Order in phospholipid Langmuir-Blodgett monolayers determined by total internal reflection fluorescence.

abstract：:Here we present an efficient implementation of line-scan fluorescence correlation spectroscopy (i.e., one-dimensional spatio-temporal image correlation spectroscopy) using a commercial laser scanning microscope, which allows the accurate measurement of diffusion coefficients and concentrations in biological lipid memb...

journal_title：Biophysical journal

authors： Ries J,Chiantia S,Schwille P

更新日期：2009-03-04 00:00:00

abstract：:We have examined the mechanisms underlying the voltage sensitivity of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors in voltage-clamped outside-out patches and whole cells taken from the nucleus magnocellularis of the chick. Responses to either glutamate or kainate had outwardly rectifying current-volt...

journal_title：Biophysical journal

authors： Raman IM,Trussell LO

更新日期：1995-11-01 00:00:00

abstract：:Interactions between amyloid-β peptide (Aβ) and the cell membrane include interaction with membrane lipids and binding to membrane receptors, both of which are considered to be the toxicity mechanisms of Aβ. However, it is unclear whether both mechanisms lead to cytotoxicity. Thus, we aimed to analyze these two mechan...

journal_title：Biophysical journal

authors： Shi JM,Zhu L,Lan X,Zhao DW,He YJ,Sun ZQ,Wu D,Li HY

更新日期：2020-09-15 00:00:00

abstract：:Cardiac contraction and relaxation dynamics result from a set of simultaneously interacting Ca(2+) regulatory mechanisms. In this study, cardiocyte Ca(2+) dynamics were modeled using a set of six differential equations that were based on theories, equations, and parameters described in previous studies. Among the uniq...

journal_title：Biophysical journal

authors： Snyder SM,Palmer BM,Moore RL

更新日期：2000-07-01 00:00:00

abstract：:Determining the spatial relationship of individual proteins in dense assemblies remains a challenge for superresolution nanoscopy. The organization of aquaporin-4 (AQP4) into large plasma membrane assemblies provides an opportunity to image membrane-bound AQP4 antibodies (AQP4-IgG) and evaluate changes in their spatia...

journal_title：Biophysical journal

authors： Soltys JN,Meyer SA,Schumann H,Gibson EA,Restrepo D,Bennett JL

更新日期：2017-04-25 00:00:00

abstract：:Molecular dynamics simulations of RNA-protein complex between Escherichia coli loop E/helix IV (LE/HeIV) rRNA and L25 protein reveal a qualitative agreement between the experimental and simulated structures. The major groove of LE is a prominent rRNA cation-binding site. Divalent cations rigidify the LE major groove g...

journal_title：Biophysical journal

authors： Réblová K,Spacková N,Koca J,Leontis NB,Sponer J

更新日期：2004-11-01 00:00:00

abstract：:EmrE is a small multidrug resistance transporter that has been well studied as a model for secondary active transport. Because transport requires the protein to convert between at least two states open to opposite sides of the membrane, it is expected that blocking these conformational transitions will prevent transpo...

journal_title：Biophysical journal

authors： Dutta S,Morrison EA,Henzler-Wildman KA

更新日期：2014-08-05 00:00:00

abstract：:The conformation of single laminin molecules adsorbed on synthetic substrates is directly observed making use of the phase magnitude in tapping mode atomic force microscopy (AFM). With AFM, it is not possible to differentiate the proteins on the substrate if use is made of the height signal, since the roughness of the...

journal_title：Biophysical journal

authors： Rodríguez Hernández JC,Salmerón Sánchez M,Soria JM,Gómez Ribelles JL,Monleón Pradas M

更新日期：2007-07-01 00:00:00

abstract：:We have measured light-induced voltage changes (electrogenic events) in photosystem II (PSII) core complexes oriented in phospholipid monolayers. These events are compared to those measured in the functionally and structurally closely related reaction centers from the photosynthetic bacterium Rhodobacter sphaeroides. ...

journal_title：Biophysical journal

authors： Höök F,Brzezinski P

更新日期：1994-06-01 00:00:00

abstract：:Ribosomes isolated from growing hyphae of Neurospora crassa contain 53 per cent protein and 47 per cent RNA and have a sedimentation coefficient of 81S at 20 degrees C and infinite dilution. These ribosomes are stable at pH 7.4 in the presence of 0.01 M and 0.002 M MgCl(2) but undergo a dissociation into smaller parti...

