Abstract:
:Pure Michaelis-Menten enzymes have been studied (i.e., enzymes with a hyperbolic (S, V) behavior in a well-stirred solution). When such enzymes are associated with a structure in vitro, even in the simplest conceivable form (immobilization in a homogeneous gel), they can produce enzymic or transport reactions with many different kinetics (Michaelis-Menten, sigmoidal, dual-phasic, etc.). Therefore, when structured enzyme or transport processes in vivo have sigmoidal kinetics, it is not proof that the corresponding proteins are allosteric. In same manner, when the apparent kinetics are dual-phasic, it is not proof that two enzyme, or transport systems, coexist.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Vincent JC,Thellier Mdoi
10.1016/S0006-3495(83)84401-4subject
Has Abstractpub_date
1983-01-01 00:00:00pages
23-8issue
1eissn
0006-3495issn
1542-0086pii
S0006-3495(83)84401-4journal_volume
41pub_type
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