The lateral distribution of pyrene-labeled sphingomyelin and glucosylceramide in phosphatidylcholine bilayers.

abstract：:Tendons are important load-bearing structures, which are frequently injured in both sports and work. Type I collagen fibrils are the primary components of tendons and carry most of the mechanical loads experienced by the tissue, however, knowledge of how load is transmitted between and within fibrils is limited. The p...

journal_title：Biophysical journal

authors： Svensson RB,Mulder H,Kovanen V,Magnusson SP

更新日期：2013-06-04 00:00:00

abstract：:Recently a periplasmic glucose/galactose binding protein, GGRQ26C, immobilized on a gold surface has been used as an active part of a glucose biosensor based on quartz microbalance technique. However the nature of the glucose detection was not clear. Here we have found that the receptor protein film immobilized on the...

journal_title：Biophysical journal

authors： Sokolov I,Subba-Rao V,Luck LA

更新日期：2006-02-01 00:00:00

abstract：:In the intestinal epithelium, proliferated epithelial cells ascend the crypts and villi and shed at the villus tips into the gut lumen. In this study, we theoretically investigate the roles of the villi on cell turnover. We present a stochastic model that focuses on the duration over which cells migrate the shortest p...

journal_title：Biophysical journal

authors： Kai Y

更新日期：2021-01-13 00:00:00

abstract：:We present a two-component coarse-grained molecular-dynamics model for simulating the erythrocyte membrane. The proposed model possesses the key feature of combing the lipid bilayer and the erythrocyte cytoskeleton, thus showing both the fluidic behavior of the lipid bilayer and the elastic properties of the erythrocy...

journal_title：Biophysical journal

authors： Li H,Lykotrafitis G

更新日期：2012-01-04 00:00:00

abstract：:The electrostatic free energies of native and acetylated lysozymes were computed by the fixed charge model (Tanford, C., and J. G. Kirkwood, 1957, J. Am. Chem. Soc., 79:5333-5339). For each computation, the charges were transformed into a sphere of fixed radius without changing their depths or the distance between cha...

journal_title：Biophysical journal

authors： Imoto T

更新日期：1983-12-01 00:00:00

abstract：:The effect of Ca ion concentration on cross-bridge kinetics in a small bundle (one to three fibers) of chemically skinned rabbit psoas muscle is studied. The length of the muscle is oscillated in small amplitude sine waves (0.2% L0 peak-to-peak) at varying frequencies (0.125 -- 167 Hz), and the resulting amplitude and...

journal_title：Biophysical journal

authors： Kawai M,Cox RN,Brandt PW

更新日期：1981-08-01 00:00:00

abstract：:It has been a significant challenge to quantitatively study the dynamic intracellular processes in live cells. These studies are essential for a thorough understanding of the underlying mechanisms regulating the signaling pathways and the transitions between cell cycle stages. Our studies of Cdc20, an important mitoti...

journal_title：Biophysical journal

authors： Wang Z,Shah JV,Berns MW,Cleveland DW

更新日期：2006-07-01 00:00:00

abstract：:Diluted bull semen samples were bombarded with a 24 Mev proton beam. Dose response curves for the fraction of cells which survived the bombardment and for the average velocity of the surviving cells were measured. Target theory indicated a cross section of the sensitive volume of 2.1 x 10(-10) cm(2). Respiration measu...

journal_title：Biophysical journal

authors： Rikmenspoel R,van Herpen G

更新日期：1969-06-01 00:00:00

abstract：:Leukocyte capture and rolling on the vascular endothelium is mediated principally by the selectin family of cell adhesion receptors. In a parallel plate flow chamber, neutrophil rolling on purified selectins or a selectin-ligand substrate was resolved by high speed videomicroscopy as a series of ratchet-like steps wit...

journal_title：Biophysical journal

authors： Smith MJ,Berg EL,Lawrence MB

更新日期：1999-12-01 00:00:00

abstract：:We have studied the combined effects of X-irradiation and nitrogen mustard treatment on the colony-forming ability of Chinese hamster cells. In contrast to X-irradiation, nitrogen mustard acting by itself yields an exponential dose-effect relationship. Moreover, whether delivered in immediate sequence or after varying...

journal_title：Biophysical journal

authors： Sakamoto K,Elkind MM

更新日期：1969-09-01 00:00:00

abstract：:Heart surface optical mapping of transmembrane potentials has been widely used in studies of normal and pathological heart rhythms and defibrillation. In these studies, three-dimensional spatio-temporal events can only be inferred from two-dimensional surface potential maps. We present a novel optical system that enab...

