Measuring the Refractive Index of Bovine Corneal Stromal Cells Using Quantitative Phase Imaging.

abstract：:We measured the exocytotic response induced by flash photolysis of caged compounds in isolated mast cells and chromaffin cells. Vesicle fusion was measured by monitoring the cell membrane capacitance. The release of vesicular contents was followed by amperometry. In response to a GTP gamma S stimulus we found that the...

journal_title：Biophysical journal

authors： Oberhauser AF,Robinson IM,Fernandez JM

更新日期：1996-08-01 00:00:00

abstract：:We have examined the distribution of ryanodine receptors, L-type Ca(2+) channels, calsequestrin, Na(+)/Ca(2+) exchangers, and voltage-gated Na(+) channels in adult rat ventricular myocytes. Enzymatically dissociated cells were fixed and dual-labeled with specific antibodies using standard immunocytochemistry protocols...

journal_title：Biophysical journal

authors： Scriven DR,Dan P,Moore ED

更新日期：2000-11-01 00:00:00

abstract：:In this article, we demonstrate the new method of pulsed interleaved excitation (PIE), which can be used to extend the capabilities of multiple-color fluorescence imaging, fluorescence cross-correlation spectroscopy (FCCS), and single-pair fluorescence resonance energy transfer (spFRET) measurements. In PIE, multiple ...

journal_title：Biophysical journal

authors： Müller BK,Zaychikov E,Bräuchle C,Lamb DC

更新日期：2005-11-01 00:00:00

abstract：:The present article considers the influence of heterogeneity in a mobile analyte or in an immobilized ligand population on the surface binding kinetics and equilibrium isotherms. We describe strategies for solving the inverse problem of calculating two-dimensional distributions of rate and affinity constants from expe...

journal_title：Biophysical journal

authors： Svitel J,Balbo A,Mariuzza RA,Gonzales NR,Schuck P

更新日期：2003-06-01 00:00:00

abstract：:Intramolecular electron transfer between CuA and heme a in solubilized bacterial (Paracoccus denitrificans) cytochrome c oxidase was investigated by pulse radiolysis. CuA, the initial electron acceptor, was reduced by 1-methylnicotinamide radicals in a diffusion-controlled reaction, as monitored by absorption changes ...

journal_title：Biophysical journal

authors： Farver O,Grell E,Ludwig B,Michel H,Pecht I

更新日期：2006-03-15 00:00:00

abstract：:To investigate the mechanism of interaction of gramicidin S-like antimicrobial peptides with biological membranes, a series of five decameric cyclic cationic beta-sheet-beta-turn peptides with all possible combinations of aromatic D-amino acids, Cyclo(Val-Lys-Leu-D-Ar1-Pro-Val-Lys-Leu-D-Ar2-Pro) (Ar identical with Phe...

journal_title：Biophysical journal

authors： Jelokhani-Niaraki M,Hodges RS,Meissner JE,Hassenstein UE,Wheaton L

更新日期：2008-10-01 00:00:00

abstract：:A model for the action of vinblastine (VLB) on cells multiplying exponentially in vivo with a generation time, T(G), has been derived. It is based on the assumption that cells attempting to pass through mitosis in the presence of VLB lose their proliferative capacity and that this lethal effect occurs only when the ce...

journal_title：Biophysical journal

authors： Valeriote FA,Bruce WR,Meeker BE

更新日期：1966-03-01 00:00:00

abstract：:The influence of basic open-loop synaptic connections on the firing of simultaneously recorded neurons has been investigated with auto- and cross-correlation histograms, using experimental records and computer simulations. The basic connections examined were direct synaptic excitation, direct synaptic inhibition, and ...

journal_title：Biophysical journal

authors： Moore GP,Segundo JP,Perkel DH,Levitan H

更新日期：1970-09-01 00:00:00

abstract：:Photosystem II contains two well-characterized tyrosine radicals, D(.) and Z(.). Z is an electron carrier between the primary chlorophyll donor and the manganese catalytic site and is essential for enzymatic function. On the other hand, D forms a stable radical with no known role in oxygen evolution. D(.) and Z(.) giv...

journal_title：Biophysical journal

authors： Ma C,Barry BA

更新日期：1996-10-01 00:00:00

abstract：:The vibrational Raman spectra of both pure 1-alpha-dimyristoylphosphatidic acid (DMPA) liposomes and DMPA multilayers reconstituted with ferricytochrome c at pH 7 and pH 4, with either sodium or calcium as the cation, are reported as a function of temperature. Multilayers composed of a 1:1 mol ratio DMPA and dimyristo...

