Routes to DNA accessibility: alternative pathways for nucleosome unwinding.

abstract：:In the intestinal epithelium, proliferated epithelial cells ascend the crypts and villi and shed at the villus tips into the gut lumen. In this study, we theoretically investigate the roles of the villi on cell turnover. We present a stochastic model that focuses on the duration over which cells migrate the shortest p...

journal_title：Biophysical journal

authors： Kai Y

更新日期：2021-01-13 00:00:00

abstract：:The process of clathrin self-assembly into closed shells with the Fullerene structure is investigated. It is argued that the shell size distribution is governed by the kinetics of assembly and depends on the rate of growth controlled by the free clathrin concentration. The particularly abundant small structures--the "...

journal_title：Biophysical journal

authors： Shraiman BI

更新日期：1997-02-01 00:00:00

abstract：:Elastin-derived peptides are released from the extracellular matrix remodeling by numerous proteases and seem to regulate many biological processes, notably cancer progression. The canonical elastin peptide is VGVAPG, which harbors the XGXXPG consensus pattern, allowing interaction with the elastin receptor complex lo...

journal_title：Biophysical journal

authors： Hernández B,Crowet JM,Thiery J,Kruglik SG,Belloy N,Baud S,Dauchez M,Debelle L

更新日期：2020-06-02 00:00:00

abstract：:How do Ca channels conduct Ca ions during the cardiac action potential? We attempt to answer this question by applying a two-microelectrode technique, previously used for Na and K currents, in which we record the patch current and the action potential at the same time (Mazzanti, M., and L. J. DeFelice. 1987. Biophys. ...

journal_title：Biophysical journal

authors： Mazzanti M,DeFelice LJ

更新日期：1990-10-01 00:00:00

abstract：:In a contracting muscle, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containing) filaments during cyclical ATP-driven interactions toward the center of the sarcomere, the structural unit of striated muscle. Cross-bridge attachments in the sarcomere have been reported to exh...

journal_title：Biophysical journal

authors： Linari M,Piazzesi G,Pertici I,Dantzig JA,Goldman YE,Lombardi V

更新日期：2020-03-10 00:00:00

abstract：:Human posttranslationally modified N-ras oncogenes are known to be implicated in numerous human cancers. Here, we applied a combination of experimental and computational techniques to determine structural and dynamical details of the lipid chain modifications of an N-ras heptapeptide in 1,2-dimyristoyl-sn-glycero-3-ph...

journal_title：Biophysical journal

authors： Vogel A,Tan KT,Waldmann H,Feller SE,Brown MF,Huster D

更新日期：2007-10-15 00:00:00

abstract：:Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique. Spatially resolved fluorescence decays from single mitochondria or only a very few organelles o...

journal_title：Biophysical journal

authors： Ramadass R,Bereiter-Hahn J

更新日期：2008-10-01 00:00:00

abstract：:A free radical probe was introduced into single barnacle muscle cells, and its freedom of motion inferred from the spin resonance spectra. The probe reported an average local viscosity of 5-10 cp compared with 1 cp for pure water. From a comparison of the temperature dependence of the probe's tumbling rate in model aq...

journal_title：Biophysical journal

authors： Sachs F,Latorre R

更新日期：1974-04-01 00:00:00

abstract：:The folding of short alanine-based peptides with different numbers of lysine residues is simulated at constant temperature (274 K) using the rigid-element Monte Carlo method. The solvent-referenced potential has prevented the multiple-minima problem in helix folding. From various initial structures, the peptides with ...

journal_title：Biophysical journal

authors： Sung SS

更新日期：1995-03-01 00:00:00

abstract：:In striated muscle thin filament activation is initiated by Ca(2+) binding to troponin C and augmented by strong myosin binding to actin (cross-bridge formation). Several lines of evidence have led us to hypothesize that thin filament properties may limit the level and rate of force development in cardiac muscle at al...

journal_title：Biophysical journal

authors： Regnier M,Martin H,Barsotti RJ,Rivera AJ,Martyn DA,Clemmens E

更新日期：2004-09-01 00:00:00

abstract：:It is now known that the flexibility of the troponin T (TnT) tail determines thin filament conformation and hence cross-bridge cycling properties, expanding the classic structural role of TnT to a dynamic role regulating sarcomere function. Here, using transgenic mice bearing R-92W and R-92L missense mutations in card...

