Contractile mechanisms in flagella.

abstract：:Fluorescence fluctuation spectroscopy has become an important measurement tool for investigating molecular dynamics, molecular interactions, and chemical kinetics in biological systems. Although the basic theory of fluctuation spectroscopy is well established, it is not widely recognized that saturation of the fluores...

journal_title：Biophysical journal

authors： Nagy A,Wu J,Berland KM

更新日期：2005-09-01 00:00:00

abstract：:Monolayers prepared from myelin conserve all the compositional complexity of the natural membrane when spread at the air-water interface. They show a complex pressure-dependent surface pattern that, on compression, changes from the coexistence of two liquid phases to a viscous fractal phase embedded in a liquid phase....

journal_title：Biophysical journal

authors： Rosetti CM,Maggio B

更新日期：2007-12-15 00:00:00

abstract：:The glucocorticoid receptor (GR) is a steroid-hormone-activated transcription factor that modulates gene expression. Transcriptional regulation by the GR requires dynamic receptor binding to specific target sites located across the genome. This binding remodels the chromatin structure to allow interaction with other t...

journal_title：Biophysical journal

authors： Muratcioglu S,Presman DM,Pooley JR,Grøntved L,Hager GL,Nussinov R,Keskin O,Gursoy A

更新日期：2015-09-15 00:00:00

abstract：:Cardiac contraction and relaxation dynamics result from a set of simultaneously interacting Ca(2+) regulatory mechanisms. In this study, cardiocyte Ca(2+) dynamics were modeled using a set of six differential equations that were based on theories, equations, and parameters described in previous studies. Among the uniq...

journal_title：Biophysical journal

authors： Snyder SM,Palmer BM,Moore RL

更新日期：2000-07-01 00:00:00

abstract：:Proteins that cross-link actin filaments can either form bundles of parallel filaments or isotropic networks of individual filaments. We have found that mixtures of actin filaments with alpha-actinin purified from either Acanthamoeba castellanii or chicken smooth muscle can form bundles or isotropic networks depending...

journal_title：Biophysical journal

authors： Wachsstock DH,Schwartz WH,Pollard TD

更新日期：1993-07-01 00:00:00

abstract：:In this work, we present experimental data, supported by a quantitative model, on the generation and effect of potential gradients across a tethered bilayer lipid membrane (tBLM) with, to the best of our knowledge, novel architecture. A challenge to generating potential gradients across tBLMs arises from the tethering...

journal_title：Biophysical journal

authors： Cranfield CG,Cornell BA,Grage SL,Duckworth P,Carne S,Ulrich AS,Martinac B

更新日期：2014-01-07 00:00:00

abstract：:On the basis of its role in the analysis of mammillary compartmental systems, a matrix with non-zero elements in the first row, first column, and along the main diagonal and with zero elements elsewhere is called a mammillary matrix. It is pointed out that such matrices occur in a variety of biological problems includ...

journal_title：Biophysical journal

authors： Hearon JZ

更新日期：1964-01-01 00:00:00

abstract：:A delay ( approximately 10 min) in the appearance of intracellular phage is caused by preincubating the infecting phage T4o(1) in proflavine, acridine orange, or ethidium, but not polyamines. No significant delay in attachment is observed. Apparently the presence of the dye is required inside the permeability barrier ...

journal_title：Biophysical journal

authors： Dusenbery DB,Uretz RB

更新日期：1972-08-01 00:00:00

abstract：:A phospholipid chelate complexed with ytterbium (DMPE-DTPA:Yb3+) is shown to be readily incorporated into a model membrane system, which may then be aligned in a magnetic field such that the average bilayer normal lies along the field. This so-called positively ordered smectic phase, whose lipids consist of less than ...

journal_title：Biophysical journal

authors： Prosser RS,Volkov VB,Shiyanovskaya IV

更新日期：1998-11-01 00:00:00

abstract：:Using both quasi-elastic light scattering spectroscopy and angular dissymmetry in the intensity of the scattered light, we examined the onset of turbidity for intact calf lenses and for isolated nuclear cytoplasm. In the case of the nuclear cytoplasm these measurements demonstrate the presence of two kinds of scattere...