journal_title：Biophysical journal

authors： Vincent JS,Levin IW

更新日期：1991-05-01 00:00:00

abstract：:Kinetic measurements of the self-assembly of proteins into amyloid fibrils are often used to make inferences about molecular mechanisms. In particular, the lag time--the quiescent period before aggregates are detected--is often found to scale with the protein concentration as a power law, whose exponent has been used ...

journal_title：Biophysical journal

authors： Eden K,Morris R,Gillam J,MacPhee CE,Allen RJ

更新日期：2015-02-03 00:00:00

abstract：:Fibrin polymerizes to produce branching fibers forming a three-dimensional network, which has been difficult to visualize by conventional microscopy. Three-dimensional images of whole clots at high resolution were obtained from stereo-pair intermediate-voltage electron micrographs. Computer software was developed to p...

journal_title：Biophysical journal

authors： Baradet TC,Haselgrove JC,Weisel JW

更新日期：1995-04-01 00:00:00

abstract：:It is now rare to find biological, or genetic investigations that do not rely on the tools, data, and thinking drawn from the genomic sciences. Much of this revolution is powered by contemporary sequencing approaches that readily deliver large, genome-wide data sets that not only provide genetic insights but also uniq...

journal_title：Biophysical journal

authors： Schwartz DC

更新日期：2019-12-03 00:00:00

abstract：:The three-dimensional structure of chromatin affects DNA accessibility and is therefore a key regulator of gene expression. However, the path of the DNA between consecutive nucleosomes, and the resulting chromatin fiber organization remain controversial. The conformational space available for the folding of the nucleo...

journal_title：Biophysical journal

authors： Stehr R,Schöpflin R,Ettig R,Kepper N,Rippe K,Wedemann G

更新日期：2010-03-17 00:00:00

abstract：:Connectin (titin) is a large filamentous protein (single peptide) with a molecular mass of approximately 3 MDa, contour length approximately 900 nm, and diameter approximately 4 nm, and resides in striated muscle. Connectin links the thick filaments to the Z-lines in a sarcomere and produces a passive elastic force wh...

journal_title：Biophysical journal

authors： Higuchi H,Nakauchi Y,Maruyama K,Fujime S

更新日期：1993-11-01 00:00:00

abstract：:Branched cable voltage recording and voltage clamp analytical solutions derived in two previous papers are used to explore practical issues concerning voltage clamp. Single exponentials can be fitted reasonably well to the decay phase of clamped synaptic currents, although they contain many underlying components. The ...

journal_title：Biophysical journal

authors： Major G

更新日期：1993-07-01 00:00:00

abstract：:The calcium release channels/ryanodine receptors (RyRs) usually form two-dimensional regular lattices in the endoplasmic/sarcoplasmic reticulum membranes. However, the function and modulation of the interaction between neighboring RyRs are still unknown. Here, with an in vitro aqueous system, we demonstrate that the i...

journal_title：Biophysical journal

authors： Hu XF,Liang X,Chen KY,Xie H,Xu Y,Zhu PH,Hu J

更新日期：2005-09-01 00:00:00

abstract：:We find evidence for a direct transition of insulin monomers into amyloid fibrils without measurable concentrations of oligomers or protofibrils, suggesting that fibrillogenesis may occur directly from assembly of denaturing insulin monomers rather than by successive transitions through protofibril nuclei. To support ...

journal_title：Biophysical journal

authors： Pease LF 3rd,Sorci M,Guha S,Tsai DH,Zachariah MR,Tarlov MJ,Belfort G

更新日期：2010-12-15 00:00:00

abstract：:Rapid conduction of nerve impulses requires coating of axons by myelin sheaths, which are multilamellar, lipid-rich membranes produced by oligodendrocytes in the central nervous system. To act as an insulator, myelin has to form a stable and firm membrane structure. In this study, we have analyzed the biophysical prop...

journal_title：Biophysical journal

authors： Yurlova L,Kahya N,Aggarwal S,Kaiser HJ,Chiantia S,Bakhti M,Pewzner-Jung Y,Ben-David O,Futerman AH,Brügger B,Simons M

更新日期：2011-12-07 00:00:00

abstract：:We present a minimal model of plasma membrane heterogeneity that combines criticality with connectivity to cortical cytoskeleton. The development of this model was motivated by recent observations of micron-sized critical fluctuations in plasma membrane vesicles that are detached from their cortical cytoskeleton. We i...

