The kinetics of the hydrogen/deuterium exchange of epidermal growth factor receptor ligands.

abstract：:Tendons are important load-bearing structures, which are frequently injured in both sports and work. Type I collagen fibrils are the primary components of tendons and carry most of the mechanical loads experienced by the tissue, however, knowledge of how load is transmitted between and within fibrils is limited. The p...

journal_title：Biophysical journal

authors： Svensson RB,Mulder H,Kovanen V,Magnusson SP

更新日期：2013-06-04 00:00:00

abstract：:Platinum-containing molecules are widely used as anticancer drugs. These molecules exert cytotoxic effects by binding to DNA through various mechanisms. The binding between DNA and platinum-based drugs hinders the opening of DNA, and therefore, DNA duplication and transcription are severely hampered. Overall, impeding...

journal_title：Biophysical journal

authors： Salerno D,Beretta GL,Zanchetta G,Brioschi S,Cristofalo M,Missana N,Nardo L,Cassina V,Tempestini A,Giovannoni R,Cerrito MG,Zaffaroni N,Bellini T,Mantegazza F

更新日期：2016-05-24 00:00:00

abstract：:Intracellular Ca2+ was determined with the fura-2 technique during electrically induced contractions in the rabbit rectococcygeus smooth muscle at 22 degreesC. The muscles were electrically activated to give short, reproducible contractions. Intracellular [Ca2+] increased during activation; the increase in [Ca2+] prec...

journal_title：Biophysical journal

authors： Arner A,Malmqvist U,Rigler R

更新日期：1998-10-01 00:00:00

abstract：:The nuclear magnetic resonance (NMR) of water protons in live and glycerinated muscle, suspensions of glycerinated myofibrils, and solutions of several muscle proteins has been studied. T(1) and T(2), measured on partially hydrated proteins by pulsed spin-echo techniques, decreased as the ratio of water to protein dec...

journal_title：Biophysical journal

authors： Cooke R,Wien R

更新日期：1971-12-01 00:00:00

abstract：:The nuclei of epididymal sperm, isolated from C57BL/6J and CBA/J inbred mice by their resistance to trypsin digestion, retain the shape differences of the intact sperm head. Various physical characteristics of these nuclei were measured and compared. The measurement of the projected dimensions of nuclei showed that th...

journal_title：Biophysical journal

authors： Wyrobek AJ,Meistrich ML,Furrer R,Bruce WR

更新日期：1976-07-01 00:00:00

abstract：:The separation method, flow field-flow fractionation (flow FFF), is coupled on-line with multiangle laser light scattering (MALLS) for simultaneous measurement of the size and concentration of vesicles eluting continuously from the fractionator. These size and concentration data, gathered as a function of elution time...

journal_title：Biophysical journal

authors： Korgel BA,van Zanten JH,Monbouquette HG

更新日期：1998-06-01 00:00:00

abstract：:The scattering and absorption coefficients of many homogeneous biological tissues such as muscle, skin, white matter in the brain, and dentin are often anisotropically oriented with respect to their bounding interface. In consequence the curves of equal intensity of re-emitted light on the surface of the slab will no ...

journal_title：Biophysical journal

authors： Dudko OK,Weiss GH

更新日期：2005-05-01 00:00:00

abstract：:A computational framework is presented for studying the mechanical response of macromolecules. The method combines a continuum mechanics (CM) model for the mechanical properties of the macromolecule with a continuum electrostatic (CE) treatment of solvation. The molecules are represented by their shape and key physico...

journal_title：Biophysical journal

authors： Ma L,Yethiraj A,Chen X,Cui Q

更新日期：2009-05-06 00:00:00

abstract：:Small, icosahedral double-stranded DNA bacteriophage pack their genomes tightly into preformed protein capsids using an ATP-driven motor. Coarse-grain molecular-mechanics models provide a detailed picture of DNA packaging in bacteriophage, revealing how conformation depends on capsid size and shape, and the presence o...

journal_title：Biophysical journal

authors： Petrov AS,Harvey SC

更新日期：2008-07-01 00:00:00

abstract：:We have determined directly the effects of the inhibitory peptide phospholamban (PLB) on the rotational dynamics of the calcium pump (Ca-ATPase) of cardiac sarcoplasmic reticulum (SR). This was accomplished by comparing mouse ventricular SR, which has PLB levels similar to those found in other mammals, with mouse atri...

