Na(+) transport, and the E(1)P-E(2)P conformational transition of the Na(+)/K(+)-ATPase.

abstract：:Small-angle neutron and x-ray scattering experiments have been performed on type 2 tissular transglutaminase to characterize the conformational changes that bring about Ca(2+) activation and guanosine triphosphate (GTP) inhibition. The native and a proteolyzed form of the enzyme, in the presence and in the absence of ...

journal_title：Biophysical journal

authors： Mariani P,Carsughi F,Spinozzi F,Romanzetti S,Meier G,Casadio R,Bergamini CM

更新日期：2000-06-01 00:00:00

abstract：:Numerous isotypes of the structural protein tubulin have now been characterized in various organisms and their expression offers a plausible explanation for observed differences affecting microtubule function in vivo. While this is an attractive hypothesis, there are only a handful of studies demonstrating a direct in...

journal_title：Biophysical journal

authors： Rezania V,Azarenko O,Jordan MA,Bolterauer H,Ludueña RF,Huzil JT,Tuszynski JA

更新日期：2008-08-01 00:00:00

abstract：:Multiphasic kinetics are often observed in stopped-flow investigations. To characterize further these kinetic phases, we have developed a methodology whereby fluorescence total intensity and anisotropy stopped-flow data can be combined in a single analysis. Fluorescence total intensity and anisotropy are highly interr...

journal_title：Biophysical journal

authors： Otto MR,Lillo MP,Beechem JM

更新日期：1994-12-01 00:00:00

abstract：:A requirement for concentrated and chemically homogeneous pools of molecular building blocks would severely restrict plausible scenarios for the origin of life. In the case of membrane self-assembly, models of prebiotic lipid synthesis yield primarily short, single-chain amphiphiles that can form bilayer vesicles only...

journal_title：Biophysical journal

authors： Budin I,Prwyes N,Zhang N,Szostak JW

更新日期：2014-10-07 00:00:00

abstract：:Thin filament regulation of contraction is thought to involve the binding of two activating ligands: Ca2+ and strongly bound cross-bridges. The specific cross-bridge states required to promote thin filament activation have not been identified. This study examines the relationship between cross-bridge cycling and thin ...

journal_title：Biophysical journal

authors： Wahr PA,Metzger JM

更新日期：1999-04-01 00:00:00

abstract：:Knowledge about the influenza fusion peptide (FP) membrane insertion mode is crucial for understanding its fusogenic mechanism. NMR and electron paramagnetic resonance experiments showed that in micelles, the FP inserted as a fixed-angle inverted V. In membranes, however, it was shown to insert as a straight α-helix (...

journal_title：Biophysical journal

authors： Légaré S,Lagüe P

更新日期：2012-05-16 00:00:00

abstract：:Formation of amyloid oligomers, the most toxic species of amyloids in degenerative diseases, is critically coupled to the interplay with surrounding water. The hydrophobic force driving the oligomerization causes water removal from interfaces, changing the surface-hydration properties. Here, we show that such effects ...

journal_title：Biophysical journal

authors： Walton JH,Berry RS,Despa F

更新日期：2011-05-04 00:00:00

abstract：:Purified cytochrome c oxidase CO complex from beef heart has been studied by Fourier transform infrared absorbance difference spectroscopy. Photolysis at 10-20 Kelvin results in dissociation of a3FeCO, formation of CuBCO, and perturbation of the a3-heme and CuB complex. The vibrational perturbation spectrum between 90...

journal_title：Biophysical journal

authors： Park S,Pan LP,Chan SI,Alben JO

更新日期：1996-08-01 00:00:00

abstract：:Some membrane peptides, such as Alamethicin, form barrel-stave aggregates with a broad probability distribution of size (number of peptides in the aggregate). This distribution has been shown to depend on the characteristics of the lipid bilayer. A mechanism for this influence is suggested, in analogy to earlier work ...

journal_title：Biophysical journal

authors： Cantor RS

更新日期：2002-05-01 00:00:00

abstract：:In this paper, we consider the effect of adding small carbohydrate solutes (small sugars) to DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) L(alpha) dispersions and the consequences on the force balance at zero osmotic pressure (maximal swelling). We show the importance of long incubations required to obtain sampl...

