Nonequilibrium behavior in supported lipid membranes containing cholesterol.

abstract：:We present a theoretical analysis of intracellular calcium waves propagated by calcium feedback at the inositol 1,4,5-trisphosphate (IP3) receptor. The model includes essential features of calcium excitability, but is still analytically tractable. Formulas are derived for the wave speed, amplitude, and width. The calc...

journal_title：Biophysical journal

authors： Kupferman R,Mitra PP,Hohenberg PC,Wang SS

更新日期：1997-06-01 00:00:00

abstract：:Osmotic stress in the 0.5-5 x 10(6) dyne/cm2 range was used to perturb the hydration of actin-myosin-ATP intermediates during steady-state hydrolysis. Polyethylene glycol (PEG) (1000 to 4000 Da), in the 1 to 10 wt% range, which does not cause protein precipitation, did not significantly affect the apparent KM or the V...

journal_title：Biophysical journal

authors： Highsmith S,Duignan K,Cooke R,Cohen J

更新日期：1996-06-01 00:00:00

abstract：:The mechanism of high-voltage pulse-induced permeabilization of the stratum corneum, the outer layer of the skin, is still not completely understood. It has been suggested that joule heating resulting from the applied pulse may play a major role in disrupting the stratum corneum. In this study, electrical and ultrastr...

journal_title：Biophysical journal

authors： Gallo SA,Sen A,Hensen ML,Hui SW

更新日期：2002-01-01 00:00:00

abstract：:In recent in vitro experiments, it has been demonstrated that the 47-kDa fragment of the talin molecule and the 32-kDa fragment of the vinculin molecule interact with acidic phospholipids. By using a computer analysis method, we determined the hydrophobic and amphipathic stretches of these fragments and, by applying a...

journal_title：Biophysical journal

authors： Tempel M,Goldmann WH,Isenberg G,Sackmann E

更新日期：1995-07-01 00:00:00

abstract：:The design and implementation of a high-speed, random-access, laser-scanning fluorescence microscope configured to record fast physiological signals from small neuronal structures with high spatiotemporal resolution is presented. The laser-scanning capability of this nonimaging microscope is provided by two orthogonal...

journal_title：Biophysical journal

authors： Bullen A,Patel SS,Saggau P

更新日期：1997-07-01 00:00:00

abstract：:Molecular dynamics (MD) simulations of 5 ns on protein-DNA complexes of catabolite-activator protein (CAP), lambda-repressor, and their corresponding uncomplexed protein and DNA, are reported. These cases represent two extremes of DNA bending, with CAP DNA bent severely and the lambda-operator nearly straight when com...

journal_title：Biophysical journal

authors： Dixit SB,Andrews DQ,Beveridge DL

更新日期：2005-05-01 00:00:00

abstract：:A requirement for concentrated and chemically homogeneous pools of molecular building blocks would severely restrict plausible scenarios for the origin of life. In the case of membrane self-assembly, models of prebiotic lipid synthesis yield primarily short, single-chain amphiphiles that can form bilayer vesicles only...

journal_title：Biophysical journal

authors： Budin I,Prwyes N,Zhang N,Szostak JW

更新日期：2014-10-07 00:00:00

abstract：:X-ray edge absorption of copper and extended fine structure studies of both copper and iron centers have been made of cytochrome oxidase from beef heart, Paracoccus dentrificans, and HB-8 thermophilic bacteria (1-2.5 mM in heme). The desired redox state (fully oxidized, reduced CO, mixed valence formate and CO) in the...

journal_title：Biophysical journal

authors： Powers L,Chance B,Ching Y,Angiolillo P

更新日期：1981-06-01 00:00:00

abstract：:We present a molecular model of a chromatophore vesicle from Rhodobacter sphaeroides. These vesicles are ideal benchmark systems for molecular and systemic simulations, because they have been well studied, they are small, and they are naturally separated from their cellular environment. To set up a photosynthetic chai...

journal_title：Biophysical journal

authors： Geyer T,Helms V

更新日期：2006-08-01 00:00:00

abstract：:In addition to the annular or boundary lipids that surround the transmembrane surface of the potassium channel KcsA from Streptomyces lividans, x-ray crystallographic studies have detected one anionic lipid molecule bound at each protein-protein interface in the homotetrameric structure, at sites referred to as nonann...