journal_title：Biophysical journal

authors： Voss JC,Mahaney JE,Jones LR,Thomas DD

更新日期：1995-05-01 00:00:00

abstract：:Small single-domain proteins often exhibit only a single free-energy barrier, or transition state, between the denatured and the native state. The folding kinetics of these proteins is usually explored via mutational analysis. A central question is which structural information on the transition state can be derived fr...

journal_title：Biophysical journal

authors： Weikl TR

更新日期：2008-02-01 00:00:00

abstract：:The self-assembly of myosin into filamentous structures is a highly cooperative and rapid process. Nevertheless, the presence of nonequivalent bonding interactions within the filament permits differential stabilization of several macromolecular assemblies of myosin under well-controlled ionic conditions in citrate/Tri...

journal_title：Biophysical journal

authors： Reisler E,Cheung P,Borochov N

更新日期：1986-01-01 00:00:00

abstract：:Nuclear transport receptors (NTRs) mediate nucleocytoplasmic transport via their affinity for unstructured proteins (polymers) in the nuclear pore complex (NPC). Here, we have modeled the effect of NTRs on polymeric structure in the nanopore confinement of the NPC central conduit. The model explicitly takes into accou...

journal_title：Biophysical journal

authors： Osmanović D,Ford IJ,Hoogenboom BW

更新日期：2013-12-17 00:00:00

abstract：:The time-resolved thermodynamics of the flavin mononucleotide (FMN)-binding LOV1 domain of Chlamydomonas reinhardtii phot (phototropin homolog) was studied by means of laser-induced optoacoustic spectroscopy. In the wild-type protein the early red-shifted intermediate LOV(715) exhibits a small volume contraction, Delt...

journal_title：Biophysical journal

authors： Losi A,Kottke T,Hegemann P

更新日期：2004-02-01 00:00:00

abstract：:The energetics of phospholipid aggregation depend on the apparent water-accessible apolar surface area (ASAap), ordering effects of the chains, and headgroup interactions. We quantify the enthalpy and entropy of these interactions separately. For that purpose, the thermodynamics of micelle formation of lysophosphatidy...

journal_title：Biophysical journal

authors： Heerklotz H,Epand RM

更新日期：2001-01-01 00:00:00

abstract：:In the intestinal epithelium, proliferated epithelial cells ascend the crypts and villi and shed at the villus tips into the gut lumen. In this study, we theoretically investigate the roles of the villi on cell turnover. We present a stochastic model that focuses on the duration over which cells migrate the shortest p...

journal_title：Biophysical journal

authors： Kai Y

更新日期：2021-01-13 00:00:00

abstract：:A novel combined approach of molecular dynamics (MD) and Monte Carlo simulations is developed to calculate various free-volume distributions as a function of position in a lipid bilayer membrane at 323 K. The model bilayer consists of 2 x 100 chain molecules with each chain molecule having 15 carbon segments and one h...

journal_title：Biophysical journal

authors： Xiang TX

更新日期：1993-09-01 00:00:00

abstract：:Proteins fold and function inside cells that are crowded with macromolecules. Here, we address the role of the resulting excluded volume effects by in vitro spectroscopic studies of Pseudomonas aeruginosa apoazurin stability (thermal and chemical perturbations) and folding kinetics (chemical perturbation) as a functio...

journal_title：Biophysical journal

authors： Christiansen A,Wittung-Stafshede P

更新日期：2013-10-01 00:00:00

abstract：:The lateral mobility of the lipid analog N-4-nitrobenzo-2-oxa-1,3 diazole phosphatidylethanolamine and of the integral protein glycophorin in giant dimyristoylphosphatidylcholine vesicles was studied by the photobleaching technique. Above the temperature of the chain-melting transition (Tm = 23 degrees C), the diffusi...

journal_title：Biophysical journal

authors： Kapitza HG,Rüppel DA,Galla HJ,Sackmann E

更新日期：1984-03-01 00:00:00

abstract：:The antifungal polyene antibiotic amphotericin B forms channels in lipid membranes that are permeable to ions, water, and nonelectrolytes. Anion, cation, and ion pair coordination in the water-filled pore of the "barrel" unit of the channels was studied by molecular dynamics simulations. Unlike the case of the gramici...