journal_title：Biophysical journal

authors： Demé B,Dubois M,Zemb T

更新日期：2002-01-01 00:00:00

abstract：:Voltage-sensing dyes and voltage-sensing fluorescence proteins have been continually improved and as a result have provided a wealth of insights into neuronal circuits. Further improvements in voltage-sensing dyes and voltage-sensing fluorescence proteins are needed, however, for routine detection of single action pot...

journal_title：Biophysical journal

authors： Park K,Weiss S

更新日期：2017-02-28 00:00:00

abstract：:The lateral distribution of N-[10(1-pyrenyl)decanoyl]-sphingomyelin (PyrSPM) and N-[10(1-pyrenyl)decanoyl]-glucocerebroside (PyrGlcCer) was studied in multilamellar vesicles of 1,2-dipalmitoyl-, 1,2-dimyristoyl-, and 1-palmitoyl-2-oleoyl-phosphatidylcholine (DPPC, DMPC, and POPC, respectively) under anaerobic conditio...

journal_title：Biophysical journal

authors： Hresko RC,Sugár IP,Barenholz Y,Thompson TE

更新日期：1987-05-01 00:00:00

abstract：:The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho- and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (Lo or lipid...

journal_title：Biophysical journal

authors： Abbandonato G,Storti B,Tonazzini I,Stöckl M,Subramaniam V,Montis C,Nifosì R,Cecchini M,Signore G,Bizzarri R

更新日期：2019-02-05 00:00:00

abstract：:Using homonuclear (1)H NOESY spectra, with chemical shifts, (3)JH(N)H(α) scalar couplings, residual dipolar couplings, and (1)H-(15)N NOEs, we have optimized and validated the conformational ensembles of the amyloid-β 1-40 (Aβ40) and amyloid-β 1-42 (Aβ42) peptides generated by molecular dynamics simulations. We find t...

journal_title：Biophysical journal

authors： Ball KA,Phillips AH,Wemmer DE,Head-Gordon T

更新日期：2013-06-18 00:00:00

abstract：:Photosystem II catalyzes photosynthetic water oxidation in plants, green algae, and cyanobacteria. The manganese-containing active site cycles through a series of five oxidation states, S(n), where n refers to the number of oxidizing equivalents stored. In this report, reaction-induced Fourier transform infrared and e...

journal_title：Biophysical journal

authors： Halverson KM,Barry BA

更新日期：2003-10-01 00:00:00

abstract：:Immunocytochemical, biochemical, and molecular genetic studies indicate that apolipoprotein E (apoE) plays an important role in the process of amyloidogenesis-beta. However, there is still no clear translation of these data into the pathogenesis of amyloidosis-beta. Previous studies demonstrated sodium dodecyl sulfate...

journal_title：Biophysical journal

authors： Golabek AA,Kida E,Walus M,Perez C,Wisniewski T,Soto C

更新日期：2000-08-01 00:00:00

abstract：:Differential scanning calorimetry provides a new window into the plasma proteome. Plasma from normal individuals yields a characteristic, reproducible thermogram that appears to represent the weighted sum of denaturation profiles of the most abundant constituent plasma proteins. Plasma from diseased individuals yields...

journal_title：Biophysical journal

authors： Garbett NC,Miller JJ,Jenson AB,Chaires JB

更新日期：2008-02-15 00:00:00

abstract：:beta-Lactamase, which catalyzes beta-lactam antibiotics, is prototypical of large alpha/beta proteins with a scaffolding formed by strong noncovalent interactions. Experimentally, the enzyme is well characterized, and intermediates that are slightly less compact and having nearly the same content of secondary structur...

journal_title：Biophysical journal

authors： Vijayakumar S,Vishveshwara S,Ravishanker G,Beveridge DL

更新日期：1993-12-01 00:00:00

abstract：:Using polarography and suitable mathematical analysis, the oxygen permeability of an individual layer of a bilayered tissue may be determined if the oxygen consumption and permeability of the other layer are known. Results obtained on rabbit cornea indicate that the permeability of the epithelium is about four times t...

journal_title：Biophysical journal

authors： Freeman RD,Fatt I

更新日期：1972-03-01 00:00:00

abstract：:To probe the complexity of the cell membrane organization and dynamics, it is important to obtain simple physical observables from experiments on live cells. Here we show that fluorescence correlation spectroscopy (FCS) measurements at different spatial scales enable distinguishing between different submicron confinem...

