Abstract:
:The molecular weight of single-stranded DNA from the slime mold Physarum polycephalum has been determined by alkaline gradient centrifugation. The average molecular weight during DNA synthesis ( approximately 1.5 x 10(7) D) is less than that observed in nonsynthetic periods ( approximately 4 x 10(7) D). On the basis of a chromosome number of 50 per nucleus and a DNA content of 1 mumug per nucleus, we are led to conclude that at pH 12 each chromosome dissociates into 300 (single-stranded) pieces of DNA. We have also compared the sedimentation profiles of single-stranded DNA from Escherichia coli, PPLO, and T2 bacteriophage. These data support the conjecture that each bacterial chromosome can be dissociated into 10 or 12 single-stranded pieces of DNA. Dissociation of DNA into multiple pieces under our experimental conditions is best interpreted in terms of interruptions in the continuity of the DNA either by naturally occurring gaps or at alkali-labile bonds.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
McGrath RA,Williams RWdoi
10.1016/S0006-3495(67)86590-1subject
Has Abstractpub_date
1967-05-01 00:00:00pages
309-17issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(67)86590-1journal_volume
7pub_type
杂志文章abstract::Entropy Sampling Monte Carlo (ESMC) simulations were carried out to study the thermodynamics of the folding transition in the GCN4 leucine zipper (GCN4-lz) in the context of a reduced model. Using the calculated partition functions for the monomer and dimer, and taking into account the equilibrium between the monomer ...
journal_title:Biophysical journal
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journal_title:Biophysical journal
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更新日期:1998-01-01 00:00:00
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更新日期:1964-05-01 00:00:00
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journal_title:Biophysical journal
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更新日期:2009-03-18 00:00:00
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journal_title:Biophysical journal
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更新日期:1997-11-01 00:00:00
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更新日期:1986-10-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2007-03-15 00:00:00
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更新日期:2006-07-15 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2016-08-23 00:00:00
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更新日期:1989-11-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2015-10-20 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1988-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.bpj.2010.08.023
更新日期:2010-10-20 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.040014
更新日期:2004-07-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2017-02-28 00:00:00
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更新日期:1992-03-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.055517
更新日期:2005-03-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
abstract::The electrogenic transport of ATP and ADP by the mitochondrial ADP/ATP carrier (AAC) was investigated by recording transient currents with two different techniques for performing concentration jump experiments: 1) the fast fluid injection method: AAC-containing proteoliposomes were adsorbed to a solid supported membra...
journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1999-08-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1987-03-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1996-08-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
abstract::Detection of the transition between the two myosin isoforms α- and β-myosin in living cardiomyocytes is essential for understanding cardiac physiology and pathology. In this study, the differences in symmetry of polarization spectra obtained from α- and β-myosin in various mammalian ventricles and propylthiouracil-tre...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2019.12.030
更新日期:2020-03-10 00:00:00
abstract::The mechanosensitive channel of small conductance (MscS) is a bacterial mechanosensitive channel that opens in response to rapid hypoosmotic stress. Since MscS can be opened solely by membrane stretch without help from any accessory protein, the lipid-protein interface must play a crucial role in sensing membrane tens...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.084541
更新日期:2006-10-15 00:00:00