journal_title：Biophysical journal

authors： Khutorsky V

更新日期：1996-12-01 00:00:00

abstract：:A newly developed water-soluble phosphor suitable for measuring oxygen pressure in the blood (Green 2W) was used for noninvasive, in vivo imaging of oxygen distribution in the vascular systems of mice. Oxygen quenches the phosphorescence of Green 2W, measured in the presence of 2% albumin, according to the Stern-volme...

journal_title：Biophysical journal

authors： Vinogradov SA,Lo LW,Jenkins WT,Evans SM,Koch C,Wilson DF

更新日期：1996-04-01 00:00:00

abstract：:Stationary and kinetic experiments were performed on lipid bilayer membranes to study the mechanism of iodine- and bromine-mediated halide transport in detail. The stationary conductance data suggested that four different 1:1 complexes between I2 and Br2 and the halides I- and Br- were responsible for the observed con...

journal_title：Biophysical journal

authors： Klotz KH,Benz R

更新日期：1993-12-01 00:00:00

abstract：:Current viral docking models have relied upon the assumption that bond formation and breakage are independent of viral and docking surface geometry, as well as the forces exerted on the bonds. This assumption, known as the equivalent site hypothesis (ESH), is examined in detail using a newly developed simulation techn...

journal_title：Biophysical journal

authors： English TJ,Hammer DA

更新日期：2004-06-01 00:00:00

abstract：:Atomistic molecular dynamics simulations are used to probe changes in the nature and subnanosecond dynamical behavior of solvation waters that accompany partial denaturation of the globular protein, human alpha-lactalbumin. A simulated ensemble of subcompact conformers, similar to the molten globule state of human alp...

journal_title：Biophysical journal

authors： Sengupta N,Jaud S,Tobias DJ

更新日期：2008-12-01 00:00:00

abstract：:The wormlike-chain (WLC) model is widely used to describe the energetics of DNA bending. Motivated by recent experiments, alternative, so-called subelastic chain models were proposed that predict a lower elastic energy of highly bent DNA conformations. Until now, no unambiguous verification of these models has been ob...

journal_title：Biophysical journal

authors： Schöpflin R,Brutzer H,Müller O,Seidel R,Wedemann G

更新日期：2012-07-18 00:00:00

abstract：:Recent advances in imaging technology have revealed oscillations of cyclic adenosine monophosphate (cAMP) in insulin-secreting cells. These oscillations may be in phase with cytosolic calcium oscillations or out of phase. cAMP oscillations have previously been modeled as driven by oscillations in calcium, based on the...

journal_title：Biophysical journal

authors： Peercy BE,Sherman AS,Bertram R

更新日期：2015-07-21 00:00:00

abstract：:A new solid-state NMR-based strategy is established for the precise and efficient analysis of orientation and dynamics of transmembrane peptides in fluid bilayers. For this purpose, several dynamically averaged anisotropic constraints, including (13)C and (15)N chemical shift anisotropies and (13)C-(15)N dipolar coupl...

journal_title：Biophysical journal

authors： Holt A,Rougier L,Réat V,Jolibois F,Saurel O,Czaplicki J,Killian JA,Milon A

更新日期：2010-05-19 00:00:00

abstract：:Object contrast is one of the most important parameters of macromolecular imaging. Low-voltage transmission electron microscopy has shown an increased atom contrast for carbon materials, indicating that amplitude contrast contributions increase at a higher rate than phase contrast and inelastic scattering. Here, we st...

journal_title：Biophysical journal

authors： Majorovits E,Angert I,Kaiser U,Schröder RR

更新日期：2016-02-23 00:00:00

abstract：:Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. N...

journal_title：Biophysical journal

authors： Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

更新日期：2017-09-19 00:00:00

abstract：:Orientational order parameters of two diphenylhexatriene (DPH)-based fluorescent probes, 2-(3-(diphenylhexatrienyl)propanoyl)-1-hexadecanoyl-sn-glycero-3-p hosphocholine (DPHpPC) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH), in dipalmitoylphosphatidylcholine (DPPC) Langmuir-Blodgett monolayers...

journal_title：Biophysical journal

authors： Zhai X,Kleijn JM

更新日期：1997-06-01 00:00:00