journal_title：Biophysical journal

authors： Marius P,Zagnoni M,Sandison ME,East JM,Morgan H,Lee AG

更新日期：2008-03-01 00:00:00

abstract：:Estimates of the free myoplasmic [Ca2+] ([Ca2+]i) with fluorescent dyes are complicated by the fact that some properties of these dyes are altered in the intracellular environment. In the present study indo-1 was used to measure [Ca2+]i in isolated muscle fibers from Xenopus frogs. Fluorescent ratio signals obtained f...

journal_title：Biophysical journal

authors： Westerblad H,Allen DG

更新日期：1996-08-01 00:00:00

abstract：:This study presents what is, to our knowledge, a novel technique by means of which the ratio of the single gating charges of voltage-gated rat brain IIA (rBIIA) sodium and Shaker potassium ion channels was estimated. In the experiment, multiple tandems of enhanced green fluorescent protein were constructed and inserte...

journal_title：Biophysical journal

authors： Gamal El-Din TM,Grögler D,Lehmann C,Heldstab H,Greeff NG

更新日期：2008-08-01 00:00:00

abstract：:Air dried films of purple membranes (PM) from Halobacterium halobium containing the photochromic protein bacteriorhodopsin (BR) were prepared and the BR-photocycle of this material analyzed. The absorption maxima of the initial state B (lambda(max) = 570 nm) and the photochemical intermediate M (lambda(max) = 412 nm),...

journal_title：Biophysical journal

authors： Hampp N,Bräuchle C,Oesterhelt D

更新日期：1990-07-01 00:00:00

abstract：:Biological spread cells exist in a perpetually fluctuating state and therefore cannot be described in terms of a unique deterministic system. For modeling approaches to provide novel insight and uncover new mechanisms that drive cell behavior, a framework is required that progresses from traditional deterministic meth...

journal_title：Biophysical journal

authors： McEvoy E,Shishvan SS,Deshpande VS,McGarry JP

更新日期：2018-12-18 00:00:00

abstract：:The elastic properties of cylindrical segments of sarcolemma were studied in single striated fibers of the frog semitendinosus muscle. All measurements were made on membranes of retraction zones, cell segments from which the sarcoplasm had retracted. Quantitative morphological studies indicated that three deforming fo...

journal_title：Biophysical journal

authors： Fields RW

更新日期：1970-05-01 00:00:00

abstract：:Using a method they developed, Stamatoff and Krimm (1976) have phased swelling data from nerve myelin. Although most phases agree with those I determined previously, there are a few differences. In this letter the two different phasings, theirs and my own, are used to compute the corresponding electron-density profile...

journal_title：Biophysical journal

authors： Blaurock AE

更新日期：1979-04-01 00:00:00

abstract：:Heme proteins react inhomogeneously with ligands at cryogenic temperatures and homogeneously at room temperature. We have identified and characterized a transition from inhomogeneous to homogeneous behavior at intermediate temperatures in the time dependence of CO binding to horse myoglobin. The turnover is attributed...

journal_title：Biophysical journal

authors： Agmon N,Doster W,Post F

更新日期：1994-05-01 00:00:00

abstract：:We have monitored the organization and dynamics of the hemolytic peptide melittin in membranes containing cholesterol by utilizing the intrinsic fluorescence properties of its functionally important sole tryptophan residue and circular dichroism spectroscopy. The significance of this study is based on the fact that th...

journal_title：Biophysical journal

authors： Raghuraman H,Chattopadhyay A

更新日期：2004-10-01 00:00:00

abstract：:Stress fibers are cellular contractile actomyosin machines central to wound healing, shear stress response, and other processes. Contraction mechanisms have been difficult to establish because stress fibers in cultured cells typically exert isometric tension and present little kinetic activity. In a recent study, livi...

journal_title：Biophysical journal

authors： Stachowiak MR,O'Shaughnessy B

更新日期：2009-07-22 00:00:00

abstract：:We investigated the accessibility to protons and thiol-directed reagents of a cysteine substituted at position 338 in transmembrane segment 6 (TM6) of CFTR to test the hypothesis that T338 resides in the pore. Xenopus oocytes expressing T338C CFTR exhibited pH-dependent changes in gCl and I-V shape that were specific ...

