A Dynamic Biochemomechanical Model of Geometry-Confined Cell Spreading.

abstract：:Perfusion is one of the most important processes maintaining organ health. From a computational perspective, however, perfusion is among the least-studied physiological processes of the heart. The recent development of novel nanoparticle-based targeted cardiac therapy calls for novel simulation methods that can provid...

journal_title：Biophysical journal

authors： Diem AK,Valen-Sendstad K

更新日期：2019-12-17 00:00:00

abstract：:mRNAs are involved in complicated supramolecular complexes with human 40S and 80S ribosomes responsible for the protein synthesis. In this work, a derivative of nonaribonucleotide pUUCGUAAAA with nitroxide spin labels attached to the 5'-phosphate and to the C8 atom of the adenosine in sixth position (mRNA analog) was ...

journal_title：Biophysical journal

authors： Malygin AA,Graifer DM,Meschaninova MI,Venyaminova AG,Krumkacheva OA,Fedin MV,Karpova GG,Bagryanskaya EG

更新日期：2015-12-15 00:00:00

abstract：:A new Fourier series representation of supercoiled DNA is employed in Langevin dynamics simulations to study large-scale configurational motions of intermediate-length chains. The polymer is modeled as an ideal elastic rod subject to long-range van der Waals' interactions. The van der Waals' term prevents the self-con...

journal_title：Biophysical journal

authors： Liu G,Schlick T,Olson AJ,Olson WK

更新日期：1997-10-01 00:00:00

abstract：:We have attempted to link the solution actomyosin ATPase with the mechanical properties of in vitro actin filament sliding over heavy meromyosin. To accomplish this we perturbed the system by altering the substrate with various NTPs and divalent cations, and by altering ionic strength. A wide variety of enzymatic and ...

journal_title：Biophysical journal

authors： Amitani I,Sakamoto T,Ando T

更新日期：2001-01-01 00:00:00

abstract：:We studied the photocurrents of a cyanobacterial rhodopsin Gloeobacter violaceus (GR) in Xenopus laevis oocytes and HEK-293 cells. This protein is a light-driven proton pump with striking similarities to marine proteorhodopsins, including the D121-H87 cluster of the retinal Schiff base counterion and a glutamate at po...

journal_title：Biophysical journal

authors： Vogt A,Wietek J,Hegemann P

更新日期：2013-11-05 00:00:00

abstract：:Experiments by others have used isolated cell or bilayer membrane preparations to study the dramatic phenomena associated with electroporation. The present study observes electroporation behavior in an intact tissue. Viable samples of frog skin (Rana pipiens) were exposed to short electrical pulses of varying width an...

journal_title：Biophysical journal

authors： Powell KT,Morgenthaler AW,Weaver JC

更新日期：1989-12-01 00:00:00

abstract：:Dielectrophoresis and electrorotation are commonly used to measure dielectric properties and membrane electrical parameters of biological cells. We have derived quantitative relationships for several critical points, defined in Fig. A 1, which characterize the dielectrophoretic spectrum and the electrorotational spect...

journal_title：Biophysical journal

authors： Gimsa J,Marszalek P,Loewe U,Tsong TY

更新日期：1991-10-01 00:00:00

abstract：:The effects of Mg++ on the spatial organization of nuclei from rat hepatocytes are analyzed in the range 0-60 mM, in the presence of suitable concentrations of KCl to reproduce physiological conditions. It is shown that the scatter-signal distribution measured by means of a flow microfluorimeter is greatly affected by...

journal_title：Biophysical journal

authors： Grattarola M,Carlo P,Giannetti G,Finollo R,Viviani R,Chiabrera A

更新日期：1985-04-01 00:00:00

abstract：:We present a combined experimental and computational study of the bundling of F-actin filaments induced by lysozyme proteins. Synchrotron small-angle x-ray scattering results show that these bundles consist of close-packed columnar complexes in which the actin is held together by incommensurate, one-dimensional arrays...

journal_title：Biophysical journal

authors： Guáqueta C,Sanders LK,Wong GC,Luijten E

更新日期：2006-06-15 00:00:00

abstract：:The recent work is surveyed which leads to the suggestions that the conformation of globular proteins in solution corresponds to a dynamic ensemble of rapidly interconverting spatial structures, that clusters of hydrophobic amino acid side chains have an important role in the architecture of protein molecules, and tha...

