Deuterium NMR study of the effect of ergosterol on POPE membranes.

abstract：:In high light conditions, cyanobacteria dissipate excess absorbed energy as heat in the light-harvesting phycobilisomes (PBs) to protect the photosynthetic system against photodamage. This process requires the binding of the red active form of the Orange Carotenoid Protein (OCP(r)), which can effectively quench the ex...

journal_title：Biophysical journal

authors： Tian L,Gwizdala M,van Stokkum IH,Koehorst RB,Kirilovsky D,van Amerongen H

更新日期：2012-04-04 00:00:00

abstract：:The electrical membrane potential (Vm) is one of the components of the electrochemical potential of protons across the biological membrane (proton motive force), which powers many vital cellular processes. Because Vm also plays a role in signal transduction, measuring it is of great interest. Over the years, a variety...

journal_title：Biophysical journal

authors： Mancini L,Terradot G,Tian T,Pu Y,Li Y,Lo CJ,Bai F,Pilizota T

更新日期：2020-01-07 00:00:00

abstract：:Ca2+ transport through mitochondrial Ca2+ uniporter is the primary Ca2+ uptake mechanism in respiring mitochondria. Thus, the uniporter plays a key role in regulating mitochondrial Ca2+. Despite the importance of mitochondrial Ca2+ to metabolic regulation and mitochondrial function, and to cell physiology and pathophy...

journal_title：Biophysical journal

authors： Dash RK,Qi F,Beard DA

更新日期：2009-02-18 00:00:00

abstract：:Many toxins and antimicrobial peptides permeabilize membrane vesicles by forming multimeric pores. Determination of the size of such pores is an important first step for understanding their structure and the mechanism of their self-assembly. We report a simple method for sizing pores in vesicles based on the different...

journal_title：Biophysical journal

authors： Ladokhin AS,Selsted ME,White SH

更新日期：1997-04-01 00:00:00

abstract：:The problem of the desorption of a lipid molecule from a lipid vesicle (donor) and its incorporation into another vesicle (acceptor) at high acceptor concentrations, which has been investigated experimentally (Jones, J. D. and Thompson, T. E., 1990. Biochemistry, 29:1593-1600), is analyzed here from a theoretical poin...

journal_title：Biophysical journal

authors： Almeida PF

更新日期：1999-04-01 00:00:00

abstract：:In maximally activated skinned fibers, the rate of tension redevelopment (ktr) following a rapid release and restretch is determined by the maximal rate of cross-bridge cycling. During submaximal Ca2+ activations, however, ktr regulation varies with thin filament dynamics. Thus, decreasing the rate of Ca2+ dissociatio...

journal_title：Biophysical journal

authors： Regnier M,Rivera AJ,Chase PB,Smillie LB,Sorenson MM

更新日期：1999-05-01 00:00:00

abstract：:The organization of filamentous actin and myosin II molecular motor contractility is known to modify the mechanical properties of the cell cortical actomyosin cytoskeleton. Here we describe a novel method, to our knowledge, for using force spectroscopy approach curves with tipless cantilevers to determine the actomyos...

journal_title：Biophysical journal

authors： Cartagena-Rivera AX,Logue JS,Waterman CM,Chadwick RS

更新日期：2016-06-07 00:00:00

abstract：:We argue that membrane viscosity, η(m), plays a prominent role in the thermal fluctuation dynamics of micron-scale lipid domains. A theoretical expression is presented for the timescales of domain shape relaxation, which reduces to the well-known η(m) = 0 result of Stone and McConnell in the limit of large domain size...

journal_title：Biophysical journal

authors： Camley BA,Esposito C,Baumgart T,Brown FL

更新日期：2010-09-22 00:00:00

abstract：:M2delta, one of the transmembrane segments of the nicotinic acetylcholine receptor, is a 23-amino-acid peptide, frequently used as a model for peptide-membrane interactions. In this and the companion article we describe studies of M2delta-membrane interactions, using two different computational approaches. In the pres...

journal_title：Biophysical journal

authors： Kessel A,Haliloglu T,Ben-Tal N

更新日期：2003-12-01 00:00:00

abstract：:How calmodulin (CaM) acts in KRAS-driven cancers is a vastly important question. CaM binds to and stimulates PI3Kα/Akt signaling, promoting cell growth and proliferation. Phosphorylation of CaM at Tyr99 (pY99) enhances PI3Kα activation. PI3Kα is a lipid kinase. It phosphorylates PIP2 to produce PIP3, to which Akt bind...