journal_title：Biophysical journal

authors： Hooks DA,LeGrice IJ,Harvey JD,Smaill BH

更新日期：2001-11-01 00:00:00

abstract：:The elastic theory of flexural waves in thin rods accurately predicts the velocity of flagellar bending waves over a wide range of viscosities. This shows that flagella behave as a purely mechanical system for the transmission of these waves. An evaluation of the total bending moment reveals that this moment occurs in...

journal_title：Biophysical journal

authors： Rikmenspoel R

更新日期：1971-05-01 00:00:00

abstract：:The fundamental backbone dynamics of unfolded proteins arising due to intrinsic ϕ-ψ dihedral angle fluctuations dictate the course of protein folding, binding, assembly, and function. These internal fluctuations are also critical for protein misfolding associated with a range of human diseases. However, direct observa...

journal_title：Biophysical journal

authors： Jain N,Narang D,Bhasne K,Dalal V,Arya S,Bhattacharya M,Mukhopadhyay S

更新日期：2016-08-23 00:00:00

abstract：:The influence of the amino acid residues surrounding the flavin ring in the flavodoxin of the cyanobacterium Anabaena PCC 7119 on the electron spin density distribution of the flavin semiquinone was examined in mutants of the key residues Trp(57) and Tyr(94) at the FMN binding site. Neutral semiquinone radicals of the...

journal_title：Biophysical journal

authors： Medina M,Lostao A,Sancho J,Gómez-Moreno C,Cammack R,Alonso PJ,Martínez JI

更新日期：1999-09-01 00:00:00

abstract：:The structure and dynamics of a large segment of Ste2p, the G-protein-coupled alpha-factor receptor from yeast, were studied in dodecylphosphocholine (DPC) micelles using solution NMR spectroscopy. We investigated the 73-residue peptide EL3-TM7-CT40 consisting of the third extracellular loop 3 (EL3), the seventh trans...

journal_title：Biophysical journal

authors： Neumoin A,Arshava B,Becker J,Zerbe O,Naider F

更新日期：2007-07-15 00:00:00

abstract：:Association of transmembrane (TM) helices is facilitated by the close packing of small residues present along the amino-acid sequence. Extensive studies have established the role of such small residue motifs (GxxxG) in the dimerization of Glycophorin A (GpA) and helped to elucidate the association of TM domains in the...

journal_title：Biophysical journal

authors： Prakash A,Janosi L,Doxastakis M

更新日期：2010-12-01 00:00:00

abstract：:We have measured the static magnetization of unreduced and reduced reaction centers that vary in their quinone content. Measurements were performed in the temperature range 0.7 degrees K less than T less than 200 degrees K and magnetic fields of up to 10 kG. The electronic g-value, crystal field parameters D, E, and t...

journal_title：Biophysical journal

authors： Butler WF,Johnston DC,Shore HB,Fredkin DR,Okamura MY,Feher G

更新日期：1980-12-01 00:00:00

abstract：:We present a three-dimensional tracking routine for nondiffraction-limited particles, which significantly reduces pixel bias. Our technique allows for increased resolution compared to that of previous methods, especially at low magnification or at high signal/noise ratio. This enables tracking with nanometer accuracy ...

journal_title：Biophysical journal

authors： van Loenhout MT,Kerssemakers JW,De Vlaminck I,Dekker C

更新日期：2012-05-16 00:00:00

abstract：:Raman spectroscopic data indicate that the conformations of the two hydrocarbon chains of dipalmitoyl phosphatidylcholine in aqueous dispersions of the lipid differ signficantly. The compounds 1-palmitoyl, 2-palmitoyl-d31-3-sn-phosphatidylcholine and 1-palmitoyl-d31, 2-palmitoyl-3-sn-phosphatidylcholine were synthesiz...

journal_title：Biophysical journal

authors： Gaber BP,Yager P,Peticolas WL

更新日期：1978-12-01 00:00:00

abstract：:Cationic amino acid transporters are highly selective for L-enantiomers such as L-arginine (L-Arg). Because of this stereoselectivity, little is known about the interaction of these transporters with D-isomers. To study whether these compounds can provide information on the molecular mechanism of transport, inward cur...

