spudich JL

    更新日期：2014-04-15 00:00:00

  • Binding of anionic lipids to at least three nonannular sites on the potassium channel KcsA is required for channel opening.

    abstract：:In addition to the annular or boundary lipids that surround the transmembrane surface of the potassium channel KcsA from Streptomyces lividans, x-ray crystallographic studies have detected one anionic lipid molecule bound at each protein-protein interface in the homotetrameric structure, at sites referred to as nonann...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.117507

    authors： Marius P,Zagnoni M,Sandison ME,East JM,Morgan H,Lee AG

    更新日期：2008-03-01 00:00:00

  • NMR studies in dodecylphosphocholine of a fragment containing the seventh transmembrane helix of a G-protein-coupled receptor from Saccharomyces cerevisiae.

    abstract：:The structure and dynamics of a large segment of Ste2p, the G-protein-coupled alpha-factor receptor from yeast, were studied in dodecylphosphocholine (DPC) micelles using solution NMR spectroscopy. We investigated the 73-residue peptide EL3-TM7-CT40 consisting of the third extracellular loop 3 (EL3), the seventh trans...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.106.103770

    authors： Neumoin A,Arshava B,Becker J,Zerbe O,Naider F

    更新日期：2007-07-15 00:00:00

  • A dynamic model of smooth muscle contraction.

    abstract：:A dynamic model of smooth muscle contraction is presented and is compared with the mechanical properties of vascular smooth muscle in the rat portal vein. The model is based on the sliding filament theory and the assumption that force is produced by cross-bridges extending from the myosin to the actin filaments. Thus,...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(86)83448-8

    authors： Gestrelius S,Borgström P

    更新日期：1986-07-01 00:00:00

  • Membrane potential can be determined in individual cells from the nernstian distribution of cationic dyes.

    abstract：:The distribution of a selection of cationic fluorescent dyes can be used to measure the membrane potential of individual cells with a microfluorometer. The essential attributes of these dyes include membrane permeability, low membrane binding, spectral properties which are insensitive to environment, and, of course, s...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(88)83158-8

    authors： Ehrenberg B,Montana V,Wei MD,Wuskell JP,Loew LM

    更新日期：1988-05-01 00:00:00

  • Imaging cells and tissues with refractive index radiology.

    abstract：:Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation....

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.103.034991

    authors： Hwu Y,Tsai WL,Chang HM,Yeh HI,Hsu PC,Yang YC,Su YT,Tsai HL,Chow GM,Ho PC,Li SC,Moser HO,Yang P,Seol SK,Kim CC,Je JH,Stefanekova E,Groso A,Margaritondo G

    更新日期：2004-12-01 00:00:00

  • Solid-state NMR studies of a diverged microsomal amino-proximate delta12 desaturase peptide reveal causes of stability in bilayer: tyrosine anchoring and arginine snorkeling.

    abstract：:This study reports the solid-state NMR spectroscopic characterization of the amino-proximate transmembrane domain (TM-A) of a diverged microsomal delta12-desaturase (CREP-1) in a phospholipid bilayer. A series of TM-A peptides were synthesized with 2H-labeled side chains (Ala-53, -56, and -63, Leu-62, Val-50), and the...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.105.067884

    authors： Gibbons WJ Jr,Karp ES,Cellar NA,Minto RE,Lorigan GA

    更新日期：2006-02-15 00:00:00

  • Resonant and localized breathing modes in terminal regions of the DNA double helix.

    abstract：:A Green's function approach is used in constructing a dynamic model of a semi-infinite length of the DNA homopolymer B poly(d) . poly(d). Considerable attention is focused on the hydrogen bond stretching close to the terminus. A melting (or breathing) coordinate (M) is defined as an average over the three linking hydr...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(81)84789-3

    authors： Putnam BF,Van Zandt LL,Prohofsky EW,Mei WN

    更新日期：1981-08-01 00:00:00

  • X-ray diffraction indicates that active cross-bridges bind to actin target zones in insect flight muscle.

    abstract：:We report the first time-resolved study of the two-dimensional x-ray diffraction pattern during active contraction in insect flight muscle (IFM). Activation of demembranated Lethocerus IFM was triggered by 1.5-2.5% step stretches (risetime 10 ms; held for 1.5 s) giving delayed active tension that peaked at 100-200 ms....

