Abstract:
:Crystals have been grown of "sperm whale" myoglobin produced in Escherichia coli from a synthetic gene and the structure has been solved to 1.9 A resolution. Because of a remaining initiator methionine, this protein crystallizes in a different space group from native sperm whale myoglobin. The three-dimensional structure of the synthetic protein is essentially identical to the native sperm whale protein. However, the crystallographic B-factors for parts of the molecule are quite different in the two crystal forms, and provide a measure of the effect of different packing constraints on the flexibility of the protein. The effect of the packing forces is to reduce the mobility of the protein in the regions of contact and thereby introduce differences in mobilities between the two crystal forms. Discrepancies between mobilities calculated from molecular dynamics simulations and crystallography can be reduced by considering the data from both crystal forms.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Phillips GN Jrdoi
10.1016/S0006-3495(90)82540-6subject
Has Abstractpub_date
1990-02-01 00:00:00pages
381-3issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(90)82540-6journal_volume
57pub_type
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2018-05-22 00:00:00
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journal_title:Biophysical journal
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更新日期:2018-08-21 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.094425
更新日期:2007-06-15 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1988-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-05-09 00:00:00
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pub_type: 杂志文章
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更新日期:1971-12-01 00:00:00
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更新日期:2003-11-01 00:00:00
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pub_type: 信件
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更新日期:2006-02-15 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/S0006-3495(86)83454-3
更新日期:1986-08-01 00:00:00
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更新日期:2018-11-06 00:00:00
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更新日期:2017-11-07 00:00:00
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更新日期:1988-11-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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更新日期:2003-03-01 00:00:00
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更新日期:2014-03-04 00:00:00
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pub_type: 杂志文章
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更新日期:2011-11-16 00:00:00
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更新日期:2002-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00