journal_title：Biophysical journal

authors： STORCK R

更新日期：1963-01-01 00:00:00

abstract：:It has been a significant challenge to quantitatively study the dynamic intracellular processes in live cells. These studies are essential for a thorough understanding of the underlying mechanisms regulating the signaling pathways and the transitions between cell cycle stages. Our studies of Cdc20, an important mitoti...

journal_title：Biophysical journal

authors： Wang Z,Shah JV,Berns MW,Cleveland DW

更新日期：2006-07-01 00:00:00

abstract：:We have analyzed repellent signal processing in Escherichia coli by flash photorelease of leucine from photolabile precursors. We found that 1). response amplitudes of free-swimming cell populations increased with leucine jump concentration, with an apparent Hill coefficient of 1.3 and a half-maximal dose of 14.4 micr...

journal_title：Biophysical journal

authors： Khan S,Jain S,Reid GP,Trentham DR

更新日期：2004-06-01 00:00:00

abstract：:Cellular membranes are laterally organized into domains of distinct structures and compositions by the differential interaction affinities between various membrane lipids and proteins. A prominent example of such structures are lipid rafts, which are ordered, tightly packed domains that have been widely implicated in ...

journal_title：Biophysical journal

authors： Lin X,Gorfe AA,Levental I

更新日期：2018-04-24 00:00:00

abstract：:The change in capacity of squid axon membrane during hyper- and depolarizations was investigated in the absence of ionic currents after the membrane was treated with pronase. In the presence of the inactivation process (h parameter), failure to observe the gating current in the frequency domain was attributed to the r...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1978-04-01 00:00:00

abstract：:The antifungal polyene antibiotic amphotericin B forms channels in lipid membranes that are permeable to ions, water, and nonelectrolytes. Anion, cation, and ion pair coordination in the water-filled pore of the "barrel" unit of the channels was studied by molecular dynamics simulations. Unlike the case of the gramici...

journal_title：Biophysical journal

authors： Khutorsky V

更新日期：1996-12-01 00:00:00

abstract：:The potassium selective, inward rectifier current (IK1) is known to be responsible for maintaining the resting membrane potential of quiescent ventricular myocytes. However, the contribution of this current to the different phases of the cardiac action potential has not been adequately established. In the present stud...

journal_title：Biophysical journal

authors： Ibarra J,Morley GE,Delmar M

更新日期：1991-12-01 00:00:00

abstract：:Recent suggestions of nanoscale heat confinement on the surface of synthetic and biogenic magnetic nanoparticles during heating by radio frequency-alternating magnetic fields have generated intense interest because of the potential utility of this phenomenon for noninvasive control of biomolecular and cellular functio...

journal_title：Biophysical journal

authors： Davis HC,Kang S,Lee JH,Shin TH,Putterman H,Cheon J,Shapiro MG

更新日期：2020-03-24 00:00:00

abstract：:Ataxin-1 is a human protein responsible for spinocerebellar ataxia type 1, a hereditary disease associated with protein aggregation and misfolding. Essential for ataxin-1 aggregation is the anomalous expansion of a polyglutamine tract near the protein N-terminus, but the sequence-wise distant AXH domain modulates and ...

journal_title：Biophysical journal

authors： de Chiara C,Rees M,Menon RP,Pauwels K,Lawrence C,Konarev PV,Svergun DI,Martin SR,Chen YW,Pastore A

更新日期：2013-03-19 00:00:00

abstract：:We describe a combined use of experimental and simulation techniques to configure side chains in a coiled coil structure. As already demonstrated in a previous work, x-ray diffraction patterns from hard alpha-keratin fibers in the 5.15 A meridian zone reflect the global configuration of the chi(1) dihedral angle of th...

journal_title：Biophysical journal

authors： Briki F,Doucet J,Etchebest C

更新日期：2002-10-01 00:00:00

abstract：:An experimental method and analysis are introduced which provide direct quantitation of the strength of adhesive contact for large agglutinin-bonded regions between macroscopically smooth membrane capsules (e.g., red blood cells). The approach yields intrinsic properties for separation of adherent regions independent ...

