当前位置： SCI文献检索 > BIOPHYSICAL JOURNAL期刊下所有文献 > Cholesterol-Enriched Domain Formation Induced by Viral-Encoded, Membrane-Active Amphipathic Peptide.

Cholesterol-Enriched Domain Formation Induced by Viral-Encoded, Membrane-Active Amphipathic Peptide.

Abstract：

:The α-helical (AH) domain of the hepatitis C virus nonstructural protein NS5A, anchored at the cytoplasmic leaflet of the endoplasmic reticulum, plays a role in viral replication. However, the peptides derived from this domain also exhibit remarkably broad-spectrum virocidal activity, raising questions about their modes of membrane association. Here, using giant lipid vesicles, we show that the AH peptide discriminates between membrane compositions. In cholesterol-containing membranes, peptide binding induces microdomain formation. By contrast, cholesterol-depleted membranes undergo global softening at elevated peptide concentrations. Furthermore, in mixed populations, the presence of ∼100 nm vesicles of viral dimensions suppresses these peptide-induced perturbations in giant unilamellar vesicles, suggesting size-dependent membrane association. These synergistic composition- and size-dependent interactions explain, in part, how the AH domain might on the one hand segregate molecules needed for viral assembly and on the other hand furnish peptides that exhibit broad-spectrum virocidal activity.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Hanson JM,Gettel DL,Tabaei SR,Jackman J,Kim MC,Sasaki DY,Groves JT,Liedberg B,Cho NJ,Parikh AN

doi

10.1016/j.bpj.2015.11.032

subject

Has Abstract

pub_date

2016-01-05 00:00:00

pages

176-87

issue

1

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(15)01215-1

journal_volume

110

pub_type

杂志文章

相关文献

BIOPHYSICAL JOURNAL文献大全
  • Structures of actomyosin crossbridges in relaxed and rigor muscle fibers.

    abstract：:It was shown previously that a significant fraction of the myosin crossbridges is attached to actin in the skinned rabbit psoas fibers under relaxed conditions at low ionic strength and low temperature (Brenner, B., M. Schoenberg, J. M. Chalovich, L. E. Greene, and E. Eisenberg. 1982. Proc. Natl. Acad. Sci. USA. 79:72...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(89)82838-3

    authors： Yu LC,Brenner B

    更新日期：1989-03-01 00:00:00

  • (In)validity of the constant field and constant currents assumptions in theories of ion transport.

    abstract：:Constant electric fields and constant ion currents are often considered in theories of ion transport. Therefore, it is important to understand the validity of these helpful concepts. The constant field assumption requires that the charge density of permeant ions and flexible polar groups is virtually voltage independe...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(99)77242-5

    authors： Syganow A,von Kitzing E

    更新日期：1999-02-01 00:00:00

  • Bipedal locomotion in crawling cells.

    abstract：:Many complex cellular processes from mitosis to cell motility depend on the ability of the cytoskeleton to generate force. Force-generating systems that act on elastic cytoskeletal elements are prone to oscillating instabilities. In this work, we have measured spontaneous shape and movement oscillations in motile fish...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2009.10.058

    authors： Barnhart EL,Allen GM,Jülicher F,Theriot JA

    更新日期：2010-03-17 00:00:00

  • On mutations that uncouple sodium channel activation from inactivation.

    abstract：:Computations on sodium channel gating were conducted using a closed-open-inactivated coupled kinetic scheme. The time constant of inactivation (tauh) derives a voltage dependency from coupling to voltage-dependent activation even when rate constants between inactivated and other states are strictly voltage independent...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(99)77408-4

    authors： Goldman L

    更新日期：1999-05-01 00:00:00

  • Modulation of titin-based stiffness by disulfide bonding in the cardiac titin N2-B unique sequence.

    abstract：:The giant protein titin is responsible for the elasticity of nonactivated muscle sarcomeres. Titin-based passive stiffness in myocardium is modulated by titin-isoform switching and protein-kinase (PK)A- or PKG-dependent titin phosphorylation. Additional modulatory effects on titin stiffness may arise from disulfide bo...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2009.05.037

    authors： Grützner A,Garcia-Manyes S,Kötter S,Badilla CL,Fernandez JM,Linke WA

    更新日期：2009-08-05 00:00:00

  • The effect of cross-bridge clustering and head-head competition on the mechanical response of skeletal muscle under equilibrium conditions.