journal_title：Biophysical journal

authors： Wawrezinieck L,Rigneault H,Marguet D,Lenne PF

更新日期：2005-12-01 00:00:00

abstract：:We characterized the effect of the first basepair on the conformational dynamics of the fluorescent dye Cy3 attached to the 5' end of double-stranded DNA using gaussian-mixture adaptive umbrella sampling simulations. In the simulations, the sampling of all five dihedral angles along the linker was enhanced, so that bo...

journal_title：Biophysical journal

authors： Spiriti J,Binder JK,Levitus M,van der Vaart A

更新日期：2011-02-16 00:00:00

abstract：:Amyloid diseases are traditionally characterized by the appearance of inter- and intracellular fibrillar protein deposits, termed amyloid. Historically, these deposits have been thought to be the etiology of the disease. However, recent evidence suggests that small oligomers of the amyloidogenic protein/peptide are th...

journal_title：Biophysical journal

authors： Ding H,Wong PT,Lee EL,Gafni A,Steel DG

更新日期：2009-08-05 00:00:00

abstract：:Pure Michaelis-Menten enzymes have been studied (i.e., enzymes with a hyperbolic (S, V) behavior in a well-stirred solution). When such enzymes are associated with a structure in vitro, even in the simplest conceivable form (immobilization in a homogeneous gel), they can produce enzymic or transport reactions with man...

journal_title：Biophysical journal

authors： Vincent JC,Thellier M

更新日期：1983-01-01 00:00:00

abstract：:The excess oxygen concentration in the photosynthetic membranes of functioning oxygenic photosynthetic cells was estimated using classical diffusion theory combined with experimental data on oxygen production rates of cyanobacterial cells. The excess oxygen concentration within the plesiomorphic cyanobacterium Gloeoba...

journal_title：Biophysical journal

authors： Kihara S,Hartzler DA,Savikhin S

更新日期：2014-05-06 00:00:00

abstract：:A new microscopic technique is demonstrated that combines attributes from both near-field scanning optical microscopy (NSOM) and fluorescence resonance energy transfer (FRET). The method relies on attaching the acceptor dye of a FRET pair to the end of a near-field fiber optic probe. Light exiting the NSOM probe, whic...

journal_title：Biophysical journal

authors： Vickery SA,Dunn RC

更新日期：1999-04-01 00:00:00

abstract：:Myoplasmic free calcium transients were measured with antipyrylazo III in voltage clamped segments of frog skeletal muscle fibers and were used to calculate the rate of release (Rrel) of calcium from the sarcoplasmic reticulum. Intramembrane charge movement was measured for the same pulses in the same fibers. During a...

journal_title：Biophysical journal

authors： Simon BJ,Schneider MF

更新日期：1988-12-01 00:00:00

abstract：:Activation of the photoreceptor G protein transducin (Gt) by opsin, the ligand-free form of rhodopsin, was measured using rod outer segment membranes with densities of opsin and Gt similar to those found in rod cells. When GTPgammaS was used as the activating nucleotide, opsin catalyzed transducin activation with an e...

journal_title：Biophysical journal

authors： Melia TJ Jr,Cowan CW,Angleson JK,Wensel TG

更新日期：1997-12-01 00:00:00

abstract：:A mesoscale model of DNA is presented (3SPN.1), extending the scheme previously developed by our group. Each nucleotide is mapped onto three interaction sites. Solvent is accounted for implicitly through a medium-effective dielectric constant and electrostatic interactions are treated at the level of Debye-Hückel theo...

journal_title：Biophysical journal

authors： Sambriski EJ,Schwartz DC,de Pablo JJ

更新日期：2009-03-04 00:00:00

abstract：:Exceptions to the Meyer-Overton rule are commonly cited as evidence against indirect, membrane-mediated mechanisms of general anesthesia. However, another interpretation is possible within the context of an indirect mechanism in which solubilization of an anesthetic in the membrane causes a redistribution of lateral p...

journal_title：Biophysical journal

authors： Cantor RS

更新日期：2001-05-01 00:00:00

abstract：:Scaffold proteins are central players in regulating the spatial-temporal organization of many important signaling pathways in cells. They offer physical platforms to downstream signaling proteins so that their transient interactions in a crowded and heterogeneous environment of cytosol can be greatly facilitated. Howe...

journal_title：Biophysical journal

authors： Su Z,Dhusia K,Wu Y

更新日期：2020-11-17 00:00:00