journal_title：Biophysical journal

authors： Machta BB,Papanikolaou S,Sethna JP,Veatch SL

更新日期：2011-04-06 00:00:00

abstract：:The effect of peptides, corresponding to sequences in the skeletal muscle dihydropyridine receptor II-III loop, on Ca(2+) release from sarcoplasmic reticulum (SR) and on ryanodine receptor (RyR) calcium release channels have been compared in preparations from normal and malignant hyperthermia (MH)-susceptible pigs. Pe...

journal_title：Biophysical journal

authors： Gallant EM,Curtis S,Pace SM,Dulhunty AF

更新日期：2001-04-01 00:00:00

abstract：:Single-molecule methods provide direct measurements of macromolecular dynamics, but are limited by the number of degrees of freedom that can be followed at one time. High-resolution rotor bead tracking (RBT) measures DNA torque, twist, and extension, and can be used to characterize the structural dynamics of DNA and d...

journal_title：Biophysical journal

authors： Ivanov IE,Lebel P,Oberstrass FC,Starr CH,Parente AC,Ierokomos A,Bryant Z

更新日期：2018-01-23 00:00:00

abstract：:Pyranine (8-hydroxy pyrene-1,3,6-trisulfonate) is a commonly used photoacid that discharges a proton when excited to its first electronic singlet state. Follow-up of its dissociation kinetics reveals the physicochemical properties of its most immediate environment. At vanishing ionic strength the dye adsorbs to the Es...

journal_title：Biophysical journal

authors： Nachliel E,Pollak N,Huppert D,Gutman M

更新日期：2001-03-01 00:00:00

abstract：:Chromatin organization within the nucleus is a vital regulator of genome function, yet its mechanical coupling to the nuclear architecture has remained elusive. To directly investigate this coupling, we locally modulated chromatin structure in living cells using nanoparticle-based laser perturbation. Unusual differenc...

journal_title：Biophysical journal

authors： Mazumder A,Shivashankar GV

更新日期：2007-09-15 00:00:00

abstract：:Cardiac excitation-contraction coupling is initialized by the release of Ca from the sarcoplasmic reticulum (SR) in response to a sudden increase in local cytosolic [Ca] ([Ca]i) within the junctional cleft. We have tested the hypothesis that functional ryanodine receptor (RyR) regulation plays a major role in the regu...

journal_title：Biophysical journal

authors： Shannon TR,Wang F,Bers DM

更新日期：2005-12-01 00:00:00

abstract：:Formation of liquid-ordered domains in model membranes can be linked to raft formation in cellular membranes. The lipid stoichiometry has a governing influence on domain formation and consequently, biochemical hydrolysis of specific lipids has the potential to remodel domain features. Activation of phospholipase A(2) ...

journal_title：Biophysical journal

authors： Simonsen AC

更新日期：2008-05-15 00:00:00

abstract：:Isothermal titration calorimetry was used to characterize and quantify the partition of indomethacin and acemetacin between the bulk aqueous phase and the membrane of egg phosphatidylcholine vesicles. Significant electrostatic effects were observed due to binding of the charged drugs to the membrane, which implied the...

journal_title：Biophysical journal

authors： Matos C,Lima JL,Reis S,Lopes A,Bastos M

更新日期：2004-02-01 00:00:00

abstract：:The thermophilic Bacillus caldolyticus cold shock protein (Bc-Csp) differs from the mesophilic Bacillus subtilis cold shock protein B (Bs-CspB) in 11 of the 66 residues. Stability measurements of Schmid and co-workers have implicated contributions of electrostatic interactions to the thermostability. To further elucid...

journal_title：Biophysical journal

authors： Zhou HX,Dong F

更新日期：2003-04-01 00:00:00

abstract：:The outer membrane porin OmpF from Escherichia coli has been reconstituted into lipid bilayers of defined composition, and fluorescence spectroscopy is used to characterize its interaction with the surrounding lipid. OmpF is a trimer within the membrane. It contains two Trp residues per monomer, Trp(214) at the lipid-...

journal_title：Biophysical journal

authors： O'Keeffe AH,East JM,Lee AG

更新日期：2000-10-01 00:00:00

abstract：:The atomic force microscopy (AFM) indentation method combined with the brush model can be used to separate the mechanical response of the cell body from deformation of the pericellular layer surrounding biological cells. Although self-consistency of the brush model to derive the elastic modulus of the cell body has be...

journal_title：Biophysical journal

authors： Dokukin M,Ablaeva Y,Kalaparthi V,Seluanov A,Gorbunova V,Sokolov I

更新日期：2016-07-12 00:00:00