journal_title：Biophysical journal

authors： Delaye M,Clark JI,Benedek GB

更新日期：1982-03-01 00:00:00

abstract：:The T-cell actin cytoskeleton mediates adaptive immune system responses to peptide antigens by physically directing the motion and clustering of T-cell receptors (TCRs) on the cell surface. When TCR movement is impeded by externally applied physical barriers, the actin network exhibits transient enrichment near the tr...

journal_title：Biophysical journal

authors： Smoligovets AA,Smith AW,Groves JT

更新日期：2013-08-06 00:00:00

abstract：:The interpretation of phi-values has led to an understanding of the folding transition state ensemble of a variety of proteins. Although the main guidelines and equations for calculating phi are well established, there remains some controversy about the quality of the numerical values obtained. By analyzing a complete...

journal_title：Biophysical journal

authors： Cobos ES,Candel AM,Martinez JC

更新日期：2008-06-01 00:00:00

abstract：:Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. N...

journal_title：Biophysical journal

authors： Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

更新日期：2017-09-19 00:00:00

abstract：:The space-clamped squid axon membrane and two versions of the Hodgkin-Huxley model (the original, and a strongly adapting version) are subjected to a first order dynamic analysis. Stable, repetitive firing is induced by phase-locking nerve impulses to sinusoidal currents. The entrained impulses are then pulse position...

journal_title：Biophysical journal

authors： Fohlmeister JF,Adelman WJ Jr,Poppele RE

更新日期：1980-04-01 00:00:00

abstract：:Microbial capsules are important for virulence, but their architecture and physical properties are poorly understood. The human pathogenic fungus Cryptococcus neoformans has a large polysaccharide capsule that is necessary for virulence and is the target of protective antibody responses. To study the C. neoformans cap...

journal_title：Biophysical journal

authors： Frases S,Pontes B,Nimrichter L,Rodrigues ML,Viana NB,Casadevall A

更新日期：2009-08-19 00:00:00

abstract：:Oriented solid-state NMR in combination with multiple-residue-specific (15)N labeling and extensive numerical spectral analysis is proposed to determine helix conformations of large membrane proteins in native membranes. The method is demonstrated on uniaxially oriented samples of (15)N-methionine, -valine, and -glyci...

journal_title：Biophysical journal

authors： Vosegaard T,Kamihira-Ishijima M,Watts A,Nielsen NC

更新日期：2008-01-01 00:00:00

abstract：:The effects of lipid chain packing and permeant size and shape on permeability across lipid bilayers have been investigated in gel and liquid crystalline dipalmitoylphosphatidylcholine (DPPC) bilayers by a combined NMR line-broadening/dynamic light scattering method using seven short-chain monocarboxylic acids (formic...

journal_title：Biophysical journal

authors： Xiang TX,Anderson BD

更新日期：1998-12-01 00:00:00

abstract：:The mechanism of leakage induced by surface active peptides is not yet fully understood. To gain insight into the molecular events underlying this process, the leakage induced by the peptide pardaxin from phosphatidylcholine/ phosphatidylserine/cholesterol large unilamellar vesicles was studied by monitoring the rate ...

journal_title：Biophysical journal

authors： Rapaport D,Peled R,Nir S,Shai Y

更新日期：1996-06-01 00:00:00

abstract：:DNA release from lipoplexes is an essential step during lipofection and is probably a result of charge neutralization by cellular anionic lipids. As a model system to test this possibility, fluorescence resonance energy transfer between DNA and lipid covalently labeled with Cy3 and BODIPY, respectively, was used to mo...

journal_title：Biophysical journal

authors： Tarahovsky YS,Koynova R,MacDonald RC

更新日期：2004-08-01 00:00:00

abstract：:Hybrid global optimization methods attempt to combine the beneficial features of two or more algorithms, and can be powerful methods for solving challenging nonconvex optimization problems. In this paper, novel classes of hybrid global optimization methods, termed alternating hybrids, are introduced for application as...

