Inner sarcolemmal leaflet Ca(2+) binding: its role in cardiac Na/Ca exchange.

abstract：:In permeabilized single fibers of fast (psoas) and slow (soleus) muscle from the rabbit, the effect of pH on isometric myofibrillar ATPase activity and force was studied at 15 degrees C, in the pH range 6.4-7.9. ATPase activity was measured photometrically by enzymatic coupling of the regeneration of ATP to the oxidat...

journal_title：Biophysical journal

authors： Potma EJ,van Graas IA,Stienen GJ

更新日期：1994-12-01 00:00:00

abstract：:Sharing of positive or negative regulators between multiple targets is frequently observed in cellular signaling cascades. For instance, phosphatase sharing between multiple kinases is ubiquitous within the MAPK pathway. Here we investigate how such phosphatase sharing could shape robustness and evolvability of the ph...

journal_title：Biophysical journal

authors： Ghosh B,Sarma U,Sourjik V,Legewie S

更新日期：2018-01-09 00:00:00

abstract：:We investigate lateral organization of lipid domains in vesicles versus supported membranes and monolayers. The lipid mixtures used are predominantly DOPC/DPPC/Chol and DOPC/BSM/Chol, which have been previously shown to produce coexisting liquid phases in vesicles and monolayers. In a monolayer at an air-water interfa...

journal_title：Biophysical journal

authors： Stottrup BL,Veatch SL,Keller SL

更新日期：2004-05-01 00:00:00

abstract：:Single-molecule imaging analysis of chemotactic response in eukaryotic cells has revealed a stochastic nature in the input signals and the signal transduction processes. This leads to a fundamental question about the signaling processes: how does the signaling system operate under stochastic fluctuations or noise? Her...

journal_title：Biophysical journal

authors： Ueda M,Shibata T

更新日期：2007-07-01 00:00:00

abstract：:We report a simple method to obtain stable asymmetric giant unilamellar vesicles (GUVs). Fluorescence correlation spectroscopy was used to quantitatively characterize vesicle properties. After brain sphingomyelin (bSM) was exchanged into dioleoylphosphatidylcholine (DOPC) GUVs, lateral diffusion in the bSM-containing ...

journal_title：Biophysical journal

authors： Chiantia S,Schwille P,Klymchenko AS,London E

更新日期：2011-01-05 00:00:00

abstract：:Mechanosensitive ion channels (MSCs) which could provide for fast osmoregulatory responses in bacteria, remain unidentified as molecular entities. MSCs from Escherichia coli (strain AW740) were examined using the patch-clamp technique, either (a) in giant spheroplasts, (b) after reconstitution by fusing native membran...

journal_title：Biophysical journal

authors： Sukharev SI,Martinac B,Arshavsky VY,Kung C

更新日期：1993-07-01 00:00:00

abstract：:Experiments have shown that the ability of the HIV-1 virus to infect cells can be greatly diminished by deactivation of the N-terminal (fusion) peptide of its glycoprotein gp41. Deactivation can be achieved by the deletion of several amino acid residues, or replacement of a hydrophobic residue with a polar residue, to...

journal_title：Biophysical journal

authors： Maddox MW,Longo ML

更新日期：2002-12-01 00:00:00

abstract：:We have characterized the fluorescence properties of 6-dodecanoyl-2-dimethylamine-naphthalene (LAURDAN) in pure interfaces formed by sphingomyelin and 10 chemically related glycosphingolipids (GSLs).1 The GSLs contain neutral and anionic carbohydrate residues in their oligosaccharide chain. These systems were studied ...

journal_title：Biophysical journal

authors： Bagatolli LA,Gratton E,Fidelio GD

更新日期：1998-07-01 00:00:00

abstract：:VP16, a protein encoded by herpes simplex virus, has a well-characterized 78 amino acid acidic activation domain. When tethered to DNA, tandem repeats of an eight amino acid motif taken from this region stimulate the transcription of a nearby gene. This work addresses how these minimal activation motifs interact with ...

journal_title：Biophysical journal

authors： Bamdad C

更新日期：1998-10-01 00:00:00

abstract：:Atomic force microscopy (AFM) is nowadays frequently applied to determine interaction forces between biological molecules. Starting with the detection of the first discrete unbinding forces between ligands and receptors by AFM only several years ago, measurements have become more and more quantitative. At the same tim...

