Endothelin receptor dimers evaluated by FRET, ligand binding, and calcium mobilization.

abstract：:Proteins fold and function in cellular environments that are crowded with other macromolecules. As a consequence of excluded volume effects, compact folded states of proteins should be indirectly stabilized due to destabilization of extended unfolded conformations. Here, we assess the role of excluded volume in terms ...

journal_title：Biophysical journal

authors： Mikaelsson T,Adén J,Johansson LB,Wittung-Stafshede P

更新日期：2013-02-05 00:00:00

abstract：:The general theory (Levitt, D. G. 1990. Biophys. J. 59:271-277) is applied to a model channel that resembles the acetylcholine receptor channel (ACH). The model incorporates the known features of the ACH geometry and fixed charge locations. The channel has a wide mouth facing the outer solution, tapering to a narrow r...

journal_title：Biophysical journal

authors： Levitt DG

更新日期：1991-02-01 00:00:00

abstract：:The p7 protein from hepatitis C virus is critical for the assembly and secretion of infectious virus, making it an attractive drug target. It is thought to be a viroporin with a demonstrated ion channel activity when reconstituted into planar lipid bilayers. Electron microscopy experiments suggest that p7 oligomers co...

journal_title：Biophysical journal

authors： Chew CF,Vijayan R,Chang J,Zitzmann N,Biggin PC

更新日期：2009-01-01 00:00:00

abstract：:Gaining information about receptor profiles on cells, and subsequently finding the most efficient ligands for these signaling receptors, remain challenging tasks in stem cell and cancer research as well as drug development. We introduce a live-cell method with great potential in both screening for surface receptors an...

journal_title：Biophysical journal

authors： Wienken U,Gaub HE

更新日期：2013-12-17 00:00:00

abstract：:Intracellular transport of cargos along microtubules is often complicated by the topology of the underlying filament network. The fundamental building blocks for this complex arrangement are filament intersections. The navigation of cargos across microtubule intersections remains poorly understood. Here, we demonstrat...

journal_title：Biophysical journal

authors： Osunbayo O,Butterfield J,Bergman J,Mershon L,Rodionov V,Vershinin M

更新日期：2015-03-24 00:00:00

abstract：:The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho- and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (Lo or lipid...

journal_title：Biophysical journal

authors： Abbandonato G,Storti B,Tonazzini I,Stöckl M,Subramaniam V,Montis C,Nifosì R,Cecchini M,Signore G,Bizzarri R

更新日期：2019-02-05 00:00:00

abstract：:We propose a general scheme for measuring the attraction between mechanically frustrated semiflexible fibers by measuring their thermal fluctuations and shape. We apply this analysis to a system of sickle hemoglobin (HbS) fibers that laterally attract one another. These fibers appear to "zip" together before reaching ...

journal_title：Biophysical journal

authors： Jones CW,Wang JC,Briehl RW,Turner MS

更新日期：2005-04-01 00:00:00

abstract：:A Ca(2+) spark arises when a cluster of sarcoplasmic reticulum (SR) channels (ryanodine receptors or RyRs) opens to release calcium in a locally regenerative manner. Normally triggered by Ca(2+) influx across the sarcolemmal or transverse tubule membrane neighboring the cluster, the Ca(2+) spark has been shown to be t...

journal_title：Biophysical journal

authors： Sobie EA,Dilly KW,dos Santos Cruz J,Lederer WJ,Jafri MS

更新日期：2002-07-01 00:00:00

abstract：:Two-dimensional electronic chirality-induced signals of excitons in the photosynthetic Fenna-Matthews-Olson complex from two species of green sulfur bacteria (Chlorobium tepidum and Prosthecochloris aestuarii) are compared. The spectra are predicted to provide sensitive probes of local protein environment of the const...

journal_title：Biophysical journal

authors： Voronine DV,Abramavicius D,Mukamel S

更新日期：2008-11-15 00:00:00

abstract：:The results of a 3-ns molecular dynamics simulation of the dodecamer duplex d(TATGGATCCATA)(2) recognized by the BamHI endonuclease are presented here. The DNA has been simulated as a flexible molecule using an AMBER force field and the Ewald summation method, which eliminates the undesired effects of truncation and p...

journal_title：Biophysical journal

authors： Castrignanò T,Chillemi G,Desideri A

更新日期：2000-09-01 00:00:00

abstract：:Cellular differentiation proceeds along complicated pathways, even when it is induced by extracellular signaling molecules. One of the major reasons for this complexity is the highly multidimensional internal dynamics of cells, which sometimes causes apparently stochastic responses in individual cells to extracellular...