    abstract：:The effect of cross-bridge clustering and head-head competition on the mechanical response of skeletal muscle under equilibrium conditions is considered. For this purpose, the recent multiple site equilibrium cross-bridge model of Schoenberg (Schoenberg, M., 1985, Biophys. J., 48:467-475) is extended in accordance wit...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(86)83528-7

    authors： Tözeren A,Schoenberg M

    更新日期：1986-11-01 00:00:00

  • Large-amplitude picosecond anisotropy decay of the intrinsic fluorescence of double-stranded DNA.

    abstract：:The conformational flexibility of the DNA double helix is of great interest because of its potential role in protein recognition, packaging into chromosomes, formation of photodefects, and interaction with drugs. Theory finds that DNA is very flexible; however, there is a scarcity of experimental results that examine ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(96)79755-2

    authors： Georghiou S,Bradrick TD,Philippetis A,Beechem JM

    更新日期：1996-04-01 00:00:00

  • Kinetics of rouleau formation. II. Reversible reactions.

    abstract：:Red blood cells aggregate face-to-face to form long, cylindrical, straight chains and sometimes branched structures called rouleaux. Here we extend a kinetic model developed by R. W. Samsel and A. S. Perelson (1982, Biophys. J. 37:493-514) to include both the formation and dissociation of rouleaux. We examine thermody...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(84)84225-3

    authors： Samsel RW,Perelson AS

    更新日期：1984-04-01 00:00:00

  • On the minimization of fluctuations in the response times of autoregulatory gene networks.

    abstract：:The temporal dynamics of the concentrations of several proteins are tightly regulated, particularly for critical nodes in biological networks such as transcription factors. An important mechanism to control transcription factor levels is through autoregulatory feedback loops where the protein can bind its own promoter...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2011.08.005

    authors： Murugan R,Kreiman G

    更新日期：2011-09-21 00:00:00

  • The Affinity of Sterols for Different Phospholipid Classes and Its Impact on Lateral Segregation.

    abstract：:Cholesterol is an essential molecule in the membranes of mammalian cells. It is known to be distributed heterogeneously within the cells, between the bilayer leaflets, as well as between lateral domains within the bilayer. However, we do not know exactly how cholesterol is distributed and what forces drive this sortin...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2018.11.3135

    authors： Nyholm TKM,Jaikishan S,Engberg O,Hautala V,Slotte JP

    更新日期：2019-01-22 00:00:00

  • Observation volumes and {gamma}-factors in two-photon fluorescence fluctuation spectroscopy.

    abstract：:Fluorescence fluctuation spectroscopy has become an important measurement tool for investigating molecular dynamics, molecular interactions, and chemical kinetics in biological systems. Although the basic theory of fluctuation spectroscopy is well established, it is not widely recognized that saturation of the fluores...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.104.052779

    authors： Nagy A,Wu J,Berland KM

    更新日期：2005-09-01 00:00:00

  • Optical band splitting and electronic perturbations of the heme chromophore in cytochrome C at room temperature probed by visible electronic circular dichroism spectroscopy.

    abstract：:We have measured the electronic circular dichroism (ECD) of the ferri- and ferro-states of several natural cytochrome c derivatives (horse heart, chicken, bovine, and yeast) and the Y67F mutant of yeast in the region between 300 and 750 nm. Thus, we recorded the ECD of the B- and Q-band region as well as the charge-tr...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.106.095976

    authors： Dragomir I,Hagarman A,Wallace C,Schweitzer-Stenner R

    更新日期：2007-02-01 00:00:00

  • Molecular dynamics of alamethicin transmembrane channels from open-channel current noise analysis.

    abstract：:Conductance noise measurement of the open states of alamethicin transmembrane channels reveals excess noise attributable to cooperative low-frequency molecular dynamics that can generate fluctuations approximately 1 A rms in the effective channel pore radius. Single-channel currents through both persistent and nonpers...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(95)80103-7

    authors： Mak DO,Webb WW

    更新日期：1995-12-01 00:00:00

  • Computer Simulation of Radial Immunodiffusion: II. Selection of an Algorithm for the Antibody-Antigen Reaction in Gels.

