Mechanism of DNA compaction by yeast mitochondrial protein Abf2p.

abstract：:The sequence of events involved in the transition from attached liposomes to bilayer patches on hydrophilic and hydrophobic solid supports were visualized in situ by Tapping Mode atomic force microscopy in liquid. In a smooth manner, the attached liposomes spread and flattened from the outer edges toward the center un...

journal_title：Biophysical journal

authors： Jass J,Tjärnhage T,Puu G

更新日期：2000-12-01 00:00:00

abstract：:We have interrogated the isothermal folding behavior of the DNA i-motif of the human telomere, dC19, and a high-stability i-motif-forming sequence in the promoter of the human DNA repair gene RAD17 using human physiological solution and temperature conditions. We developed a circular-dichroism-spectroscopy-based pH ti...

journal_title：Biophysical journal

authors： Rogers RA,Fleming AM,Burrows CJ

更新日期：2018-04-24 00:00:00

abstract：:The reaction of c-cytochromes with iron hexacyanides is similar in mechanism to the interaction of cytochromes with their physiological oxidants and reductants in that the formation of complexes precedes electron transfer. Analysis of the kinetics of oxidation and reduction of a number of c-cytochromes by solving the ...

journal_title：Biophysical journal

authors： Ohno N,Cusanovich MA

更新日期：1981-12-01 00:00:00

abstract：:A beat-to-beat variation in the cardiac action potential duration is a phenomenon known as alternans. Alternans has been linked to ventricular fibrillation, and thus the ability to predict the onset of alternans could be clinically beneficial. Theoretically, it has been proposed that the slope of a restitution curve, ...

journal_title：Biophysical journal

authors： Cram AR,Rao HM,Tolkacheva EG

更新日期：2011-02-16 00:00:00

abstract：:We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich ...

journal_title：Biophysical journal

authors： Owen DM,Lanigan PM,Dunsby C,Munro I,Grant D,Neil MA,French PM,Magee AI

更新日期：2006-06-01 00:00:00

abstract：:A detailed picture of water and ion properties in small pores is important for understanding the behavior of biological ion channels. Several recent modeling studies have shown that small, hydrophobic pores exclude water and ions even if they are physically large enough to accommodate them, a mechanism called hydropho...

journal_title：Biophysical journal

authors： Spronk SA,Elmore DE,Dougherty DA

更新日期：2006-05-15 00:00:00

abstract：:In cardiac muscle, troponin (Tn) and tropomyosin inhibit actin and myosin interactions through the steric blocking of myosin binding to F-actin. Ca2+ binding to Tn C modulates this inhibition. Thin filaments become activated upon Ca2+ binding, which enables strong binding of myosin with a concomitant release of ATP hy...

journal_title：Biophysical journal

authors： Solís C,Kim GH,Moutsoglou ME,Robinson JM

更新日期：2018-11-06 00:00:00

abstract：:Interactions between amyloid-β peptide (Aβ) and the cell membrane include interaction with membrane lipids and binding to membrane receptors, both of which are considered to be the toxicity mechanisms of Aβ. However, it is unclear whether both mechanisms lead to cytotoxicity. Thus, we aimed to analyze these two mechan...

journal_title：Biophysical journal

authors： Shi JM,Zhu L,Lan X,Zhao DW,He YJ,Sun ZQ,Wu D,Li HY

更新日期：2020-09-15 00:00:00

abstract：:Two piezoelectric constants (polarization per unit stress, d=d'-id'', and polarization per unit strain, e=e'-ie''), the elastic constant, and dielectric constant are determined for oriented collagen at different hydration levels at 10 Hz from -150 to 50 degrees C. With no hydration (approximately 0% H2O), d' increases...

journal_title：Biophysical journal

authors： Fukada E,Ueda H,Rinaldi R

更新日期：1976-08-01 00:00:00

abstract：:Regulation of the F-actin severing activity of gelsolin by Ca2+ has been investigated under physiologic ionic conditions. Tryptophan fluorescence intensity measurements indicate that gelsolin contains at least two Ca2+ binding sites with affinities of 2.5 x 10(7) M-1 and 1.5 x 10(5) M-1. At F-actin and gelsolin concen...