journal_title：Biophysical journal

authors： Willemsen OH,Snel MM,Cambi A,Greve J,De Grooth BG,Figdor CG

更新日期：2000-12-01 00:00:00

abstract：:Chimeric antigen receptors (CARs) have recently been approved for the treatment of hematological malignancies, but our lack of understanding of the basic mechanisms that activate these proteins has made it difficult to optimize and control CAR-based therapies. In this study, we use phosphoproteomic mass spectrometry a...

journal_title：Biophysical journal

authors： Rohrs JA,Zheng D,Graham NA,Wang P,Finley SD

更新日期：2018-09-18 00:00:00

abstract：:Endothelin-1 (ET-1) mediates physiological responses via endothelin A (ET(A)) and B (ET(B)) receptors, which may form homo- and heterodimers with unknown function. Here, we investigated ET-receptor dimerization using fluorescence resonance energy transfer (FRET) between receptors tagged with CFP (donor) and receptors ...

journal_title：Biophysical journal

authors： Evans NJ,Walker JW

更新日期：2008-07-01 00:00:00

abstract：:Ion channel behavior can depend strongly on temperature, with faster kinetics at physiological temperatures leading to considerable changes in currents relative to room temperature. These temperature-dependent changes in voltage-dependent ion channel kinetics (rates of opening, closing, inactivating, and recovery) are...

journal_title：Biophysical journal

authors： Lei CL,Clerx M,Beattie KA,Melgari D,Hancox JC,Gavaghan DJ,Polonchuk L,Wang K,Mirams GR

更新日期：2019-12-17 00:00:00

abstract：:The influence of electrostatic multipole moments up to hexadecapole on the dynamics of photodissociated carbon monoxide (CO) in myoglobin is investigated. The CO electrostatic potential is expressed as an expansion into atomic multipole moments of increasing order up to octopole which are obtained from a distributed m...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2008-04-01 00:00:00

abstract：:We investigate the permeability of lipid membranes for fluorescence dyes and ions. We find that permeability reaches a maximum close to the chain melting transition of the membranes. Close to transitions, fluctuations in area and compressibility are high, leading to an increased likelihood of spontaneous lipid pore fo...

journal_title：Biophysical journal

authors： Blicher A,Wodzinska K,Fidorra M,Winterhalter M,Heimburg T

更新日期：2009-06-03 00:00:00

abstract：:We have used identified neurons from the abdominal ganglion of the mollusc Aplysia to construct and analyze two circuits in vitro. Each of these circuits was capable of producing two patterns of persistent activity; that is, they had bistable output states. The output could be switched between the stable states by a b...

journal_title：Biophysical journal

authors： Kleinfeld D,Raccuia-Behling F,Chiel HJ

更新日期：1990-04-01 00:00:00

abstract：:An analytical electromechanical model of a spherical cell exposed to an alternating electric field was used to calculate shear stress generated in the cellular membrane. Shape deformation of Neurospora crassa (slime) spheroplasts was measured. Statistical analysis permitted empirical evaluation of creep of the cellula...

journal_title：Biophysical journal

authors： Poznański J,Pawłowski P,Fikus M

更新日期：1992-03-01 00:00:00

abstract：:Viscoelastometric measurements of DNA from gamma-irradiated bacteria were used to identify the induction of double-strand breaks ( DSBs ) in the chromosome of Escherichia coli. It is shown by means of inhibitors of repair endonucleases and different repair mutants that most DSBs in DNA of E. coli, gamma-irradiated in ...

journal_title：Biophysical journal

authors： Bresler SE,Noskin LA,Suslov AV

更新日期：1984-04-01 00:00:00

abstract：:A new microscopic technique is demonstrated that combines attributes from both near-field scanning optical microscopy (NSOM) and fluorescence resonance energy transfer (FRET). The method relies on attaching the acceptor dye of a FRET pair to the end of a near-field fiber optic probe. Light exiting the NSOM probe, whic...

journal_title：Biophysical journal

authors： Vickery SA,Dunn RC

更新日期：1999-04-01 00:00:00

abstract：:The continuum and single jump treatments of ion transport through black lipid membranes predict experimentally distinguishable results, even when the same mechanistic assumptions are made and the same potential-distance profile is used. On the basis of steady-state current-voltage curves for nonactin-mediated transpor...

