Computational Model of Chimeric Antigen Receptors Explains Site-Specific Phosphorylation Kinetics.

abstract：:Myoglobin (Mb) is a model system for ligand binding and migration. The energy barriers (ΔG) for ligand migration in Mb have been studied in the past by experiment and theory and significant differences between different approaches were found. From experiment, it is known that Mb can assume a large number of conformati...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2012-01-18 00:00:00

abstract：:The electric quadrupole moment of the deuterium nucleus provides a nuclear magnetic resonance (NMR) probe of electric field gradients, and thereby of organization of tissue water. 8-17% of H(2)O in rat muscle and brain was replaced by D(2)O from 50% deuterated drinking water. The peak height of the steady-state NMR sp...

journal_title：Biophysical journal

authors： Cope FW

更新日期：1969-03-01 00:00:00

abstract：:The effect of electric fields on dry oriented multibilayers of dimyristoylphosphatidylcholine (DMPC) was investigated by transmission Fourier transform infrared electric field modulated excitation (E-ME) spectroscopy. A periodic rectangular electric potential (0-150 V, 1.25 Hz, 28.4 degrees C +/- 0.2 degrees C) was ap...

journal_title：Biophysical journal

authors： Schwarzott M,Lasch P,Baurecht D,Naumann D,Fringeli UP

更新日期：2004-01-01 00:00:00

abstract：:In previous in vivo studies, amyloid fibers formed from a peptide ubiquitous in human seminal fluid (semen-derived enhancer of viral infection (SEVI)) were found to dramatically enhance the infectivity of the HIV virus (3-5 orders of magnitude by some measures). To complement those studies, we performed in vitro assay...

journal_title：Biophysical journal

authors： Brender JR,Hartman K,Gottler LM,Cavitt ME,Youngstrom DW,Ramamoorthy A

更新日期：2009-11-04 00:00:00

abstract：:Apparent diffusion coefficient (ADC) measurement in the lung using gas magnetic resonance imaging is a promising technique with potential for reflecting changes in lung microstructure. Despite some recent impressive human applications, full interpretation of ADC measures remains an elusive goal, due to a lack of detai...

journal_title：Biophysical journal

authors： Pérez-Sánchez JM,Rodríguez I,Ruiz-Cabello J

更新日期：2009-07-22 00:00:00

abstract：:During replication, the physical state of a virus is controlled by assembly and disassembly processes, when particles are put together and dismantled by cellular cues, respectively. A fundamental question has been how a cell can assemble an infectious virus, and dismantle a virus entering an uninfected cell and thereb...

journal_title：Biophysical journal

authors： Greber UF

更新日期：2014-06-03 00:00:00

abstract：:The rate of exchange of urea across the membranes of human erythrocytes (red blood cells) was quantified on the 1-s to 2-min timescale. (13)C-urea was hyperpolarized and subjected to rapid dissolution and the previously reported (partial) resolution of (13)C NMR resonances from the molecules inside and outside red blo...

journal_title：Biophysical journal

authors： Pagès G,Puckeridge M,Liangfeng G,Tan YL,Jacob C,Garland M,Kuchel PW

更新日期：2013-11-05 00:00:00

abstract：:A model alpha-helical peptide encapsulated in a reverse micelle is used to study the structure and dynamics of proteins under constrained environments that mimic the membrane-water environment in cells. Molecular dynamics simulations of the self assembly of systems composed of a peptide, sodium bis(2-ethylhexyl) sulfo...

journal_title：Biophysical journal

authors： Tian J,Garcia AE

更新日期：2009-05-20 00:00:00

abstract：:The oligomeric state and function of band 3, purified by sulfhydryl affinity chromatography in reduced Triton X-100, was investigated. Size exclusion high-performance liquid chromatography showed that a homogeneous population of band 3 dimers could be purified from whole erythrocyte membranes. The elution profile of b...

journal_title：Biophysical journal

authors： Taylor AM,Boulter J,Harding SE,Cölfen H,Watts A

更新日期：1999-04-01 00:00:00

abstract：:Clathrin-coated membranes are precursors to coated vesicles in the receptor-mediated endocytic pathway. In this paper we present a physical model for the first steps of the transformation of a clathrin-coated membrane into a coated vesicle. The theory is based on in vitro cytoplasmic acidification experiments of Heuse...