journal_title：Biophysical journal

authors： He H,Javadpour MM,Latif F,Tardiff JC,Ingwall JS

更新日期：2007-09-01 00:00:00

abstract：:The addition of 10(-3) M p-chloromercuribenzene sulfonate (PCMBS) to the solution bathing the mucosal surface of rabbit colon has no effect on the rate of active Na transport but blocks or reverses the inhibitory action of amiloride. The tissue must be exposed to PCMBS for 20-30 min for a complete blocking effect, and...

journal_title：Biophysical journal

authors： Gottlieb GP,Turnheim K,Frizzell RA,Schultz SG

更新日期：1978-04-01 00:00:00

abstract：:The structure of the Bowman-Birk inhibitor from Vigna unguiculata seeds (BTCI) in complex with beta-trypsin was solved and refined at 1.55 A to a crystallographic R(factor) of 0.154 and R(free) of 0.169, and represents the highest resolution for a Bowman-Birk inhibitor structure to date. The BTCI-trypsin interface is ...

journal_title：Biophysical journal

authors： Barbosa JA,Silva LP,Teles RC,Esteves GF,Azevedo RB,Ventura MM,de Freitas SM

更新日期：2007-03-01 00:00:00

abstract：:Stationary and kinetic experiments were performed on lipid bilayer membranes to study the mechanism of iodine- and bromine-mediated halide transport in detail. The stationary conductance data suggested that four different 1:1 complexes between I2 and Br2 and the halides I- and Br- were responsible for the observed con...

journal_title：Biophysical journal

authors： Klotz KH,Benz R

更新日期：1993-12-01 00:00:00

abstract：:The space-clamped squid axon membrane and two versions of the Hodgkin-Huxley model (the original, and a strongly adapting version) are subjected to a first order dynamic analysis. Stable, repetitive firing is induced by phase-locking nerve impulses to sinusoidal currents. The entrained impulses are then pulse position...

journal_title：Biophysical journal

authors： Fohlmeister JF,Adelman WJ Jr,Poppele RE

更新日期：1980-04-01 00:00:00

abstract：:Tendons are important load-bearing structures, which are frequently injured in both sports and work. Type I collagen fibrils are the primary components of tendons and carry most of the mechanical loads experienced by the tissue, however, knowledge of how load is transmitted between and within fibrils is limited. The p...

journal_title：Biophysical journal

authors： Svensson RB,Mulder H,Kovanen V,Magnusson SP

更新日期：2013-06-04 00:00:00

abstract：:In the photocycle of bacteriorhodopsin at pH 7, a proton is ejected to the extracellular medium during the protonation of Asp-85 upon formation of the M intermediate. The group that releases the ejected proton does not become reprotonated until the prephotolysis state is restored from the N and O intermediates. In con...

journal_title：Biophysical journal

authors： Morgan JE,Vakkasoglu AS,Lanyi JK,Lugtenburg J,Gennis RB,Maeda A

更新日期：2012-08-08 00:00:00

abstract：:Hint2, one of the five members of the superfamily of the histidine triad AMP-lysine hydrolase proteins, is expressed in mitochondria of various cell types. In human adrenocarcinoma cells, Hint2 modulates Ca(2+) handling by mitochondria. As Hint2 is highly expressed in hepatocytes, we investigated if this protein affec...

journal_title：Biophysical journal

authors： Ndiaye D,Collado-Hilly M,Martin J,Prigent S,Dufour JF,Combettes L,Dupont G

更新日期：2013-09-03 00:00:00

abstract：:Model compound studies in the literature show how Hofmeister ion interactions affect protein stability. Although model compound results are typically obtained as salting-out constants, they can be used to find out how the interactions affect protein stability. The null point in the Hofmeister series, which divides pro...

journal_title：Biophysical journal

authors： Baldwin RL

更新日期：1996-10-01 00:00:00

abstract：:Migratory cells are known to adapt to environments that contain wide-ranging levels of chemoattractant. Although biochemical models of adaptation have been previously proposed, here, we discuss a different mechanism based on mechanosensing, in which the interaction between biochemical signaling and cell tension facili...

