Accurate determination of membrane dynamics with line-scan FCS.

abstract：:The rotation of human red blood cells (RBC) as they flow in the shear field established by a Coulter type orifice is modeled. This model, based on hydrodynamics of ellipsoid rotation in laminar creeping flow, is used to calculate the probability of the cells entering the orifice with a specific orientation. The electr...

journal_title：Biophysical journal

authors： Breitmeyer MO,Lightfoot EN,Dennis WH

更新日期：1971-02-01 00:00:00

abstract：:The stability of the 1:1 complex of sodium ion with the dianion of guanosine 5'-monophosphate has been determined by means of a potentiometric titration employing a specific ion electrode. The stability constant for the reaction Na(+) + 5'-GMP(2-) Na(5'-GMP)(-) was found to be 2.85 +/- 0.36 M(-1) at 5 degrees C and an...

journal_title：Biophysical journal

authors： Walmsley JA,Pinnavaia TJ

更新日期：1982-06-01 00:00:00

abstract：:The aquaglyceroporin GlpF is a transmembrane channel of Escherichia coli that facilitates the uptake of glycerol by the cell. Its high glycerol uptake rate is crucial for the cell to survive in very low glycerol concentrations. Although GlpF allows both influx and outflux of glycerol, its structure, similar to the str...

journal_title：Biophysical journal

authors： Lu D,Grayson P,Schulten K

更新日期：2003-11-01 00:00:00

abstract：:Cationic lipid-DNA (CL-DNA) complexes are abundantly used in nonviral gene therapy clinical applications. Surface functionality is the next step in developing these complexes as competent, target-specific gene carriers. Poly(ethylene glycol) (PEG) is the natural choice to serve as a protective coat or act as a tether ...

journal_title：Biophysical journal

authors： Martin-Herranz A,Ahmad A,Evans HM,Ewert K,Schulze U,Safinya CR

更新日期：2004-02-01 00:00:00

abstract：:Membranes of the lens of the eye of mammals have two particular characteristics, high concentrations of sphingomyelin, and dihydrosphingomyelin and cholesterol. We have studied the miscibility of cholesterol with both egg sphingomyelin and with dihydrosphingomyelin made by hydrogenation of egg sphingomyelin. At a chol...

journal_title：Biophysical journal

authors： Epand RM

更新日期：2003-05-01 00:00:00

abstract：:Understanding the information processing ability of signal transduction pathways is of great importance because of their crucial roles in triggering various cellular responses. Despite continuing theoretical investigation, some important aspects of signal transduction such as a transient response and its connection to...

journal_title：Biophysical journal

authors： Morishita Y,Kobayashi TJ,Aihara K

更新日期：2006-09-15 00:00:00

abstract：:We have studied the low-resolution solution conformation of a Holliday (or four-way) DNA junction by using small-angle x-ray scattering, sedimentation velocity, and computational modeling techniques. The scattering data were analyzed in two independent ways: firstly, by rigid-body modeling of the scattering data using...

journal_title：Biophysical journal

authors： Nöllmann M,Stark WM,Byron O

更新日期：2004-05-01 00:00:00

abstract：:High speed cinemicrographs were made of sea urchin sperm at temperatures varying from 22 to 6 degrees C. Apparatus, combining a television camera and a video digitizer, was constructed to scan individual flagellar images and to digitize the flagellar waveforms. With appropriate smoothing and averaging procedures, the ...

journal_title：Biophysical journal

authors： Rikmenspoel R,Isles CA

更新日期：1985-03-01 00:00:00

abstract：:Cellular plasma membranes have domains that are defined, in most cases, by cytoskeletal elements. The outer half of the bilayer may also contain domains that organize glycosylphosphatidylinositol (GPI)-linked proteins. To define outer membrane barriers, we measured the resistive force on membrane bound beads as they w...

journal_title：Biophysical journal

authors： Suzuki K,Sterba RE,Sheetz MP

更新日期：2000-07-01 00:00:00

abstract：:Small, icosahedral double-stranded DNA bacteriophage pack their genomes tightly into preformed protein capsids using an ATP-driven motor. Coarse-grain molecular-mechanics models provide a detailed picture of DNA packaging in bacteriophage, revealing how conformation depends on capsid size and shape, and the presence o...