    abstract：:An algorithm needed for computer simulation of immunodiffusion has been deduced from existing theories of the in vitro reaction between antibody and antigen. The "Goldberg most probable polymer distribution" theory provides a formula that gives the amount of free antibody, free antigen, and diffusible complexes from e...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(73)85996-X

    authors： Trautman R

    更新日期：1973-05-01 00:00:00

  • Proton transfer dynamics on the surface of the late M state of bacteriorhodopsin.

    abstract：:The cytoplasmic surface of the BR (initial) state of bacteriorhodopsin is characterized by a cluster of three carboxylates that function as a proton-collecting antenna. Systematic replacement of most of the surface carboxylates indicated that the cluster is made of D104, E161, and E234 (Checover, S., Y. Marantz, E. Na...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(02)75179-5

    authors： Nachliel E,Gutman M,Tittor J,Oesterhelt D

    更新日期：2002-07-01 00:00:00

  • High field/high frequency saturation transfer electron paramagnetic resonance spectroscopy: increased sensitivity to very slow rotational motions.

    abstract：:Saturation transfer electron paramagnetic resonance (ST-EPR) spectroscopy has been employed to characterize the very slow microsecond to millisecond rotational dynamics of a wide range of nitroxide spin-labeled proteins and other macromolecules in the past three decades. The vast majority of this previous work has bee...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.103.035048

    authors： Hustedt EJ,Beth AH

    更新日期：2004-06-01 00:00:00

  • The connection between chromatin motion on the 100 nm length scale and core histone dynamics in live XTC-2 cells and isolated nuclei.

    abstract：:The diffusive motion of DNA-containing chromatin in live cells and isolated nuclei is investigated using a two-photon standing wave fluorescence photobleaching experiment with 100 nm spatial resolution. The chromatin is labeled using the minor groove binding dye Hoechst 33342. In live cells, the mean diffusion rate is...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(04)74134-X

    authors： Davis SK,Bardeen CJ

    更新日期：2004-01-01 00:00:00

  • In vivo study of chloroplast volume regulation.

    abstract：:This paper describes a new technique that can be used to study chloroplast volume regulation in vivo. Nuclear magnetic resonance spectroscopy was used to measure relative amounts of chloroplast water in Acer platanoides leaves as they dried in air, and also in leaf disks exposed to aqueous polyethylene glycol, sucrose...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(92)81930-6

    authors： McCain DC,Markley JL

    更新日期：1992-05-01 00:00:00

  • Stochastic signal processing and transduction in chemotactic response of eukaryotic cells.

    abstract：:Single-molecule imaging analysis of chemotactic response in eukaryotic cells has revealed a stochastic nature in the input signals and the signal transduction processes. This leads to a fundamental question about the signaling processes: how does the signaling system operate under stochastic fluctuations or noise? Her...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.106.100263

    authors： Ueda M,Shibata T

    更新日期：2007-07-01 00:00:00

  • An information-based approach to change-point analysis with applications to biophysics and cell biology.

    abstract：:This article describes the application of a change-point algorithm to the analysis of stochastic signals in biological systems whose underlying state dynamics consist of transitions between discrete states. Applications of this analysis include molecular-motor stepping, fluorophore bleaching, electrophysiology, partic...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2015.05.038

    authors： Wiggins PA

    更新日期：2015-07-21 00:00:00

  • Equilibrium thermodynamics of cell-cell adhesion mediated by multiple ligand-receptor pairs.

    abstract：:In many situations, cell-cell adhesion is mediated by multiple ligand-receptor pairs. For example, the interaction between T cells and antigen-presenting cells of the immune system is mediated not only by T cell receptors and their ligands (peptide-major histocompatibility complex) but also by binding of intracellular...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(04)74211-3

    authors： Coombs D,Dembo M,Wofsy C,Goldstein B

    更新日期：2004-03-01 00:00:00

  • Proton transport pathway in the ClC Cl-/H+ antiporter.