journal_title：Biophysical journal

authors： Mashl RJ,Bruinsma RF

更新日期：1998-06-01 00:00:00

abstract：:The vibrational Raman spectra of both pure 1-alpha-dimyristoylphosphatidic acid (DMPA) liposomes and DMPA multilayers reconstituted with ferricytochrome c at pH 7 and pH 4, with either sodium or calcium as the cation, are reported as a function of temperature. Multilayers composed of a 1:1 mol ratio DMPA and dimyristo...

journal_title：Biophysical journal

authors： Vincent JS,Levin IW

更新日期：1991-05-01 00:00:00

abstract：:The mechanism by which the cytoskeletal protein actin affects the conductance of amiloride-sensitive epithelial sodium channels (ENaC) was studied in planar lipid bilayers. In the presence of monomeric actin, we found a decrease in the single-channel conductance of alpha-ENaC that did not occur when the internal [Ca2+...

journal_title：Biophysical journal

authors： Berdiev BK,Latorre R,Benos DJ,Ismailov II

更新日期：2001-05-01 00:00:00

abstract：:Endothelin-1 (ET-1) mediates physiological responses via endothelin A (ET(A)) and B (ET(B)) receptors, which may form homo- and heterodimers with unknown function. Here, we investigated ET-receptor dimerization using fluorescence resonance energy transfer (FRET) between receptors tagged with CFP (donor) and receptors ...

journal_title：Biophysical journal

authors： Evans NJ,Walker JW

更新日期：2008-07-01 00:00:00

abstract：:We introduce an approach based on the recently introduced functional mode analysis to identify collective modes of internal dynamics that maximally correlate to an external order parameter of functional interest. Input structural data can be either experimentally determined structure ensembles or simulated ensembles, ...

journal_title：Biophysical journal

authors： Krivobokova T,Briones R,Hub JS,Munk A,de Groot BL

更新日期：2012-08-22 00:00:00

abstract：:In this article we present a study of a new class of surfactants denoted as PEG1500-12-acyloxystearates, which have potential use as pharmaceutical solubilizers. These amphiphilic molecules present interesting properties with regard to cell damage effects. PEG1500-12-acyloxystearates with C(14) to C(16) acyloxy chains...

journal_title：Biophysical journal

authors： Vaccaro M,von Corswant C,Söderman O

更新日期：2007-12-15 00:00:00

abstract：:Guanidinium and acetamidinium, when added to the bathing solution in concentrations of approximately 0.1M, cause brief blocks in the single channel potassium currents from channels formed in planar lipid bilayers by gramicidin A. Single channel lifetimes are not affected indicating that the channel structure is not mo...

journal_title：Biophysical journal

authors： Hemsley G,Busath D

更新日期：1991-04-01 00:00:00

abstract：:The canonical mass balance relation derived for the central core model of the renal medulla is extended to medullary models in which an arbitrary assemblage of renal tubules and vascular capillaries exchange with each other both directly and via the medullary interstitium and in which not all of the vascular loops or ...

journal_title：Biophysical journal

authors： Stephenson JL

更新日期：1976-11-01 00:00:00

abstract：:Video-based particle tracking monitors the microscopic movement of labeled biomolecules and fluorescent probes within a complex cellular environment. Information gained from this technique enables us to extract the dynamic behavior of biomolecules and the local mechanical properties inside the cell from a tracked part...

journal_title：Biophysical journal

authors： Wu PH,Arce SH,Burney PR,Tseng Y

更新日期：2009-06-17 00:00:00

abstract：:Single-molecule studies allow the study of subtle activity differences due to local folding in proteins, but are time consuming and difficult because only a few molecules are observed in one experiment. We developed an assay where we can simultaneously measure the activity of hundreds of individual molecules. The assa...

journal_title：Biophysical journal

authors： Lee AI,Brody JP

更新日期：2005-06-01 00:00:00

abstract：:Profiles of negatively stained gap junctions have been measured by grid sectioning. After normal levels of electron irradiation, the membrane thickness shrinks to about half that of unirradiated controls, but no shrinkage occurs in the hexagonal lattice plane. Even under low irradiation conditions, there is significan...