journal_title：Biophysical journal

authors： Petrov AS,Harvey SC

更新日期：2008-07-01 00:00:00

abstract：:The electrophoretic behavior of defined DNA and RNA oligonucleotide duplexes from 10 to 20 bp in length has been investigated as a function of salt conditions, gel concentration, and temperature. The RNA oligomers migrated much more slowly than the DNA oligomers of the same sequence under all conditions. From sediment...

journal_title：Biophysical journal

authors： Bonifacio GF,Brown T,Conn GL,Lane AN

更新日期：1997-09-01 00:00:00

abstract：:Whether proteins or lipids are the primary target sites for general anesthetic action has engendered considerable debate. Recent in vivo studies have shown that the S(+) and R(-) enantiomers of isoflurane are not equipotent, implying involvement of proteins. Bovine serum albumin (BSA), a soluble protein devoid of lipi...

journal_title：Biophysical journal

authors： Xu Y,Tang P,Firestone L,Zhang TT

更新日期：1996-01-01 00:00:00

abstract：:We measure the organization and substructure of HT29 epithelial cells in a monolayer using angle-resolved low-coherence interferometry. This new technique probes cellular structure by measuring scattered light, as in flow cytometry, but offers an advantage in that the structure can be examined in situ, avoiding the ne...

journal_title：Biophysical journal

authors： Wax A,Yang C,Backman V,Badizadegan K,Boone CW,Dasari RR,Feld MS

更新日期：2002-04-01 00:00:00

abstract：:Cell spreading is involved in many physiological and pathological processes. The spreading behavior of a cell significantly depends on its microenvironment, but the biochemomechanical mechanisms of geometry-confined cell spreading remain unclear. A dynamic model is here established to investigate the spreading of cell...

journal_title：Biophysical journal

authors： Zhao ZL,Liu ZY,Du J,Xu GK,Feng XQ

更新日期：2017-06-06 00:00:00

abstract：:The role of distance-dependent anisotropic reactivity and molecular geometry in the vicinity of localized reaction centers in influencing the rate of bimolecular diffusion-controlled reactions is analyzed in detail, both analytically and numerically. The effect of local molecular shape is considered within the model o...

journal_title：Biophysical journal

authors： Shushin AI,Barzykin AV

更新日期：2001-12-01 00:00:00

abstract：:Fluorescence lifetimes of dimeric rabbit muscle creatine kinase specifically dansylated at both active sites and the homologous monomeric lobster muscle arginine kinase singly dansylated were determined using phase-modulation methods with global analysis of overdetermined data sets. For both proteins, the data is adeq...

journal_title：Biophysical journal

authors： Grossman SH

更新日期：1991-03-01 00:00:00

abstract：:Nanopatterned surfaces are believed to kill bacteria through physical deformation, a mechanism that has immense potential against biochemical resistance. Because of its elusive nature, this mechanism is mostly understood through biophysical modeling. Problematically, accurate descriptions of the contact mechanics and ...

journal_title：Biophysical journal

authors： Velic A,Hasan J,Li Z,Yarlagadda PKDV

更新日期：2021-01-19 00:00:00

abstract：:Analyses of receptor-ligand interactions are important to the understanding of cellular adhesion. Traditional methods of measuring the three-dimensional (3D) dissociation constant (Kd) require at least one of the molecular species in solution and hence cannot be directly applied to the case of cell adhesion. We descri...

journal_title：Biophysical journal

authors： Piper JW,Swerlick RA,Zhu C

更新日期：1998-01-01 00:00:00

abstract：:A complete mechanistic understanding of membrane-localized processes in vesicular transport, such as membrane budding and fission, requires their reconstitution with biochemically-defined components from a biochemically-defined substrate. Supported bilayers formed by vesicle fusion represent an attractive substrate fo...

journal_title：Biophysical journal

authors： Pucadyil TJ,Schmid SL

更新日期：2010-07-21 00:00:00

abstract：:A novel way to model permeation through ionic channels is formulated. Our method does not require that equilibrium exists in the channel or at the channel interfaces. In addition, the potential profile does not need to be specified and the assumption of constant field across the membrane does not need to be made. Our ...