journal_title：Biophysical journal

authors： Liu X,Zhang ZR,Fuller MD,Billingsley J,McCarty NA,Dawson DC

更新日期：2004-12-01 00:00:00

abstract：:Intrinsically disordered proteins play important roles throughout biology, yet our understanding of the relationship between their sequences, structural properties, and functions remains incomplete. The dynamic nature of these proteins, however, makes them difficult to characterize structurally. Many disordered protei...

journal_title：Biophysical journal

authors： Nygaard M,Kragelund BB,Papaleo E,Lindorff-Larsen K

更新日期：2017-08-08 00:00:00

abstract：:Mycolactone, a cytotoxic and immunosuppressive macrolide produced by Mycobacterium ulcerans, is the central virulent factor in the skin disease Buruli ulcer. This multifunctional cytotoxin affects fundamental cellular processes such as cell adhesion, immune response, and cell death by targeting various cellular struct...

journal_title：Biophysical journal

authors： Aydin F,Sun R,Swanson JMJ

更新日期：2019-07-09 00:00:00

abstract：:Vinculin activation plays a critical role in focal adhesion initiation and formation. In its native state, vinculin is in an autoinhibitory conformation in which domain 1 prevents interaction of the vinculin tail domain with actin by steric hindrance. Once activated, vinculin is able to interact with both actin and ta...

journal_title：Biophysical journal

authors： Golji J,Mofrad MR

更新日期：2010-08-09 00:00:00

abstract：:Dimerization of the transmembrane (TM) adaptor protein DAP12 plays a key role in mediating activation signals through TM-TM association with cell-surface receptors. Herein, we apply the TOXCAT assay and molecular dynamics simulation to analyze dynamics and dimerization of the TM helix of DAP12 in the membrane bilayer....

journal_title：Biophysical journal

authors： Wei P,Zheng BK,Guo PR,Kawakami T,Luo SZ

更新日期：2013-04-02 00:00:00

abstract：:Unlike most transmembrane proteins, phospholipids can migrate from one leaflet of the membrane to the other. Because this spontaneous lipid translocation (flip-flop) tends to be very slow, cells facilitate the process with enzymes that catalyze the transmembrane movement and thereby regulate the transbilayer lipid dis...

journal_title：Biophysical journal

authors： Doktorova M,Heberle FA,Marquardt D,Rusinova R,Sanford RL,Peyear TA,Katsaras J,Feigenson GW,Weinstein H,Andersen OS

更新日期：2019-03-05 00:00:00

abstract：:Uncovering mechanisms that control the dynamics of microtubules is fundamental for our understanding of multiple cellular processes such as chromosome separation and cell motility. Building on previous theoretical work on the dynamic instability of microtubules, we propose here a stochastic model that includes all rel...

journal_title：Biophysical journal

authors： Padinhateeri R,Kolomeisky AB,Lacoste D

更新日期：2012-03-21 00:00:00

abstract：:Tagging of individual proteins with genetically encoded fluorescent proteins (FPs) has been used extensively to study localization and interactions in live cells. Recent developments in single-molecule localization microscopy have enabled the dynamic visualization of individual tagged proteins inside living cells. How...

journal_title：Biophysical journal

authors： Ghodke H,Caldas VE,Punter CM,van Oijen AM,Robinson A

更新日期：2016-07-12 00:00:00

abstract：:Synthetic filaments prepared from column-purified rabbit skeletal myosin by slow dialysis exhibit characteristic bipolar organization and 14-nm axial subunit spacing. Backbone substructure can be discerned in high resolution micrographs in the form of striations of 3--4-nm width and slight angular tilt from the direct...

journal_title：Biophysical journal

authors： Koretz JF

更新日期：1979-09-01 00:00:00

abstract：:The voltage dependence of light-induced proton pumping was studied with bacteriorhodopsin (bR) from Halobacterium salinarum, expressed in the plasma membrane of oocytes from Xenopus laevis in the range -160 mV to +60 mV at different light intensities. Depending on the applied field, the quenching effect by blue light,...

journal_title：Biophysical journal

authors： Nagel G,Kelety B,Möckel B,Büldt G,Bamberg E

更新日期：1998-01-01 00:00:00

abstract：:A number of models proposed to account for the sodium conductance changes are shown to fall into two classes. The Hodgkin-Huxley (HH) model falls into a class (I) in which the conductance depends on two or more independent variables controlled by independent processes. The Mullins, Hoyt, and Goldman models fall into c...

journal_title：Biophysical journal

authors： Hoyt RC

更新日期：1968-10-01 00:00:00