journal_title：Biophysical journal

authors： Morita S,Takanezawa S,Hiroshima M,Mitsui T,Ozaki Y,Sako Y

更新日期：2014-11-18 00:00:00

abstract：:The release of GDP from GTPases signals the initiation of a GTPase cycle, where the association of GTP triggers conformational changes promoting binding of downstream effector molecules. Studies have implicated the nucleotide-binding G5 loop to be involved in the GDP release mechanism. For example, biophysical studies...

journal_title：Biophysical journal

authors： Guilfoyle AP,Deshpande CN,Font Sadurni J,Ash MR,Tourle S,Schenk G,Maher MJ,Jormakka M

更新日期：2014-12-16 00:00:00

abstract：:Microsecond rotational motions of nitroxide spin labels are measured primarily with saturation transfer electron paramagnetic resonance (ST-EPR). In the present study we have used model system experiments to quantitatively evaluate different ST-EPR spectral parameters, both in-phase and out-of-phase, with an emphasis ...

journal_title：Biophysical journal

authors： Squier TC,Thomas DD

更新日期：1986-04-01 00:00:00

abstract：:The effect of intracellular (i) and extracellular (o) Na+ on pre-steady-state transient current associated with Na+/Na+ exchange by the Na+/K+ pump was investigated in the vegetal pole of Xenopus oocytes. Current records in response to 40-ms voltage pulses from -180 to +100 mV in the absence of external Na+ were subtr...

journal_title：Biophysical journal

authors： Holmgren M,Rakowski RF

更新日期：2006-03-01 00:00:00

abstract：:The glucocorticoid receptor (GR) is a steroid-hormone-activated transcription factor that modulates gene expression. Transcriptional regulation by the GR requires dynamic receptor binding to specific target sites located across the genome. This binding remodels the chromatin structure to allow interaction with other t...

journal_title：Biophysical journal

authors： Muratcioglu S,Presman DM,Pooley JR,Grøntved L,Hager GL,Nussinov R,Keskin O,Gursoy A

更新日期：2015-09-15 00:00:00

abstract：:Transcription factor (TF) proteins rapidly locate unique target sites on long genomic DNA molecules--and bind to them--during gene regulation. The search mechanism is known to involve a combination of three-dimensional diffusion through the bulk of the cell and one-dimensional sliding diffusion along the DNA. It is be...

journal_title：Biophysical journal

authors： Hu L,Grosberg AY,Bruinsma R

更新日期：2008-08-01 00:00:00

abstract：:The effects of temperature (3 degrees-26 degrees C) on the nonlinear components of the displacement current were measured in internally perfused, voltage clamped squid axons. Steps of potential were applied from a holding potential of -70mV (outside ground) to values from -130 to +70mV and either the current or its in...

journal_title：Biophysical journal

authors： Bezanilla F,Taylor RE

更新日期：1978-09-01 00:00:00

abstract：:In previous in vivo studies, amyloid fibers formed from a peptide ubiquitous in human seminal fluid (semen-derived enhancer of viral infection (SEVI)) were found to dramatically enhance the infectivity of the HIV virus (3-5 orders of magnitude by some measures). To complement those studies, we performed in vitro assay...

journal_title：Biophysical journal

authors： Brender JR,Hartman K,Gottler LM,Cavitt ME,Youngstrom DW,Ramamoorthy A

更新日期：2009-11-04 00:00:00

abstract：:Glucagon solutions at pH 2.0 were subjected to mechanical agitation at 37 degrees C in the presence of a hydrophobic surface to explore the details of aggregation and fiber formation. High-resolution intermittent-contact atomic force microscopy performed in solution revealed the presence of aggregates after 0.5 h; how...

journal_title：Biophysical journal

authors： De Jong KL,Incledon B,Yip CM,DeFelippis MR

更新日期：2006-09-01 00:00:00

abstract：:Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although the crystal structures of dark- and light-adapted states have been determined, the molecular mechanisms underlying photoactivation remain elusive. Here, we demonstrate that the conserved tongue region of...

journal_title：Biophysical journal

authors： Gustavsson E,Isaksson L,Persson C,Mayzel M,Brath U,Vrhovac L,Ihalainen JA,Karlsson BG,Orekhov V,Westenhoff S