journal_title：Biophysical journal

authors： Skinner FK,Ward CA,Bardakjian BL

更新日期：1993-08-01 00:00:00

abstract：:A new method is presented to measure local cytoplasmic deformation and rate of deformation in motile active neutrophils. The deformation is expressed in terms of biomechanical strains and strain rates. For this purpose small phagocytosed latex microspheres were used as intracellular markers. Planar Lagrangian and Eule...

journal_title：Biophysical journal

authors： Simon SI,Schmid-Schönbein GW

更新日期：1990-08-01 00:00:00

abstract：:A 10-ns molecular dynamics simulation of mouse acetylcholinesterase was analyzed, with special attention paid to the fluctuation in the width of the gorge and opening events of the back door. The trajectory was first verified to ensure its stability. We defined the gorge proper radius as the measure for the extent of ...

journal_title：Biophysical journal

authors： Tai K,Shen T,Börjesson U,Philippopoulos M,McCammon JA

更新日期：2001-08-01 00:00:00

abstract：:Slow fluorescence transients in Chlamydomonas reinhardi arise after transitions from high light intensities to low light or dark conditions. Characteristics of the newly described transient phenomena include: (a) A slow biphasic decrease in fluorescence yield occurs in the dark, followed by an even slower, hour long, ...

journal_title：Biophysical journal

authors： Rice GE,Bannister TT

更新日期：1980-11-01 00:00:00

abstract：:An equation is derived, which connects two functions P(r) and g(x), the first being related to the scattering intensity by a simple transformation, the second to the elctron density distribution of a spherically symmetric structure. This relation seems to be a suitable starting point for an analysis of shell structure...

journal_title：Biophysical journal

authors： Weick D

更新日期：1974-03-01 00:00:00

abstract：:In the intestinal epithelium, proliferated epithelial cells ascend the crypts and villi and shed at the villus tips into the gut lumen. In this study, we theoretically investigate the roles of the villi on cell turnover. We present a stochastic model that focuses on the duration over which cells migrate the shortest p...

journal_title：Biophysical journal

authors： Kai Y

更新日期：2021-01-13 00:00:00

abstract：:Protein conformational fluctuations modulate the catalytic powers of enzymes. The frequency of conformational fluctuations may modulate the catalytic rate at individual reaction steps. In this study, we modulated the rotary fluctuation frequency of F1-ATPase (F1) by attaching probes with different viscous drag coeffic...

journal_title：Biophysical journal

authors： Watanabe R,Hayashi K,Ueno H,Noji H

更新日期：2013-11-19 00:00:00

abstract：:Electric field pulses have been reported to induce long-lived permeabilization and fusogenicity on cell membranes. The two membrane property alterations are under the control of the field strength, the pulse duration, and the number of pulses. Experiments on mammalian cells pulsed by square wave form pulses and then b...

journal_title：Biophysical journal

authors： Teissié J,Ramos C

更新日期：1998-04-01 00:00:00

abstract：:Neuronal synapses are highly specialized structures for communication between nerve cells. Knowledge about their molecular organization and dynamics is still incomplete. The large multidomain protein Bassoon plays a major role in scaffolding and organizing the cytomatrix at the active zone of neurotransmitter release ...

journal_title：Biophysical journal

authors： Jose M,Nair DK,Altrock WD,Dresbach T,Gundelfinger ED,Zuschratter W

更新日期：2008-02-15 00:00:00

abstract：:Density-gradient centrifugation of disintegrated cells from a variety of plants gave two kinds of chlorophyll particles from all except the blue-green algae. As in previous procedures using detergents, the lighter Fraction 1 particles usually had greater absorption at longer wavelengths; they always had a lower ratio ...

journal_title：Biophysical journal

authors： Brown JS

更新日期：1969-12-01 00:00:00

abstract：:The heterologous expression and purification of membrane proteins represent major limitations for their functional and structural analysis. Here we describe a new method of incorporation of transmembrane proteins in planar lipid bilayer starting from 1 pmol of solubilized proteins. The principle relies on the direct i...

journal_title：Biophysical journal

authors： Milhiet PE,Gubellini F,Berquand A,Dosset P,Rigaud JL,Le Grimellec C,Lévy D

更新日期：2006-11-01 00:00:00