Asymmetric Phosphatidylethanolamine Distribution Controls Fusion Pore Lifetime and Probability.

abstract：:Late-onset heart failure (HF) is a known side effect of doxorubicin chemotherapy. Typically, patients are diagnosed when already at an irreversible stage of HF, which allows few or no treatment options. Identifying the causes of compromised cardiac function in this patient group may improve early patient diagnosis and...

journal_title：Biophysical journal

authors： Lewalle A,Land S,Merken JJ,Raafs A,Sepúlveda P,Heymans S,Kleinjans J,Niederer SA

更新日期：2019-12-17 00:00:00

abstract：:Epithelial injury induces rapid recruitment of antimicrobial leukocytes to the wound site. In zebrafish larvae, activation of the epithelial NADPH oxidase Duox at the wound margin is required early during this response. Before injury, leukocytes are near the vascular region, that is, ∼100-300 μm away from the injury s...

journal_title：Biophysical journal

authors： Jelcic M,Enyedi B,Xavier JB,Niethammer P

更新日期：2017-05-09 00:00:00

abstract：:The effect of S-methylating cysteine-102 (cys-102) (SH----SSCH3) of yeast isozyme-1 (iso-1) ferricytochrome c has been studied using proton NMR spectroscopy. COSY, NOESY, and one-dimensional nuclear Overhauser effect (NOE) difference spectroscopies have all been used. The NMR spectrum of this derivative is very simila...

journal_title：Biophysical journal

authors： Busse SC,Moench SJ,Satterlee JD

更新日期：1990-07-01 00:00:00

abstract：:Crystals have been grown of "sperm whale" myoglobin produced in Escherichia coli from a synthetic gene and the structure has been solved to 1.9 A resolution. Because of a remaining initiator methionine, this protein crystallizes in a different space group from native sperm whale myoglobin. The three-dimensional struct...

journal_title：Biophysical journal

authors： Phillips GN Jr

更新日期：1990-02-01 00:00:00

abstract：:We introduce here a novel Monte Carlo simulation method for studying the interactions of hydrophobic peptides with lipid membranes. Each of the peptide's amino acids is represented as two interaction sites: one corresponding to the backbone alpha-carbon and the other to the side chain, with the membrane represented as...

journal_title：Biophysical journal

authors： Kessel A,Shental-Bechor D,Haliloglu T,Ben-Tal N

更新日期：2003-12-01 00:00:00

abstract：:The plasma membrane (PM) serves as the functional interface between a cell and its environment, hosting extracellular signal transduction and nutrient transport among a variety of other processes. To support this extensive functionality, PMs are organized into lateral domains, including ordered, lipid-driven assemblie...

journal_title：Biophysical journal

authors： Levental KR,Lorent JH,Lin X,Skinkle AD,Surma MA,Stockenbojer EA,Gorfe AA,Levental I

更新日期：2016-04-26 00:00:00

abstract：:Recent development of titratable coions has paved the way for realizing all-atom molecular dynamics at constant pH. To further improve physical realism, here we describe a technique in which proton titration of the solute is directly coupled to the interconversion between water and hydroxide or hydronium. We test the ...

journal_title：Biophysical journal

authors： Chen W,Wallace JA,Yue Z,Shen JK

更新日期：2013-08-20 00:00:00

abstract：:Employing nonequilibrium molecular dynamics simulations, a comprehensive computational study of the photoinduced conformational dynamics of a photoswitchable bicyclic azobenzene octapeptide is presented. The calculation of time-dependent probability distributions along various global and local reaction coordinates rev...

journal_title：Biophysical journal

authors： Nguyen PH,Gorbunov RD,Stock G

更新日期：2006-08-15 00:00:00

abstract：:Choice of network topology for gene regulation has been a question of interest for a long time. How do simple and more complex topologies arise? In this work, we analyze the topology of the marRAB operon in Escherichia coli, which is associated with control of expression of genes associated with conferring resistance ...

journal_title：Biophysical journal

authors： Prajapat MK,Jain K,Saini S

更新日期：2015-10-06 00:00:00

abstract：:Ceramides have been implicated in the initiation of apoptosis by permeabilizing the mitochondrial outer membrane to small proteins, including cytochrome c. In addition, ceramides were shown to form large metastable channels in planar membranes and liposomes, indicating that these lipids permeabilize membranes directly...