更新日期：2020-01-21 00:00:00

abstract：:Osmotic stress in the 0.5-5 x 10(6) dyne/cm2 range was used to perturb the hydration of actin-myosin-ATP intermediates during steady-state hydrolysis. Polyethylene glycol (PEG) (1000 to 4000 Da), in the 1 to 10 wt% range, which does not cause protein precipitation, did not significantly affect the apparent KM or the V...

journal_title：Biophysical journal

authors： Highsmith S,Duignan K,Cooke R,Cohen J

更新日期：1996-06-01 00:00:00

abstract：:The E462R mutation in the fifth position of the AID (alpha1 subunit interaction domain) region in the I-II linker is known to significantly accelerate voltage-dependent inactivation (VDI) kinetics of the L-type CaV1.2 channel, suggesting that the AID region could participate in a hinged-lid type inactivation mechanism...

journal_title：Biophysical journal

authors： Dafi O,Berrou L,Dodier Y,Raybaud A,Sauvé R,Parent L

更新日期：2004-11-01 00:00:00

abstract：:The bovine eye lens protein, alpha L crystallin, has been studied with photon correlation spectroscopy and statical light scattering in the concentration range up to 200 g/l in different solvent conditions. At higher concentration (c greater than 70 g/l) the scattering behavior is quite complicated, which results in n...

journal_title：Biophysical journal

authors： Andries C,Clauwaert J

更新日期：1985-05-01 00:00:00

abstract：:Late-onset heart failure (HF) is a known side effect of doxorubicin chemotherapy. Typically, patients are diagnosed when already at an irreversible stage of HF, which allows few or no treatment options. Identifying the causes of compromised cardiac function in this patient group may improve early patient diagnosis and...

journal_title：Biophysical journal

authors： Lewalle A,Land S,Merken JJ,Raafs A,Sepúlveda P,Heymans S,Kleinjans J,Niederer SA

更新日期：2019-12-17 00:00:00

abstract：:Neal and Cunningham (Neal, M. J., and J. R. Cunningham. 1995. J. Physiol. (Lond.). 482:363-372) showed that GABA(B) agonists and glycinergic antagonists enhance the light-evoked release of retinal acetylcholine. They proposed that glycinergic cells inhibit the cholinergic Starburst amacrine cells and are in turn inhib...

journal_title：Biophysical journal

authors： Grzywacz NM,Zucker CL

更新日期：2006-07-15 00:00:00

abstract：:Cell spreading is involved in many physiological and pathological processes. The spreading behavior of a cell significantly depends on its microenvironment, but the biochemomechanical mechanisms of geometry-confined cell spreading remain unclear. A dynamic model is here established to investigate the spreading of cell...

journal_title：Biophysical journal

authors： Zhao ZL,Liu ZY,Du J,Xu GK,Feng XQ

更新日期：2017-06-06 00:00:00

abstract：:The yolk granule is the most abundant membrane-bound organelle present in sea urchin eggs and embryos. The major protein component of this organelle, toposome, accounts for approximately 50% of the total yolk protein and has been shown to be localized to the embryonic cell surface. Extensive characterization in severa...

journal_title：Biophysical journal

authors： Hayley M,Emberley J,Davis PJ,Morrow MR,Robinson JJ

更新日期：2006-12-15 00:00:00

abstract：:We have interrogated the isothermal folding behavior of the DNA i-motif of the human telomere, dC19, and a high-stability i-motif-forming sequence in the promoter of the human DNA repair gene RAD17 using human physiological solution and temperature conditions. We developed a circular-dichroism-spectroscopy-based pH ti...

journal_title：Biophysical journal

authors： Rogers RA,Fleming AM,Burrows CJ

更新日期：2018-04-24 00:00:00

abstract：:The dynamics of proton transfer between the surface of purple membrane and the aqueous bulk have recently been investigated by the Laser Induced Proton Pulse Method. Following a Delta-function release of protons to the bulk, the system was seen to regain its state of equilibrium within a few hundreds of microseconds. ...

journal_title：Biophysical journal

authors： Schätzler B,Dencher NA,Tittor J,Oesterhelt D,Yaniv-Checover S,Nachliel E,Gutman M

更新日期：2003-01-01 00:00:00

abstract：:The group-additive decomposition of the unfolding free energy of a protein in an osmolyte solution relative to that in water poses a fundamental paradox: whereas the decomposition describes the experimental results rather well, theory suggests that a group-additive decomposition of free energies is, in general, not va...

journal_title：Biophysical journal

authors： Tomar DS,Asthagiri D,Weber V

更新日期：2013-09-17 00:00:00