Abstract:
:The patch-clamp technique of Mueller (1975, Ann. N.Y. Acad. Sci., 274:247-264) and Neher and Sakmann (1976, Nature (Lond.), 260:799-802) was modified to be suitable for single-channel measurements in lipid bilayers at potentials up to 500 mV. This method was used to study gramicidin A single-channel current-voltage characteristics. It was found that the sublinear current-voltage behavior normally observed at low permeant ion concentrations and rather low potentials (V less than or equal to 200 mV) continues to be seen all the way up to 500 mV. This phenomenon is characteristic of the low permeant ion situation in which the channel is far from saturation, and implies that the overall rate constant for association between ion and channel is very weakly, if at all, voltage dependent. The magnitude of the single channel currents at 500 mV is consistent with the notion that the aqueous convergence conductance is a significant factor in determining the permeability characteristics of the gramicidin A channel.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Andersen OSdoi
10.1016/S0006-3495(83)84414-2subject
Has Abstractpub_date
1983-02-01 00:00:00pages
119-33issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(83)84414-2journal_volume
41pub_type
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journal_title:Biophysical journal
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更新日期:2004-04-01 00:00:00
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pub_type: 杂志文章
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