journal_title：Biophysical journal

authors： Anishkin A,Sukharev S,Colombini M

更新日期：2006-04-01 00:00:00

abstract：:The interaction of the nonionic surfactant dodecylmaltoside (DM) with phosphatidylcholine liposomes was investigated. Permeability alterations were detected as a change in 5(6)-carboxyfluorescein released from the interior of vesicles and bilayer solubilization as a decrease in the static light scattered by liposome s...

journal_title：Biophysical journal

authors： de la Maza A,Parra JL

更新日期：1997-04-01 00:00:00

abstract：:During the first 2 hours of Drosophila development, precisely orchestrated nuclear cleavages, cytoskeletal rearrangements, and directed membrane growth lead to the formation of an epithelial sheet around the yolk. The newly formed epithelium remains relatively quiescent during the next hour as it is patterned by mater...

journal_title：Biophysical journal

authors： Dutta S,Djabrayan NJ,Smits CM,Rowley CW,Shvartsman SY

更新日期：2020-05-19 00:00:00

abstract：:The effect of uncharged, dipolar phloretin on anion and cation conductance through a black lipid membrane can be used to study its adsorption behavior. The adsorption of phloretin can be described by a Langmuir isotherm with weak dipole-dipole interaction. ...

journal_title：Biophysical journal

authors： de Levie R,Rangarajan SK,Seelig PF,Andersen OS

更新日期：1979-02-01 00:00:00

abstract：:The interactions between phospholipid molecules in suspensions have been studied by using mass spectrometry. Electrospray mass spectra of homogeneous preparations formed from three different phospholipid molecules demonstrate that under certain conditions interactions between 90 and 100 lipid molecules can be preserve...

journal_title：Biophysical journal

authors： Hanson CL,Ilag LL,Malo J,Hatters DM,Howlett GJ,Robinson CV

更新日期：2003-12-01 00:00:00

abstract：:Probabilities of disorder for FlgM proteins of 39 species whose optimal growth temperature ranges from 273 K (0°C) to 368 K (95°C) were predicted by a newly developed method called Sequence-based Prediction with Integrated NEural networks for Disorder (SPINE-D). We showed that the temperature-dependent behavior of Flg...

journal_title：Biophysical journal

authors： Wang J,Yang Y,Cao Z,Li Z,Zhao H,Zhou Y

更新日期：2013-12-03 00:00:00

abstract：:FKBP12 was removed from ryanodine receptors (RyRs) by incubation of rabbit skeletal muscle terminal cisternae membranes with rapamycin. The extent of FKBP12 removal was estimated by immunostaining Western blots of terminal cisternae proteins. Single FKBP12-depleted RyR channels, incorporated into planar lipid bilayers...

journal_title：Biophysical journal

authors： Ahern GP,Junankar PR,Dulhunty AF

更新日期：1997-01-01 00:00:00

abstract：:Although actin and myosin are important contributors to cell-force generation, shape change, and motility, their contributions to cell stiffness and frequency-dependent rheology have not been conclusively determined. We apply several pharmacological interventions to cultured epithelial cells to elucidate the roles of ...

journal_title：Biophysical journal

authors： Van Citters KM,Hoffman BD,Massiera G,Crocker JC

更新日期：2006-11-15 00:00:00

abstract：:The cell membrane of Salinibacter ruber contains xanthorhodopsin, a light-driven transmembrane proton pump with two chromophores: a retinal and the carotenoid, salinixanthin. Action spectra for transport had indicated that light absorbed by either is utilized for function. If the carotenoid is an antenna in this prote...

journal_title：Biophysical journal

authors： Balashov SP,Imasheva ES,Wang JM,Lanyi JK

更新日期：2008-09-01 00:00:00

abstract：:Quantitative analysis of DNA microarray data is complicated by uncertainties inherent to the experimental setup. Using computer simulations and real-time experimental results, we have previously demonstrated effects of multiplex reactions on a single sensing zone of an array, which may be a leading factor in erroneous...

journal_title：Biophysical journal

authors： Bishop J,Chagovetz AM,Blair S

更新日期：2008-03-01 00:00:00

abstract：:A kinetic model of the effect of agonist and anesthetics on ligand-gated ion channels, developed in earlier work, is further refined and used to predict traces observed in fast-perfusion electrophysiological studies on recombinant GABAA receptors under a wide range of agonist and/or anesthetic concentrations. The mode...