journal_title：Biophysical journal

authors： Berk D,Evans E

更新日期：1991-04-01 00:00:00

abstract：:In the intestinal epithelium, proliferated epithelial cells ascend the crypts and villi and shed at the villus tips into the gut lumen. In this study, we theoretically investigate the roles of the villi on cell turnover. We present a stochastic model that focuses on the duration over which cells migrate the shortest p...

journal_title：Biophysical journal

authors： Kai Y

更新日期：2021-01-13 00:00:00

abstract：:Self-aggregation of isolated plant light-harvesting complexes (LHCs) upon detergent extraction is associated with fluorescence quenching and is used as an in vitro model to study the photophysical processes of nonphotochemical quenching (NPQ). In the NPQ state, in vivo induced under excess solar light conditions, harm...

journal_title：Biophysical journal

authors： Pandit A,Shirzad-Wasei N,Wlodarczyk LM,van Roon H,Boekema EJ,Dekker JP,de Grip WJ

更新日期：2011-11-16 00:00:00

abstract：:A recently completed model of Ca concentration and movements in the cardiac cell diadic cleft space predicts that removal or neutralization of inner sarcolemmal (SL) leaflet anionic Ca-binding sites at the sarcolemmal border of this space will greatly diminish Na/Ca exchange-mediated Ca efflux. The present study tests...

journal_title：Biophysical journal

authors： Wang SY,Peskoff A,Langer GA

更新日期：1996-05-01 00:00:00

abstract：:Fluorescence correlation microscopy (FCM), the combination of fluorescence correlation spectroscopy (FCS) and digital microscopy (Brock and Jovin, 1998. Cell. Mol. Biol. 44:847-856), has been implemented for measuring molecular diffusion and association in living cells with explicit consideration of autocorrelations a...

journal_title：Biophysical journal

authors： Brock R,Hink MA,Jovin TM

更新日期：1998-11-01 00:00:00

abstract：:Coarse-grained molecular dynamics simulations are applied to explore the experimentally observed ability of the liquid-ordered (lo)/liquid-disordered (ld) phase boundary to facilitate viral membrane fusion. Surprisingly, a formed fusion stalk can be both attracted (i.e., stalkophilic) and repelled (i.e., stalkophobic)...

journal_title：Biophysical journal

authors： Risselada HJ

更新日期：2017-06-20 00:00:00

abstract：:In diseases of the brain, the distribution and properties of ion channels display deviations from healthy control subjects. We studied three cases of ion channel alteration related to epileptogenesis. The first case of ion channel alteration represents an enhanced sodium current, the second case addresses the downregu...

journal_title：Biophysical journal

authors： Tigerholm J,Fransén E

更新日期：2011-10-19 00:00:00

abstract：:Several strategies directed toward increasing the participation of minority students in physics and biophysics are presented. Since the number of minority students entering college with an interest in science and mathematics must be increased if we expect to see more students graduating in science, several programs ai...

journal_title：Biophysical journal

authors： Tanaka JC,Gladney LD

更新日期：1993-07-01 00:00:00

abstract：:Guanidinium and acetamidinium, when added to the bathing solution in concentrations of approximately 0.1M, cause brief blocks in the single channel potassium currents from channels formed in planar lipid bilayers by gramicidin A. Single channel lifetimes are not affected indicating that the channel structure is not mo...

journal_title：Biophysical journal

authors： Hemsley G,Busath D

更新日期：1991-04-01 00:00:00

abstract：:The oxygen concentration in tissue can vary on several length scales. The basic scale of variation is determined by capillary spacing. It is this scale that is manifest in the simplest Krogh cylinder model. A second, smaller scale of variation is associated with the consumption of oxygen by mitochondria. This paper gi...

journal_title：Biophysical journal

authors： Clark A Jr,Clark PA

更新日期：1985-12-01 00:00:00

abstract：:Membrane potential of aortic endothelial cells under resting conditions is dominated by inward-rectifier K(+) channels belonging to the Kir 2 family. Regulation of endothelial Kir by membrane cholesterol was studied in bovine aortic endothelial cells by altering the sterol composition of the cell membrane. Our results...

journal_title：Biophysical journal

authors： Romanenko VG,Rothblat GH,Levitan I

更新日期：2002-12-01 00:00:00