    abstract：:A fundamental question concerning the ClC Cl-/H+ antiporters is the nature of their proton transport (PT) pathway. We addressed this issue by using a novel computational methodology capable of describing the explicit PT dynamics in the ClC-ec1 protein. The main result is that the Glu203 residue delivers a proton from ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2009.04.038

    authors： Wang D,Voth GA

    更新日期：2009-07-08 00:00:00

  • Lipid Binding Controls Dimerization of the Coat Protein p24 Transmembrane Helix.

    abstract：:Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligo...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2019.09.021

    authors： Pannwitt S,Stangl M,Schneider D

    更新日期：2019-11-05 00:00:00

  • Concentrating engines and the kidney. III. Canonical mass balance equation for multinephron models of the renal medulla.

    abstract：:The canonical mass balance relation derived for the central core model of the renal medulla is extended to medullary models in which an arbitrary assemblage of renal tubules and vascular capillaries exchange with each other both directly and via the medullary interstitium and in which not all of the vascular loops or ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(76)85773-6

    authors： Stephenson JL

    更新日期：1976-11-01 00:00:00

  • Spreading of neutrophils: from activation to migration.

    abstract：:Neutrophils rely on rapid changes in morphology to ward off invaders. Time-resolved dynamics of spreading human neutrophils after activation by the chemoattractant fMLF (formyl methionyl leucyl phenylalanine) was observed by RICM (reflection interference contrast microscopy). An image-processing algorithm was develope...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.105.080382

    authors： Sengupta K,Aranda-Espinoza H,Smith L,Janmey P,Hammer D

    更新日期：2006-12-15 00:00:00

  • DNA release from lipoplexes by anionic lipids: correlation with lipid mesomorphism, interfacial curvature, and membrane fusion.

    abstract：:DNA release from lipoplexes is an essential step during lipofection and is probably a result of charge neutralization by cellular anionic lipids. As a model system to test this possibility, fluorescence resonance energy transfer between DNA and lipid covalently labeled with Cy3 and BODIPY, respectively, was used to mo...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.104.042895

    authors： Tarahovsky YS,Koynova R,MacDonald RC

    更新日期：2004-08-01 00:00:00

  • General continuum theory for multiion channel. II. Application to acetylcholine channel.

    abstract：:The general theory (Levitt, D. G. 1990. Biophys. J. 59:271-277) is applied to a model channel that resembles the acetylcholine receptor channel (ACH). The model incorporates the known features of the ACH geometry and fixed charge locations. The channel has a wide mouth facing the outer solution, tapering to a narrow r...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(91)82221-4

    authors： Levitt DG

    更新日期：1991-02-01 00:00:00

  • Carbohydrate conformation and lipid condensation in monolayers containing glycosphingolipid Gb3: influence of acyl chain structure.

    abstract：:Globotriaosylceramide (Gb3), a glycosphingolipid found in the plasma membrane of animal cells, is the endocytic receptor of the bacterial Shiga toxin. Using x-ray reflectivity (XR) and grazing incidence x-ray diffraction (GIXD), lipid monolayers containing Gb3 were investigated at the air-water interface. XR probed Gb...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2014.07.023

    authors： Watkins EB,Gao H,Dennison AJC,Chopin N,Struth B,Arnold T,Florent JC,Johannes L

    更新日期：2014-09-02 00:00:00

  • Measurement of local viscoelasticity and forces in living cells by magnetic tweezers.

    abstract：:We measured the viscoelastic properties of the cytoplasm of J774 macrophages with a recently developed microrheometer. Ferromagnetic beads (1.3 microm in diameter) were used to determine the local viscoelastic moduli. Step-force pulses were applied to the magnetic beads and the displacement was observed by single part...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(99)77225-5

    authors： Bausch AR,Möller W,Sackmann E

    更新日期：1999-01-01 00:00:00

  • Single-molecule force spectroscopy of cartilage aggrecan self-adhesion.

    abstract：:We investigated self-adhesion between highly negatively charged aggrecan macromolecules extracted from bovine cartilage extracellular matrix by performing atomic force microscopy (AFM) imaging and single-molecule force spectroscopy (SMFS) in saline solutions. By controlling the density of aggrecan molecules on both th...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.09.002

    authors： Harder A,Walhorn V,Dierks T,Fernàndez-Busquets X,Anselmetti D

    更新日期：2010-11-17 00:00:00