journal_title：Biophysical journal

authors： Klepeis JL,Pieja MJ,Floudas CA

更新日期：2003-02-01 00:00:00

abstract：:The Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzymes regulated by a voltage-sensor domain (VSD) related to the VSD found in voltage-gated ion channels. Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics compared to ion chann...

journal_title：Biophysical journal

authors： Lacroix JJ,Bezanilla F

更新日期：2012-08-08 00:00:00

abstract：:The membrane-lytic peptide melittin has previously been shown to form pores in lipid bilayers that have been described in terms of two different structural models. In the "barrel stave" model the bilayer remains more or less flat, with the peptides penetrating across the bilayer hydrocarbon region and aggregating to f...

journal_title：Biophysical journal

authors： Allende D,Simon SA,McIntosh TJ

更新日期：2005-03-01 00:00:00

abstract：:We have developed a model for the analysis of the forward creatine kinase reaction in muscle as measured by the nuclear magnetic resonance (NMR) technique of magnetization transfer. The model, accounting for the double-exponential behavior observed in some NMR magnetization transfer data, allows for the existence of t...

journal_title：Biophysical journal

authors： Zahler R,Bittl JA,Ingwall JS

更新日期：1987-06-01 00:00:00

abstract：:The effects of myosin regulatory light chain (RLC) phosphorylation and strain on adenosine diphosphate (ADP) release from cross-bridges in phasic (rabbit bladder (Rbl)) and tonic (femoral artery (Rfa)) smooth muscle were determined by monitoring fluorescence transients of the novel ADP analog, 3'-deac-eda-ADP (deac-ed...

journal_title：Biophysical journal

authors： Khromov AS,Webb MR,Ferenczi MA,Trentham DR,Somlyo AP,Somlyo AV

更新日期：2004-04-01 00:00:00

abstract：:Yeast cytosine deaminase, a zinc metalloenzyme, catalyzes the deamination of cytosine to uracil. Experimental and computational evidence indicates that the rate-limiting step is product release, instead of the chemical reaction step. In this work, we use molecular dynamics to suggest ligand exit paths. Simulation at 3...

journal_title：Biophysical journal

authors： Yao L,Yan H,Cukier RI

更新日期：2007-04-01 00:00:00

abstract：:Measurements were made of exchange rates of oxygen, carbon dioxide, and krypton-85 with blood at 37.5 degrees C. Gas transfer took place across a 1 mil silicone rubber membrane. The blood was in a rotating disk boundary layer flow, and the controlling resistance to transfer was the concentration boundary layer. Measur...

journal_title：Biophysical journal

authors： Spaeth EE,Friedlander SK

更新日期：1967-11-01 00:00:00

abstract：:We have analyzed repellent signal processing in Escherichia coli by flash photorelease of leucine from photolabile precursors. We found that 1). response amplitudes of free-swimming cell populations increased with leucine jump concentration, with an apparent Hill coefficient of 1.3 and a half-maximal dose of 14.4 micr...

journal_title：Biophysical journal

authors： Khan S,Jain S,Reid GP,Trentham DR

更新日期：2004-06-01 00:00:00

abstract：:The mechanical properties of living cells are a label-free biophysical marker of cell viability and health; however, their use has been greatly limited by low measurement throughput. Although examining individual cells at high rates is now commonplace with fluorescence activated cell sorters, development of comparable...

journal_title：Biophysical journal

authors： Sawetzki T,Eggleton CD,Desai SA,Marr DW

更新日期：2013-11-19 00:00:00

abstract：:Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V...

journal_title：Biophysical journal

authors： Umazume Y,Fujime S

更新日期：1975-02-01 00:00:00

abstract：:The S4 segments of voltage-gated sodium channels are important parts of the voltage-sensing elements of these proteins. Furthermore, the addition of the isolated S4 polypeptide to planar lipid bilayers results in stepwise increases of ion conductivity. In order to gain insight into the mechanisms of pore formation by ...

journal_title：Biophysical journal

authors： Mattila K,Kinder R,Bechinger B

更新日期：1999-10-01 00:00:00