journal_title：Biophysical journal

authors： Zhang M,Jang H,Gaponenko V,Nussinov R

更新日期：2017-11-07 00:00:00

abstract：:Intrinsic optical signal (IOS) imaging has been widely used to map the patterns of brain activity in vivo in a label-free manner. Traditional IOS refers to changes in light transmission, absorption, reflectance, and scattering of the brain tissue. Here, we use polarized light for IOS imaging to monitor structural chan...

journal_title：Biophysical journal

authors： Koike-Tani M,Tominaga T,Oldenbourg R,Tani T

更新日期：2020-05-19 00:00:00

abstract：:In this work, we present a protocol to reconstitute membrane proteins into giant unilamellar vesicles (GUV) via peptide-induced fusion. In principle, GUV provide a well-defined lipid matrix, resembling a close-to-native state for biophysical studies, including optical microspectroscopy, of transmembrane proteins at th...

journal_title：Biophysical journal

authors： Kahya N,Pécheur EI,de Boeij WP,Wiersma DA,Hoekstra D

更新日期：2001-09-01 00:00:00

abstract：:Understanding exposure thresholds for the response of biological systems to extremely low frequency (ELF) electric and magnetic fields is a fundamental problem of long-standing interest. We consider a two-state model for voltage-gated channels in the membrane of an isolated elongated cell (Lcell = 1 mm; rcell = 25 mic...

journal_title：Biophysical journal

authors： Weaver JC,Vaughan TE,Adair RK,Astumian RD

更新日期：1998-11-01 00:00:00

abstract：:The freshwater polyp Hydra can regenerate from tissue fragments or random cell aggregates. We show that the axis-defining step ("symmetry breaking") of regeneration requires mechanical inflation-collapse oscillations of the initial cell ball. We present experimental evidence that axis definition is retarded if these o...

journal_title：Biophysical journal

authors： Soriano J,Rüdiger S,Pullarkat P,Ott A

更新日期：2009-02-18 00:00:00

abstract：:Single-molecule force spectroscopy is used to probe the kinetics of receptor-ligand bonds by applying mechanical forces to an intermediate media on which the molecules reside. When this intermediate media is a live cell, the viscoelastic properties can affect the calculation of rate constants. We theoretically investi...

journal_title：Biophysical journal

authors： Gupta VK,Neeves KB,Eggleton CD

更新日期：2012-07-03 00:00:00

abstract：:The mechanosensitive channel of small conductance (MscS) is a bacterial mechanosensitive channel that opens in response to rapid hypoosmotic stress. Since MscS can be opened solely by membrane stretch without help from any accessory protein, the lipid-protein interface must play a crucial role in sensing membrane tens...

journal_title：Biophysical journal

authors： Nomura T,Sokabe M,Yoshimura K

更新日期：2006-10-15 00:00:00

abstract：:We examined the effects of brief current pulses on the pacemaker oscillations of the Purkinje fiber using the model of McAllister , Noble, and Tsien (1975. J. Physiol. [Lond.]. 251:1-57). This model was used to construct phase-response curves for brief electric stimuli to find "black holes," where rhythmic activity of...

journal_title：Biophysical journal

authors： Chay TR,Lee YS

更新日期：1984-04-01 00:00:00

abstract：:Artificial membrane systems allow researchers to study the structure and function of membrane proteins in a matrix that approximates their natural environment and to integrate these proteins in ex vivo devices such as electronic biosensors, thin-film protein arrays, or biofuel cells. Given that most membrane proteins ...

journal_title：Biophysical journal

authors： Tunuguntla R,Bangar M,Kim K,Stroeve P,Ajo-Franklin CM,Noy A

更新日期：2013-09-17 00:00:00

abstract：:The molecular motor protein myosin VI moves toward the minus-end of actin filaments with a step size of 30-36 nm. Such large step size either drastically limits the degree of complex formation between dimer subunits to leave enough length for the lever arms, or requires an extension of the lever arms' crystallographic...

journal_title：Biophysical journal

authors： Liu Y,Hsin J,Kim H,Selvin PR,Schulten K

更新日期：2011-06-22 00:00:00

abstract：:Steady state fluorescence polarization spectra and time-resolved emission decay kinetics have been measured in vitro from malignant and normal rat kidney tissue. The degrees of polarization and emission lifetimes from the cancerous and normal systems are different. The spectroscopic differences are attributed to envir...

