Abstract:
:To assay cell cycle progression in synchronized culture of yeast we have applied dielectric spectroscopy to its real-time monitoring. The dielectric monitoring is based on the electromagnetic induction method, regarded as a nonelectrode method, which has resolved the problems encountered in measurements with metal electrodes, namely electrode polarization and bubble formation on electrodes. In the synchronized culture with temperature-sensitive cell division cycle mutants, the permittivity of the culture broth showed cyclic changes at frequencies below 300 kHz. The increase and decrease in the cyclic changes of the relative permittivity correspond to the increase in cell length and bud size and to the septum formation between mother and daughter cells, respectively.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Asami K,Gheorghiu E,Yonezawa Tdoi
10.1016/S0006-3495(99)77487-4subject
Has Abstractpub_date
1999-06-01 00:00:00pages
3345-8issue
6eissn
0006-3495issn
1542-0086pii
S0006-3495(99)77487-4journal_volume
76pub_type
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