journal_title：Biophysical journal

authors： Kinosian HJ,Newman J,Lincoln B,Selden LA,Gershman LC,Estes JE

更新日期：1998-12-01 00:00:00

abstract：:Cofilin/ADF, beryllium fluoride complex (BeFx), and phalloidin have opposing effects on actin filament structure and dynamics. Cofilin/ADF decreases the stability of F-actin by enhancing disorder in subdomain 2, and by severing and accelerating the depolymerization of the filament. BeFx and phalloidin stabilize the su...

journal_title：Biophysical journal

authors： Muhlrad A,Ringel I,Pavlov D,Peyser YM,Reisler E

更新日期：2006-12-15 00:00:00

abstract：:The oligomeric structure was determined for four recombinant kinesin derivatives containing N-terminal fragments of the kinesin alpha-subunit. Some of the proteins were dimeric (two-headed) molecules with mechanochemical properties similar to those of intact kinesin. Comparison of the primary and quaternary structures...

journal_title：Biophysical journal

authors： Gelles J,Berliner E,Young EC,Mahtani HK,Perez-Ramirez B,Anderson K

更新日期：1995-04-01 00:00:00

abstract：:Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although the crystal structures of dark- and light-adapted states have been determined, the molecular mechanisms underlying photoactivation remain elusive. Here, we demonstrate that the conserved tongue region of...

journal_title：Biophysical journal

authors： Gustavsson E,Isaksson L,Persson C,Mayzel M,Brath U,Vrhovac L,Ihalainen JA,Karlsson BG,Orekhov V,Westenhoff S

更新日期：2020-01-21 00:00:00

abstract：:The organization of the normal collagen molecule and fibrils is reviewed and the detection, assay, and isolation of a collagenolytic enzyme from amphibian tadpole tissue are described and its possible significance in metamorphosis is discussed ...

journal_title：Biophysical journal

authors： GROSS J

更新日期：1964-01-01 00:00:00

abstract：:We describe Franck-Condon simulations of vibrational cooling effects on absorption difference spectra in chlorophyll a (Chl a). The relative contributions of vibrational equilibration in the electronic ground and excited states depend on the pump and probe wavelengths. For Franck-Condon-active vibrational modes exhibi...

journal_title：Biophysical journal

authors： Struve WS

更新日期：1995-12-01 00:00:00

abstract：:Kv1 potassium channels are widely distributed in mammalian tissues and are involved in a variety of functions from controlling the firing rate of neurons to maturation of T-lymphocytes. Here we show that the newly described KCNE4 beta-subunit has a drastic inhibitory effect on currents generated by Kv1.1 and Kv1.3 pot...

journal_title：Biophysical journal

authors： Grunnet M,Rasmussen HB,Hay-Schmidt A,Rosenstierne M,Klaerke DA,Olesen SP,Jespersen T

更新日期：2003-09-01 00:00:00

abstract：:Electron-electron double resonance (ELDOR) and saturation-recovery spectroscopy employing 14N:15N stearic acid spin-label pairs have been used to study the effects of cholesterol on lateral diffusion and vertical fluctuations in lipid bilayers. The 14N:15N continuous wave electron-electron double resonance (CW ELDOR) ...

journal_title：Biophysical journal

authors： Yin JJ,Feix JB,Hyde JS

更新日期：1987-12-01 00:00:00

abstract：:The processes of cell growth and budding of the yeast cells Saccharomyces cerevisiae, which were gently immobilized on 3% agar and submerged in culture medium, were successfully imaged with an atomic force microscope for 6-7 h. Similar experiments on chemically fixed cells did not detect any appreciable change in thei...

journal_title：Biophysical journal

authors： Gad M,Ikai A

更新日期：1995-12-01 00:00:00

abstract：:A newly developed microscope using acousto-optic tunable filters (AOTFs) was used to generate in vivo hemoglobin saturation (SO2) and oxygen tension (PO2) maps in the cerebral cortex of mice. SO2 maps were generated from the spectral analysis of reflected absorbance images collected at different wavelengths, and PO2 m...

journal_title：Biophysical journal

authors： Shonat RD,Wachman ES,Niu W,Koretsky AP,Farkas DL

更新日期：1997-09-01 00:00:00

abstract：:Cellular processes are noisy due to the stochastic nature of biochemical reactions. As such, it is impossible to predict the exact quantity of a molecule or other attributes at the single-cell level. However, the distribution of a molecule over a population is often deterministic and is governed by the underlying regu...