journal_title：Biophysical journal

authors： Zmurchok C,Collette J,Rajagopal V,Holmes WR

更新日期：2020-10-20 00:00:00

abstract：:Sedimentation velocity analytical ultracentrifugation with fluorescence detection has emerged as a powerful method for the study of interacting systems of macromolecules. It combines picomolar sensitivity with high hydrodynamic resolution, and can be carried out with photoswitchable fluorophores for multicomponent dis...

journal_title：Biophysical journal

authors： Chaturvedi SK,Zhao H,Schuck P

更新日期：2017-04-11 00:00:00

abstract：:Electron paramagnetic resonance spectroscopy was used to monitor the orientation of muscle cross-bridges attached to actin in a low force and high stiffness state that may occur before force generation in the actomyosin cycle of interactions. 2,3-butanedione monoxime (BDM) has been shown to act as an uncompetitive inh...

journal_title：Biophysical journal

authors： Zhao L,Naber N,Cooke R

更新日期：1995-05-01 00:00:00

abstract：:Epithelial injury induces rapid recruitment of antimicrobial leukocytes to the wound site. In zebrafish larvae, activation of the epithelial NADPH oxidase Duox at the wound margin is required early during this response. Before injury, leukocytes are near the vascular region, that is, ∼100-300 μm away from the injury s...

journal_title：Biophysical journal

authors： Jelcic M,Enyedi B,Xavier JB,Niethammer P

更新日期：2017-05-09 00:00:00

abstract：:Ca2+ transport through mitochondrial Ca2+ uniporter is the primary Ca2+ uptake mechanism in respiring mitochondria. Thus, the uniporter plays a key role in regulating mitochondrial Ca2+. Despite the importance of mitochondrial Ca2+ to metabolic regulation and mitochondrial function, and to cell physiology and pathophy...

journal_title：Biophysical journal

authors： Dash RK,Qi F,Beard DA

更新日期：2009-02-18 00:00:00

abstract：:Deuterium nuclear magnetic resonance was used to monitor lipid acyl-chain orientational order in suspensions of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) containing Ca(2+) and the lung surfactant proteins SP-A and SP-B separately and together. To distinguish between protein-lipid...

journal_title：Biophysical journal

authors： Morrow MR,Temple S,Stewart J,Keough KM

更新日期：2007-07-01 00:00:00

abstract：:The structure of polymer-decorated phospholipid monolayers at the solid-solution interface was investigated using neutron reflectometry. The monolayers were composed of distearoylphosphatidylethanolamine (DSPE) matrixed with varying amounts of DSPE-PEG (DSPE with polyethylene glycol covalently grafted to its headgroup...

journal_title：Biophysical journal

authors： Kuhl TL,Majewski J,Wong JY,Steinberg S,Leckband DE,Israelachvili JN,Smith GS

更新日期：1998-11-01 00:00:00

abstract：:High-resolution measurement of membrane capacitance in the whole-cell-recording configuration can be used to detect small changes in membrane surface area that accompany exocytosis and endocytosis. We have investigated the noise of membrane capacitance measurements to determine the fundamental limits of resolution in ...

journal_title：Biophysical journal

authors： Chen P,Gillis KD

更新日期：2000-10-01 00:00:00

abstract：:Distinct lipid environments, including lipid rafts, are increasingly recognized as a crucial factor affecting membrane protein function in plasma membranes. Unfortunately, an understanding of their role in membrane protein activation and oligomerization has remained elusive due to the challenge of characterizing these...

journal_title：Biophysical journal

authors： Siegel AP,Kimble-Hill A,Garg S,Jordan R,Naumann CA

更新日期：2011-10-05 00:00:00

abstract：:Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipet...

journal_title：Biophysical journal

authors： Shevchuk A,Tokar S,Gopal S,Sanchez-Alonso JL,Tarasov AI,Vélez-Ortega AC,Chiappini C,Rorsman P,Stevens MM,Gorelik J,Frolenkov GI,Klenerman D,Korchev YE

更新日期：2016-05-24 00:00:00

abstract：:A refined mesoscopic model for the cumulative effect of repulsive excluded volume protein-protein interaction and attractive protein-surface interaction upon the properties of a trace protein capable of fiber formation is presented. The model predicts that very small changes in the magnitude of bulk volume occupancy o...

journal_title：Biophysical journal

authors： Minton AP

更新日期：2019-11-05 00:00:00