journal_title：Biophysical journal

authors： Wüthrich K,Wagner G,Richarz R,Braun W

更新日期：1980-10-01 00:00:00

abstract：:The neutral anesthetics chloroform and benzyl alcohol, at concentrations that block the nerve impulse, greatly modify the transport parameters of positive and negative ions in lipid bilayers made from monolayers. Both chloroform and benzyl alcohol increase the membrane permeability to these ions and increase the trans...

journal_title：Biophysical journal

authors： Reyes J,Latorre R

更新日期：1979-11-01 00:00:00

abstract：:A detailed picture of water and ion properties in small pores is important for understanding the behavior of biological ion channels. Several recent modeling studies have shown that small, hydrophobic pores exclude water and ions even if they are physically large enough to accommodate them, a mechanism called hydropho...

journal_title：Biophysical journal

authors： Spronk SA,Elmore DE,Dougherty DA

更新日期：2006-05-15 00:00:00

abstract：:The refractive indices, water content, and volume of wet-spun films of Na-hyaluronate have been measured as a function of relative humidity (rh). These data are used with the Lorentz-Lorenz formula to determine the optical polarizabilities of Na-hyaluronate parallel and perpendicular to the helical axis. The analysis ...

journal_title：Biophysical journal

authors： Flowers MR,Marlow RL,Lee SA,Lavalle N,Rupprecht A

更新日期：1992-08-01 00:00:00

abstract：:It has been hypothesized, from earlier studies on single-tether extraction from individual leukocytes and human umbilical vein endothelial cells, that during rolling of leukocytes on the endothelium, simultaneous extraction of membrane nanotubes (tethers) occurs, resulting in enhancement of the force decrease on the a...

journal_title：Biophysical journal

authors： Girdhar G,Shao JY

更新日期：2007-12-01 00:00:00

abstract：:Orientation angles of five emitting dipoles of chlorophyll a in thylakoids were estimated from low temperature fluorescence polarization ratio spectra of magnetically oriented chloroplasts. A simple expression is given also for the evaluation of data from linear dichroism measurements. It is shown that the Qy dipoles ...

journal_title：Biophysical journal

authors： Garab GI,Kiss JG,Mustárdy LA,Michel-Villaz M

更新日期：1981-06-01 00:00:00

abstract：:Solubility plays a major role in protein purification, and has serious implications in many diseases. We studied the effects of pH and mutations on protein solubility by calculating the transfer free energy from the condensed phase to the solution phase. The condensed phase was modeled as an implicit solvent, with a d...

journal_title：Biophysical journal

authors： Tjong H,Zhou HX

更新日期：2008-09-15 00:00:00

abstract：:The mechanism of high-voltage pulse-induced permeabilization of the stratum corneum, the outer layer of the skin, is still not completely understood. It has been suggested that joule heating resulting from the applied pulse may play a major role in disrupting the stratum corneum. In this study, electrical and ultrastr...

journal_title：Biophysical journal

authors： Gallo SA,Sen A,Hensen ML,Hui SW

更新日期：2002-01-01 00:00:00

abstract：:Fatty acid binding proteins play an important role in the transportation of fatty acids. Despite intensive studies, how fatty acids enter the protein cavity for binding is still controversial. Here, a gap-closed variant of human intestinal fatty acid binding protein was generated by mutagenesis, in which the gap is lo...

journal_title：Biophysical journal

authors： Xiao T,Lu Y,Fan JS,Yang D

更新日期：2020-01-21 00:00:00

abstract：:Aerotaxis is a particular form of "energy taxis". It is based on a largely elusive signal transduction machinery. In aerotaxis, oxygen dissolved in water plays the role of both attractant (at moderate concentrations) and repellent (at high and low concentrations). Cells swimming from favorable oxygen concentrations in...

journal_title：Biophysical journal

authors： Mazzag BC,Zhulin IB,Mogilner A

更新日期：2003-12-01 00:00:00

abstract：:The intrinsic birefringences of TMV, tropocollagen, and paramyosin were calculated from flow birefringence measurements using the theory of Peterlin and Stuart. The values are -0.029, -0.029, and -0.030, respectively. The intrinsic birefringences of TMV and tropocollagen were measured as a function of the refractive i...

