Abstract:
:We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Owen DM,Lanigan PM,Dunsby C,Munro I,Grant D,Neil MA,French PM,Magee AIdoi
10.1529/biophysj.106.084673subject
Has Abstractpub_date
2006-06-01 00:00:00pages
L80-2issue
11eissn
0006-3495issn
1542-0086pii
S0006-3495(06)72563-2journal_volume
90pub_type
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