journal_title：Biophysical journal

authors： Li B,You L

更新日期：2014-09-02 00:00:00

abstract：:An analytical electromechanical model of a spherical cell exposed to an alternating electric field was used to calculate shear stress generated in the cellular membrane. Shape deformation of Neurospora crassa (slime) spheroplasts was measured. Statistical analysis permitted empirical evaluation of creep of the cellula...

journal_title：Biophysical journal

authors： Poznański J,Pawłowski P,Fikus M

更新日期：1992-03-01 00:00:00

abstract：:Spontaneous conversion of beta-amyloid peptide (Abeta) from soluble monomer to insoluble fibril may underlie the neurodegeneration associated with Alzheimer's disease. A complete description of Abeta self-association kinetics requires identification of the oligomeric species present and the pathway of association, as ...

journal_title：Biophysical journal

authors： Pallitto MM,Murphy RM

更新日期：2001-09-01 00:00:00

abstract：:Some new approaches to the kinetic study of the reduction of cytochrome aa3 by cytochrome c are presented. The primary innovations are the use of a spectrometer which can acquire multiwavelength data as fast as every 10 microseconds, and the application of a variety of analytical methods which can utilize simultaneous...

journal_title：Biophysical journal

authors： Hendler RW,Bose SK,Shrager RI

更新日期：1993-09-01 00:00:00

abstract：:We developed a multicellular model characterized by a high degree of heterogeneity to investigate possible mechanisms that underlie circadian network synchronization and rhythmicity in the suprachiasmatic nucleus (SCN). We populated a two-dimensional grid with 400 model neurons coupled via γ-aminobutyric acid (GABA) a...

journal_title：Biophysical journal

authors： Vasalou C,Herzog ED,Henson MA

更新日期：2011-07-06 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) has been used to measure the diffusion of fluorescently labeled beads in solutions of polymerized actin or buffer. The results, obtained at actin concentrations of 1 mg/ml, show that small beads (0.09 micron in diameter) diffuse nearly as rapidly in the actin gel as in buffe...

journal_title：Biophysical journal

authors： Qian H,Elson EL,Frieden C

更新日期：1992-10-01 00:00:00

abstract：:The opening of basepairs plays a key role in DNA replication and transcription, and in the action of DNA repair and modification enzymes. In this article, we have used proton exchange to define the energetics of the pathways for basepair opening in two DNA 17-mer duplexes. The rates of exchange of imino protons with s...

journal_title：Biophysical journal

authors： Coman D,Russu IM

更新日期：2005-11-01 00:00:00

abstract：:Wiener kernels have been measured for the light growth response of a number of mutants of Phycomyces which show abnormal phototropism (mad mutants). Representative mutants were chosen from the six complementation groups (madA to madF) associated with the light response pathway. One group, madA, associated with the inp...

journal_title：Biophysical journal

authors： Lipson ED

更新日期：1975-10-01 00:00:00

abstract：:Crystals of ion-free gramicidin A (P212121: a = 24.61, b = 32.28, c = 32.52) have been investigated using neutron diffraction. A difference analysis of crystals soaked in ethanol/H2O as opposed to ethanol-d6/D2O has led to single isomorphous replacement Fourier projections of the structure at 5-A resolution. The grami...

journal_title：Biophysical journal

authors： Koeppe RE 2nd,Schoenborn BP

更新日期：1984-03-01 00:00:00

abstract：:We study the effect of ergosterol on the physical properties of 1-[(2)H(31)]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance. NMR spectra were taken as a function of temperature and ergosterol concentration up to 70 mol %. The spectral first moments sh...

journal_title：Biophysical journal

authors： Hsueh YW,Weng CJ,Chen MT,Thewalt J,Zuckermann M

更新日期：2010-04-07 00:00:00

abstract：:The ability to discriminate between different cations efficiently is essential for the proper physiological functioning of many membrane transport proteins. One obvious mechanism of ion selectivity is when a binding site is structurally constrained by the protein architecture and its geometry is precisely adapted to f...

journal_title：Biophysical journal

authors： Roux B

更新日期：2010-06-16 00:00:00