Abstract:
:The spatiotemporal organization and dynamics of the plasma membrane and its constituents are central to cellular function. Fluorescence-based single-particle tracking has emerged as a powerful approach for studying the single molecule behavior of plasma-membrane-associated events because of its excellent background suppression, at the expense of imaging speed and observation time. Here, we show that interferometric scattering microscopy combined with 40 nm gold nanoparticle labeling can be used to follow the motion of membrane proteins in the plasma membrane of live cultured mammalian cell lines and hippocampal neurons with up to 3 nm precision and 25 μs temporal resolution. The achievable spatiotemporal precision enabled us to reveal signatures of compartmentalization in neurons likely caused by the actin cytoskeleton.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
de Wit G,Albrecht D,Ewers H,Kukura Pdoi
10.1016/j.bpj.2018.05.007subject
Has Abstractpub_date
2018-06-19 00:00:00pages
2945-2950issue
12eissn
0006-3495issn
1542-0086pii
S0006-3495(18)30582-4journal_volume
114pub_type
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