journal_title：Biophysical journal

authors： Lee DK,Albershardt DJ,Cantor RS

更新日期：2015-03-10 00:00:00

abstract：:An alternative theoretical approach to enzyme kinetics that is particularly applicable to single-molecule enzymology is presented. The theory, originated by Van Slyke and Cullen in 1914, develops enzyme kinetics from a "time perspective" rather than the traditional "rate perspective" and emphasizes the nonequilibrium ...

journal_title：Biophysical journal

authors： Qian H

更新日期：2008-07-01 00:00:00

abstract：:Recent experimental studies of the role of microtubule sliding in neurite outgrowth suggested a qualitative model, according to which kinesin-1 motors push the minus-end-out microtubules against the cell membrane and generate the early cell processes. At the later stage, dynein takes over the sliding, expels the minus...

journal_title：Biophysical journal

authors： Oelz DB,Del Castillo U,Gelfand VI,Mogilner A

更新日期：2018-10-16 00:00:00

abstract：:In the studies of force-induced conformational transitions of biomolecules, the large timescale difference from experiments presents the challenge of obtaining convergent sampling for molecular dynamics simulations. To circumvent this fundamental problem, an approach combining the replica-exchange method and umbrella ...

journal_title：Biophysical journal

authors： Zeng X,Hu H,Zhou HX,Marszalek PE,Yang W

更新日期：2010-02-17 00:00:00

abstract：:Biological systems often have to measure extremely low concentrations of chemicals with high precision. When dealing with such small numbers of molecules, the inevitable randomness of physical transport processes and binding reactions will limit the precision with which measurements can be made. An important question ...

journal_title：Biophysical journal

authors： Kaizu K,de Ronde W,Paijmans J,Takahashi K,Tostevin F,ten Wolde PR

更新日期：2014-02-18 00:00:00

abstract：:The spatiotemporal organization and dynamics of the plasma membrane and its constituents are central to cellular function. Fluorescence-based single-particle tracking has emerged as a powerful approach for studying the single molecule behavior of plasma-membrane-associated events because of its excellent background su...

journal_title：Biophysical journal

authors： de Wit G,Albrecht D,Ewers H,Kukura P

更新日期：2018-06-19 00:00:00

abstract：:Force spectroscopy is commonly used to measure the kinetics of processes occurring in single biological molecules. These measurements involve attaching the molecule of interest to micron-sized or larger force probes via compliant linkers. Recent theoretical work has described how the properties of the probes and linke...

journal_title：Biophysical journal

authors： Neupane K,Woodside MT

更新日期：2016-07-26 00:00:00

abstract：:Herein, we identify the coordination environment of Cu²(+) in the human α1-glycine receptor (GlyR). GlyRs are members of the pentameric ligand-gated ion channel superfamily (pLGIC) that mediate fast signaling at synapses. Metal ions like Zn²(+) and Cu²(+) significantly modulate the activity of pLGICs, and metal ion co...

journal_title：Biophysical journal

authors： Ruthstein S,Stone KM,Cunningham TF,Ji M,Cascio M,Saxena S

更新日期：2010-10-20 00:00:00

abstract：:Huntington's disease is a heritable neurodegenerative disease that is caused by a CAG expansion in the first exon of the huntingtin gene. This expansion results in an elongated polyglutamine domain that increases the propensity of huntingtin exon-1 to form cross-β fibrils. Although the polyglutamine domain is importan...

journal_title：Biophysical journal

authors： Falk AS,Bravo-Arredondo JM,Varkey J,Pacheco S,Langen R,Siemer AB

更新日期：2020-11-17 00:00:00

abstract：:Skeletal muscle contraction is a canonical example of motor-driven force generation. Despite the long history of research in this topic, a mechanistic explanation of the collective myosin force generation is lacking. We present a theoretical model of muscle contraction based on the conformational movements of individu...

journal_title：Biophysical journal

authors： Lan G,Sun SX

更新日期：2005-06-01 00:00:00

abstract：:We have refined the structure of the DNA Three-Way Junction complex, TWJ-TC, described in the companion paper by quantitative analysis of two 2D NOESY spectra (mixing times 60 and 200 ms) obtained in D2O solution. NOESY crosspeak intensities extracted from these spectra were used in two kinds of refinement procedure: ...

journal_title：Biophysical journal

authors： Ouporov IV,Leontis NB

更新日期：1995-01-01 00:00:00