journal_title：Biophysical journal

authors： Tata DB,Foresti M,Cordero J,Tomashefsky P,Alfano MA,Alfano RR

更新日期：1986-09-01 00:00:00

abstract：:The engulfment function of macrophages relies on complex molecular interactions involving both lipids and proteins. In particular, the clearance of apoptotic bodies (efferocytosis) is enabled by externalization on the cell target of phosphatidylserine lipids, which activate receptors on macrophages, suggesting that (l...

journal_title：Biophysical journal

authors： Justice MJ,Petrusca DN,Rogozea AL,Williams JA,Schweitzer KS,Petrache I,Wassall SR,Petrache HI

更新日期：2014-02-04 00:00:00

abstract：:Cell/matrix adhesions are modulated by cytoskeletal or external stresses and adapt to the mechanical properties of the extracellular matrix. We propose that this mechanosensitivity arises from the activation of a mechanosensor located within the adhesion itself. We show that this mechanism accounts for the observed di...

journal_title：Biophysical journal

authors： Nicolas A,Safran SA

更新日期：2006-07-01 00:00:00

abstract：:Nuclear calcium is a key signal in the dialogue between synapse and nucleus that controls the genomic responses required for persistent adaptations, including memory and acquired neuroprotection. The amplitude and duration of nuclear calcium transients specify activity-induced transcriptional changes. However, the pre...

journal_title：Biophysical journal

authors： Bengtson CP,Freitag HE,Weislogel JM,Bading H

更新日期：2010-12-15 00:00:00

abstract：:Filopodia perform cellular functions such as environmental sensing or cell motility, but they also grab for particles and withdraw them leading to an increased efficiency of phagocytic uptake. Remarkably, withdrawal of micron-sized particles is also possible without noticeable movements of the filopodia. Here, we demo...

journal_title：Biophysical journal

authors： Kohler F,Rohrbach A

更新日期：2015-05-05 00:00:00

abstract：:Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V...

journal_title：Biophysical journal

authors： Umazume Y,Fujime S

更新日期：1975-02-01 00:00:00

abstract：:By combining the osmotic stress technique with small-angle x-ray scattering measurements, we followed the structural response of the casein micelle to an overall increase in concentration. When the aqueous phase that separates the micelles is extracted, they behave as polydisperse repelling spheres and their internal ...

journal_title：Biophysical journal

authors： Bouchoux A,Gésan-Guiziou G,Pérez J,Cabane B

更新日期：2010-12-01 00:00:00

abstract：:A new experimental approach has been developed to study the distribution of local electrostatic potential around specific protons in biologically important molecules. The approach is the development of a method denoted as "spin label/spin probe," which was proposed by one of us (. Mol. Biol. 6:498-507). The proposed m...

journal_title：Biophysical journal

authors： Likhtenshtein GI,Adin I,Novoselsky A,Shames A,Vaisbuch I,Glaser R

更新日期：1999-07-01 00:00:00

abstract：:Gigaohm recordings have been made from glutamate receptor channels in excised, outside-out patches of collagenase-treated locust muscle membrane. The channels in the excised patches exhibit the kinetic state switching first seen in megaohm recordings from intact muscle fibers. Analysis of channel dwell time distributi...

journal_title：Biophysical journal

authors： Bates SE,Sansom MS,Ball FG,Ramsey RL,Usherwood PN

更新日期：1990-07-01 00:00:00

abstract：:The theoretical relationships between the fluorescence and photochemical yields of PS II and the fraction of open reaction centers are examined in a general model endowed with the following features: i) a homogeneous, infinite PS II domain; ii) exciton-radical-pair equilibrium; and iii) different rates of exciton tran...

journal_title：Biophysical journal

authors： Lavergne J,Trissl HW

更新日期：1995-06-01 00:00:00

abstract：:The ionic strength dependence of the electron self-exchange rate constants of cytochromes c, c551, and b5 has been analyzed in terms of a monopole-dipole formalism (van Leeuwen, J.W. 1983. Biochim. Biophys. Acta. 743:408-421). The dipole moments of the reduced and oxidized forms of Ps. aeruginosa cytochrome c551 are 1...

journal_title：Biophysical journal

authors： Dixon DW,Hong X,Woehler SE

更新日期：1989-08-01 00:00:00