journal_title：Biophysical journal

authors： van Dijk C,de Levie R

更新日期：1985-07-01 00:00:00

abstract：:It has been shown in previous studies that cell poration (i.e., reversible permeabilization of cell membrane) and cell fusion can be induced by applying a pulse (or pulses) of high-intensity DC (direct current) electric field. Recently we suggested that such electro-poration or electro-fusion can also be accomplished ...

journal_title：Biophysical journal

authors： Chang DC

更新日期：1989-10-01 00:00:00

abstract：:Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized wit...

journal_title：Biophysical journal

authors： Thallmair S,Vainikka PA,Marrink SJ

更新日期：2019-04-23 00:00:00

abstract：:Paxillin is an adaptor molecule involved in the assembly of focal adhesions. Using different fluorescence fluctuation approaches, we established that paxillin-EGFP is dynamic on many timescales within the cell, ranging from milliseconds to seconds. In the cytoplasmic regions, far from adhesions, paxillin is uniformly ...

journal_title：Biophysical journal

authors： Digman MA,Brown CM,Horwitz AR,Mantulin WW,Gratton E

更新日期：2008-04-01 00:00:00

abstract：:The α-helical (AH) domain of the hepatitis C virus nonstructural protein NS5A, anchored at the cytoplasmic leaflet of the endoplasmic reticulum, plays a role in viral replication. However, the peptides derived from this domain also exhibit remarkably broad-spectrum virocidal activity, raising questions about their mod...

journal_title：Biophysical journal

authors： Hanson JM,Gettel DL,Tabaei SR,Jackman J,Kim MC,Sasaki DY,Groves JT,Liedberg B,Cho NJ,Parikh AN

更新日期：2016-01-05 00:00:00

abstract：:By employing classical molecular dynamics, correlation analysis of coupling between slow and fast dynamical modes, and free energy (umbrella) sampling using classical as well as mixed quantum mechanics molecular mechanics force fields, we uncover a possible pathway for phosphoryl transfer in the self-cleaving reaction...

journal_title：Biophysical journal

authors： Radhakrishnan R

更新日期：2007-10-01 00:00:00

abstract：:Orientation angles of five emitting dipoles of chlorophyll a in thylakoids were estimated from low temperature fluorescence polarization ratio spectra of magnetically oriented chloroplasts. A simple expression is given also for the evaluation of data from linear dichroism measurements. It is shown that the Qy dipoles ...

journal_title：Biophysical journal

authors： Garab GI,Kiss JG,Mustárdy LA,Michel-Villaz M

更新日期：1981-06-01 00:00:00

abstract：:The prediction of absolute ligand-receptor binding affinities is essential in a wide range of biophysical queries, from the study of protein-protein interactions to structure-based drug design. End-point free energy methods, such as the Molecular Mechanics Poisson-Boltzmann Surface Area (MM/PBSA) model, have received ...

journal_title：Biophysical journal

authors： Swanson JM,Henchman RH,McCammon JA

更新日期：2004-01-01 00:00:00

abstract：:The switch 1 region of myosin forms a lid over the nucleotide phosphates as part of a structure known as the phosphate-tube. The homologous region in kinesin-family motors is more open, not interacting with the nucleotide. We used molecular dynamics (MD) simulations to examine a possible displacement of switch 1 of th...

journal_title：Biophysical journal

authors： Minehardt TJ,Cooke R,Pate E,Kollman PA

更新日期：2001-03-01 00:00:00

abstract：:In the absence of atomic structures of high-density lipoproteins in their lipid-bound states, many molecular models have been produced based on experimental data. Using molecular dynamics, we show that a recently proposed "solar-flares" model of discoidal high-density lipoprotein is implausible. Our simulations show a...

journal_title：Biophysical journal

authors： Shih AY,Sligar SG,Schulten K

更新日期：2008-06-01 00:00:00

abstract：:Impact loading of articular cartilage causes extensive chondrocyte death. Cell membranes have a limited elastic range of 3-4% strain but are protected from direct stretch during physiological loading by their membrane reservoir, an intricate pattern of membrane folds. Using a finite-element model, we suggested previou...

journal_title：Biophysical journal

authors： Moo EK,Amrein M,Epstein M,Duvall M,Abu Osman NA,Pingguan-Murphy B,Herzog W

更新日期：2013-10-01 00:00:00