journal_title：Biophysical journal

authors： Zhou J,Peluffo RD

更新日期：2010-11-17 00:00:00

abstract：:The single photon response in vertebrate phototransduction is highly reproducible despite a number of random components of the activation cascade, including the random activation site, the random walk of an activated receptor, and its quenching in a random number of steps. Here we use a previously generated and tested...

journal_title：Biophysical journal

authors： Bisegna P,Caruso G,Andreucci D,Shen L,Gurevich VV,Hamm HE,DiBenedetto E

更新日期：2008-05-01 00:00:00

abstract：:We have explored the electromechanical properties of DNA on a nanometer-length scale using an electric field to force single molecules through synthetic nanopores in ultrathin silicon nitride membranes. At low electric fields, E < 200 mV/10 nm, we observed that single-stranded DNA can permeate pores with a diameter >/...

journal_title：Biophysical journal

authors： Heng JB,Aksimentiev A,Ho C,Marks P,Grinkova YV,Sligar S,Schulten K,Timp G

更新日期：2006-02-01 00:00:00

abstract：:Artificial lipidic bilayers are widely used as a model for the lipid matrix in biological cell membranes. We use the Pockels electro-optical effect to investigate the properties of an artificial lipidic membrane doped with nonlinear molecules in the outer layer. We report here what is believed to be the first electro-...

journal_title：Biophysical journal

authors： Hajj B,De Reguardati S,Hugonin L,Le Pioufle B,Osaki T,Suzuki H,Takeuchi S,Mojzisova H,Chauvat D,Zyss J

更新日期：2009-12-02 00:00:00

abstract：:To explain the effect of profilin on actin critical concentration in a manner consistent with thermodynamic constraints and available experimental data, we built a thermodynamically rigorous model of actin steady-state dynamics in the presence of profilin. We analyzed previously published mechanisms theoretically and ...

journal_title：Biophysical journal

authors： Yarmola EG,Dranishnikov DA,Bubb MR

更新日期：2008-12-15 00:00:00

abstract：:The structure of crotapotin, a protein extracted, from the venom of the Crotalus durissus terrificus, in solution at pH = 1.5, was studied by SAXS. The experimental results yield structural parameter values of the molecular radius of gyration Rg = 13.6 A, volume v = 16.2 x 10(3) A3 A3 and maximal dimension Dmax = 46 A...

journal_title：Biophysical journal

authors： Abrego JR,Craievich AF,Mascarenhas YP,Laure CJ

更新日期：1993-02-01 00:00:00

abstract：:Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligo...

journal_title：Biophysical journal

authors： Pannwitt S,Stangl M,Schneider D

更新日期：2019-11-05 00:00:00

abstract：:We present here an efficient and accurate procedure for modeling of the three-dimensional structures of polypeptides in the explicit solvent water based on molecular dynamics calculations. Using the toxic domain analog of heat-stable enterotoxin as a model peptide, we examined the utilities of two molecular dynamics t...

journal_title：Biophysical journal

authors： Tsujishita H,Moriguchi I,Hirono S

更新日期：1994-06-01 00:00:00

abstract：:Three-dimensional structures were determined for three crystal forms of the antigen binding fragment (Fab) of anti-fluorescein antibody 4-4-20 in complex with fluorescein. These included 1) a triclinic (P1) form crystallized in 47% (v/v) 2-methyl-2,4-pentanediol (MPD); 2) a triclinic (P1) form crystallized in 16% (w/v...

journal_title：Biophysical journal

authors： Herron JN,Terry AH,Johnston S,He XM,Guddat LW,Voss EW Jr,Edmundson AB

更新日期：1994-12-01 00:00:00

abstract：:Buffered Ca(2+) diffusion in the cytosol of neuroendocrine cells is a plausible explanation for the slowness and latency in the secretion of hormones. We have developed a Monte Carlo simulation to treat the problem of 3-D diffusion and kinetic reactions of ions and buffers. The 3-D diffusion is modeled as a random wal...

journal_title：Biophysical journal

authors： Gil A,Segura J,Pertusa JA,Soria B

更新日期：2000-01-01 00:00:00

abstract：:L-type (alpha(1C)) calcium channels inactivate rapidly in response to localized elevation of intracellular Ca(2+), providing negative Ca(2+) feedback in a diverse array of biological contexts. The dominant Ca(2+) sensor for such Ca(2+)-dependent inactivation has recently been identified as calmodulin, which appears to...

journal_title：Biophysical journal

authors： Peterson BZ,Lee JS,Mulle JG,Wang Y,de Leon M,Yue DT

更新日期：2000-04-01 00:00:00