journal_title：Biophysical journal

authors： TAYLOR EW,CRAMER W

更新日期：1963-03-01 00:00:00

abstract：:Characterizing the ionic distribution around chromatin is important for understanding the electrostatic forces governing chromatin structure and function. Here we develop an electrostatic model to handle multivalent ions and compute the ionic distribution around a mesoscale chromatin model as a function of conformatio...

journal_title：Biophysical journal

authors： Gan HH,Schlick T

更新日期：2010-10-20 00:00:00

abstract：:The triplet state absorption and phosphorescence of Zn and Pd derivatives of myoglobin were compared. Both metal derivatives exhibit long triplet state lifetimes at room temperature, but whereas the Pd derivative showed exponential decay and an isosbestic point in the transient absorption spectra, the decay of the Zn ...

journal_title：Biophysical journal

authors： Papp S,Vanderkooi JM,Owen CS,Holtom GR,Phillips CM

更新日期：1990-07-01 00:00:00

abstract：:By combining a description of the coupled solute and water flows through the in vivo cornea with a set of appropriate mechanical equilibrium conditions, it is possible to calculate directly the corneal thickness, given the corneal temperature, the state of the aqueous and tears, the swelling pressure-hydration relatio...

journal_title：Biophysical journal

authors： Friedman MH

更新日期：1972-06-01 00:00:00

abstract：:The use of the long-lived fluorescence probe coronene (mean value of tau(FL) approximately 200 ns) is described for investigating submicrosecond lipid dynamics in DPPC model bilayer systems occurring below the lipid phase transition. Time-resolved fluorescence emission anisotropy decay profiles, measures as a function...

journal_title：Biophysical journal

authors： Davenport L,Targowski P

更新日期：1996-10-01 00:00:00

abstract：:Based on differences between the x-ray crystal structures of ligand-bound and unbound forms, the activation of the erythropoietin receptor (EPOR) was initially proposed to involve a cross-action scissorlike motion. However, the validity of the motions involved in the scissorlike model has been recently challenged. Her...

journal_title：Biophysical journal

authors： Corbett MSP,Mark AE,Poger D

更新日期：2017-02-28 00:00:00

abstract：:The recently developed method of site-directed Fourier transform infrared dichroism for obtaining orientational constraints of oriented polymers is applied here to the transmembrane domain of the vpu protein from the human immunodeficiency virus type 1 (HIV-1). The infrared spectra of the 31-residue-long vpu peptide r...

journal_title：Biophysical journal

authors： Kukol A,Arkin IT

更新日期：1999-09-01 00:00:00

abstract：:The shapes of fluorescence induction curves in spinach chloroplasts, measured using double-flash pump-probe techniques, are shown to depend on the duration of the actinic flashes. For flash durations tau(0)

journal_title：Biophysical journal

authors： Valkunas L,Geacintov NE,France L,Breton J

更新日期：1991-02-01 00:00:00

abstract：:Theoretical studies of the electronic structure and spectra of models for the ferric resting state and Compound I intermediates of horseradish peroxidase (HRP-I) and catalase (CAT-I) have been performed using the INDO-RHF/CI method. The goals of these studies were twofold: i) to determine whether the axial ligand of H...

journal_title：Biophysical journal

authors： Du P,Loew GH

更新日期：1995-01-01 00:00:00

abstract：:Many aspects of chromatin biology are influenced by the nuclear compartment in which a locus resides, from transcriptional regulation to DNA repair. Further, the dynamic and variable localization of a particular locus across cell populations and over time makes analysis of a large number of cells critical. As a conseq...

journal_title：Biophysical journal

authors： Zhao Y,Schreiner SM,Koo PK,Colombi P,King MC,Mochrie SG

更新日期：2016-07-12 00:00:00

abstract：:We report here a method for the determination of the pKa of histidine in complex or heterogeneous systems amenable to neither solid-state nor solution NMR spectroscopy. Careful synthesis of a fluorenylmethyloxycarbonyl- and trityl-protected, C2-deuterated histidine produces a vibrational-probe-equipped amino acid that...

journal_title：Biophysical journal

authors： Braun GA,Pogostin BH,Pucetaite M,Londergan CH,Åkerfeldt KS

更新日期：2020-11-03 00:00:00