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(98)77856-7

    authors： Tregear RT,Edwards RJ,Irving TC,Poole KJ,Reedy MC,Schmitz H,Towns-Andrews E,Reedy MK

    更新日期：1998-03-01 00:00:00

  • Effect of hydrophobic surfactant proteins SP-B and SP-C on phospholipid monolayers. Protein structure studied using 2D IR and beta correlation analysis.

    abstract：:We have applied two-dimensional infrared (2D IR) and betanu correlation spectroscopy to in-situ IR spectroscopy of pulmonary surfactant proteins SP-B and SP-C in lipid-protein monolayers at the air-water interface. For both SP-B and SP-C, a statistical windowed autocorrelation method identified two separate surface pr...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/s0006-3495(02)73973-8

    authors： Shanmukh S,Howell P,Baatz JE,Dluhy RA

    更新日期：2002-10-01 00:00:00

  • Instabilities in the transient response of muscle.

    abstract：:We investigate the isometric transient response of muscle using a quantitative stochastic model of the actomyosin cycle based on the swinging lever-arm hypothesis. We first consider a single pair of filaments, and show that when values of parameters such as the lever-arm displacement and the cross-bridge elasticity ar...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(03)74522-6

    authors： Vilfan A,Duke T

    更新日期：2003-08-01 00:00:00

  • Limitation of cell adhesion by the elasticity of the extracellular matrix.

    abstract：:Cell/matrix adhesions are modulated by cytoskeletal or external stresses and adapt to the mechanical properties of the extracellular matrix. We propose that this mechanosensitivity arises from the activation of a mechanosensor located within the adhesion itself. We show that this mechanism accounts for the observed di...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.105.077115

    authors： Nicolas A,Safran SA

    更新日期：2006-07-01 00:00:00

  • Potassium ion accumulation at the external surface of the nodal membrane in frog myelinated fibers.

    abstract：:Potassium accumulation associated with outward membrane potassium current was investigated experimentally in myelinated fibers and analyzed in terms of two models-three-compartment and diffusion in an unstirred layer. In the myelinated fibers, as in squid giant axons, the three-compartment model satisfactorily describ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(80)85028-4

    authors： Moran N,Palti Y,Levitan E,Stämpfli R

    更新日期：1980-12-01 00:00:00

  • Procedure for testing kinetic models of the photocycle of bacteriorhodopsin.

    abstract：:Given some simple kinetic models of the photocycle of bacteriorhodopsin (bR) and data taken at many wavelengths and under conditions that avoid photoselection and steady-state cycling complications, it is shown how to extract the apparent rate constants and the spectra of the intermediates. Special consideration was g...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(82)84543-8

    authors： Nagle JF,Parodi LA,Lozier RH

    更新日期：1982-05-01 00:00:00

  • Structural rearrangements in the active site of smooth-muscle myosin.

    abstract：:Structural rearrangements of the myosin upper-50 kD subdomain are thought to play a key role in coordinating actin binding with nucleotide hydrolysis during the myosin ATPase cycle. Such rearrangements could open and close the active site in opposition to the actin-binding cleft, helping explain the opposing affinitie...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.105.059840

    authors： Robertson CI,Gaffney DP 2nd,Chrin LR,Berger CL

    更新日期：2005-09-01 00:00:00

  • Molecular dynamics simulations of lipid vesicle fusion in atomic detail.

    abstract：:The fusion of a membrane-bounded vesicle with a target membrane is a key step in intracellular trafficking, exocytosis, and drug delivery. Molecular dynamics simulations have been used to study the fusion of small unilamellar vesicles composed of a dipalmitoyl-phosphatidylcholine (DPPC)/palmitic acid 1:2 mixture in at...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.106.103572

    authors： Knecht V,Marrink SJ

    更新日期：2007-06-15 00:00:00