journal_title：Biophysical journal

authors： Sosinsky GE,Jésior JC,Caspar DL,Goodenough DA

更新日期：1988-05-01 00:00:00

abstract：:We have measured, using infrared spectroscopy, the hydrogen/deuterium exchange rates of the amide protons in the photosynthetic antenna of Rhodospirillum rubrum. These measurements were made not only on the intact protein in detergent solution but also on two dissociated forms (B820 and B777). We have, on the basis of...

journal_title：Biophysical journal

authors： Sturgis J,Robert B,Goormaghtigh E

更新日期：1998-02-01 00:00:00

abstract：:The electronic structure of the circular aggregate of 18 bacteriochlorophyll a (BChl a) molecules responsible for the B850 absorption band of the light-harvesting 2 (LH2) complex of the photosynthetic purple bacterium Rhodopseudomonas acidophila has been studied by measuring fluorescence-excitation spectra of individu...

journal_title：Biophysical journal

authors： Ketelaars M,van Oijen AM,Matsushita M,Köhler J,Schmidt J,Aartsma TJ

更新日期：2001-03-01 00:00:00

abstract：:A new kinetic model of the Na(+)/H(+) exchanger (NHE) was developed by fitting a variety of major experimental findings, such as ion-dependencies, forward/reverse mode, and the turnover rate. The role of NHE in ion homeostasis was examined by implementing the NHE model in a minimum cell model including intracellular p...

journal_title：Biophysical journal

authors： Cha CY,Oka C,Earm YE,Wakabayashi S,Noma A

更新日期：2009-11-18 00:00:00

abstract：:The cornea is the primary refractive lens in the eye and transmits >90% of incident visible light. It has been suggested that the development of postoperative corneal haze could be due to an increase in light scattering from activated corneal stromal cells. Quiescent keratocytes are thought to produce crystallins that...

journal_title：Biophysical journal

authors： Gardner SJ,White N,Albon J,Knupp C,Kamma-Lorger CS,Meek KM

更新日期：2015-10-20 00:00:00

abstract：:Photoreverse reactions of octopus rhodopsin (Rh) from acid-metarhodopsin (Acid-Meta), which is the final product of the photoreaction of Rh, to Rh were studied by the time-resolved transient absorption and transient grating methods. The time course of the absorption signal showed a rapid change within 500 ns followed ...

journal_title：Biophysical journal

authors： Inoue K,Tsuda M,Terazima M

更新日期：2007-05-15 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) is a sensitive and widely used technique for measuring diffusion. FCS data are conventionally modeled with a finite number of diffusing components and fit with a least-square fitting algorithm. This approach is inadequate for analyzing data obtained from highly heterogeneous...

journal_title：Biophysical journal

authors： Sengupta P,Garai K,Balaji J,Periasamy N,Maiti S

更新日期：2003-03-01 00:00:00

abstract：:The formation and function of the mitotic spindle depends upon force generation by multiple molecular motors and by the dynamics of microtubules, but how these force-generating mechanisms relate to one another is unclear. To address this issue we have modeled the separation of spindle poles as a function of time durin...

journal_title：Biophysical journal

authors： Cytrynbaum EN,Scholey JM,Mogilner A

更新日期：2003-02-01 00:00:00

abstract：:It is accepted that bicarbonate reabsorption in the proximal tubule is mediated by H+ secretion, but several aspects of this process have remained controversial. To examine some of these issues, we have developed a model that allows for spatial variations in the concentrations of CO2, HCO3-, and H2CO3 within the tubul...

journal_title：Biophysical journal

authors： Wang KW,Deen WM

更新日期：1980-08-01 00:00:00

abstract：:The structure of a membrane-embedded alpha-helical reference protein, the M13 major coat protein, is characterized under different conditions of hydrophobic mismatch using fluorescence resonance energy transfer in combination with high-throughput mutagenesis. We show that the structure is similar in both thin (14:1) a...

journal_title：Biophysical journal

authors： Vos WL,Schor M,Nazarov PV,Koehorst RB,Spruijt RB,Hemminga MA

更新日期：2007-11-15 00:00:00

abstract：:A novel combined approach of molecular dynamics (MD) and Monte Carlo simulations is developed to calculate various free-volume distributions as a function of position in a lipid bilayer membrane at 323 K. The model bilayer consists of 2 x 100 chain molecules with each chain molecule having 15 carbon segments and one h...

journal_title：Biophysical journal

authors： Xiang TX

更新日期：1993-09-01 00:00:00