Abstract:
:Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Hwu Y,Tsai WL,Chang HM,Yeh HI,Hsu PC,Yang YC,Su YT,Tsai HL,Chow GM,Ho PC,Li SC,Moser HO,Yang P,Seol SK,Kim CC,Je JH,Stefanekova E,Groso A,Margaritondo Gdoi
10.1529/biophysj.103.034991subject
Has Abstractpub_date
2004-12-01 00:00:00pages
4180-7issue
6eissn
0006-3495issn
1542-0086pii
S0006-3495(04)73882-5journal_volume
87pub_type
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